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以添加1.5%活性碳及3%的蔗糖的花宝1号和MS培养基为基础,加入不同浓度的BA和IBA,用外植体诱导的第1代组培苗基部以下连根1.1~1.2 cm为材料诱导原球茎.结果表明,花宝1号培养基优于MS培养基,BA1 mg/L IBA0.1 mg/L最合适,诱导率可达90.1%;光照以10 h/d左右为宜,培养温度以25~27℃最佳,其诱导率最高,生长状况最好. 相似文献
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兰属小金童(Cymbidium Golden elf. 'Sundust')茎尖组织培养研究表明:茎尖外植体在不添加植物生长调节剂的花宝1号培养基上诱导产生原球茎;原球茎在花宝1号3g/L + 6-BA 1.5mg/L的液体培养基上培养,能形成快速增殖的丛生原球茎;不同的培养方式对原球茎的增殖效果有显著差异;原球茎在不附加植物生长调节剂的1/2MS或花宝1号培养基上分化成无根小苗,然后在花宝1号+NAA 0.2mg/L+IBA 0.6mg/L培养基上诱导生根,进而再形成完整植株. 相似文献
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兰属小金童(Cymbidium Golden elf.‘Sundust’)茎尖组织培养研究表明:茎尖外植体在不添加植物生长调节剂的花宝1号培养基上诱导产生原球茎;原球茎在花宝1号3g/L 6-BA 1.5mg/L的液体培养基上培养,能形成快速增殖的丛生原球茎;不同的培养方式对原球茎的增殖效果有显著差异;原球茎在不附加植物生长调节剂的1/2MS或花宝1号培养基上分化成无根小苗,然后在花宝1号 NAA 0.2mg/L IBA 0.6mg/L培养基上诱导生根,进而再形成完整植株。 相似文献
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火焰兰杂交种的胚培养和离体快繁 总被引:1,自引:0,他引:1
1植物名称火焰兰杂交种(Renanthera CoCCinea×R.imschootiana). 2材料类别种子. 3培养条件种子萌发培养基:(1)VW;(2)VW 椰子乳100mL·L-1;(3)KC;(4)KC 椰子乳100mL·L-1;(5)VW 椰子乳100mL·L-1 活性炭2 g·L-1(6)1/2MS 椰子乳100 mL·L-1.叶片离体培养基:(7)VW 2,4-D 1.0 mg·L-1(单位下同) 6-BA 2.5 NAA0.2;(8)VW 2,4-D 2.0 6-BA 5.0 NAA 0.5.原球茎继代增殖:(9)花宝1号1.5 g·L-1 花宝2号1.5g·L-1 椰子乳100 mL·L1 6-BA 1.0 NAA 1.0;(10)VW 椰子乳100mL·L-1 6-BA 1.0 NAA 1.0.生根壮苗培养基:(11)花宝1号3 g·L-1 蛋白胨2 g·L-1 活性炭2 g·L-1 NAA 0.5 6-BA 0.2;(12)花宝1号1 g·L-1 花宝2号1 g·L-1 蛋白胨2 g·L-1 活性炭2g·L-1 NAA 0.5 6-BA 0.2.以上培养基均加1.5%蔗糖、0.6%琼脂,pH 5.2~5.4,培养温度(25±2)℃,光照度1 500~2000 lx,光照时间12 h·d-1. 相似文献
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以文心兰切花品种‘南茜’原球茎(PLB)及试管苗为材料,在培养基MS+6-BA 2 mg/L+Ad 1 mg/L+NAA 0.1 mg/L+蔗糖30 g/L基础上,利用增殖系数及不同形态指标加权分析研究马铃薯、芋头、南瓜、香蕉及活性炭(AC)5种培养基添加物及其组合对‘南茜’PLB增殖及试管苗生长的影响。结果表明,适宜文心兰PLB增殖培养的添加物为马铃薯泥50 g/L,培养45 d后,PLB增殖系数是对照的3.64倍;适宜无菌小苗(约1.5 cm)增殖及生长的添加物为香蕉泥50 g/L+AC 1 g/L,加权总分是对照的1.53 倍;适宜无菌中苗(约2.5 cm)增殖及生长的添加物为马铃薯泥25 g/L+香蕉泥25 g/L,加权总分是对照的1.55倍。 相似文献
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1 植物名称多花脆兰[Acampe rigida(Buch.-Ham.ex J.E.Smith)P.F.Hunt].
