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1.
As compared to doubled haploid plants of the same origin, haploid tobacco plants are characterized by narrow leaves and in these leaves the endogenous concentration of gibberellins was considerably higher than in doubled haploids. This higher GA activity is almost entirely due to elevated levels of polar gibberellins. The same leaf shape as in haploids could be induced by GA3 sprays to doubled haploids. A similar leaf shape was also observed on tissue culture derived so called NICA plants displaying the morphology of tobacco plants as described by Dudits et al. (1987) from whom the plant material was obtained as a gift. Here, in the leaves of a special strain with narrow lamina again a much higher gibberellin activity was detected than in the leaves of plants of the original tobacco strain. Histochemical determination of the relative DNA content indicated that leaves of NICA were chimaeras containing 1C cells besides cells with higher C values. Obviously, haploidy is somehow related to the endogenous gibberellin activity in tobacco plant material with consequences on the morphological appearance of 1n plants. Comparing some haploid and doubled haploid strains in tissue culture and pot and field experiments in several years apparently the genotype of the plant material is more significant for nicotine concentration than the ploidy level.Abbreviations DW dry weight - FW fresh weight - LSI leaf shape index  相似文献   

2.
Monitoring of banana streak badnavirus (BSV) antigens and symptoms in naturally BSV-infected plantain and banana (Musa spp.) plants showed a great variation in symptom expression, distribution and relative concentration of BSV between and within plants. Expression and distribution of symptoms was erratic within individual leaves as well as between different leaves of the same plant. The concentration of BSV antigens detected by triple antibody sandwich enzyme linked immunosorbent assay (TAS-ELISA) varied in different plant parts including leaf lamina, midrib and pseudostem, roots and young ‘cigar’ leaf. The concentration of BSV antigens was high in symptomatic tissues but was low or below the limits of detection in most asymptomatic tissues. During ‘hot dry’ seasons when symptoms were not fully expressed, the concentration of BSV antigens in leaf tissues declined drastically, often below the detection limit of TAS-ELISA. These results suggested that for more reliable detection of BSV antigens by TAS-ELISA, it is advisable to index plants using composite tissue samples comprising as many leaves as possible for each plant and collected during cool and/or rainy seasons when symptom expression is generally severe.  相似文献   

3.
Yukio Kato  Shigeru Kawahara 《Planta》1972,107(2):111-120
Summary Isolated leaves, leaf fragments and pieces of the midrib portion devoid of lamina, of Heloniopsis orientalis were grown on an inorganic nutrient medium without organic nutrients and growth regulators in order to investigate their regenerative ability. Bud formation in intact, attached leaves occurs only at the tip, in isolated leaves at the tip and the base, whereas leaf fragments cut transversely at a distance from the tip and isolated midrib pieces form numerous shoot buds in a random distribution. Lamina fragments lacking midrib frequently fail to regenerate even after a long time of culture. It is suggested that endogeneous growth regulators in the leaf, especially the vascular tissues, play an important role in bud initiation. Very young leaves of Heloniopsis are capable forming buds and roots when isolated from the mother plants.  相似文献   

4.
Two-year-old Navel orange scions (Citrus sinensis (L.) Osbeck) budded to either Cleopatra mandarin (C. reticulata) and Troyer citrange (C. sinensis × P. trifoliata) rootstocks were used in this experiment. Cleopatra manda in rootstock was considered more tolerant to salinity than Troyer citrange, and this property was attributed to a greater capacity to exclude chloride ions.Plants were grown under glasshouse conditions and supplied with nutrient solution containing either no or 45 mM NaCl. Calcium concentration was increased from 3 to 30 mM. Sodium, potassium, calcium and chloride concentrations in plant organs were analyzed after 90 days of treatment.Supplemental Ca was found to mitigate the adverse effects of salinity on plant growth, defoliation or leaf injury.Chemical analysis indicated that in plants grafted on Troyer citrange Ca restricted uptake and subsequent translocation of Na to the leaves and increased K concentration in both roots and leaves. However, in Cleopatra mandarin-grafted plants increasing Ca levels seemed to reduce transport of Na from roots to leaves, and Na accumulation in roots was associated with reduced concentration of K in this rootstock.Organ chloride analysis showed that Cl accumulation in leaves of plants grafted on both rootstocks was reduced when external Ca concentration increased, whereas Cl concentration in roots remained constant or increased. The data of distribution of Cl in plants showed that a high external Ca level increased Cl accumulation in the basal stem and roots, and reduced the transport of Cl from roots to leaves.  相似文献   

