共查询到20条相似文献,搜索用时 15 毫秒
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Bonar E Dubin A Bierczyńska-Krzysik A Noga M Silberring J Stalińska K Koj A 《Cytokine》2006,33(2):111-117
Interleukin-1 (IL-1) and interleukin-6 (IL-6) are principal proinflammatory cytokines inducing the acute phase response of various tissues, including liver. Cultured human hepatoma HepG2 cells were stimulated with IL-1 (10 ng/ml) and IL-6 (10 ng/ml). After 24 h the cells were collected and disrupted by sonication in a lysis buffer containing 8M urea. The extracted cellular proteins were separated by 2D polyacrylamide gel electrophoresis. The gels were stained with Coomassie Brilliant Blue R-250 and the protein spots showing different intensities in comparison to control (unstimulated) cells were excised and subjected to analysis by LC-MS/MS. Alternatively, proteins were stained with SYPRO Ruby. These differentially expressed proteins include seven up-regulated and two down-regulated intracellular proteins of various functions. The identification of three cytokine-responsive proteins was confirmed by biosynthetic labeling with [35S]methionine after incubation of HepG2 cells, and by western blot with specific antisera. 相似文献
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New asthma drugs acting on gene expression 总被引:5,自引:1,他引:4
Popescu FD 《Journal of cellular and molecular medicine》2003,7(4):475-486
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Expression of novel cytokine transcripts in the murine placenta 总被引:3,自引:0,他引:3
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Analysis of interleukin-18, interleukin-1 converting enzyme (ICE) and interleukin-18-related cytokines in Crohn's disease lesions 总被引:4,自引:0,他引:4
Pagès F Lazar V Berger A Danel C Lebel-Binay S Zinzindohoué F Desreumaux P Cellier C Thiounn N Bellet D Cugnenc PH Fridman WH 《European cytokine network》2001,12(1):97-104
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P. Anil Kumar P. Swathi Chitra Chunxia Lu J. Sobhanaditya Ram Menon 《Journal of physiology and biochemistry》2014,70(2):433-440
Adipose tissue remodeling in obesity involves macrophage infiltration and chronic inflammation. NF-kB-mediated chronic inflammation of the adipose tissue is directly implicated in obesity-associated insulin resistance. We have investigated the effect of growth hormone (GH) on NF-kB activity in preadipocytes (3T3-F442A) and macrophages (J774A.1). Our studies indicate that whereas GH increases NF-kB activity in preadipocytes, it decreases NF-kB activity in macrophages. This differential response of NF-kB activity to GH correlates with the GH-dependent expression of a cadre of NF-kB-activated cytokines in these two cell types. Activation of NF-kB by GH in preadipocytes heightens inflammatory response by stimulating production of multiple cytokines including TNF-α, IL-6, and MCP-1, the mediators of both local and systemic insulin resistance and chemokines that recruit macrophages. Our studies also suggest differential regulation of miR132 and SIRT1 expression as a mechanism underlying the observed variance in GH-dependent NF-kB activity and altered cytokine profile in preadipocytes and macrophages. These findings further our understanding of the complex actions of GH on adipocytes and insulin sensitivity. 相似文献
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