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轮状病毒(Rotavirus RV)是引起大熊猫幼兽顽固性腹泻的重要病原之一,患病大熊猫病死率较高,是大熊猫种群减少的一个重要威胁。目前此病毒已经引起兽医工作者的高度重视,本文对大熊猫轮状病毒的研究现状进行综述。 相似文献
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总结了十余年大熊猫生殖轴系垂体促性腺细胞,睾丸,卵巢,精子,卵子和受精机理以及生殖激素测定经验,分析了大熊猫生殖能力低的根本原因是生殖内分泌失调,提出了“双控”措施,即对雌性大熊猫采用补充外源促性腺激素,促进滤泡发育,卵子成熟和发情排卵,而对雄性大熊猫,采用了人工采精,精液冷却保存的方法;采用“双控”措施的六年里圈养大熊猫空怀明显下降,获双胎7次,单胎9次,出现了大熊猫繁殖史上空前繁荣的大好形势。 相似文献
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大熊猫垂体泌乳素(PRL)cDNA的克隆与表达 总被引:9,自引:0,他引:9
从大熊猫脑垂体中提取总RNA,利用RT-PCR技术,首次扩增出大熊猫垂体泌乳素(PRL)cDNA序列(GenBank登录号:AY161285)。结果表明,大熊猫PRL cDNA的开放阅读框为687bp,编码含229个氨基酸残基的前体蛋白。将克隆的PRL cDNA片段构建到表达质粒pGEX-4T-1中,转化大肠杆菌BL21,在异丙基硫代半乳糖苷(IPTG)的诱导下表达PRL蛋白。SDS—PAGE电泳结果表明,表达的PRL蛋白为不可溶蛋白。蛋白质同源性比较显示,大熊猫与猪、猫的同源性大于95%,与人、牛、山羊的在70%~80%之间,与小鼠、大鼠的分别为45.9%和52%。大熊猫aa^64为丝氨酸,是亲水性氨基酸;而猫、牛、山羊(脯氨酸)和人(丙氨酸)aa^64是疏水性氨基酸。在二级结构上,aa^64在第一α螺旋与第二α螺旋之间,极性的差别可能导致蛋白空间结构的不同。 相似文献
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圈养大熊猫种群的动态及发展趋势 总被引:2,自引:0,他引:2
采用直线回归方程、4次方程和指数增长式微分方程等数理分析方法,对全世界1953~2003年圈养大熊猫种群的发展趋势进行了分析.发现对大熊猫的圈养种群数量建立的回归方程是拟合很好的4次多项式方程,1953~1987年的圈养种群增长曲线成S型,1998年后的种群增长成直线或指数式.依此进行预测,从1998年以后只依靠现有饲养种群167只就可能使种群数量稳定增长,全世界种群数量2007年可突破200只,2009年可能达到220~240只,大概在15~22年后全世界饲养种群数量就能够翻一番,达到330多只. 相似文献
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A. J. E. Cave 《Journal of Zoology》1974,172(1):123-131
The bursa epipharyngea of the Giant panda, Ailuropoda melanoleuca, is a saccular diverticulum (sacculus epipharyngeus) situated between pharynx roof and basis cranii. In the Ursidae the bursa comprises a pair of unequal-sized diverticula having separate ostial communication with the epipharynx. Such dual type of bursa was recorded by Davis (1964) in the Ailuropoda specimen he systematically anatomized and was regarded as evidence indicating affinity with the Ursidae rather than with the Procyonidae. But in four additional Ailuropoda specimens subsequently examined, and described herein, the ursid pattern of sacculus did not obtain. The significance of this difference in the anatomical findings is indicated. 相似文献
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The faecal flora of the giant panda (Ailuropoda melanoleuca) 总被引:2,自引:0,他引:2
The faecal floras of two adult (male and female) and one infant (male) giant panda kept at the Ueno Zoo, Tokyo, Japan were examined and shown to be quite different from those of other animals. The predominant bacteria in the adults were Streptococcus (including Enterococcus) and Enterobacteriaceae, while obligate anaerobes had minor populations. Fastidious anaerobes were not detected. The predominant bacteria in the suckling infant were Lactobacillus and Streptococcus, followed by Bifidobacterium. After the infant began to eat bamboo leaves the number of Lactobacillus decreased and Bifidobacterium became undetectable, whereas Enterobacteriaceae became one of the most predominant flora. The most dominant streptococcus isolated from the female panda was identified as Streptococcus bovis, but those from the male adult and the weaned infant were not identified as any known species. 相似文献