5.
R. S. Poethig  I. M. Sussex 《Planta》1985,165(2):170-184
The cellular parameters of leaf development in tobacco (Nicotiana tabacum L.) have been characterized using clonal analysis, an approach that provides unequivocal evidence of cell lineage. Our results indicate that the tobacco leaf arises from a group of around 100 cells in the shoot apical meristem. Each of these cells contributes to a unique longitudinal section of the axis and transverse section of the lamina. This pattern of cell lincage indicates that primordial cells contribute more or less equally to the growth of the axis, in contrast to the more traditional view of leaf development in which the leaf is pictured as arising from a group of apical initials. Clones induced prior to the initiation of the lamina demonstrate that the subepidermal layer of the lamina arises from at least six files of cells. Submarginal cells usually divide with their spindles parallel to the margin, and therefore contribute relatively little to the transverse expansion of the lamina. During the expansion of the lamina the orientation and frequency of cell division are highly regulated, as is the duration of meristematic growth. Initially, cell division is polarized so as to produce lineages that are at an oblique angle to the midrib; later cell division is in alternating perpendicular planes. The distribution of clones generated by irradiation at various stages of development indicates that cell division ceases at the tip of the leaf when the leaf is about one tenth its final size, and then ceases in progressively more basal regions of the lamina. Variation in the mutation frequency within the lamina reflects variation in the frequency of mitosis. Prior to the mergence of the leaf the frequency of mutation is maximal near the tip of the leaf and extremely low at its base; after emergence, the frequency of mutation increases at the base of the leaf. In any given region of the lamina the frequency of mutation is highest in interveinal regions, and is relatively low near the margin. Thus, both the orientation and frequency of cell division at the leaf margin indicate that this region plays a minor role in the growth of the lamina.Abbreviation MF mutation frequency  相似文献   

6.
Mepiquat chloride (N, N-dimethylpiperidinium chloride), well known as PIX, is a potential systemic plant growth regulator. The effects of PIX on plant height, stem elongation, leaf area, net photosynthetic rates, chlorophyll content, sucrose and starch levels, and RuBP carboxylase activity in cotton (Gossypium hirsutum L. cv. DES 119) plants were measured. PIX was sprayed (0, 7.65, 15.3, 30.6 or 61.2 g active ingredient ha–1) on the plants at first square (25 days after emergence) and measurements were made at frequent intervals. Plant height was clearly reduced by PIX. The total length of vegetative branches and fruiting branches was 40% and 50% less than the control. Total leaf area in PIX treated plants was 16% less than the control. Net photosynthetic rates were 25% less in PIX-treated leaves. PIX treated leaves had more chlorophyll content. The activity of RuBP carboxylase was decreased in PIX treated plants. Starch accumulation was noticed in PIX treated leaves while sucrose content was not changed. The data reported here suggest that reduced growth responses induced by PIX results in partial loss of photosynthetic capacity in cotton at least up to 20 days after application of the growth regulator.  相似文献   

7.
The effect of increasing spore concentration of Alternaria solani (Early blight disease) on the shoot growth of young tomato plants was analysed. Changes in growth were related to the severity of infection which increased with increasing inoculum. Leaf production was not affected but dry weights and especially leaf expansion were decreased. The effective leaf areas of the five inoculated leaves (L1-L5 numbered from the plant base) were drastically decreased by expanding necrotic lesions and, to a lesser extent, by premature leaf fall. Healthy leaves expanding soon after inoculation (L6, L7) were markedly affected by the disease on the lower leaves and had decreased specific leaf areas (ratio of leaf area to leaf dry weight) but later formed (from L8) leaves were less affected and had greater specific leaf areas than equivalent leaves on uninoculated plants.  相似文献   