2 材料类别种子.
3 培养条件种子萌发培养基:(1)MS;(2)1/2MS(大量元素用量减半);(3)KC[成份如下:MgSO4·7H2O 250 mg·L-1(单位下同);KH2PO425O;(NH4)2SO4 500; Ca(NO 3)2·2H2O 1 000 ;FeSO4·7H2O 25; MnSO4.4H2O 7.5;肌醇(C6H12O6)100;盐酸硫胺素(VB,)1; D-甘露糖醇12.7%];(4)1 g·L-1花宝1号(美国Haponex公司产品,N:P:K=7:16:9)+2 g.L-1花宝2号(美国Haponex公司产品,N:P:K=20:20:20);(5)MS+100 g·L-1香蕉汁;(6)1/2MS+100 g·L-1香蕉汁;(7)KC+100 g·L-1香蕉汁;(8)1 g·L-1花宝1号+2 g·L-1花宝2号+100g·L-1香蕉汁.原球茎增殖和分化成苗培养基:(9)1/2MS+6.BA 1.5+NAA 0.2+100 g·L-1香蕉汁;(10)1/2MS+6-BA 0.5+NAA 0.05+100 g·L-1香蕉汁;(11)1 g·L-1花宝1号+2 g·L-1花宝2号+6-BA 1.0+NAA0.5+100 g·L-1香蕉汁. 相似文献
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以春兰茎尖、腋芽为外植体,接种在1/2MS 6-BA 5.0mg/L NAA 0.5mg/L培养基上形成根状茎,根状茎在1/2MS 6-BA 0.2mg/L NAA 2.0mg/L 香蕉泥100g/L培养基上可大量增殖,并伸长生长形成丛生型根状茎;根状茎在MS 6-BA 2.0mg/L NAA 1.0 mg/L培养基上可分化成小苗,分化率达30.6%;小苗在1/2MS IBA 2.0mg/L 香蕉泥150g/L培养基上可生根壮苗,生根率达100%。 相似文献
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Svinningen AE Rashani KP Jegathambigai V Karunaratne MD Mikunthan G 《Communications in agricultural and applied biological sciences》2010,75(3):391-397
Queen palm, Livistona rotundifolia foliage contributes greatly in export industry. Red spider mite (RSM) (Tetranychus urticae) infests on the foliage and reduces its affordable market quality. T. urticae is found in dry environment and is one of the phytophagous mite belongs to family Tetranychidae. Different chemicals such as 80% sulphur + Diazinon @ (50g+12ml/10L) are recommended against red spider mite, but these have lesser effect on this tiny mite. Since these chemicals are not environment friendly, Green Farms Ltd., in Sri Lanka prefers to use biological agents for mite management. Extracts of Curcuma aeruginosa rhizome and Adhatoda vasica plant parts were studied separately causing mortality on T. urticae. Field experiments were conducted to study the efficacy of C. aruginosa extract for controlling RSM on L. rotundifolia leaves. Curcuma aruginosa was tested at concentrations of 2, 5, 10, 15, 20 and 25 g/L and a control with equal amount of water. C aruginosa extracts of different concentrations were treated six times at five days interval on the palms separately. Living spider mites and eggs were pre-counted in marked leaves before applying C. aruginosa extracts. Next count was taken a day prior to next spraying. The result revealed that all the concentrations except 2 g/L were found to be effective compared to control. However there was no difference between the concentrations from 5 to 25 g/L. Hence C. aruginosa rhizome extract at its lowest concentration of 5 g/L is equally effective for the control of RSM on L. rotundifolia leaves. In another experiment extracts of Adhatoda vasica bark, leaves, and flower and water as control were applied thrice with three days interval. Pre treatment counting of living spider mites and eggs were taken in marked leaves. Post count was taken a day prior to next spraying. Third and forth counting