蓝蓟的组织培养与快速繁殖

1 植物名称蓝蓟(Echium VUlgare L.). 2 材料类别茎段和茎尖. 3 培养条件基本培养基为MS.(1)诱导芽萌发培养基:MS+6-BA 0.5 mg·L-1(单位下同)+NAA 0.1.(2)增殖培养基:MS+6-BA 1.0+NAA 0.1;(3)生根培养基:MS+IBA 0.5+NAA 0.05.     

水栒子的组织培养与快速繁殖

1 植物名称 水枸子(Cotoneaster multiflorus Bunge). 2 材料类别 茎尖和茎段. 3 培养条件 基本培养基为MS.诱导培养基:(1)MS+6-BAl.0 mg-L-1(单位下同)+NAA 0.2;继代培养基:(2)MS+6.BA 0.5+NAA 0.5,(3)MS+6-BA0.1+NAA 0.1;生根培养基:(4)1/2MS+IBA 0.5+NAA 0.1.     

光叶楮的组织培养和快速繁殖

1植物名称光叶楮(Broussonetia papyrifera),又名构树. 2材料类别茎尖与茎段. 3培养条件启动培养基:(1)MS 6-BA 1.0 mg·L-1(单位下同) NAA 0.5.增殖培养基:(2)MS 6-BA0.5 NAA 0.02;(3)MS 6-BA 1.0 NAA 0.2;(4)MS 6-BA 1.5 NAA 0.1;(5)MS 6-BA 1.5 NAA 0.5.生根培养基:(6)1/2MS IBA 0.5;(7)1/2MS NAA0.3;(8)MS 6-BA 0.2 NAA 0.01.以上培养基均添加3%蔗糖、0.7%琼脂,pH 5.8.培养温度25～28℃,光照12 h·d-1,光照度2 000lx.     

花叶日本醉鱼草的微型快繁

1植物名称花叶日本醉鱼草(Buddleja japonica‘Variegata'). 2材料类别茎尖和带腋芽的茎段. 3培养条件启动培养基:(1)MS 6-BA 0.05mg·L-1(单位下同) IBA 0.05 NAA 0.05.增殖培养基:(2)MS 6-BA 0.1 NAA 0.1;(3)MS 6-BA 0.2 NAA 0.1;(4)MS 6-BA 0.5 NAA 0.1.生根培养基:(5)MS IAA 0.1 IBA 0.05.以上培养基均加入3%蔗糖、0.65%琼脂,pH 5.8.培养温度为(23±1)℃,光照时间12 h·-1,光照度1 000～1 500 lx.     

大花萱草新品种"金娃娃"的组织培养和快速繁殖

1 植物名称大花萱草(Hemerocallis middendorfii)新品种金娃娃. 2 材料类别茎尖. 3 培养条件芽诱导培养基:(1)MS+ NAA 0.5 mg*L-1 (单位下同)+6-BA 0.1;(2)MS+IBA 0.5;(3)MS+6-BA 1.0+IBA 1.5.生根培养基:(4)MS+IBA 0.2;(5)MS+NAA 0.5;(6)1/2MS+NAA 0.2.以上培养基均加入0.55%琼脂,3%蔗糖,pH 5.8,培养温度22～26℃,光照度2 000～2 500 lx,光照14 h*d-1.     

灰毡毛忍冬的组织培养与快速繁殖

1植物名称灰毡毛忍冬(Lonicera macranthoides Hand.Mazz),别名大花忍冬. 2材料类别茎尖、带腋芽茎段. 3培养条件芽诱导与继代增殖培养基:(1)MS 6-BA 0.3 mg·L-1(单位下同) NAA 0.3;(2)MS 6-BA 0.3 NAA 0.5;(3)MS 6-BA 0.3 NAA 0.8;(4)MS 6-BA0.3 NAA 1.2;(5)MS 6-BA 0.5 NAA 0.3;(6)MS 6-BA 0.8 NAA 0.3;(7)MS 6-BA 1.2 NAA 0.3;(8)MS KT 0.5 NAA 0.3.生根培养基:(9)1/2MS NAA0.4 IBA 1.0.培养基加0.8%琼脂;(9)中加2%蔗糖,(1)～(8)中加3%蔗糖;pH 5.8.培养温度(25±1)℃,光照度1 200 lx,光照时间12 h·d-1.     