8.
The subcellular distribution of hexoses, sucrose and amino acids among the stromal, cytosolic and vacuolar compartments was analysed by a nonaqueous fractionation technique in leaves of tobacco (Nicotiana tabaccum L.) wild-type and transgenic plants expressing a yeast-derived invertase in the cytosolic, vacuolar or apoplasmic compartment. In the wild-type plants the amino acids were found to be located in the stroma and in the cytosol, sucrose mainly in the cytosol and up to 98% of the hexoses in the vacuole. In the leaves of the various transformants, where the contents of hexoses were greater than in wild-type plants, again 97–98% of these hexoses were found in the vacuoles. It is concluded that leaf vacuoles contain transporters for the active uptake of glucose and fructose against a high concentration gradient. A comparison of estimated metabolite concentrations in the subcellular compartments of wild-type and transformant plants indicated that the decreased photosynthetic capacity of the transformants is not due to an osmotic effect on photosynthesis, as was shown earlier to be the case in transformed potato leaves, but is the result of a long-term dedifferentiation of tobacco leaf cells to heterotrophic cells.Abbreviations apo-inv tobacco plant with yeast invertase in the apoplasm - Chl chlorophyll - cy-inv tobacco plant with yeast invertase in the cytosol - vac-inv tobacco plant with yeast invertase in the vacuole - WT wild-type tobacco plant The authors thank A. Großpietsch for her able technical assistance. This work has been supported by the Bundesminister für Forschung und Technologie.  相似文献   

9.
The distribution and mobility of manganese (Mn) in the hyperaccumulator plant species Phytolacca acinosa Roxb. (Phytolaccaceae) were investigated in a hydroponic system. The plants were exposed to 2 or 5 mM Mn for up to 28 days. For any given plant, the Mn content in the mature leaves (nos. 5–9) was always higher than that in the old (nos. 1–4) and young leaves (nos. 10–14). Within the different parts of a leaf, Mn was preferentially accumulated in the leaf marginal area, where the observed level was threefold higher than that in the midrib. Cross-sectional analysis of the leaf revealed that the concentration of Mn was higher in the leaf epidermis than in the mesophyll. Cell fractionation analysis with P. acinosa leaves showed that most of the Mn (78.4%) was present in the final supernatant fraction (following centrifugation at 20,000 g for 45 min). The distribution of Mn in the leaves of P. acinosa was controlled mainly by the transpiration rate. Our investigation demonstrated that Mn was readily transported from the roots to shoots of P. acinosa but that it could not be remobilized readily after it reached leaves.  相似文献   

10.
Using field assays of leaf preference, we tested the hypothesis that wilting affects the selection of leaves by the leaf-cutting ant Atta laevigata (Fr. Smith). Detached leaves were left to air-dry until noticeably wilted. The area removed by the ants from wilted leaves was significantly greater than the area removed from fresh leaves, this effect being observed in several plant species, in leaves of different age, and in assays with different ant colonies. Leaves collected from water-stressed plants were also preferred to leaves from non-stressed plants. A. laevigata was found to employ a two-stage, size-related, strategy when cutting plants. Larger workers climbed the plant stem and dropped whole leaves to the ground by severing their petioles; smaller workers cut the lamina of the dropped leaves. The ants frequently left dropped leaves on the ground, until the next foraging day or even later, when they were harvested in a wilted condition in preference to newly-dropped leaves.It is possible that during wilting some repellent substances evaporate or become less effective, thus enhancing leaf palatability. Alternatively or in addition, changes in nutrient and water content may have rendered wilted leaves more palatable to leaf-cutting ants.  相似文献   

