Saccharomyces cerevisiae as anodic biocatalyst for power generation in biofuel cell: influence of redox condition and substrate load

Bio (microbial) fuel cell (microbial fuel cell) with Saccharomyces cerevisiae as anodic biocatalyst was evaluated in terms of power generation and substrate degradation at three redox conditions (5.0, 6.0 and 7.0). Fuel cell was operated in single chamber (open-air cathode) configuration without mediators using non-catalyzed graphite as electrodes. The performance was further studied with increasing loading rate (OLRI, 0.91 kg COD/m³-day; OLRII, 1.43 kg COD/m³). Higher current density was observed at pH 6.0 [160.36 mA/m² (OLRI); 282.83 mA/m² (OLRII)] than pH 5.0 (137.24 mA/m²) and pH 7.0 (129.25 mA/m²). Bio-electrochemical behavior of fuel cell was evaluated using cyclic voltammetry which showed the presence of redox mediators (NADH/NAD⁺; FADH/FAD⁺). Higher electron discharge was observed at pH 6.0, suggesting higher proton shuttling through the involvement of different redox mediators. The application of yeast based fuel cell can be extended to treat high strength wastewaters with simultaneous power generation.     

Morphological alterations of Pseudomonas aeruginosa and Escherichia coli by nocardicin A

Abstract Pseudomonas aeruginosa and Escherichia coli were exposed to nocardicin A, and were subsequently observed with transmission and scanning electron microscopes. Although the nocardicin A-induced morphological alterations such as bulges and spheroplast formations were observed both in P. aeruginosa and E. coli , their positions on the cell surface were different in the two species.     

Effects of inoculation strategy and cultivation approach on the performance of microbial fuel cell using marine sediment as bio-matrix

Aims: To investigate the effects of inoculation strategy and cultivation approach on the performance of microbial fuel cell (MFC). Methods and Results: A dual‐chamber sediment fuel cell was set up fed with glucose under batch condition. At day 30, the supernatant consortium was partly transferred and used as inoculum for the evaluation of cultivation approach. Power output gradually increased to 9·9 mW m^?2 over 180 days, corresponding to coulombic efficiency (CE) of 29·6%. Separated biofilms attached anode enabled power output and CE dramatically up to 100·9 mW m^?2 and over 50%, respectively, whereas the residual sediment catalysed MFC gave a poor performance. MFC catalysed by in situ supernatant consortium demonstrated more than twice higher power than MFC catalysed by the supernatant consortium after Fe(OH)₃ cultivation. However, the re‐generation of biofilms from the latter largely enhanced the cell performance. Conclusions: MFC exhibited a more efficient inducement of electroactive consortium than Fe(OH)₃ cultivation. MFC performance varied depending on different inoculation strategies. Significance and Impact of the Study: This is the first time to study cultivation approach affecting electricity generation. In addition, anodic limitations of mass and electron transfer were discussed through MFC catalysed by sediment‐based bio‐matrix.     

The antimicrobial mechanism of electrochemically activated water against Pseudomonas aeruginosa and Escherichia coli as determined by SDS-PAGE analysis

Aims:  To expose bacteria to anolyte and subsequently investigate the effect of anolyte on the protein profiles of treated bacteria.
Methods and Results:  Proteins were extracted from bacteria treated with different concentrations of anolyte and analysed using SDS-PAGE. Fewer and more faint protein bands were observed for concentrated halide anolyte treated bacteria when compared to untreated bacteria while extra protein bands were observed for bacteria exposed to dilute concentrations.
Conclusions:  The undiluted and the 10⁻¹ dilution of halide derived anolyte was effective in killing the test bacteria. Anolyte caused bacterial death by complete destruction of proteins or by causing oxidative stress which resulted in protein fragmentation.
Significance and Impact of the Study:  The results of this study provide information on the antimicrobial mechanism of anolyte on other bacteria for which the information is currently unavailable.