Stem cell therapy for Parkinson’s disease: where do we stand?

A major neuropathological feature of Parkinsons disease (PD) is the loss of nigrostriatal dopaminergic neuron. Patients exhibit motor symptoms, including bradykinesia, rigidity, and tremor. Neural grafting has been reported to restore striatial dopaminergic neurotransmission and induce symptomatic relief. The major limitation of cell replacement therapy for PD is the shortage of suitable donor tissue. The present review describes the possible sources of cells, including embryonic stem cells and somatic adult stem cells, both of which potentially could be used in cell therapy for PD, and discusses the advantages and disadvantages of each cell type.Our work described in this article was supported by the National Institute of Health, USA, the Swedish Research Council, the Swedish Parkinsons Disease Foundation, the Syskonen Svenssons Foundation, an USAMRMC grant, and an ATV grant     

Stem cell ageing: does it happen and can we intervene?

Adult stem cells have become the focus of intense research in recent years as a result of their role in the maintenance and repair of tissues. They exert this function through their extensive expansion (self-renewal) and multipotent differentiation capacity. Understanding whether adult stem cells retain this capacity throughout the lifespan of the individual, or undergo a process of ageing resulting in a decreased stem cell pool, is an important area of investigation. Progress in this area has been hampered by lack of suitable models and of appropriate markers and assays to identify stem cells. However, recent data suggest that an understanding of the mechanisms governing stem cell ageing can give insight into the mechanism of tissue ageing and, most importantly, advance our ability to use stem cells in cell and gene therapy strategies.     

The cancer stem cell theory: is it correct?

The cancer stem cell hypothesis posits that tumor growth is driven by a rare subpopulation of cells, designated cancer stem cells (CSC). Studies supporting this theory are based in large part on xenotransplantation experiments wherein human cancer cells are grown in immunocompromised mice and only CSC, often constituting less than 1% of the malignancy, generate tumors. Herein, we show that all colonies derived from randomly chosen single cells in mouse lung and breast cancer cell lines form tumors following allografting histocompatible mice. Our study suggests that the majority of malignant cells rather than CSC can sustain tumors and that the cancer stem cell theory must be reevaluated.     

Hysteresis and cell cycle transitions: how crucial is it?

Recently, experiments have shown that cyclin-dependent kinase (CDK) activity exhibits hysteresis in its response to total cyclin when cyclin is made nondegradable and controlled externally. This observation was taken to support mathematical modeling predictions regarding the underlying dynamics of the cell cycle. However, cell cycle dynamics can also be generated by other nonhysteretic mechanisms. To examine the robustness of the hysteretic response of CDK activity to total cyclin, we simulated various cell cycle signal transduction networks, and correlated the dynamics to the response function of CDK activity versus total cyclin. By randomly searching the parameter space, we assessed robustness by estimating the frequency of hysteretic versus nonhysteretic dynamical mechanisms. When the dynamical instabilities were caused by feedback loops in CDK phosphorylation and dephosphorylation or by feedback between cyclin and the CDK inhibitor, the response function of CDK activity versus total cyclin correlated well with the dynamical instabilities. However, when the dynamical instabilities originated from feedback between cyclin and APC-CDH1 or RB-E2F, the response function did not correlate with dynamical instabilities. Thus, although a hysteretic response is neither necessary nor sufficient, it is in general a much more robust mechanism for generating cell cycle dynamics than nonhysteretic mechanisms.     

Quantitative modeling in cell biology: what is it good for?

Recently, there has been a surge in the number of pioneering studies combining experiments with quantitative modeling to explain both relatively simple modules of molecular machinery of the cell and to achieve system-level understanding of cellular networks. Here we discuss the utility and methods of modeling and review several current models of cell signaling, cytoskeletal self-organization, nuclear transport, and the cell cycle. We discuss successes of and barriers to modeling in cell biology and its future directions, and we argue, using the field of bacterial chemotaxis as an example, that the closer the complete systematic understanding of cell behavior is, the more important modeling becomes and the more experiment and theory merge.     

Marlo’s windows: why it is a mistake to ignore hazard resistance in LCA

Purpose

Hazard-resistant materials for homes promise environmental benefits, such as avoided waste and materials for repairs, which can be overlooked by scoping in life-cycle assessment (LCA) approaches. Our motivation for pursuing this research was to see how incorporating these avoided losses in the LCA could impact choices between hazard-resistant and traditional materials.