11.
3种寄主上桃蚜的选择性及形态分化   总被引:4,自引:0,他引:4  
桃蚜是一种重要的农业害虫,寄主广泛,种下分化复杂。以采自黄土高原旱作区桃树、烟草、甘蓝上的桃蚜为研究对象,通过叶片选择法、传统比较形态测定法研究了3种寄主上桃蚜的选择性及形态分化。结果表明:在3种寄主同时存在的情况下,烟草上的桃蚜嗜食烟草,表现为63.5%的桃蚜选择烟草叶,13.8%选择甘蓝叶,8.2%选择桃叶,而甘蓝和桃树上的桃蚜对原寄主没有表现出明显的嗜好性;从形态指标来看,3种寄主上的桃蚜在体长、触角末节长度、后足腿节长度、触角与体长的比例方面存在显著差异(P0.05),说明这几个特征可以作为区分这3种寄主上桃蚜的依据。综合分析可以初步认为黄土高原旱作区烟草上的桃蚜可能形成了寄主专化型-烟草型。  相似文献   

12.
Three contrasted genotypes of Musa spp. (M. acuminata cv Grande Naine, M. acuminata spp. Banksii and M. balbisiana spp. Tani) were grown for 6 weeks under optimal conditions in hydroponics and were submitted to a wide range of Si supply (0–1.66 mM Si) to quantify the Si uptake and distribution in banana, as well as the effect of Si on banana growth. The level of Si supply did not affect plant growth, nor the rate of water and nutrient uptake. The rate of Si uptake and the Si concentration in plant tissues increased markedly with the Si supply. At the highest Si concentrations (1.66 mM), silicon absorption was essentially driven by mass flow of water (passive transport). However, at lower Si concentrations (0.02–0.83 mM), it was higher than its uptake by mass flow and caused the depletion of silicon in the nutrient solution, suggesting the existence of active processes in silicon transport. The distribution of silicon among shoot organs (pseudostem < petiole and midrib < young lamina < old leaf) confirmed the major role of transpiration in silicon accumulation and was not dependent on silicon supply. However, other mechanisms of transport might be operating in the roots and in the petiole and midrib of young leaves, whose silicon concentration was unexpectedly high at low Si supply (0.02 mM) compared to higher levels of Si. The three genotypes did not exhibit consistent differences in their responses to silicon supply.  相似文献   

13.

Background and Aims

The water-transport capacity of leaf venation is positively related to the leaf-lamina area, because the number and diameter of vein-xylem conduits are controlled to match the lamina area. This study aimed to investigate how this co-ordinated relationship between the leaf-lamina area and vein-xylem characteristics is achieved by examining the midrib xylem of tobacco leaves.

Methods

The changes in the midrib-xylem characteristics over time were quantified using leaves with four different final lamina areas. The measured data were fitted to sigmoidal functions. From the constants of the fitted curves, the final values in mature leaves, maximal developmental rates (VDev) and developmental duration (TDev) were estimated for each of the xylem characteristics. Whether it is the lamina or the midrib xylem that drives the co-ordinated development was examined by lamina removal from unfolding leaves. The effects of the application of 0·1 % IAA (indole-3-acetic acid) to leaves with the laminas removed were also analysed.

Key Results

For both the leaf lamina and the midrib-xylem characteristics, the differences in final values among leaves with different lamina areas were more strongly associated with those in VDev. Notably, the VDev values of the midrib-xylem characteristics were related to those of the leaf-lamina area. By lamina removal, the conduit diameter was reduced but the number of conduits did not significantly change. By IAA application, the decrease in the conduit diameter was halted, and the number of conduits in the midrib xylem increased.

Conclusions

According to the results, the VDev values of the lamina area and the midrib-xylem characteristics changed in a co-ordinated manner, so that the water-transport capacity of the midrib xylem was positively related to the leaf-lamina area. The results also suggest that IAA derived from the leaf lamina plays a crucial role in the development of the leaf venation.  相似文献   

14.
Summary Individual leaves of eastern cottonwood (Populus deltoides Bartr.), representing an ontogenetic series from leaf plastochron index (LPI) 3.0 to 8.0, were fed 14CO2 and harvested after 2–24 h. Importing leaves from LPI-1.0 through 8.0 on each plant were sectioned into 9 parts, and each part was quantitatively assayed for 14C activity. The highest level of 14C import was by leaves from LPI 1.0 to 3.0, irrespective of source-leaf age. 14C was translocated preferentially to either the right or left lamina-half depending on the position of the importing leaf in the phyllotactic sequence and its stage of development. For example, import was high when the importing leaf and the source leaf had two vascular bundles in common, moderately high with one bundle in common, and low with no bundles in common. The distribution of 14C within young importing leaves was highest in the lamina tip and decreased toward the base. With increasing leaf age, incorporation declined in the lamina tip and increased in the base.It may be concluded that each cottonwood leaf progresses through a continuum of importing and exporting stages as its lamina expands. The photosynthate imported by a given leaf is compartmentalized, with different exporting leaves supplying photosynthate to rather restricted regions of the lamina. Such localization within the importing leaf depends on its vascular connections with each of the exporting leaves, and these are predictable from a knowledge of the phyllotaxy.Plant Physiologists.  相似文献   

