Incisor growth rate in rodents and the record of the entire annual cycle in the incisors of Marmota baibacina centralis

The lower incisors of 80 specimens of Marmota baibacina of 1?C10 years old killed in spring in the highland of the Tien Shan Mountains were investigated. On the surface of all the incisors, regular daily increments and ??hibernation zones?? were revealed. The mean width of increments was used as an indicator of the incisor growth rate before hibernation. We found that the mean width did not depend on the sex of animals, locality, and incisor length, but depended on animal age. The increment width significantly decreased, and the number of the increments formed before hibernation significantly increased with age. This means that the growth rate of the incisors and the intensity of their attrition decrease with the animals?? age. The decrease in the incisor growth rate with age is a manifestation of the well-known decrease with age in the intensity of all growth processes in mammals. As a result of this decrease, in the majority of old marmots under study, zones of hibernation were revealed in the basal as well as in the apical parts of the incisor. The zone in the basal part formed during the latest hibernation and the zone in the apical part corresponded to the previous one. Thus, the incisor preserved the record of the entire year of animal life.     

A record of hibernation on the surface of incisor teeth in the hamster Mesocricetus raddei (Nehring, 1894)

The surface of the lower incisor teeth was studied in an adult male Mesocricetus raddei hamster with a temperature logger implanted into its peritoneal cavity in June 2010. From July 2010 to March 2011, the hamster lived under natural conditions in an enclosure and overwintered in a self-made burrow. Logger data showed that, beginning from mid-August, the animal body temperature periodically dropped and then returned to the norm, with the duration and depth of hypothermia (torpor bouts) increasing and those of nor-mothermia (arousals) decreasing with time. Growth increments on the incisor surface (presumably, daily increments) were unclear in the apical and middle tooth parts but very distinct in the basal part. The number and width of basal increments generally corresponded to the number and duration of arousals. The growth of the incisors apparently continued throughout the winter season, with every arousal after a torpor bout being marked by the formation of a distinct increment on the incisor surface. Thus, it appears that the pattern of increments on the incisor surface can be used to evaluate the course of wintering in hibernating hamsters. Experiments with vital markers are needed to confirm this assumption.     

The effects of day length, hibernation, and ambient temperature on incisor dentin in the Turkish hamster (Mesocricetus brandti)

Dentin is deposited on a circadian basis, and daily layers manifest as bands on the medial surfaces of rodent incisors. Hibernation alters dentin deposition, and a distinct hibernation mark has been described on incisor surfaces of several rodent species; the factors that influence the morphology of this mark are poorly understood. We tested the effects of day length, torpor expression, and ambient temperature on incisor surface morphology in Turkish hamsters housed in one of four conditions: long days (LDs) at 22 °C, short days (SDs) at 22 °C, SDs at 5 °C, and SDs at 13 °C. Body temperature was monitored continuously with implanted radio transmitters, and teeth examined postmortem. Teeth of SD hamsters had narrower, less distinct circadian increments than those of LD hamsters, but the width of ultradian increments was similar in both photoperiods. Hibernation at both 5 and 13 °C was associated in most specimens with very narrow, sharply defined dentin increments and increased tooth heterogeneity. Hamsters in SDs at 5 °C that did not hibernate lacked characteristic hibernation increments. At 5 °C, but not 13 °C, the number and cumulative width of hibernation increments were related to number and cumulative duration of periodic arousals. Our results suggest that incremental deposition of dentin in rodent incisors may be a useful trait for characterizing hibernation behavior in both evolutionary and historical contexts.     

Daily Increments on the Incisor Surface in Rodents: Intra-and Interspecific Variations

Investigation of the incisors of 17 rodent species from 11 genera revealed substantial interspecific and intraspecific variations in the pattern of the daily increment on the incisor surface. The morphology of daily increments is described in detail. Interspecific variations in the pattern are correlated with interspecific variations in the daily activity rhythms in rodents. Intraspecific (up to individual) traits can be attributed to intraspecific (up to individual) differences in daily activity. We can conclude that the daily activity of an individual determines the pattern of its incisor growth rhythm, not being a direct cause of daily increment formation. Thus, the pattern of the increment on the incisor surface can be considered as recording the rhythm of the daily activity of a rodent during the period of incisor renewal.     

