泡果荠属和阴山荠属（十字花科）的分属界限及两属的分类校订

泡果荠属Hilliella和阴山荠属Yinshania是十字花科中国特有的两小群植物,但是,它们的分属界定却一直颇有争议。本文结合前人的研究结果重新对这两群植物做了研究,从而进一步确认这两群植物应该界定为两个属,即泡果荠属和阴山荠属。文中比较了两属间重要的性状特征,并指出它们的主要区别在于:泡果荠属果实无假隔膜,种子较大,表面具小瘤状突起,染色体倍性为六倍体（2n=6x=42）;而阴山荠属果实有假隔膜,种子较小,表面具网纹,染色体倍性为二倍体（2n=2x=12）。两属其他方面如叶形态、叶表皮结构、被毛类型     

阴山荠属的研究

本文为阴山荠属和泡果荠属的分类研究提供了染色体数目试验结果。受试种类按染色体数目的分异形成两大类群,与按形态地理分类法得出的结果一致。阴山荠属(Yinshania):柔毛阴山荠Y.henryi,2n=12;叉毛阴山荠Y.fur-catopilosa,2n=12;乾宁阴山荠Y.qianningensis,2n=12;泡果荠属(Hiliela):昌化泡果荠H.changhuaensis.2n=42,奇异泡果荠H.paradoxa,2n=42;双牌泡果荠H.shuangpaiensis,2n=44;弯缺泡果荠H.sinuata,2n=44;黟县泡果荠H.yixianensis,2n=42。染色体数目结合形态学和地理分布研究,支持两属作为各自独立的属存在。本属是中国特有分布属,形态演化研究表明,本属阴山荠组是较原始的类群,小果组是较进化的类群。文中讨论了属中各种的分布规律和全属的分布特点,认为川西及其邻近地区是本属多样化中心和近代分布区中心,本属可能是从该地区,并随着新生代第三纪喜马拉雅造山运动和青藏高原的崛起,以及第四纪冰期、间冰期的迭次变化产生的全球性气候波动而分化迁移。本属基本上属亚热带、暖温带的半湿润半干旱生态类型。根据本属重要器官较稳定的特点,以及地区性特有成分较高的特点,认为本属是较古老的中国特有成分     

阴山荠属,泡果荠属,棒毛荠属和岩荠属的扫描电镜观察

以阴山荠属、泡果荠属、棒毛荠属和岩荠属的18种植物取用实验材料,对果实外表皮、种子外种皮、毛和花粉粒等特征鉴别器官进行扫描电镜观察,提供36张照片资料,并以检索性状比较属间特征,证明该4属作为各自独立的属是恰当的。     

十字花科一新属——泡果荠属

岩荠属泡果荠组植物(Cochlearia L.Sect.Hilliella O.E.Schulz)不仅在体态上而且在其它一系列特征特别是具泡状突起的果实特征上极不同于狭义的岩荠属(Cohc-learia L.s.str.),松蓝叶岩荠属[Glaucocochlearia(O.E.Schulz)Pobed.],以及拟常绿岩荠属[Pseudosempervium(Boiss)Grossh.]。这些特征看来可以作为建立一新属的根据,因此将其提升为一独立的属并把这一组的8个种组合到新属中去。     

阴山荠属和泡果荠属植物染色体数目及过氧化物酶同工酶的比较

阴山荠属和泡果荠属受试种类的染色体数目是：柔毛阴山荠（Ｙ．ｈｅｎｒｙｉ（Ｏｌｉｖ．）Ｙ．Ｈ．Ｚｈａｎｇ）２ｎ＝１２,叉毛阴山荠（Ｙ．ｆｕｒｃａｔｏｐｉｌｏｓａ（Ｋｕａｎ）Ｙ．Ｈ．Ｚｈａｎｇ）２ｎ＝１２,双牌泡果荠（Ｈ．ｓｈｕａｎｇｐａｉｅｎｓｉｓＺ．Ｙ．Ｌｉ）２ｎ＝４４,黟县泡果齐（Ｈ．ｙｉｃｉａｎｅｎｓｉｓＹ．Ｈ．Ｚｈａｎｇ）２ｎ＝４２,双牌泡果（Ｈ．ｐａｒｄｏｘａ（Ｈａｎｃｅ）Ｙ．Ｈ．Ｚｈａｎ     

关于浙江泡果荠和棒毛荠的分类问题

再次对浙江泡果荠Hilliella warburgii和棒毛荠Cochleariella zhejiangensis作比较研究后,本文认为：两者差别明显,尤其是后者子房和果实表面密被发达的膜质棒状毛,又有各自不同的地理分布区,显然是不同属植物。棒毛荠属(单型属)的属名模式是Cochleariella zhejiangensis Y．H．Zhang et R．Vogt。     