15.
The effects of water stress and subsequent re-hydration on growth, leaf abscission, photosynthetic activity, leaf water potential and ion content were investigated in papaya seedlings (Carica papaya L.) cv. “Baixinho de Santa Amalia”. Water stress was imposed by suspending irrigation during 34 days. Thereafter, plants were regularly re-watered. Drought arrested plant growth, induced leaf abscission and drastically decreased photosynthetic rate. However, leaf water potential was hardly reduced. Water deficit also induced sodium, potassium and chloride accumulation in leaves and roots, and did not modify nitrogen levels in both organs. Re-hydration stimulated growth, promoted emergence of new leaves, reactivated photosynthetic machinery function and reduced ion content to control levels. The results indicated that the ability of papaya plants to improve drought tolerance is not mediated through the reduction of leaf abscission, the detention of growth or the decrease of net CO2 assimilation. In contrast, the data suggested that under water stress conditions these plants appear to posses a certain capacity to increase ion content, which might contribute to osmotic adjustment.  相似文献   

16.
A winter variety (Miranovskaja 808) of wheat (Triticum aestivum L.) was grown in water cultures containing 0, 5, 15, 30, and 60 mM choline chloride. There was an increase in the phosphatidylcholine level at the expense of phosphatidic acid, parallel with an increasing choline concentration in the cultivating medium. While the ratio of free sterols to phospholipids remained essentially constant in the seedlings, there was an increase in the ratio of saturated-to-unsaturated fatty acids of the phospholipid fraction. Probing the protoplasts obtained from the leaves of the seedlings, with spin labeled 5-doxyl- and 16-doxyl stearic acids, indicated a progressive rigidifying of the hydrophobic core of the plasmalemma. It is suggested that this is a manifestation of compensatory mechanisms by which plants attempt to maintain unchanged average membrane fluidity across their membranes in response to the fluidizing effect of choline head groups.  相似文献   

17.
Development of the Populus leaf is presented as a model system to illustrate the sequence of events that occur during the sink to source transition. A Populus leaf is served by three leaf traces, each of which consists of an original procambial trace bundle that differentiates acropetally and continuously from more mature procambium in the stem and a complement of subsidiary bundles that differentiates bidirectionally from a leaf basal meristem. During development these subsidiary bundles maintain continuity through the meristematic region of the node. The basipetally developing subsidiary bunles form phloem bridges that serve to integrate adjacent leaf traces of the stem vasculature. Distal to the node the acropetally developing bundles from all three leaf traces are reoriented in a precise and orderly sequence to form tiers of petiolar bundles. These tiers of bundles extend into the midrib where bundles diverge at intervals as the major lateral veins. The dorsal-most tier of bundles extends to the lamina tip and each successive tier of bundles contributes to lateral veins situated more proximally in the lamina. Although the midrib and the major vein system differentiate acropetally in the lamina, they mature basipetally. Maturation of the mesophyll and other lamina tissues also mature basipetally. As a consequence of the basi-petal maturation process, the lamina tip matures very early and begins exporting photosynthates while the lamina base is still importing from other leaves. The transition of a leaf from sink to source status must therefore be considered as a progression of structural and functional events that occur in synchrony.  相似文献   