金芯丝兰的组织培养与快速繁殖

1植物名称金芯丝兰(Yucca.filamentosa). 2材料类别茎尖. 3培养条件(1)启动培养基:MS 6-BA 2 mg·L-1(单位下同) NAA 0.5 蔗糖3%;(2)继代增殖培养基:MS 6-BA 5 NAA 0.2 蔗糖3%;(3)生根培养基:1/2MS NAA 0.1 2%蔗糖.上述培养基均加琼脂0.7%,pH 5.8.培养温度为(25 2)℃,光照度为2 000 lx,光照时间12 h·d-1.     

凉粉草的组织培养及快速繁殖

1植物名称凉粉草(Mesona chinensis Benth.). 2材料类别茎尖及带腋芽的茎段. 3培养条件基本培养基为MS.(1)丛芽分化培养基:MS 6-BA 1.0 mg·L-1(单位下同) NAA 0.1;(2)增殖培养基:MS 6-BA 0.5 NAA 0.1;(3)生根培养基:MS NAA 0.5.以上培养基均附加3%白糖、0.7%琼脂,pH 5.8.培养温度为25℃左右,光照时间14 h·d-1,光强为20～30 μmol·m-2·s-1.     

芙蓉葵的离体培养与植株再生

1 植物名称 芙蓉葵(Hibiscus moscheutos Linn.). 2 材料类别 带节茎段. 3 培养条件 以MS为基本培养基.(1)芽诱导培养基:MS+6-BA 2.0 mg-L-1(单位下同)+NAA 0.1;(2)增殖培养基:MS+6-BA 1.0+NAA 0.05:(3)生根培养基:1/2MS+NAA 1.0.     

峨眉姜花离体培养与快速繁殖(简报)

以峨眉姜花地下茎茎尖为外植体,在MS+6-BA 8.0~10.0 mg/L+NAA 0.2 mg/L培养基上可诱导不定芽,在MS+6-BA 4.0 mg/L+NAA 0.2 mg/L+3%蔗糖培养基上进行增殖培养,在1/2 MS+IBA 0.5 mg/L+2%蔗糖培养基上进行生根培养后移栽入珍珠岩:泥炭(1:1)的混合基质中,成活率达87%。     

Investigation of the mechanism of temperature sensitivity of the electroreceptors of ampullae of lorenzini

In experiments on Black Sea skates (Raja clavata), the potential of the receptor epithelium of the ampullae of Lorenzini and spike activity of single nerve fibers connected to them were investigated during electrical and temperature stimulation. Usually the potential within the canal was between 0 and –2 mV, and the input resistance of the ampulla 250–400 k. Heating of the region of the receptor epithelium was accompanied by a negative wave of potential, an increase in input resistance, and inhibition of spike activity. With worsening of the animal's condition the transepithelial potential became positive (up to +10 mV) but the input resistance of the ampulla during stimulation with a positive current was nonlinear in some cases: a regenerative spike of positive polarity appeared in the channel. During heating, the spike response was sometimes reversed in sign. It is suggested that fluctuations of the transepithelial potential and spike responses to temperature stimulation reflect changes in the potential difference on the basal membrane of the receptor cells, which is described by a relationship of the Nernst's or Goldman's equation type.I. P. Pavlov Institute of Physiology, Academy of Sciences of the USSR, Leningrad. I. M. Sechenov, Institute of Evolutionary Physiology and Biochemistry, Academy of Sciences of the USSR, Leningrad. Pacific Institute of Oceanology, Far Eastern Scientific Center, Academy of Sciences of the USSR, Vladivostok. Translated from Neirofiziologiya, Vol. 12, No. 1, pp. 67–74, January–February, 1980.     

Principles of organization and evolution of systems of regulation of functions

Evolution of living organisms is closely connected with evolution of structure of the system of regulations and its mechanisms. The functional ground of regulations is chemical signalization. As early as in unicellular organisms there is a set of signal mechanisms providing their life activity and orientation in space and time. Subsequent evolution of ways of chemical signalization followed the way of development of delivery pathways of chemical signal and development of mechanisms of its regulation. The mechanism of chemical regulation of the signal interaction is discussed by the example of the specialized system of transduction of signal from neuron to neuron, of effect of hormone on the epithelial cell and modulation of this effect. These mechanisms are considered as the most important ways of the fine and precise adaptation of chemical signalization underlying functioning of physiological systems and organs of the living organism