Evolution of temperature and salt structure of Lake Bonney, a chemically stratified Antarctic lake

A resurgence of interest in the ecology of perennially ice-covered lakes in the McMurdo dry valleys has necessitated a review of our knowledge of the physical and chemical properties of these unusual lakes. Salinities in the ice-covered lakes cover a range from freshwater to hypersaline brines. Recent measurements of salt composition and concentrations in Lake Bonney reveal little change below the chemocline since extensive measurements made in 1960–1961, although lake level has risen by approximately 5 m since that time. The rise in lake level has resulted in a thickening of the freshwater layer above the chemocline. Temperature structure has adjusted to the effects of increased lake level on heat transfer processes such as transmission and absorption of solar radiation in the water column.Questions about how water-column stability affects biology in Lake Bonney have motivated the formulation of a method to compute density from in situ measurements of temperature, conductivity and pressure. Owing to high salt concentration and unique ion ratios, we modified the UNESCO Equation of State for seawater to predict density at salinities greater than 42. The modifications merge smoothly with the UNESCO equations at a salinity of 42. At salinities below 42 the UNESCO equations give excellent predictions of density.     

Species succession and the development of a lacustrine fish community in an ephemeral lake

Here, we present a gillnet survey of Lake Liambezi a 370 km² shallow ephemeral floodplain lake situated in north-eastern Namibia, which is fed irregularly by the upper Zambezi and Kwando Rivers during years of high flooding. The lake dried up in 1985 and, with the exception of sporadic minor annual inundation events, remained dry until 2007. We describe the temporal succession of fish species over an 8 year period from initial inundation 2007 to maturation in 2014. The succession of the fish community did not follow the typical pattern of opportunistic strategists during colonisation, to periodic strategists that are eventually succeeded by equilibrium strategists. Instead, the evolution of the fish community was characterised by three distinct phases. The first phase involved the inundation and colonisation of the lake in 2007, followed by its decline until the floods that filled the lake in 2009. During this phase the lake was colonised by fishes from the adjacent upper Zambezi and Chobe River floodplains. Fish communities predominantly comprised floodplain specialists including the barbs Enteromius paludinosus and Enteromius poechii, the mormyrid Marcusenius altisambesi and catfishes Schilbe intermedius and Clarias gariepinus. The filling of the lake in the March 2009 floods marked the beginning of the second, successional phase. The barbs declined in abundance and the alestid Rhabdalestes maunensis underwent explosive population growth between 2009 and 2010, but populations crashed equally rapidly and were replaced by Brycinus lateralis which, together with S. intermedius went on to dominate the fish community 2011–2014. Larger, slower growing tilapiine cichlids increased steadily in abundance and became the dominant components in a 2700 t y^–1 artisanal fishery that developed on the lake. The fish community in the ephemeral Lake Liambezi is clearly influenced by numerous factors including connectivity, lake level fluctuations, competition and the effects of fishing, which may disrupt typical succession processes in floodplain ecosystems.     

氮、磷对热带浅水湖泊惠州西湖蓝藻的控制

湖泊富营养化常导致蓝藻生物量的增加,水质恶化.于2011年2月至12月对热带浅水湖泊惠州西湖六个湖区的蓝藻群落结构进行研究,以了解其时空变化特征及主要影响因素.结果表明,以沉水植物为优势的元妙观湖区与南南湖蓝藻无明显的优势种;平湖蓝藻优势种为银灰平裂藻(Merismopedia glauca)和湖丝藻(Limnothrix sp.),南丰湖、北丰湖和北南湖的主要优势种均为银灰平裂藻(Merismopedia glauca).平湖、南丰湖、北丰湖和北南湖蓝藻丰度及生物量存在显著的季节变化.相关分析显示惠州西湖夏季蓝藻生物量受氮、磷盐控制.冬季温度的影响,蓝藻生物量与氮、磷的相关性不显著.     