Disproportionate eruption of maxillary and mandibular incisors in the long-tailed ground squirrel

The surface of the maxillary and mandibular incisors of Spermophilus undulatus long-tailed ground squirrels, including those born in the current year and those that have hibernated (trapped one month or later after hibernation) is studied. The presence of daily growth increments on the incisors’ surface allows the evaluation of the eruption rate of the incisors; a specific change in the character of the growth increments corresponds to winter hibernation (hibernation zone), which serves as the time mark. Ratio between the eruption rates of the maxillary and mandibular incisors typical for rodents is found in young-of-the-year and some animals after hibernation. In these animals the eruption rate of the mandibular incisors is higher than the eruption rate of the maxillary incisors and can be taken as proportional to their length. In individuals that have hibernated and show proportional eruption of the incisors, the proportions of the total length of the incisor formed before hibernation zone are equal for the maxillary and mandibular incisors. In the individuals that also have hibernated and show the ratio between the total length of the maxillary and mandibular incisors typical for rodents, the eruption rate of the mandibular incisor is equal to or less than the eruption rate of the maxillary incisor and the proportion of the incisor formed before hibernation is greater in the mandibular incisor than in the maxillary. This disproportionate pattern of incisor eruption is not typical for rodents and is a result of inequal attrition of the maxillary and mandibular incisors, which ultimately results in the normal ratio of the total length of the maxillary and mandibular incisors.     

Dental fast track: Prenatal enamel growth,incisor eruption,and weaning in human infants

Correlation between the timing of permanent first molar eruption and weaning age in extant primates has provided a way to infer a life history event in fossil species, but recent debate has questioned whether the same link is present in human infants. Deciduous incisors erupt at an age when breast milk can be supplemented with additional foods (mixed feeding), and weaning is typically complete before permanent first molars erupt. Here, I use histological methods to calculate the prenatal rate by which enamel increases in thickness and height on human deciduous incisors, canines, and molars (n = 125). Growth trajectories for each tooth type are related to the trimesters and assessed against the eruption sequence and final crown height. Analyses show that central incisors initiate early in the second trimester with significantly faster secretion rates relative to canines and second molars, which initiate closer to birth. Even though initial extension rates were correlated with crown height and scaled with positive allometry within each tooth class, the relatively short incisors still increased in height at a significantly faster rate than the taller canines and molars. The incisor prenatal “fast track” produces a greater proportion of the crown before birth than all other tooth types. This growth mechanism likely facilitates early incisor eruption at a time when the mixed feeding of infants can be initiated as part of the weaning process. Findings provide a basis from which to explore new links between developmental trends along the tooth row and mixed feeding age in other primates. Am J Phys Anthropol 156:407–421, 2015. © 2014 Wiley Periodicals, Inc.     

Alterations in the incisor development in the Tabby mouse

The X-linked tabby (Ta) syndrome in the mouse is homologous to the hypohidrotic ectodermal dysplasia (HED) in humans. As in humans with HED, Ta mice exhibit hypohidrosis, characteristic defects of hairs and tooth abnormalities. To analyze the effects of Ta mutation on lower incisor development, histology, morphometry and computer-aided 3D reconstructions were combined. We observed that Ta mutation had major consequences for incisor development leading to abnormal tooth size and shape, change in the balance between prospective crown- and root-analog tissues and retarded cytodifferentiations. The decrease in size of Ta incisor was observed at ED13.5 and mainly involved the width of the tooth bud. At ED14.5-15.5, the incisor appeared shorter and narrower in the Ta than in the wild type (WT). Growth alterations affected the diameter to a greater extent than the length of the Ta incisor. From ED14.5, changes in the shape interfered with the medio-lateral asymmetry and alterations in the posterior growth of the cervical loop led to a loss of the labio-lingual asymmetry until ED17.0. Although the enamel organ in Ta incisors was smaller than in the WT, a larger proportion of the dental papilla was covered by preameloblasts-ameloblasts. These changes apparently resulted from reduced development of the lingual part of the enamel organ and might be correlated with a possible heterogeneity in the development of the enamel organ, as demonstrated for upper incisors. Our observations suggest independent development of the labial and lingual parts of the cervical loop. Furthermore, it appeared that the consequences of Ta mutation could not be interpreted only as a delay in tooth development.     