18.
Sucrose synthase (EC 2.4.1.13) activity in young growing leaves was highest in the leaf base in eggplants ( Solanum melongena L.), cassava ( Manihot esculenta Crantz), grapevine ( Vitis vinifera L.), and in the leaf sheath of sugar cane ( Saccharum of ficinarum L.) and maize ( Zea mays L.). In addition, increasing sucrose synthase activity was measured towards the edge of growing eggplant leaves while the activity in mature leaves was highest in the midrib. The activity of acid and alkaline invertase was very low in the midrib but higher in the blade of fully expanded eggplant leaves. Highest invertase activities were found in younger growing leaves. It was concluded that in growing leaves a close relationship might exist between the activity of sucrose synthase and the import of sucrose from source leaves.
Detachment of mature eggplant leaves led to a 2- to 3-fold increase of sucrose synthase activity in blade and midrib of these leaves. In contrast, invertase activity decreased after detachment in both leaf blade and midrib. It was concluded that the rise in sucrose synthase activity might have been caused by the observed increase of sucrose concentration in detached leaves and that sucrose synthase might have an important role in the regulation of sucrose content of the conducting tissue.  相似文献   

19.
The effect of pretreatment of cucumber (Cucumis sativus L.) roots with choline chloride or ethanolamine on leaf phospholipid composition and light-induced leaf damage during chilling was studied. Photooxidative chlorophyll degradation was similarly inhibited by both amino alcohols. The decrease of the chlorophyll a/chlorophyll b ratio and the increase of polyunsaturated-fatty-acid degradation during chilling in the light were equally inhibited by pretreatment with choline chloride or ethanolamine. Treatment with choline chloride and ethanolamine caused, respectively, 43% and 26% increases in the total phospholipid contents of the leaves. After treatment with choline chloride, the phosphatidylcholine content was higher than the content of phosphatidylethanolamine; the reverse was true after treatment with ethanolamine. The chlorophyll concentration increased less than the phospholipid concentration, resulting in a decreased chlorophyll/phospholipid ratio of treated leaves. During chilling in the light, degradation of phosphatidylcholine, ethanolamine and phosphatidyl glycerol occurred. Phosphatidyl glycerol was less sensitive than phosphatidylcholine and ethanolamine. The degradation was equally inhibited by pretreatment with either amino alcohol. Possible connections between the phospholipid content of leaf membranes and the inhibition of chilling-induced photooxidative leaf damage are discussed.Abbreviations CC choline chloride - Chl chlorophyll - EA ethanolamine - PC phosphatidyl choline - PE phosphatidyl ethanolamine - PG phosphatidyl glycerol  相似文献   

20.
The analysis of diurnal oscillatory movements of tobacco leaves was used in the diagnosis of viral infection of plants. The oscillatory helices circumscribed by a growing leaf of a healthy plant were regular, but some deviations, particularly in the transition points, were recorded. In order to make clear the cause of these irregularities of trajectory, the course of elongation of leaf petiole and blade in relation to localization and shift of zones of elongation during ontogenesis was analysed. The present analysis is similar to that described by the author's earlier experiments with pea roots. Oscillatory curves circumscribed by petiole, projected on a horizontal plane, were compared with curves circumscribed by the blade tip. The analysis of the leaves of different ages enabled us to study this process in dependence on growth rate. It was confirmed that oscillations are a result of elongation; the extent of oscillations is quantitatively dependent on the growth rate. An analysis of the zones of growth showed that in petiole the active meristems are localized near to its base while in the leaf lamina they move gradually during the ontogenesis from the apical to the basal part of the leaf blade. Active meristems of young rapidly growing leaves are localized approximately in the middle of the blade while those of old leaves were found in close proximity to the base of the lamina. The growth rate of petiole can be expressed in hundreds of mm per hour (4.8×10?2 mm h?1); half of this value was recorded for its apical part. The growth rate of leaf blade was found approximately ten times higher (3.2×10?1 mm h?1). The oscillatory movements of growing leaf consists of two integrate components: of oscillations originating in the base of the petiole and of oscillations of leaf blade the centrum of which is localized in the basal third of the blade. The arrangement of the experiments did not enable us to determine to what extent the phototropic response of leaf blade participates in leaf movements. The movements of leaves of an intact plant are evidently affected by rhythmic stem oscillations. Stem is an integral part of a system which participates in the transfer of information in plants.  相似文献   

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