Seasonal and Interannual changes of chemical characteristics and phytoplankton in a mountain lake of the Eastern Italian Alps (Lake Calaita,Trentino)

Measurements at Lake, Calaita, a small mountain lake located at 1605 m. a.s .l. in a metamorphic catchment area in the south of the Trentino Region (Northern Italy), have revealed great seasonal variations in the chemical characteristics and phytoplankton community during the ice free period in 1992 and 1993. The acidity present in wet precipitations (H⁺, NH₄⁺) was neutralised within the drainage basin by mineral dissolution which led to an increase of basic cations and alkalinity in the runoff. The dilution during periods of higher discharge, e.g. in spring and autumn, resulted in low values of alkalinity (up to 60 μeq 1⁻¹), pH (mostly <6.7) and conductivity (<8 μS cm⁻¹, 20°C). In summer, the decrease in runoff caused higher alkalinity (>10 μeq 1⁻¹), pH (6.9–7.4) and conductivity values (up to 30 μS cm⁻¹). The phytoplankton showed a major development in summer (with biovolume values of up to 7000 mm³ m⁻³), two different taxa being dominant in 1992 (Oocystis cf. lacustris) and 1993 (Synedra sp.). Unpredictable climatic conditions have a strong influence on the physical stability of the lake, which makes it more difficult to explain the evolution of the phytoplankton community as opposed to deeper lakes.     

Seasonal changes in the chemistry and biology of a meromictic lake (Big Soda Lake,Nevada, U.S.A.)

Big Soda Lake is an alkaline, saline lake with a permanent chemocline at 34.5 m and a mixolimnion that undergoes seasonal changes in temperature structure. During the period of thermal stratification, from summer through fall, the epilimnion has low concentrations of dissolved inorganic nutrients (N, Si) and CH₄, and low biomass of phytoplankton (chlorophyll a ca. 1 mgm ^-3). Dissolved oxygen disappears near the compensation depth for algal photosynthesis (ca. 20 m). Surface water is transparent so that light is present in the anoxic hypolimnion, and a dense plate of purple sulfur photosynthetic bacteria (Ectothiorhodospira vacuolata) is present just below 20 m (Bchl a ca. 200 mgm^-3). Concentrations of N H₄ ⁺, Si, and CH₄ are higher in the hypolimnion than in the epilimnion. As the mixolimnion becomes isothermal in winter, oxygen is mixed down to 28 m. Nutrients (NH₄ ⁺, Si) and CH₄ are released from the hypolimnion and mix to the surface, and a diatom bloom develops in the upper 20 m (chlorophyll a > 40 mgm^-3). The deeper mixing of oxygen and enhanced light attenuation by phytoplankton uncouple the anoxic zone and photic zone, and the plate of photosynthetic bacteria disappears (Bchl a ca.10mgm^-3). Hence, seasonal changes in temperature distribution and mixing create conditions such that the primary producer community is alternately dominated by phytoplankton and photosynthetic bacteria: the phytoplankton may be nutrient-limited during periods of stratification and the photosynthetic bacteria are light-limited during periods of mixing.     

The influence of the diel climatic cycle on the depth-time distribution of phytoplankton and photosynthesis in a shallow equatorial lake (Lake Baringo,Kenya)

Lake Baringo is a shallow equatorial lake. This paper reports a diel study of the depth-time distribution of phytoplankton and photosynthesis at one location in Lake Baringo on 10 March 1989. The water column shows a pattern of diurnal stratification probably accentuated by the high turbidity of the water and therefore rapid attenuation of solar energy. This stratified pattern breaks down at night due to atmospheric cooling and the regular onset of winds in the early evening. The phytoplankton is dominated byMicrocystis aeruginosa with some associated epiphytes. It concentrates in the narrow euphotic zone during the diurnal period of stratification due to buoyancy of theMicrocystis; evening breakdown of the thermocline results in the phytoplankton being mixed throughout the water column. A series of measurements of photosynthesis throughout the diurnal period gives an areal rate of 3.8 g O₂ m⁻² d⁻¹. The relationship between this value and the level of fish exploitation in Lake Baringo is discussed. The diel cycle in Lake Baringo is interpreted as dominating over any seasonal limnological cycle in the lake.     

