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1.
The anamorph genus Metarhizium is composed of arthropod pathogens, several with broad geographic and host ranges. Members of the genus, including "M. anisopliae var. frigidum" nomen nudum and Metarhizium flavoviride, have been used as biological insecticides. In a recent revision of the genus the variety "M. anisopliae var. frigidum" was suggested to be a synonym of M. flavoviride based largely on ITS sequence phylogenetic analysis. In this study we conducted morphological evaluations and multigene phylogenetic analyses with EF-1alpha, RPB1 and RPB2 for strains of M. flavoviride and "M. anisopliae var. frigidum." Included in these evaluations were the ex-type of M. flavoviride var. flavoviride and what likely would be considered the "ex-type' of the invalidly published taxon "M. anisopliae var. frigidum". Based on morphological and molecular evidence we conclude that "M. anisopliae var. frigidum" is distinct from M. flavoviride and the taxon M. frigidum sp. nov. is described. 相似文献
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Distribution of a phosphoenolypyruvate-dependent sugar phosphotransferase system in mycoplasms 总被引:4,自引:6,他引:4
A survey of 10 mycoplasma strains has shown that their capacity to accumulate radioactivity from alpha-methyl-d-glucopyranoside depends on the activity of a phosphoenolpyruvate-dependent phosphotransferase system (PTS), and that this system endows the organisms with a high affinity for glucose as a fermentation substrate. PTS activity was found in Mycoplasma gallisepticum, M. mycoides var. mycoides, and M. mycoides var. capri, but in none of the fermentative Acholeplasma strains nor in some of the nonfermentative Mycoplasma species. Partial characterization of the PTS of M. mycoides var. capri has shown that, like the PTS of Escherichia coli and Staphylococcus aureus, it is strictly dependent on phosphoenolpyruvate as a phosphoryl donor and on componenets of both the cytoplasm and the membrane. 相似文献
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The efficacy of a new virulent Metarhizium anisopliae variety (M. anisopliae var. dcjhyium, DQ288247) obtained from Odontotermes formosanus in China was evaluated against the subterranean termite, O. formosanus, in the laboratory. The new variety was compared with four other virulent M. anisopliae isolates and was found to be highly infectious and virulent against termites. M. anisopliae var. dcjhyium could cause approximately 100% mortality of termites 3 days post-inoculation in the concentration of 3x10(8) conidia/ml. There were also differences in relative hyhal growth and isoenzymes. M. anisopliae var. dcjhyium showed a different isoenzyme band pattern from the four isolates of M. anisopliae (AB027337, AB099510, AB099941 and AF280631). The phylogenetic tree of the 18S rDNA sequences revealed the taxonomic and evolutionary position of M. anisopliae var. dcjhyium. M. anisopliae var. dcjhyium and four isolates of M. anisopliae formed a monophyletic group, supported by a 99% bootstrap value. M. anisopliae var. dcjhyium formed a distinct variety, which had a special characterization of unique bands of isoenzyme, high virulence and low repellency against termites when compared with four other isolates of M. anisopliae. 相似文献
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对国产黄杨科5 种植物作了分类学修订或补充: 将雀舌黄杨归入匙叶黄杨, 中间黄杨归入黄杨, 多毛板凳果归入板凳果, 双蕊野扇花归入羽脉野扇花; 恢复黄杨和尖叶黄杨的原学名;报道宜昌黄杨在湖南的新记录。 相似文献