Epidermal growth factor-induced precocious incisor eruption is associated with decreased tooth size

Epidermal growth factor (EGF) causes precocious eruption of incisors in vivo and is mitogenic for tooth-derived cells in vitro. These two observations lead to the hypothesis that the EGF-induced precocious eruption is the result of an increase in the size of the incisor. To test this hypothesis, neonatal mice were injected daily with various doses of EGF and, seven days after birth, were perfused with fixative. EGF causes a retardation of overall growth (as measured by body weight) and a dose-dependent thickening of the epidermis. The incisors were examined in midsagittal histological sections and in X-ray microradiographs. Contrary to our expectations, EGF causes a dose-dependent decrease in the size of the incisors. This result suggests that the stimulation of the growth of odontogenic cells seen in tissue culture is not part of the physiological response to EGF in vivo and that EGF-induced precocious eruption of incisors is not due to an increase in the growth rate of the tooth.     

The use of otolith microstructure to estimate age in adult Atlantic cod Gadus morhua

Transverse sections of otoliths from Atlantic cod Gadus morhua from the Baltic Sea revealed narrow growth increments. The widths of these increments corresponded to daily increments from fish with known otolith growth rates and were therefore assumed to be daily increments. They exhibited a distinct pattern with increasing distance from the primary primordium. A series of zones with clearly distinguishable increments, first with increasing then with decreasing widths in a dome‐shaped pattern, were separated by zones where no regular increment structure was visible. Increment width seemed to be tightly coupled to the annual cycle in environmental temperature at a depth of 30–60 m, where G. morhua predominantly reside. Between 135 and 200 increments occurred within the different zones, with a non‐significant trend towards lower increment numbers and widths with distance from the primary primordium of the otolith. Increment formation apparently ceased at temperatures < 5–6° C, but growth during the cold months corresponded closely with estimated growth rates. The increment patterns seemed to reflect annual cycles in environmental temperature, and the count of the increment cycles may thus be a promising tool for the determination of the true age of Baltic G. morhua.     

Relationship of incisor size to diet and anterior tooth use in sympatric sumatran anthropoids

Researchers often relate anthropoid incisor size to diet and ingestive behaviors. It is suggested that primates that frequently consume large, tough foods (i.e., fruits) require large incisors to process these items. This idea has been difficult to test because of a lack of data on anterior tooth use in wild primates, and a lack of understanding concerning the relationships between food properties and ingestive behaviors. The first field study of primate ingestive behaviors has recently been completed for four species of Sumatran anthropoids: Hylobates lar, Macaca fascicularis, Pongo pygmaeus, and Presbytis thomasi [Ungar, American Journal of Physical Anthropology 95:197–219, 1994; International Journal of Primatology 16:221–245, 1995]. This paper documents both relative and absolute incisor row width differences among these taxa, and evaluates the relationships between incisor size and feeding behaviors for specific taxa. Results indicate that differences in incisor size among these species cannot all be explained by degree of frugivory, food item size, or even degree of incisor use in ingestion alone. It is therefore suggested that inferences of dietary differences based on largely or solely on differences in incisor sizes of specific fossil anthropoid taxa should be approached with caution. © 1996 Wiley-Liss, Inc.     

On the growth of incisors in ground squirrels (Spermophilus) during hibernation

The growth of incisors was studied in two Spermophilus parryii and two S. undulatus ground squirrels with DS-1922L temperature data loggers implanted in the peritoneal cavity, which were kept under laboratory conditions. Daily increments on the incisors surface were similar to those in other species of ground squirrels, but they were less distinct and regular than in wild-living conspecific individuals from the same region. Two S. parryii and one S. undulatus ground squirrels entered hibernation and successfully overwintered. Despite some anomalies in their incisors, changes in body temperature during hibernation (recorded by the data loggers) had an effect on the pattern of their growth, resulting in the formation of a “hibernation zone” on the incisor surface. The number of narrow increments within this zone roughly corresponded to the number of alternating periods of torpor and euthermia during hibernation. This could be regarded as evidence that the incisors of the animals studied continued growing throughout hibernation, including the period of deep hibernation, with the rhythm of their growth coinciding with the rhythm of changes in body temperature. The effect of spontaneous trauma of an upper incisor on the growth of other incisors is described.     

