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Characterization and structure of genes for proteases A and B from Streptomyces griseus. 总被引:14,自引:8,他引:6
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Protease A and protease B are extracellular proteins which are secreted by Streptomyces griseus. The genes encoding protease A (sprA) and protease B (sprB) were isolated from an S. griseus genomic library by using a synthetic oligonucleotide probe. Fragments containing sprA and sprB were characterized by hybridization and demonstration of proteolytic activity in Streptomyces lividans. Each DNA sequence contains a large open reading frame with the coding region of the mature protease situated at its carboxy terminus. The amino terminus of each reading frame appears to encode a 38-amino-acid signal peptide followed by a 76- or 78-amino-acid polypeptide, a propeptide, which is joined to the mature protease. Strong homology between the coding regions of the protease genes suggests that sprA and sprB originated by gene duplication. 相似文献
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Ohnishi Y Yamazaki H Kato JY Tomono A Horinouchi S 《Bioscience, biotechnology, and biochemistry》2005,69(3):431-439
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