十字花科2个种的染色体数目

棒毛荠 Cochleariella zhejiangensis (Y. H. Zhang) Y. H. Zhang et R. Vogt与浙江泡果荠 H illiella warburgii (O. E. Schulz) Y. H. Zhang et H. W. Li同属十字花科 ,亲缘相近。前者因果瓣具十分独特的棒状毛和其它一些特征而独立成属 [1 ,2 ] 。后者原在广义岩荠属 Cochlearia L.中东亚分布的一个组中 ,该组后经整理扩大提升成泡果荠属 [3]。对于该 2种的分类归属 ,争议颇多。赵一之 [4]认为两者差别不大 ,将前者作为后者的变种更为恰当 ,并将棒毛荠属、泡果荠属与阴山荠属合并。陆莲立 [5]认为两者果瓣上的差别是同一植物种…     

十字花科四属植物叶片脉序的比较研究

对十字花科有争议的岩荠属Cochlearia,泡果荠属Hilliella,阴山荠属Yinshania,棒毛荠属Cochleariella植物的叶片脉序进行了研究。结果表明：岩荠属为环结曲行羽状脉,泡果荠属为直行羽状脉,阴山荠属为半直行羽状脉,棒毛荠属种的叶脉与浙江泡果荠相似。前3属植物在网眼的大小,叶缘末级脉和叶先端的形态上也存在着差异。据此作者认为岩荠属,泡果荠属和阴山荠属这3属植物分开是合理的,但棒毛荠属的分类地位值得进一步研究。还得出这几个属的叶脉演化关系是;基出完全掌状脉→环结曲行羽状脉→直行羽状脉。     

阴山荠属和泡果荠属的数量分类研究

阴山荠属和泡果荠属隶于十字花科,两属有一些共同特征,也有一系列性状差异,数量分类结果支持两属处理为各自独立的属。本文同时对属下类群和一些种间关系进行了讨论。     

人心果和蛋黄果染色体核型分析

研究了山榄科的2种优稀热带果树--人心果及蛋黄果的染色体计数及其染色体形态与核型分析.结果表明:人心果体细胞染色体2n=2X=26,核型类型为2B型,核型公式为18m+8sm,染色体相对长度组成为2L+12M:+8M1+4S,不对称系数为61.71%;蛋黄果体细胞染色体2n=2X=28,核型类型为1A型.核型公式为26m+2sm,染色体相对长度组成为2L+10M2+16M1,不对称系数为59.193%.     

BIOSYSTEMATIC STUDIES IN THE BOUTELOUA CURTIPENDULA COMPLEX. III. POLLEN SIZE AS RELATED TO CHROMOSOME NUMBERS

Pollen size statistics are presented for 10 closely related species of Bouteloua and relationships between pollen size and chromosome numbers are presented for 13 populations of 5 species and 3 varieties. With 1 exception, all populations of all taxa conformed to a general pattern of pollen size dependent upon chromosome number. Chromosome numbers varied from 2n = 20 to 2n = ca. 103, with several independent aneuploid series. Statistical analyses were made of pollen size as related to chromosome number in the 3 varieties of B. curtipendula. These data showed that tetraploids (2n = 40) of var. tenuis had significantly greater pollen size and coefficient of variation than diploids (2n = 20) of the same variety. Similarly, aneuploids of var. curtipendula with 2n = 45 to 2n = 64 chromosomes had significantly larger and more variable pollen than tetraploids (2n = 40) of the same variety. Highly significant positive regression coefficients were obtained from analyses of chromosome numbers and mean pollen size, and chromosome numbers and coefficient of variation, for var. curtipendula. Regression coefficients for var. caespitosa populations with chromosome numbers over the hexaploid (2n = 60) level were not significant.     

广东万年青的细胞遗传学研究

采用常规压片法和去壁低渗法,以生长于广西凭祥和广东深圳的广东万年青（Aglaonema modestumSchott ex Engl.）为材料,对二者的体细胞染色体、花粉母细胞减数分裂染色体配对行为和花粉发育过程进行了观察。结果表明：（1）野生植株为二倍体2n=40,栽培植株为三倍体2n=60;（2）野生种的小孢子母细胞减数分裂前期I终变期均为二价联会,栽培种偶见三价联会;（3）中期I,野生种为20个二价体排列在赤道板上,未见单价体,栽培种的20个二价体排列于赤道板上,20个单价体随机分布于两极,证实其为三倍体;（4）后期I,野生种二价体分离,出现单染色单体桥和断片,几率为10%,栽培种几率为3%,还存在落后染色体,部分不能进入两极的落后染色体和染色体断片在末期I形成微核;（5）四分体时期,野生种未观察到异常孢子,栽培种出现大量败育的四分体或多分体;（6）小孢子进入正常的发育分化,通过两次有丝分裂形成三细胞型花粉。野生种成熟花粉败育率为2.18%,栽培种为88.29%;（7）野生种正常结实,栽培种果实中未发现种子,为高度不育。     