Punctuated equilibria, morphological stasis and the palaeontological documentation of speciation: a biological appraisal of a case history in an African lake

The present-day faunas of the great African lakes present some of the world's best examples of 'explosive speciation'. Lakes Victoria and Malawi each probably have several hundred endemic species of cichlid fishes. Much can be inferred about the evolution of these fishes from morphology, behaviour and intra-lacustrine distribution and from the fact that they include taxa ranging from local races, through sibling species, to forms that display extensive differentiation. The time taken to acquire specific distinctness can sometimes be accurately defined, but fossil lineages are unknown. A recent study of a fossil sequence of molluscs in the Turkana basin throws new light on the history of African lake faunas. It also claims to have resolved events during speciation. While critical analysis based on our knowledge of living molluscs in this area fails to substantiate this claim, the fossil molluscs complement information provided by the biology of extant fishes and invertebrates and emphasize the importance of these lakes in the study of evolution in living and extinct populations.     

<Emphasis Type="Italic">Thiocapsa imhoffii</Emphasis>, sp. nov., an alkaliphilic purple sulfur bacterium of the family <Emphasis Type="Italic">Chromatiaceae</Emphasis> from Soap Lake,Washington (USA)

An alkaliphilic purple sulfur bacterium, strain SC5, was isolated from Soap Lake, a soda lake located in east central Washington state (USA). Cells of strain SC5 were gram-negative, non-motile, and non-gas vesiculate cocci, often observed in pairs or tetrads. In the presence of sulfide, elemental sulfur was deposited internally. Liquid cultures were pink to rose red in color. Cells contained bacteriochlorophyll a and spirilloxanthin as major photosynthetic pigments. Internal photosynthetic membranes were of the vesicular type. Optimal growth of strain SC5 occurred in the absence of NaCl (range 0–4%), pH 8.5 (range pH 7.5–9.5), and 32°C. Photoheterotrophic growth occurred in the presence of sulfide or thiosulfate with only a limited number of organic carbon sources. Growth factors were not required, and cells could fix N₂. Dark, microaerobic growth occurred in the presence of both an organic carbon source and thiosulfate. Sulfide and thiosulfate served as electron donors for photoautotrophy, which required elevated levels of CO₂. Phylogenetic analysis placed strain SC5 basal to the clade of the genus Thiocapsa in the family Chromatiaceae with a 96.7% sequence similarity to its closest relative, Thiocapsa roseopersicina strain 1711^T (DSM217^T). The unique assemblage of physiological and phylogenetic properties of strain SC5 defines it as a new species of the genus Thiocapsa, and we describe strain SC5 herein as Tca. imhoffii, sp. nov.     

Factors affecting the seasonality and distribution of cyanobacteria and chlorophytes: a case study from the large lakes south of the Alps,with special reference to Lake Garda