were done after three days and four weeks from final spraying respectively. The results revealed that bark, flowers were found to be more effective compared to control. Flowers and bark were the best and hence there is no need of third sprayings as almost all the spider mites population were eradicated after second spraying. Flower extraction showed best performance until three months since final spraying. Flower and bark extracts showed higher acaricidal property and leaf showed moderate acaricidal property. 相似文献
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The production of strawberry plants from callus cultures 总被引:1,自引:0,他引:1
Shoots were regenerated from callus of the commercially important strawberry varieties Bogota, Brighton, Cambridge Favourite, Hapil, Ostara, Rapella, Red Gauntlet and JILA33 which is a promising selection from a current breeding programme.The callus was initiated from explants of petiole or lamina of leaves of micropropagated shoots in vitro or of lamina or peduncle from greenhouse plants. There was more shoot regeneration with callus from lamina than from petiole although with the variety Hapil, regeneration occurred only with callus from peduncle.With seven of the varieties, shoot regeneration occurred on culture media with BAP and 2,4-D whilst with the remaining variety, Cambridge Favourite, it occurred only with medium which contained 1AA- alanine conjugate in place of 2,4-D.Regenerated shoots rooted readily and the plants produced are being studied for somaclonal variation. 相似文献
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以‘红芭拉蒂’、 ‘夏黑’、 ‘户太8号’3个葡萄品种为材料,研究叶面喷施有机硒肥对温室葡萄品质和硒含量的影响。结果表明: 在果实幼果期和膨大期,叶面喷施12 mg·L-1氨基酸螯合态富硒叶面肥不仅能使葡萄果实产量和硒含量显著提高,还能显著提高各品种葡萄果实综合品质(可溶性糖、有机酸、可溶性蛋白质、可溶性固形物、Vc和原花青素等),但白藜芦醇未显著提高。各品种中,2017和2018年夏黑的硒含量较对照分别增加36.7%和37.1%,其增幅高于红芭拉蒂和户太8号;施硒组红芭拉蒂以高糖低酸的口感及高保健成分在品质综合比较中表现较优;2018年施硒的户太8号硒含量为53.26 μg·kg-1,显著高于其他品种,硒富集能力较强。因此,夏黑硒含量增幅较大;红芭拉蒂在营养价值和品质上表现较优;户太8号适合富硒葡萄生产。 相似文献
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采用硫酸-苯酚法、AlCl3比色法、酸性染料比色法测定铁皮石斛(Dendrobium officinale)花、叶、茎中多糖、黄酮、生物碱含量,通过DPPH和ABTS清除实验评价铁皮石斛花、叶、茎的水提物和乙醇提取物的体外抗氧化活性。结果表明,铁皮石斛不同部位的多糖含量茎>花>叶,黄酮含量花>叶>茎,生物碱在各个部位分布均较少。其中茎的多糖含量可达23.92%,花中黄酮含量可达1.847%。抗氧化能力评价表明,铁皮石斛花水提物、茎醇提物、花醇提物的DPPH自由基清除能力相对较好,半效应浓度(EC50)分别为410.4 μg·mL-1、454.1 μg·mL-1、573.2 μg·mL-1;铁皮石斛茎醇提物、花醇提物、花水提物ABTS自由基清除能力相对较好,半效应浓度(EC50)分别为61.1 μg·mL-1、62.2 μg·mL-1、103.0 μg·mL-1。铁皮石斛花的提取物抗氧化活性整体优于叶和茎,醇提物抗氧化能力优于水提物。 相似文献
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柑橘体细胞杂种砧木对脐橙幼树生长及根和叶中抗氧化酶系活性的影响 总被引:4,自引:0,他引:4
以体细胞杂种红橘 枳、红橘 粗柠檬和有性杂种Troyer枳橙、Swingle枳柚作砧木的脐橙二年生嫁接苗为试材,于盆栽条件下研究砧木对苗木生长及根和叶中抗氧化酶系活性影响的结果表明,红橘 枳的生长势和花量明显强于和大于其它砧木,红橘 粗柠檬的生长和花量表现介于两种有性杂种之间;砧木不影响叶片中可溶性蛋白含量和超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性年动态变化特征.4、6、8月,叶中POD活性高低分别与各次新梢生长量和树冠体积(8月)大小呈负相关;12月,根系与叶片的SOD和POD活性分别呈正相关,根系可溶性蛋白含量和根系CAT活性呈正相关. 相似文献
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