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A complementary DNA oligomer releases a transcription pause complex 总被引:10,自引:0,他引:10
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Tanaka H Fujita K Sagisaka A Tomimoto K Imanishi S Yamakawa M 《Molecular biotechnology》2009,41(2):173-179
RNAi knockdown by using shRNA expression plasmids is widely used to determine the function of individual genes in mammals. Here we developed a simple method to create an IR DNA in a U6 small nuclear RNA promoter-based parent vector using a single-stranded IR DNA with short hairpin structure and Bst DNA polymerase. Furthermore, we demonstrated that the shRNA expression plasmids constructed by our method effectively induced target-specific RNAi in the silkworm cell line. We also found that sequence preference in the silkworm cell line was much lower than in mammalian cells and shRNA-induced RNAi was influenced by the length of the stem region. 相似文献
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