Karyotype morphology and cytogeography in Brunnera and Cynoglottis (Boraginaceae)

A comparative study of karyotype morphology and heterochromatin patterns in Brunnera and Cynoglottis (Boraginaceae) was carried out with traditional methods and Giemsa C-banding. Two polymorphic species of Cynoglottis , each with two subspecies, and two of Brunnera were investigated using native population samples from the central-eastern Mediterranean and Middle East. Pollen size of these samples was measured to investigate relationships with ploidy level. C. barrelieri subsp. barrelieri and subsp. serpentinicola are characterized by In = 18 and smaller pollen grains in contrast to C. chetikiana subsp. chetikiana and subsp. paphlagonica , which are fundamentally tetraploid with 2n = 36. The occurrence of cytotypes with 2n –/2 and 2n = 24 in both subspecies of C. chetikiana , however, would suggest x = 6 as the original haploid number and x = 9 as a derived one. Furthermore, the finding of a hypoploid cytotype with 2n = 16 in C. barrelieri ssp. barrelieri was consistent with previous reports and suggested relationships with Anchusa. Karyoevolutionary processes possibly associated with such a wide chromosome variation in Cynoglottis are discussed. Brunnera macrophylla and B. orientalis share a complement of 2n= 12 and an apparently identical karyotype, which differs from Cynoglottis in terms of asymmetry, chromosome size and morphology. A basic C-banding style was present in Brunnera and Cynoglottis , but heterochromatin content increased from the former to the latter. The parallel increase in chromosome number, heterochromatin content and size of the pollen from Brunnera to Cynoglottis may reflect an evolutionary progression, and is consistent with the supposed ancient origin of Brunnera.     

Karyological studies on Picea ajanensis (Lindl. et Gord.) Fisch. ex Carr. from different regions

Results of karyological study of Picea ajanensis (Lindl. et Gord.) Fisch. ex Carr examined from 13 provenances are presented. In addition to the cytotypes with typical chromosome number (2n = 24), P. ajanensis displays cytotypes with one or two B-chromosomes (2n = 24 + 1 - 2B). Among A-chromosomes, there are 8 pairs of long metacentrics and 4 pairs of shorter meta- or submetacentrics. Among B-chromosomes there are two types of chromosomes: metacentric (B1) and submetacentric (B2) ones. There are many nucleolar chromosomes. Several chromosomes have secondary constrictions. Patterns of B-chromosome distribution within P. ajanensis are have been discussed.     

黄精属的细胞分类学研究——Ⅱ.8个种的核型和进化

作者研究了中国产黄精属8个种的核型,结果如下:热河黄精,2n=22=14m(4SAT)+2sm(SAT)+6st;多花黄精,2n=22=8m+8sm(2sc)+6st(2sc);玉竹,2n=20=12m+8sm;小玉竹,2n=20=8m(2sc)+8sm+4st;长梗黄精,2n=16(18)=10m+4sm+2st;二苞黄精,2n=18=8m+10sm;黄精,2n=24=4m+8sm(2sc)+12st(2sc);卷叶黄精,2n=20=12m(2sc)+8sm。作者推测该属的染色体基数x=10。染色体数目进化的趋势是:非整倍性变异为主,整倍性变异为次;上升性变异为主,下降性变异为次。按照核型不对称程度,8个种的核型可分为三个等级。核型由对称向不对称进化是与染色体数目的进化趋势大体上相关的。     

黄精属的细胞分类学研究Ⅰ.8个种的核型和进化

作者研究了中国产黄精属8个种的核型,结果如下:热河黄精,2n=22=14m(4SAT)+2sm(SAT)+6st;多花黄精,2n=22=8m+8sm(2sc)+6st(2sc);玉竹,2n=20=12m+8sm;小玉竹,2n=20=8m(2sc)+8sm+4st;长梗黄精,2n=16(18)=10m+4sm+2st;二苞黄精,2n=18=8m+10sm;黄精,2n=24=4m+8sm(2sc)+12st(2sc);卷叶黄精,2n=20=12m(2sc)+8sm。作者推测该属的染色体基数x=10。染色体数目进化的趋势是:非整倍性变异为主,整倍性变异为次;上升性变异为主,下降性变异为次。按照核型不对称程度,8个种的核型可分为三个等级。核型由对称向不对称进化是与染色体数目的进化趋势大体上相关的。     

Karyotype Analysis of Eight Species of Polygonatum Mill.

