Polarization-Dependent Resonance Light Scattering of Biomolecular Layer Coated Gold Nanoshell

Polarization-dependent resonance light scattering (RLS) of biomolecular layer coated gold nanoshell are investigated theoretically by means of the quasistatic approximation. Both the intensity and wavelength of RLS are sensitive to the azimuth angle and can be tuned by altering the core dielectric constant and biomolecular layer thickness. In the direction parallel to the incident polarization, RLS could be enhanced by decreasing the core dielectric constant or increasing the layer thickness whereas, in the direction perpendicular to the incident polarization, the RLS is only sensitive to the core dielectric constant. The variation of RLS corresponding to the changing of biomolecular layer thickness also greatly depends on the polarization. The variation of RLS intensity always reaches its maximum when the azimuth angle is 0 and can be improved by increasing the gold shell thickness or decreasing the core dielectric constant. However, the variation of RLS wavelength always reaches its maximum when the azimuth angle is between 0 and π/2 and can be improved by decreasing the gold shell thickness or core dielectric constant. This optimization of polarization-dependent RLS response of gold nanoshell to the biocoating is potentially useful in biosensing applications.     

Plasmon Resonances in V-Shaped Gold Nanostructures

Using numerical simulations, we examine the change in plasmon resonance behavior in gold nanorod structures that have a V shape. The reduction in symmetry compared to linear rods causes two different longitudinal-type resonances to appear in a single structure, and the relative intensity and hybridization of these can be controlled by varying the angle of the arms of the ??V.?? The resonances may also be selectively excited by controlling the polarization of the incident light, thereby providing a convenient way to control a nanoscale optical electric field using far-field parameters. For example, the wavelength at which a strong resonance occurs in the V-shaped structures studied can be switched between 630 and 900?nm by a 90° rotation of the polarization of the incident light. Due to the symmetry of the targets, there will be three types of special near-field location; a location at which the electric field intensity is enhanced by either resonance, a location at which the electric field intensity is enhanced by the 630?nm resonance but not by the 890?nm resonance, and a location at which the electric field intensity is enhanced by the 890?nm resonance but not by the 630?nm one.     

Plasmonic Lens with Multiple-Turn Spiral Nano-Structures

In this paper, we investigate the focusing properties of a plasmonic lens with multiple-turn spiral nano-structures, and analyze its field enhancement effect based on the phase matching theory and finite-difference time-domain simulation. The simulation result demonstrates that a left-hand spiral plasmonic lens can concentrate an incident right-hand circular polarization light into a focal spot with a high focal depth. The intensity of the focal spot could be controlled by altering the number of turns, the radius and the width of the spiral slot. And the focal spot is smaller and has a higher intensity compared to the incident linearly polarized light. This design can also eliminate the requirement of centering the incident beam to the plasmonic lens, making it possible to be used in plasmonic lens array, optical data storage, detection, and other applications.     

Characterization of Grating Coupled Surface Plasmon Polaritons Using Diffracted Rays Transmittance

A method to sense the excitation of surface plasmon polariton (SPP) on metallic grating device using the transmitted signal will be presented. The grating transmittance signal will be fully characterized varying the light incident angle and azimuthal grating orientation by means of the SPP vector model and rigorous coupled-wave analysis simulation. Simulation results will be compared with experimental measurements obtained with a 635 nm wavelength laser in the transverse magnetic polarization mode. The laser will light grating devices in contact with either air or water through a customized microfluidic chamber. A characterization of the diffracted rays will show the relationship between the grating coupling configuration and the Kretschmann one. In fact, the diffracted ray affected by SPP resonance is transmitted with an output angle which is the same incident angle that should be used to excite SPP in Kretschmann configuration. Lastly, the grating parameters (amplitude and metal thickness) impact on transmittance signal will be analyzed with respect to the order zero reflectance signal.     

Polarization Splitter with Optical Bistability in Metal Gap Waveguide Nanocavities

A polarization splitter is proposed and numerically investigated. It is composed of two same structures with different arrangement, which is a kind of metal-dielectric nanocavity filling a piece of nonlinear optical material into metal gap waveguides for each. This device with optical bistability based on surface plasmon provides a new way to manipulate light by tuning the incident light intensity and will be essential for the coming optical information processes.     