The topographical anatomy of the incisor canal]

In 254 skulls (22-70 years of age) with orthognathic intact occlusion in 66 maxillodental sawing topographic anatomy of the incisor canal has been studied by means of craniometric and statistical methods. In mature age the average length of the incisor canal and its openings possess certain statistically significant age and sex differences. The incisor canal is situated near the tops of the central incisors roots at the distance of 7.40 +/- 0.11 mm. The incisor opening, independently on the sex, is situated on the inferior surface of the palatile process of the median line at the distance of 9.81 +/- 0.21 mm from the point formed by the medial angles of the crowns of the upper central incisors. In mature age the distance between the incisor opening and the central incisors roots is 3.51 +/- 0.10 mm.     

The Morphology and Periodic Structures of the Otolith of the Chinook Salmon (Oncorhynchus tshawytscha), and Temperature-dependent Variation in Otolith Microscopic Growth Increment Width

The otolith (sagitta) of the chinook salmon (Oncorhynchus tshawytscha) has a variable external crystalline morphology which is related to differences in the growth rate of crystals in different parts of the otolith. The internal crystal structure of the otolith is complex with different mineral phases in different growth fields of the otolith and a well-defined series of microscopic growth increments in both the dorsal and the ventral parts (orientation in situ) of the otolith. The period of the microscopic growth increments was shown, by both a rearing experiment and interpolation from fish of known age, to be daily. By rearing sibling chinook salmon at different temperatures (while still maintaining them on the same diet) it was shown that the width of the daily growth increment varies with temperature, but neither multi- nor sub-daily increments appear in the area of regular, daily growth incrementation. Inclusions of the vaterite morph of calcium carbonate crystallizing in the botryoidal habit occur in the otherwise aragonitic otolith. Regularly spaced check rings in the sulcul part of the otolith are homologous with those occurring in the dorsal and ventral growth axis and are. on average, separated by about 28 microincrements.     

Validation of daily increment deposition and early growth of mud carp Cirrhinus molitorella

In this study, laboratory reared Cirrhinus molitorella from hatching through to the juvenile stage were used to validate daily increment deposition. Lapilli were suitable for ageing C. molitorella. The slope of the linear relationships between increment counts and age was not significantly different from 1 (n = 258, P > 0·05), indicating that growth increments are deposited daily. The first increment following the first‐feeding check formed 3 days post hatch (dph). One hundred and twenty‐three juveniles, ranging from 36·4 to 84·7 mm in body length (L_B), were collected in the Pear River estuary from July to January in both 2014 and 2015 and used to estimate growth rate. The core in the lapillus, consisting of a single primordium, a diffuse area and the first‐feeding band, was followed by 20 narrow and poorly contrasted daily increments. Thereafter, increments were wide and well‐defined, and, finally became less distinct, but still homogenous after c. 53 dph. The mean increment width increased to the 29th increment and stayed relatively constant up to the 35th increment and then, gradually declined to the edge of the otolith. Wild juveniles were aged from 69 to 178 dph and hatched from the 15 February to 2 September. Growth rate was estimated from the adjusted linear regression of L_B on age: L_B = 4·37 + 0·44A (r² = 0·60), where A = age. The radius of the core and the regularity and resolution of the increments showed some differences between reared and wild fish. These results are of value for the further study of early life traits and recruitment of C. molitorella.     

