共查询到20条相似文献,搜索用时 31 毫秒
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Design of an optimal promoter involved in the heat‐induced transcriptional pathway in Arabidopsis,soybean, rice and maize
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Kyonoshin Maruyama Takuya Ogata Norihito Kanamori Kyouko Yoshiwara Shingo Goto Yoshiharu Y. Yamamoto Yuko Tokoro Chihiro Noda Yuta Takaki Hiroko Urawa Satoshi Iuchi Kaoru Urano Takuhiro Yoshida Tetsuya Sakurai Mikiko Kojima Hitoshi Sakakibara Kazuo Shinozaki Kazuko Yamaguchi‐Shinozaki 《The Plant journal : for cell and molecular biology》2017,89(4):671-680
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Inhibition of the activation of heat shock factor in vivo and in vitro by flavonoids. 总被引:17,自引:0,他引:17
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N Hosokawa K Hirayoshi H Kudo H Takechi A Aoike K Kawai K Nagata 《Molecular and cellular biology》1992,12(8):3490-3498
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Positive and negative regulation of basal expression of a yeast HSP70 gene. 总被引:21,自引:9,他引:12
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The SSA1 gene, one of the heat-inducible HSP70 genes in the yeast Saccharomyces cerevisiae, also displays a basal level of expression during logarithmic growth. Multiple sites related to the heat shock element (HSE) consensus sequence are present in the SSA1 promoter region (Slater and Craig, Mol. Cell. Biol. 7:1906-1916, 1987). One of the HSEs, HSE2, is important in the basal expression of SSA1 as well as in heat-inducible expression. A promoter containing a mutant HSE2 showed a fivefold-lower level of basal expression and altered kinetics of expression after heat shock. A series of deletion and point mutations led to identification of an upstream repression sequence (URS) which overlapped HSE2. A promoter containing a mutation in the URS showed an increased level of basal expression. A URS-binding activity was detected in yeast whole-cell extracts by a gel electrophoresis DNA-binding assay. The results reported in this paper indicate that basal expression of the SSA1 promoter is determined by both positive and negative elements and imply that the positively acting yeast heat shock factor HSF is responsible, at least in part, for the basal level of expression of SSA1. 相似文献
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