In vivo measurement of glucose concentration with lasers.

Common polarimeters defined the concentration of an optically active sample by measuring the intensity of the light beam after it has passed through the analyser. Consequently, it can be stated that up to now, there have been the disadvantages of having a signal dependent on absolute light intensity. The new method that is described has a sensitivity that is in great measure independent of absolute light intensity, whereby only one light trace is necessary. The new principle uses no mechanical rotations. Instead, an electrical signal indicates the amount of optical rotation of the sample. The high sensitivity that can be reached is theoretically only limited by polarization noise. By going to the uttermost physical and electronic lengths, sensitivity values of more than 10(-5) degrees can be reached. Furthermore, the mechanical dimensions of the apparatus can be made very small by the application of a solid-state laser.     

Laser light-scattering studies of bull spermatozoa. I. Orientational effects.

Calculations based on the known dimensions of bull spermatozoa show that the scattered light intensity is strongly dependent upon the relative orientation of the particle to the incident beam. The magnitude of this effect of apparently much greater than for other systems where motility has been investigated by dynamic light scattering. The calculations show that the scattering source can be approximated by a small spinning mirror, and consequently the greatest light intensity at the detector results from cells swimming in a direction perpendicular to the scattering vector. The calculations are in substantial agreement with photographic observations, as well as direct measurements of the scattered intensity. Previous treatments of dynamic light scattering from swimming bull spermatozoa based on point scattering models are shown to be incorrect.     

The polarization of fluorescence of DNA stains depends on the incorporation density of the dye molecules.

BACKGROUND: The fluorescence induced by polarized light sources, such as the lasers that are used in flow cytometry, is often polarized and anisotropic. In addition, most optical detector systems are sensitive to the direction of polarization. These two factors influence the accuracy of fluorescence intensity measurements. The intensity of two light sources can be compared only if all details of the direction and degree of polarization are known. In a previous study, we observed that fluorescence polarization might be modified by dye-dye interactions. This report further investigates the role of dye density in fluorescence polarization anisotropy. METHODS: We measured the polarization distribution of samples stained with commonly used DNA dyes. To determine the role of fluorophore proximity, we compared the monomeric and a dimeric form of the DNA dyes ethidium bromide (EB), thiazole orange (TO), and oxazole yellow (YO). RESULTS: In all dyes sampled, fluorescence polarization is less at high dye concentrations than at low concentrations. The monomeric dyes exhibit a higher degree of polarization than the dimeric dyes of the same species. CONCLUSIONS: The polarization of fluorescence from DNA dyes is related to the density of incorporation into the DNA helix. Energy transfer between molecules that are in close proximity loosens the linkage between the excitation and emission dipoles, thereby reducing the degree of polarization of the emission.     

Cross-polarized reflected light measurement of fast optical responses associated with neural activation

We developed an optical probe for cross-polarized reflected light measurements and investigated optical signals associated with electrophysiological activation in isolated lobster nerves. The cross-polarized baseline light intensity (structural signal) and the amplitude of the transient response to stimulation (functional signal) measured in reflected mode were dependent on the orientation of the nerve axis relative to the polarization plane of incident light. The maximum structural signal and functional response amplitude were observed at 45 degrees , and the ratio of functional to structural signals was approximately constant across orientations. Functional responses were measured in single trials in both transmitted and reflected geometries and responses had similar waveforms. Both structural and functional signals were an order of magnitude smaller in reflected than in transmitted light measurements, but functional responses had similar signal/noise ratios. We propose a theoretical model based on geometrical optics that is consistent with experimental results. In the model, the cross-polarized structural signal results from light reflection from axonal fibers and the transient functional response arises from axonal swelling associated with neural activation. Polarization-sensitive reflected light measurements could greatly enhance in vivo imaging of neural activation since cross-polarized responses are much larger than scattering signals now employed for dynamic functional neuroimaging.     