MAPK mediates Hsp25 signaling in incisor development

Rodent incisors are continuously growing teeth that include all stages of amelogenesis. Understanding amelogenesis requires investigations of the genes and their gene products control the ameloblast phenotype. One of the mechanisms related to tooth differentiation is mitogen-activated protein kinase (MAPK) signaling. The extracellular-signal regulated kinase (ERK)/mitogen-activated protein kinase kinase (MEK) cascade is associated with mechanisms that control the cell cycle and cell survival. However, the roles of cascades in incisor development remain to be determined. In this study, we investigated incisor development and growth in the mouse based on MAPK signaling. Moreover, heat-shock protein (Hsp)-25 is well known to be a useful marker of odontoblast differentiation. We used anisomycin (a protein-synthesis inhibitor that activates MAPKs) and U0126 (a MAPK inhibitor that blocks ERK1/2 phosphorylation) to examine the role of MAPKs in Hsp25 signaling in the development of the mouse incisor. We performed immunohistochemistry and in vitro culture using incisor tooth germ, and found that phospho-ERK (pERK), pMEK, and Hsp25 localized in developing incisor ameloblasts and anisomycin failed to produce incisor development. In addition, Western blotting results showed that anisomycin stimulated the phosphorylation of ERK, MEK, and Hsp25, and that some of these proteins were blocked by the U0126. These findings suggest that MAPK signals play important roles in incisor formation, differentiation, and development by mediating Hsp25 signaling.     

The effects of spaceflight on the mineralization of rat incisor dentin

The lower incisors of Male Wistar rats flown for 18.5 days on the Soviet Cosmos-1129 Biosatellite were sectioned and chemically analyzed with an electron microprobe in order to determine whether there were specific effects of spaceflight on dentin formation/mineralization. Control tissues were obtained from rats housed under identical conditions in a land-based mock-up of the Biosatellite. The profiles of calcium (Ca), phosphorus (P), and sulfur (S) concentrations in dentin were measured in continuous traverses (1.0 micron intervals) from the pulp to the dentinoenamel junction. The incisor dentin formed during spaceflight had higher than normal (at 1G) concentrations of Ca (+ 10-15%) and P (+ 20-30%), particularly in the temporally youngest tissues within 80 micron of the pulp which had been least affected by secondary mineralization. The S-concentration profiles tended to decrease with tissue age. Fourier analysis (to determine the growth rhythms) revealed abnormal distributional patterns of S in the recently formed dentin from the Flight rats. The sulfur fluctuations in Flight animals alone periodically peaked above the irregular background fluctuations. These observations indicate that spaceflight has measureable effects on dentinogenesis, and they may also bear on the problem of the regulatory role of proteoglycans in mineralization and in the maturation of mineral and matrix moieties in skeletal tissue.     

Otolith formation,microstructure and daily increment validation in juvenile perch Perca fluviatilis

The otoliths of laboratory‐reared larval and juvenile perch Perca fluviatilis of known age were analysed to determine the age of otolith formation and validate the formation of daily increments. There was a linear relationship between number of increments and age in days post‐hatching, although by 82 days post‐hatching daily increment counts underestimated actual age by an average of 5 days. Otolith dimensions in relation to standard length indicated allometric growth of otoliths until completion of yolk absorption, and isometric growth thereafter, up to 82 days post‐hatching.     

Circadian rhythms of locomotor activity and temperature selection in sleepy lizards, <Emphasis Type="Italic">Tiliqua rugosa</Emphasis>

This study examined whether the daily rhythms of locomotor activity and behavioural thermoregulation that have previously been observed in Australian sleepy lizards (Tiliqua rugosa) under field conditions are true circadian rhythms that persist in constant darkness (DD) and whether these rhythms show similar characteristics. Lizards held on laboratory thermal gradients in the Australian spring under the prevailing 12-hour light : dark (LD) cycle for 14 days displayed robust daily rhythms of behavioural thermoregulation and locomotor activity. In the 13-day period of DD that followed LD, most lizards exhibited free-running circadian rhythms of locomotor activity and behavioural thermoregulation. The predominant activity pattern displayed in LD was unimodal and this was retained in DD. While mean levels of skin temperature and locomotor activity were found to decrease from LD to DD, activity duration remained unchanged. The present results demonstrate for the first time that this species’ daily rhythm of locomotor activity is an endogenous circadian rhythm. Our results also demonstrate a close correlation between the circadian activity and thermoregulatory rhythms in this species indicating that the two rhythms are controlled by the same master oscillator(s). Future examination of seasonal aspects of these rhythms, may, however, cause this hypothesis to be modified.