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结合已公布的大熊猫Ailuropoda melanoleuca基因组和本实验室所测6只大熊猫的转录组数据,筛选多态性微卫星位点并分析其组成及特征。结果显示:共获得326个多态性微卫星位点,其中二碱基多态性微卫星最多,共228个,占69.93%;三、四、五、六碱基所占比例分别为9.51%、14.11%、5.21%、1.22%。根据分析结果中缺失率与标准差2项指标以及位点序列长度,选取20个多态性二碱基微卫星位点,用于25只大熊猫个体血液DNA进行PCR验证并做后续分析。结果表明:不同位点的等位基因数为2~8,平均等位基因数为3.70,观测杂合度、期望杂合度分别为0~1.000、0.280~0.784,平均值分别为0.472和0.532。在Bonferroni校正后,证实4个位点显著偏离哈迪-温伯格平衡,所有位点未观察到显著连锁不平衡(P>0.01)。20个位点多态信息含量(PIC)在0.246~0.734,其中具有高度多态性的位点9个(PIC>0.50),11个位点呈中度多态性(0.25相似文献
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K. Hirayama S. Kawamura T. Mitsuoka† K. Tashiro‡ 《Journal of applied microbiology》1989,67(4):411-415
The faecal floras of two adult (male and female) and one infant (male) giant panda kept at the Ueno Zoo, Tokyo, Japan were examined and shown to be quite different from those of other animals. The predominant bacteria in the adults were Streptococcus (including Enterococcus ) and Enterobacteriaceae, while obligate anaerobes had minor populations. Fastidious anaerobes were not detected. The predominant bacteria in the suckling infant were Lactobacillus and Streptococcus , followed by Bifidobacterium. After the infant began to eat bamboo leaves the number of Lactobacillus decreased and Bifidobacterium became undetectable, whereas Enterobacteriaceae became one of the most predominant flora. The most dominant streptococcus isolated from the female panda was identified as Streptococcus bovis , but those from the male adult and the weaned infant were not identified as any known species. 相似文献
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中国保护大熊猫研究中心雅安碧峰峡基地的一只大熊猫患皮肤病,从皮屑中分离到一株拟盘多毛孢菌GYMXP201108。进行了形态学观察和ITS序列分析,并构建系统进化树。结果表明,该菌株顶端附属丝短,1–3根,多数不具柄,与海南拟盘多毛孢Pestalotiopsis hainanensis模式菌株形态一致,ITS区序列分析也表明与海南拟盘多毛孢的序列相似性最高,为99.6%,因此鉴定为海南拟盘多毛孢,GenBank ID为KF551573。首次报道了海南拟盘多毛孢能在动物体表生存。 相似文献
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A. J. E. CAVE 《Journal of Zoology》1975,176(4):567-576
Observations are submitted upon the anatomy of the thyroid and parathyroid glands of Ailuropoda melanoleuca following gland investigation in three specimens. Variations of thyroid configuration and of parathyroid topography are noted, the thyroid vasculature is described in detail and an initial contribution is made to thyroid and parathyroid histology in this genus. 相似文献
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本文以2010—2020年15只母兽带仔野化培训的大熊猫幼仔为研究对象,基于红外视频监控系统观察和音频颈圈解译获得的行为资料、GPS颈圈跟踪定位采集的粪样数据,分析了野化培训大熊猫幼仔的行为发育进程和食性转换特征。结果表明:随着野化培训大熊猫幼仔的生长发育,与觅食和警戒相关的行为得到充分发育,且具有较强的时间关联性,包括食乳、爬行、走动、玩耍物品、爬树、咬玩竹子、饮水和采食竹子等。8~10月龄的大熊猫幼仔开始取食竹子,其发育性食性转换过程划分为3个阶段:食乳期(1~7月龄)、食母乳—食竹子转换期(8~28月龄)和食竹期(29~39月龄),其中转换期细分为关键期(8~18月龄)和过渡期(19~28月龄)。从统计检验来看,不同食性阶段间差异显著;过渡期的大熊猫幼仔可离开母兽独立生活,此阶段大熊猫幼仔食物组分比例与食竹期相比无显著差异。野化培训大熊猫幼仔的季节性食性转换规律与带仔母兽和野生大熊猫具有相似的格局,即春季主要取食竹笋,夏、秋季则以嫩竹茎和竹叶为食,冬季采食竹叶与竹茎。 相似文献