Methods

Two choices common in home construction were analyzed using an LCA process that incorporates catastrophe modeling to consider avoided losses made possible with hazard-resistant materials. These findings were compared to those based on a similar LCA that did not consider these avoided losses. The choices considered were standard windows vs. windows with impact-resistant glass and standard windows with no opening protection vs. standard windows with impact-resistant storm panels.

Results and discussion

For the window comparisons, the standard products were environmentally preferable when avoided losses from storm events were not considered in the LCA. However, when avoided losses were considered, the hazard-resistant products were environmentally preferable. Considering avoided losses in LCAs, as illustrated by the window choices, can change which product appears to be the environmentally preferable option. Further, as home service life increases, the environmental net benefit of the hazard-resistant product increases.

Conclusions

Our results show the value of an LCA approach which allows more complete scopings of comparisons between hazard-resistant materials and their traditional counterparts. This approach will help translate the impacts of hazard-resistant products into the more familiar language used to talk about “green” products, enabling more informed decisions by product manufacturers, those who develop building certification systems and codes, researchers, and other building industry stakeholders.     

Curvilinear, geometric and phylogenetic modeling of basicranial flexion: is it adaptive, is it constrained?

Prior work has shown that the degree of basicranial flexion among primates is determined by relative brain size, with anatomically modern humans possibly having a less flexed basicranium than expected for their relative brain size. Basicranial flexion has also been suggested to be adaptive in that it maintains a spheroid brain shape, thereby minimizing connections between different parts of the brain. In addition, it has been argued that the degree of flexion might be constrained such that increases in relative brain size beyond that seen in Australopithecus africanus were accommodated by mechanisms other than basicranial flexion. These hypotheses were evaluated by collating an extensive data set on basicranial flexion and relative brain size in primates and other mammals. The data were analyzed using standard least squares regression, geometric and curvilinear modeling, and phylogenetically independent contrasts (PICs). Geometric modeling does not support the hypothesis that flexion is an adaptation that facilitates enlargement of a spheroid brain. Whether humans have a less flexed basicranium than expected for their relative brain size depends on the phylogenetic vantage point from which it is evaluated. They are as flexed as expected for a descendant of the last common ancestor of the Paranthropus-Homo clade, but their degree of flexion cannot be predicted from the basal hominoid node, even if their relative brain size is specified. Humans undoubtedly occupy an unusual part of morphospace in terms of basicranial flexion and relative brain size, but this does not mean that their degree of flexion is or is not constrained. Curvilinear regression models and standard linear regression models describe the relationship between flexion and relative brain size equally well. Hypotheses that the degree of flexion is or is not constrained cannot be discriminated at present. Consideration of recently published ontogenetic data in the context of the interspecific data for adults suggests that much of the variance in basicranial flexion can still be explained as a mechanical consequence of brain enlargement relative to basicranial length.     

It’s a Lipid’s World: Bioactive Lipid Metabolism and Signaling in Neural Stem Cell Differentiation

Lipids are often considered membrane components whose function is to embed proteins into cell membranes. In the last two decades, studies on brain lipids have unequivocally demonstrated that many lipids have critical cell signaling functions; they are called “bioactive lipids”. Pioneering work in Dr. Robert Ledeen’s laboratory has shown that two bioactive brain sphingolipids, sphingomyelin and the ganglioside GM1 are major signaling lipids in the nuclear envelope. In addition to derivatives of the sphingolipid ceramide, the bioactive lipids discussed here belong to the classes of terpenoids and steroids, eicosanoids, and lysophospholipids. These lipids act mainly through two mechanisms: (1) direct interaction between the bioactive lipid and a specific protein binding partner such as a lipid receptor, protein kinase or phosphatase, ion exchanger, or other cell signaling protein; and (2) formation of lipid microdomains or rafts that regulate the activity of a group of raft-associated cell signaling proteins. In recent years, a third mechanism has emerged, which invokes lipid second messengers as a regulator for the energy and redox balance of differentiating neural stem cells (NSCs). Interestingly, developmental niches such as the stem cell niche for adult NSC differentiation may also be metabolic compartments that respond to a distinct combination of bioactive lipids. The biological function of these lipids as regulators of NSC differentiation will be reviewed and their application in stem cell therapy discussed.