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The complete nuclear rDNA gene complex of Metarhizium anisopliae var. anisopliae isolate ME1 is 8118bp long and contains the 18S, 5.8S, and 28S rRNA genes as well as the ITS and IGS regions. Variation in the ITS of isolates of M. anisopliae var. anisopliae and one each of Metarhizium anisopliae var. acridum, Metarhizium flavoviride var. flavoviride, and Metarhizium flavoviride var. minus, clustered 39 out of 40 of M. anisopliae var. anisopliae isolates in one clade. Nucleotide sequence variation in the IGS among 21 of M. anisopliae var. anisopliae isolates showing IGS length variation sorted them into three strongly supported clades, which were weakly correlated with insect hosts and were not correlated with geographic location. Two group-I introns, Ma-int4 and Ma-int5, were discovered in the 18S and the 3(') end of the 28S, in M. anisopliae var. anisopliae isolates ITALY-12 and IMBST 9601. The insertion sites and sub-group of these introns correlated with their closest relatives, as judged by phylogenetic analysis of intron nucleotide sequence. 相似文献
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国产黄杨科六种植物的考订 总被引:4,自引:0,他引:4
林祁 《热带亚热带植物学报》1999,7(1):31-33
对国产黄杨科6种植物作了修订或补充:将头花黄杨归入匙叶黄杨,平卧皱叶黄杨、越橘叶黄杨和矮生黄杨归入皱叶黄杨,汕头黄杨归入狭叶黄杨,东方野扇花归入长叶柄野扇花。报道了杨梅黄杨在湖北、江西和福建的新分布,皱叶黄杨在广西和福建的新分布,双蕊野扇花在贵州和广西的新分布。 相似文献
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Mycoplasma taxonomy studiedy electrophoresis of cell proteins 总被引:18,自引:4,他引:14
S Razin 《Journal of bacteriology》1968,96(3):687-694
The electrophoretic patterns of cell proteins in polyacrylamide gels were used for the study of several taxonomic problems in the Mycoplasmatales. The patterns of five Mycoplasma hominis strains showed marked differences that corresponded with their known serological and nucleic acid heterogeneity. The patterns of three M. mycoides var. mycoides strains isolated in different countries were essentially identical. The electrophoretic patterns of several caprine strains resembled those of M. mycoides var. mycoides, supporting their classification as M. mycoides var. capri. Strain B3, a swine isolate, accordingly was tentatively identified as M. mycoides var. capri. The bovine mastitis strain M. agalactiae var. bovis possessed a pattern basically similar to that of the goat mastitis strain M. agalactiae, supporting the inclusion of both strains in one species. Three M. pulmonis strains isolated from rats or tissue cultures showed nearly identical patterns. The pattern of the toxigenic M. neurolyticum (Sabin A) strain resembled but was not identical with that of the nontoxigenic PG28 strain. The avian Mycoplasma species, M. gallisepticum, M. meleagridis, M. synoviae, M. gallinarum, and M. iners showed easily distinguishable and specific patterns, supporting their present classification in different species. Several improvements in the electrophoretic technique are described, and its advantages and limitations as a taxonomic tool are discussed. 相似文献
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Markku HÄKKINEN Chee How TEO Yasmin Rofina OTHMAN 《植物分类学报》2007,45(1):69-74
Borneo is a large island in Southeast Asia. It is divided politically into three parts: the kingdom of Brunei on the north central coast; the Malaysian states of Sarawak and Sabah to the west and east; with Kalimantan of Indonesia making up the larger par… 相似文献
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描述了泰国雨久花属Monochoria C.Presl一新变种:窄叶鸭舌草M.vaginalis var.angustifolia G.X.Wang。该新变种与原变种鸭舌草M.vaginalis var.vaginalis都具有类似的总状花序,但前者的叶片为窄披针形,3-7×0.3-2.0cm,叶片宽长比在0.1-0.4之间,叶基部裂片最长不超过2mm,总状花序具花3-7朵,而原变种鸭舌草的叶片较宽,为卵心形或心形,4-9×2-8cm,叶片宽长比在0.5-0.95之间,叶基部裂片最长可达到2cm,总状花序 相似文献
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P. D. Bridge C. Prior J. Sagbohan C. J. Lomer M. Carey A. Buddie 《Biodiversity and Conservation》1997,6(2):177-189
