The emergy ecological footprint for small fish farm in China

Mariculture, especially cage aquaculture remains a growing, vibrant and important production sector for high protein food in coastal regions in China. A quantitative evaluation of the mariculture system is an essential step to documenting its’ sustainability. The method of emergy ecological footprint is applied to evaluate the environmental sustainability of an offshore small fish farm (Great Marine fish farm, GMFF) in the East China Sea. All input needed to support fish farming were accounted and converted into biological space, to estimate the natural capital demand for the rearing process in terms of global hectares. The emergy ecological footprint of GMFF was 1953.19 ha, meaning that nearly 2000 ha of ecologically productive lands were needed to support the fish framing. The largest component of emergy ecological footprint was forage (1183.64 ha), which took up 60.60% of the total footprint; the second and third largest footprint components were fingerlings and fuel. In a word, emergy ecological footprint can serve as a practical and meaningful tool for comparing and monitoring the environmental impact of fish farming. The strong dependence of external contributions of exploiting the wild fish resources affects strongly the level of environmental sustainability of fish farming.     

Emergy synthesis of a combined food and energy production system compared to a conventional wheat (Triticum aestivum) production system

It is a major challenge to produce food and energy sustainably for the ever increasing world population as today's conventional food production and energy needs are met by the fossil based resources, causing enormous environmental load. A novel, combined food and energy (CFE) agro-ecosystem, was designed for sustainable production of food, fodder and energy without chemical inputs (fertiliser, herbicide and fungicide). The objective was an emergy synthesis of the CFE system compared to a conventional wheat (Triticum aestivum) production system to assess resource use efficiency. The emergy indices, used to assess the environmental performance and sustainability, exhibited contrasting differences between the two production systems in terms of outputs (Y), total emergy use, solar transformity, relative use of local renewable resources, environmental loading ratio (ELR), emergy yield ratio (EYR) and emergy sustainability index (ESI). The Y in the CFE consisted of grain, straw, fodder and woodchip production of 4020, 3580, 6100 and 10,000 kg/ha/yr respectively whereas Y in the conventional wheat consisted of 7250 and 3770 kg grain and straw/ha/yr respectively. The Y in the CFE was 81% (2.80E+11 J/ha/yr) higher with 13.5 times (6.40E+03 seJ/J) lower solar transformity compared to the Y (1.54E+11 J/ha/yr) and solar transformity (8.63E+04 seJ/J) in the conventional wheat, exhibiting highly resource intensive production in conventional wheat. The local renewables constituted 19.2% and 2.6% of the total emergy input in the CFE and the conventional wheat respectively with a corresponding lower ELR (4.21) and 22.5% higher EYR (1.26) in CFE compared to conventional wheat. CFE was more reliant on local renewable emergy flows and compatible with the local environment with higher ESI (0.30) compared to conventional wheat (0.03), where 64.5% of the total emergy input constituted chemical inputs. The study demonstrated that the innovative agro-ecosystem, exemplified by CFE, is considerably less resource demanding and more amenable to sustainable production, whether defined in terms of outputs, solar transformity, relative use of local renewable resources, EYR, ELR or ESI.     

The emergy of metabolism in different ecosystems under the same environmental conditions in the agro-pastoral ecotone of northern China

The sustainability of ecosystem productivity and rules governing ecosystem development are important topics of scientific research. The emergy approach is an effective method for investigating these topics, especially when used to evaluate systems that have developed under the same environmental conditions, such as climate and soil. In this paper, emergy differences between terrestrial ecosystems were studied in Guyuan County, a region representative of the agro-pastoral ecotone in Hebei Province, China. A combination of field tests and a questionnaire survey were carried out between June and August 2015. The ecosystems studied included natural grassland, artificial grassland, field crops and commercial crops. These four ecosystems were further subdivided into a total of ten ecosystems. Natural grassland was divided into free-grazing and mowed ecosystems; artificial grassland consisted of oat, Chinese leymus and corn silage; field crops included naked oats, flax and wheat; and commercial crops consisted of cabbage and potatoes. The results showed that the rain input of 4.78 × 10¹⁴ seJ/ha/yr constituted the highest renewable natural resource emergy and that the purchased emergy inputs of the ten ecosystems ranged from 3.53 to 147.67 × 10¹⁴ seJ/ha/yr. Natural resource emergy input was the basic power to maintain the ecosystem, and purchased emergy input was the direct cause of the development of the ecosystems. Groundwater was the most important non-renewable purchased energy for the production of economic crops. The emergy investment ratios (EIR) for potatoes (27.81) and cabbage (19.03) were higher than those of the other ecosystems, but mowed and artificial Chinese leymus grassland had the higher emergy self-sufficiency rates (ESR). Natural grassland, artificial Chinese leymus grassland and traditional grain crops had a low environmental load and high sustainability, whereas potatoes and cabbage had a high environmental load and low sustainability. Overall, rain-fed artificial grassland has a high development potential from the perspective of environment and productivity.     