Polarization Effect on Superfocusing of a Plasmonic Lens Structured with Radialized and Chirped Elliptical Nanopinholes

Tuning effect of different polarization states was presented in this paper. It can be realized by a plasmonic lens constructed with elliptical pinholes ranging from submicron to nanoscales distributed in variant period along radial direction. Propagation properties of the lens illuminated under four different polarization states: linear, elliptical, radial, and cylindrical vector beam, were calculated and analyzed combining with finite-difference time-domain algorithm. Different focusing performances of the lens were illustrated while the polarized light passes through the pinholes. Our calculation results demonstrate that polarization effect of the elliptical pinholes-based plasmonic lens can generate high transmission intensity and sharp focusing for our proposed specific structures. Beam focal region, position, and transmission intensity distribution can be tailored by the four polarization states.     

Calculations of scattered light from rigid polymers by Shifrin and Rayleigh-Debye approximations.

We show that the commonly used Rayleigh-Debye method for calculating light scattering can lead to significant errors when used for describing scattering from dilute solutions of long rigid polymers, errors that can be overcome by use of the easily applied Shifrin approximation. In order to show the extent of the discrepancies between the two methods, we have performed calculations at normal incidence both for polarized and unpolarized incident light with the scattering intensity determined as a function of polarization angle and of scattering angle, assuming that the incident light is in a spectral region where the absorption of hemoglobin is small. When the Shifrin method is used, the calculated intensities using either polarized or unpolarized scattered light give information about the alignment of polymers, a feature that is lost in the Rayleigh-Debye approximation because the effect of the asymmetric shape of the scatterer on the incoming polarized electric field is ignored. Using sickle hemoglobin polymers as an example, we have calculated the intensity of light scattering using both approaches and found that, for totally aligned polymers within parallel planes, the difference can be as large as 25%, when the incident electric field is perpendicular to the polymers, for near forward or near backward scattering (0 degrees or 180 degrees scattering angle), but becomes zero as the scattering angle approaches 90 degrees. For randomly oriented polymers within a plane, or for incident unpolarized light for either totally oriented or randomly oriented polymers, the difference between the two results for near forward or near backward scattering is approximately 15%.     

Optical polarization properties of the diffraction spectra from single fibers of skeletal muscle.

The diffraction spectra of laser light from single fibers of skeletal muscle exhibit a large degree of optical depolarization. When the linearly polarized incident laser source is oriented at polarization angles between 0 less than theta less than pi/2 rad with respect to the fiber axis, the diffracted light is elliptically polarized. These results show that the phase angle of the ellipse rotates by as much as 20 degrees when the fiber is stretched from 2.4 to 3.8 microns. To further ascertain that the observed phenomenon is diffraction related, an experiment monitoring the spectra of scattered light in between diffraction orders showed this signal to be significantly more linearly polarized. These results suggest that the degree of elliptical polarization of the diffraction spectra is a sensitive probe of A-band dynamics, including changes of the anisotropic S-2 elements.     

Polarized light orientation in honey bees: Is time a component in sampling?

Bees on a horizontal comb can orient their dances by a field of polarized light in the zenith even when the degree of polarization of this light field is modulated from 0 to 100%, at frequencies between 0.05 and 25 Hz, with the direction of polarization and the intensity kept constant. The result suggests that bees use a process of polarized light evaluation which probes simultaneously with three or more differently oriented analyser channels. It would follow that, in this experimental situation, time is not a component of sampling.     

Detection of polarization and direction by the bee rhabdom

Summary Light propagates along the bee rhabdom in the form of patterns known as modes. The modes convey information about the polarization and direction of the incident light. We show theoretically, using electromagnetic theory, that the fine structure of the worker bee rhabdom can detect this polarization and directional information, and that the rhabdomeres do not all have the same field of view for off axis light.Queen Elizabeth II Fellow.We thank Professor G. A. Horridge and Simon Laughlin for their assistance with this study.     