The relationships between 30 strains of Metarhizium were investigated by isoenzyme analysis, PCR-RAPDs, and protease production. The strains included representatives of M. anisopliae, M. flavoviride var. flavoviride and M. flavoviride var. minus. Thirteen isolates conforming to M. flavoviride from acridoid hosts and pathogenic to locusts and grasshoppers were shown to be a single, distinctive genotype with a pan-tropical distribution. In addition, the separation of M. flavoviride var. minus as a host specific taxon distinct from European isolates of M. flavoviride was also supported. The possibility of coevolution within some insect pathogenic populations is discussed. 相似文献
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绿僵菌大孢变种的生物学特征及其对蛴螬的毒力研究 总被引:10,自引:0,他引:10
以报道较多的蛴螬病原真菌 布氏白僵菌、绿僵菌小孢变种作参照菌,系统研究了新分离鉴定的绿僵菌大孢变种的生物学特性,测定了其在偏低的自然气温下对蛴螬的致病能力.结果表明,绿僵菌大孢变种与参照菌的培养要求接近,最适宜的温度为25 ℃.3种供试的培养基中,PPDA为最适宜的培养基,其次为SDAY.在偏低的温度环境下绿僵菌大孢变种对蛴螬的毒杀能力强于布氏白僵菌,土壤和喷雾处理后累计僵虫率分别达88.23%和76.47%,有很好的田间应用前景. 相似文献
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The diatom family Rhaphoneidaceae is characterized by high generic diversity and low species diversity with most genera known to have long stratigraphic ranges. The genera within this family are neritic marine, and mostly epipsammic. A new modern and epipsammic genus, Meloneis gen. nov., is described herein and is compared to all genera within Rhaphoneidaceae and especially to Rhaphoneis Ehrenberg s.l. Within Meloneis three new species and one variety are distinguished and described herein: M. mimallis sp. nov., M. mimallis var. zephyria var. nov., M. akytos sp. nov., and M. gorgis sp. nov. 相似文献
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This paper deals with the chromosome morphology of three wild eggplants (Solanum indicum L., S. indicum L. var. recurvatum C. Y. Wu et S. C. Huang,S. coagulans Forsk.) grown in Shi-shuang-banna and two cultivars (S. melongena L.var. serpentinum Bailey, S. melogena L. var. esculantum Nees). All their chromosomenumbers are 2n=24. The chromosome idiograms of wild eggplants axe as follows: Solanum indicum,2G+12J+2I+8M; S. indicum var. recurvatum, 8G+12J+2I+2M; S. coagulans ( No. 20),10J+2I+12M; S. coagulans(No. 21), 8J+14M+2L; and those of cultivars are: S. melongena var. serpentinum, 6G+12J+2I+4M; S. melongena var. esculantum, 12J+2I+10M. The chromosome idiograms among three wild eggplants are quite different. However, they are rather closely related in each other between the two cultivars. Butthe relative length and the relative position of the sat-chromosomes in each chromo)meidiogram are conspicuously variable. 相似文献
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龙骨星蕨与膜叶星蕨的关系:来自叶绿体rbcL、rps4和trnL-trnF、rps4-trnS序列的证据 总被引:1,自引:0,他引:1
龙骨星蕨(Microsoriummembranaceumvar. carinatum)是新近发表的一个变种,从形态特征看,它与原变种膜叶星蕨(M. membranaceumvar. membranaceum)可以很容易地区分。但将龙骨星蕨与另外 3种国产星蕨属 (Mi crosorium)植物叶绿体基因组中rbcL、rps4基因和trnL trnF、rps4 trnS基因间隔区进行PCR扩增和序列分析,并与已经发表的真蕨类的相应序列进行比较发现,龙骨星蕨与膜叶星蕨在所研究的 4个DNA片段中均未表现出序列差异(除了trnL trnF区的一个 2bp长度差异外 ),而所比较的其他真蕨类变种间的相应DNA片段均存在一定的差异。因此DNA序列证据不支持龙骨星蕨变种的成立。 相似文献
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为理清芸香科(Rutaceae)九里香属(Murraya)中大叶九里香[M. kwangsiensis (C. C. Huang)C. C. Huang var. macrophylla C. C. Huang]与其原变种广西九里香[M. kwangsiensis(C. C. Huang)C. C. Huang var. kwangsiensis]间的系统关系及其分类学地位,采用综合文献查阅、标本鉴定、野外实地调查、栽培观察及显微解剖等多种方法对九里香属植物数个种类进行分类学相关研究。结果发现:大叶九里香在叶片、花、果实等形态特征方面与广西九里香间的差异极大,故将其处理为一个独立的种,并提出新等级组合Murraya macrophylla (C. C. Huang)F. J. Mou; 首次对大叶九里香的形态特征及分布进行详尽描述; 该物种的羽状复叶及小叶均为九里香属中最大者,更接近仅分布于越南的光叶九里香[M. glabra (Guillaumin)Swingle],但其较大的小叶、叶面叶脉下凹且叶片皱缩等特征使其明显不同于九里香属其他种类。大叶九里香是一个独立种而非广西九里香的变种,此关于大叶九里香分类地位的新观点有助于整个九里香属的系统发育关系重建及种间系统关系的深入探索。 相似文献