Chlorophytes and cyanobacteria are among the most typical algal groups, contributing to the aesthetic appearance and quality of the epilimnetic waters of the deep (251–410 m) and large (6.5–49×10⁹ m³) lakes located on the southern edge of the Alps (from oligo-mesotrophy to meso-eutrophy: Maggiore, Garda, Como, Iseo and Lugano). The results obtained from monthly surveys carried out in the largest of these lakes (Garda) have been reported in detail. The thermal stability of the water column and silica depletion were the main factors responsible for the decline of the great spring diatoms. The successive growth of Mougeotia sp. was favoured by its lower sinking velocity and resistance to the increasing grazing pressure. During summer, the maximum stability of the water column, with high vertical nutrient concentration gradients, determined a major algal differentiation with a typical increase, among others, of Chlorococcales at the surface and metalimnetic stratification of various Oscillatoriales. The development of oligotrophic blooms, caused by a rapid thickening at the surface of Anabaena in the eastern, sheltered basin, was also discussed in light of the trophic characterisation of Lake Garda. From autumn to spring, the decreasing light, the increasing mixing depth and nutrient availability favoured a progressive dominance of vertical homogeneous populations of Planktothrix (autumn) and colonial diatoms. The same functional groups of chlorophytes and cyanobacteria have been recognised in the studies published so far on the phytoplankton of deep southern subalpine lakes. In this respect, their morphometric and physical properties appear to constitute a sort of standardising factor, reducing the range of possible dominants. With increasing TP concentrations and biomass, filaments of Planktothrix and Planktolyngbya, along with the Chlorococcales, became important. The dominance of Mougeotia (one of the most characteristic features of these large lakes) appears restricted to a medium trophic range, whereas the distribution of the Chroococcales and other filaments ascribed to Pseudanabaena and/or Limnothrix is more irregular. The conspicuous presence of Aphanizomenon in Lake Lugano is typical and characteristic of a more productive lake. Among the Nostocales, a clear interpretation of the Anabaena blooms in lakes Garda and Iseo is complicated by the peculiar behaviour of filaments concentrating at the surface, which is apparently restricted, within the medium trophic range, to stable water columns.     

Analysis of the genomic organisation of a small chromosome of Leishmania braziliensis M2903 reveals two genes encoding GTP-binding proteins, one of which belongs to a new G-protein family and is an antigen

Leishmania braziliensis M2903 contains a highly amplified small chromosome. This work is aimed at resolving its structural organization and determining whether this unusual chromosome contains specific genes encoding proteins with important functions in disease pathology or drug resistance. Our results show that the M2903 250-kb small chromosome contains LD1 sequences and has an inverted repeat structure. The LD1 sequences and two cDNAs (cDNA2 and cDNA53) were mapped on a cosmid contig, and the two cDNAs and the corresponding genomic fragments from the small chromosome were sequenced. The gene encoding cDNA2 predicts a putative GTP-binding protein with homology to other GTP-binding proteins only in the G-1 domain region; however, four other conserved motifs can be recognized. Sequence similarity to cDNA53 is located in at least five chromosomes, and its small chromosome copy is a pseudogene. An open reading frame downstream of the cDNA53 pseudogene predicts another GTP-binding protein that belongs to a new G-protein family with an unusual conserved GTP-binding domain and a newly characterized conserved sequence motif. A portion of this GTP-binding protein gene was studied previously in L. aethiopica as a recombinant antigen that reacts with human antibodies.     

Expression of recombinant capsid proteins of chitta virus, a genogroup II Norwalk virus, and development of an ELISA to detect the viral antigen

The second open reading frame (ORF2) gene of the Chitta virus (CHV) was cloned to construct a recombinant baculovirus. The CHV ORF2 is predicted to encode a capsid protein of 535 amino acids (aa). CHV showed a high aa identity in the capsid region with genogroup II Norwalk virus (NV) (65-85%), but a low aa identity with genogroup I NV (44-46%). Phylogenetic analysis of the ORF2 gene demonstrated that CHV is genetically closely related to the Hawaii virus included in genogroup II NV. The recombinant capsid protein of CHV (rCHV) self-assembled to form empty virus-like particles (VLPs) when expressed in insect cells with the recombinant baculovirus. An enzyme-linked immunosorbent assay (ELISA) based on antisera to rCHV was developed to detect CHV antigen in stools. The antigen ELISA appeared to be highly specific to both rCHV and CHV-like strains. In addition, combined use of antigen ELISAs using antibodies against two antigenically distinct recombinant VLPs, the recombinant Chiba virus (rCV) and recombinant Seto virus (rSEV), enabled us to determine the genetic as well as antigenic relationship among these three viruses.