Emergy and economic evaluations of four fruit production systems on reclaimed wetlands surrounding the Pearl River Estuary,China

Emergy and economic methods were used to evaluate and compare a traditional tropical fruit cultivation system, for bananas, and three newly introduced fruit cultivation systems, for papaya, guava and wampee, on reclaimed wetlands of the Pearl River Estuary, China. The goal of this study was to apply ecological engineering principles to fruit production system designs to maximize total emergy benefits and sustainability. The evaluations considered input structure, production efficiency, environmental impacts, economic viability and sustainability. The market effects on emergy exchange were assessed both for purchasing the inputs to production and for selling the fruit. These market effects were also considered in the evaluation of sustainability by using the Emergy Index for Sustainable Development (EISD), which was evaluated with and without taking the change in natural capital (i.e., soil organic matter) into consideration. The results showed that all three of the newly introduced systems are much more sustainable than the traditional banana production system. The guava production system had the highest value of the Emergy Sustainability Index (ESI = 0.40). The high price of wampee gave it the highest economic yield/cost ratio (4.87) and EISD (0.73). Emergy and economic evaluations are complementary methods, with emergy analysis shedding more light on environmental support and impacts of the production systems not considered in the market value, and economic analysis focusing on the effects of markets on fruit production. The Emergy Exchange Ratio (EER) was proposed as a bridge between emergy and economic evaluations for specific systems and/or processes.     

Conversion of cassava flour to fuel ethanol by sequential solid state and submerged cultures

A process for conversion of cassava flour to ethanol was developed. This involved direct inoculation of Aspergillus awamori spores into a cassava flour paste and incubation for some period during which hydrolytic enzymes are produced (solid state culture or koji production) and subsequent addition of water and yeast cells, during which there is simultaneous hydrolysis and ethanol production (submerged culture). When cassava flour alone was used for the solid state phase, the paste was very sticky, making mixing and aeration difficult. However, addition of rice bran improved the texture and enzyme production. The optima rice bran concentration, spore inoculum concentration, and duration of solid state culture before submerged culture were 20%, 6.16 × 10⁶ spores/100 g, and 2 days, respectively. Under these optimum conditions, a high ethanol concentration of 120 g/L and ethanol yield of 0.309 g-ethanol/g-cassava flour were obtained. This ethanol yield corresponds to 0.44 g-ethanol/g-cassava starch.     

Energy budgeting and emergy synthesis of rainfed maize–wheat rotation system with different soil amendment applications

Soil is a non-renewable resource and its preservation is essential for food security, ecosystem services and our sustainable future. Simultaneously, it is a major challenge to substitute non-renewable fossil based resources with renewable resources to reduce environmental load. In order to check soil erosion vis-a-vis degradation of sloppy lands of rainfed maize–wheat rotation system, fertilization with organic manure supplemented with inorganic fertilizers is required. In order to address these issues, substitution of 50% NPK through four organic manures viz. farmyard manure (FYM), vermicompost (VC), poultry manure (PM) and in situ green manuring (GM) of sunnhemp (Crotalaria juncea L.) were evaluated against 100% NPK through inorganic fertilizers and through FYM for energy budgeting and emergy synthesis during 2009–2014. Integrated use of FYM along with 50% NPK fertilizers could maintain the highest energy ratio (7.3), human energy profitability (142.4), energy productivity (0.22 kg MJ⁻¹), and energy profitability (6.3 MJ ha⁻¹) over other treatments. However, GM and inorganic fertilizers on equal NPK basis maintained the highest energy intensiveness (24.61 MJ US $⁻¹) and exhibited higher emergy yield ratio (2.66) and lower emergy investment ratio (0.60) and environmental loading ratio (3.74) which resulted into higher environmental sustainability index (0.71) over other treatments. Fertilization with organic manure (FYM) alone could not compete with other fertilized options to energy budgeting and emergy synthesis except specific energy. The study demonstrated that innovative integrated nutrient management of chemical fertilizers and organic manures particularly FYM for energy budgeting and GM for emergy synthesis may be considered as feasible and environment-friendly options for soil conservation, thereby benefiting a 50% saving on costly chemical fertilizers in non-OPEC countries which import most of its phosphorus and potassium fertilizers.     