Assessment of the potential of the blue light gradient in soybean pulvini as a leaf orientation signal

Blue light gradients in the pulvini of soybean ( Glycine max var. Northrup King S1346) leaves with different laminar orientations were examined with a fiber optic microprobe. The gradients changed markedly as a function of both incident light angle and leaf position and were determined largely by the amount of light present in the adaxial side of the pulvinus. The steepest gradient for inclined leaves was with light incident at 90° whereas for declined leaves it occurred when the light was incident at 150°. A proposed pulvinar mechanism which allows for the detection of light direction and leaf position by using the blue light gradient as an orientation signal could account for solar tracking by soybean.     

Far-Field Focusing of Spiral Plasmonic Lens

In this paper, we study the nanoscale-focusing effect in the far field for a spiral plasmonic lens with a concentric annular groove by using finite-difference time domain simulation. The simulation result demonstrates that a left-hand spiral plasmonic lens can concentrate an incident right-hand circular polarization light into a focal spot at the exit surface. And this spot can be focused into far field due to constructive interference of the scattered light by the annular groove. The focal length and the focal depth can be adjusted by changing the groove radius and number of grooves within a certain range. These properties make it possible to probe the signal of spiral plasmonic lens in far field by using conventional optical devices.     

Complex Polarization Response in Plasmonic Nanospirals

Archimedean nanospirals exhibit many far-field resonances that result from the lack of symmetry and strong intra-spiral plasmonic interactions. Here, we present a computational study, with corroborating experimental results, on the plasmonic response of the 4π Archimedean spiral as a function of incident polarization, for spirals in which the largest linear dimension is less than 550 nm. We discuss the modulation of the near-field structure for linearly and circularly polarized light in typical nanospiral configurations. Computational studies of the near-field distributions excited by circularly polarized light illustrate the effects of chirality on plasmonic mechanisms, while rotation of linearly polarized light provides a detailed view of the effects of broken symmetry on nanospiral fields in any given direction in the plane of the spiral. The rotational geometry exhibits a preference for circular polarization that increases near-field enhancement compared to excitation with linearly polarized light and exchanges near-field configurations and resonant modes. By analyzing the effects of polarization and wavelength on the near-field configurations, we also show how the nanospiral could be deployed in applications such as tunable near-field enhancement of nonlinear optical signals from chiral molecules.     

Optical Bifacial Transmission by Asymmetric Charge-Oscillation-Induced Light Transmission Through a Plasmonic Structure

From first-principles computation, we reveal that optical bifacial transmission can be induced within an asymmetric metallic subwavelength structure. This phenomenon can be explained by a concrete picture in which the intensity of the driving forces for surface plasmon or charge wave is asymmetric for the two incident directions. Two distinguished different numerical methods, finite difference time domain (FDTD), and rigorous coupled wave analysis (RCWA) are utilized to verify that optical bifacial transmission can exist for linear plasmonic metamaterial. Previous results are also reviewed to confirm the physical meaning of optical bifacial transmission for a planar linear metamaterial. The incident light can provide direct driving forces for surface plasmon in one direction. While in the opposite direction, forces provided by the light diffraction are quite feeble. With the asymmetric driving forces, the excitation, propagation, and light-charge conversion of surface plasmon give the rise of bifacial charge-oscillation-induced transmission. In periodic a structure, the excitation of surface plasmon polariton can lead to the spoof vanish of such phenomenon. The transmissions for two incident directions get the same in macroscopic while the bifacial still exists in microscale.     

Effects of incident light intensity and light path length on cell growth and oil accumulation in Botryococcus braunii (Chlorophyta)

Botryococcus braunii was cultured in different light path length under different incident light intensity to investigate the effect of light on alga growth as well as hydrocarbon and fatty acid accumulation. Results indicated that longer light path length required higher incident light intensity in order to meet the light requirement of algal growth and hydrocarbon accumulation during the course of cultivation. However, hydrocarbon profile was only affected by the incident light intensity and not influenced by the light path length. High incident light intensity enhanced the accumulation of hydrocarbons with longer carbon chains. Besides, the fatty acid content and profiles were significantly influenced by both incident light intensity and light path. Higher fatty acid content and higher percentage of C18 and monounsaturated fatty acid components were achieved at the higher incident light intensity and lower light path length. Taken together, these results are benefit to improve its biomass and oil productivity through the optimization of light and photobioreactor design.