Meiosis and mitosis of eight species of Polygonatum from Sichuan, China, were investigated. Ten bivalents (n=10) at meiosis of pollen mother cells were observed ih P. odoratum (Mill.) Druce. Meiotic observation on pollen mother cells in P. punctatum Royle ex Kunth shows 16 bivalents (n=16) at diakinesis, P. kingianum Coll. et Hemsl. 13 bivalents (n=13) at diakinesis. P. zanlanscianense Pamp. 15 bivalents (n=15) in MI, P. cirrhifolium (Wall.) Royle 28 bivalents at diakinesis. Somatic chromosomes were observed in root tip cells. The karyotype formulae are as follows: P. odoratum (Mill.) Druce K(2n)=20=4st+6sm+10m; P. cyrtonema Hua K(2n)=20=6sm+14m; P. punctatum Royle ex Kunth K(2n)=32=2t+8st+ 2sm+20m; P. kingianum Coll. et Hemsl. K(2n)=26=8st(2SAT)+14sm+4m; P. alternicirrhosum Hand.-Mzt. K(2n)=32=6st+8sm+18m(2SAT); P. zanlanscianense Pamp. K(2n)=30= 2t+6st+6sm+16m(2SAT); P. sibiricum Delar. ex Redouté K(2n)=24=2t+14st(2SAT)+6sm +2m; P. cirrhifolium (Wall.) Royle K(2n)=56=18st+10sm+28m. The karyotypes of P. punctatum, P. kingianum, P. alternicirrhosum and P. cirrhifolium are reported for the first time. Both the chromosome number and structure in our materials are certainly different from thoses in previous reports. Of numerical variation polyploidy and aneuploidy have been recorded, and aneuploidy is frequent. According to degree of asymmetry of the karyotype, the 8 species are divided into three types: 2B, 3B, 2C. The increasing asymmetry is correlated with the increasing of the chromosome number. There are no obvious differences in chromosome number and karyotype between Alternifolia Baker and Verticillate Baker. They are not two natural groups. This opinion is different from that of Therman and Suomalainen.     

国产毛茛属植物五种一变种的核型

对国产6种毛茛属(Ranunculus)植物进行了核型研究。它们的核型公式分别为:扇叶毛茛2n=4x=32=12m 20sm,云生毛茛2n=4x=32=16m 10sm 6st,曲升毛茛2n=4x=32=14m 16sm 2st及2n=5x=40=18m 16sm 4st 2T,西南毛茛2n=2x=16=12m 2sm 2st,匍枝毛茛2n=2x=16=8m 8st及2n=4x=32=12m 4sm 14st 2t,棱喙毛茛2n=2x=16=6m 6sm 4st。并结合形态及孢粉的资料就毛茛属植物核型不对称系数对分类的意义进行分析。     

加拿大披碱草和圆柱披碱草BC1植株的细胞遗传学鉴定

以加拿大披碱草(2n=4x=28)和圆柱披碱草(2n=6x=42)为材料,对其BC1植株染色体数目、配对构型以及花粉育性和结实性等进行了鉴定。结果显示:BC1代85%以上细胞染色体数目为28条(2n=4x=28);BC1植株的花粉母细胞减数分裂中期Ⅰ染色体配对行为较规则,其平均染色体构型分别为0.04Ⅰ 13.98Ⅱ,且环状二价体多于棒状二价体;BC1植株的花粉可育率和自然结实率分别为84.83%和70.98%,说明BC1植株的育性已得到恢复,为其后代优良株系的选育奠定了基础。     

东北地区野豌豆属Vicia L.物种生物学研究——Ⅰ.染色体数目及其分类学意义

本文首次全面地报道了东北地区野豌豆属植物染色体数目。结果如下:Vicia cracca group. 2n=12; V. amurensis Oett. 2n=12; V. amocna Fisch. ex DC. 2n=24; V. amocna var. oblongifolia Reqel 2n=12; V. amoena var. scricca Kitag. 2n=12; V. geminiflora Trautv. 2n=14; V. japonica A. Gray 2a=24; V. pseudorobus Fisch, et C. A. Mey 2n=12; V. multicauhs Ledeb. 2n=12; V. venosa (Willd.) Maxim, 2n-12; V. ramuliflora (Maxim.) Ohwi 2n=12, 24; V. unijuga A. Br. 2n=12, 24.结果表明该地区野豌豆种类以x=6为主,并以该基數形成较高频率的多倍体类群。根据染色体资料,对于长期存在争议的种下处理或近緣种类的划分,作者提出了新的见解。