Feasibility of using constructed treatment wetlands for municipal wastewater treatment in the Bogotá Savannah,Colombia

The main water bodies in the Bogotá Savannah have been seriously polluted due to the mismanagement of domestic, agricultural, and industrial wastewater. While there are a number of wastewater treatment facilities in the region, most do not function properly. There is a great need for inexpensive and sustainable wastewater treatment systems that are not technologically sophisticated and that do not require intensive management. The main goal of this study was to quantify the performance and sustainability of treatment wetlands and existing wastewater treatment systems in this region. Using data from the literature, a treatment wetland model was developed, which focused on pollutant removal. The modeled performance was compared to a system of waste stabilization ponds and a sequencing batch reactor. The three systems were subject to cost analysis and an emergy evaluation, leading to the assessment of indicators of cost-benefit for comparison. The economic analysis suggested that the net annual cost of the treatment wetland was US$ 14,672, compared to US$ 14,201 for the stabilization ponds and US$ 54,887 for the batch reactor. The emergy evaluations show that the ponds have the lowest annual emergy flow (6.65 + 16 sej/yr), followed by the constructed wetland (2.88E+17 sej/yr) and the batch reactor (8.86E+17 sej/yr). These results were combined to estimate treatment ratios (contaminants removed per lifetime cost, and contaminants removed per total emergy), cost ratios (cost per volume of water, annual cost per capita, and construction cost per capita), and emergy ratios (treatment yield, renewable emergy, lifetime emprice, construction emprice, non-renewable emergy, empower density, environmental loading, total emergy per volume of water, and emergy per capita).     

Emergy-based indicators of regional environmental sustainability: A case study in Shanwei,Guangdong, China

Understanding which factors mainly affect the environmental sustainability of a Region is a very crucial concern for many Chinese planners and policy-makers. In this study, the emergy accounting method is applied to the production and consumption activities of the city of Shanwei, a seaside urban area in Guangdong region, China. The emergy method provides a comprehensive assessment of a system, by generating a consistent set of indicators capable to capture the dynamics of the system as a whole and its efficiency in converting resources into an economic product and welfare. At the same time, the approach is capable to provide details about a system's performance over time, its use of renewable versus nonrenewables sources, local versus imported, concentrated versus dispersed and finally labor intensive versus fuel intensive processes. The main local renewable and nonrenewable emergy flows supporting the investigated area were included in the accounting, together with the flows of imported energy, goods, machinery, labor and services. Coal plays a crucial role for electricity generation, industrial uses, but also gasoline and diesel for transport affect the overall sustainability to a non-negligible extent. An Emergy Sustainability Indicator (ESI) of the local economic and social system is calculated and compared with the average value of the Chinese economy, based on the assumption that a process or system is unsustainable if it contributes to lower the overall sustainability of the region or country. This is quantified by the requirement that always must be ESI_local ≧ ESI_country. Results confirm that the most crucial factor for this region sustainability is the use of coal as a non-renewable and imported energy source. The calculated emergy indicators translate into a set-aside buffer land demand capable to mitigate, dilute, and uptake the environmental impact of human-dominated activities. Unfortunately, such buffer land demand is much higher than the land actually available. As a result, both the ESI-based sustainability equation as well as the lack of support land make the city's production and consumption patterns unsustainable and call for different lifestyles and environmental policies.     

Emergy and exergy evaluation of a dike-pond project in the drawdown zone (DDZ) of the Three Gorges Reservoir (TGR)

The dike-pond system was a form of ecological engineering that was a component of successional dikes and ponds along the banks of the Pengxi River in the drawdown zone (DDZ) of the Three Gorges Reservoir (TGR). The application of science-based evaluation system was appropriate for the flows of emergy in this agricultural ecosystem. The Emergy Analysis (EmA) has the ability to transform different types of inputs to a common form to allow meaningful comparisons across different systems. This study made use of the emergy analysis that assessed two different types of farming methods in the DDZ of the TGR. One method was planning crops in a dike-pond system (model I), and the other method was conventional agriculture (model II). In addition, the Exergies of both yields of agriculture methods were calculated, and the Exergy and Emergy Density (ED) were combined to explore the quality of these methods. The results showed that the two models relied on different resources. The ED yield of both models were similar, but the emergy investment of model II was greater than that of model I. Model II also used less renewable energy input to the agricultural systems than model I. The agricultural emergy sustainability index (AESI) of model I system (AESI = 2.4 > 1) was greater than that of model II system (AESI = 0.5 < 1), which indicated that the sustainable development of model I was stronger than that of model II in the DDZ of the TGR. The ratio Exergy/Emergy density (R_ex/em) in the two models of different agricultural system were 121.52 × 10⁻³ J/sej (Model I) and 24.19 × 10⁻³ J/sej (model II). Model I was greater than model II, and the result intimated that the model I agricultural system was a new method in the DDZ, but it was older and closer to the steady state than model II. The Emergy and Exergy analysis certifies that model I has a more acceptable and more sustainable development potential and is more stabilized in the DDZ of the TGR.     

An emergy evaluation of the sustainability of Chinese crop production system during 2000–2010

Crop production systems are the basis of human survival and development because they can produce grain and industrial raw materials. As one of the largest agricultural countries in the world, the sustainability of China's crop production system is being concerned widely with its economic development and increasing population. This study adopted emergy analysis to explore the comprehensive performance of this system. A set of emergy based indicator system was used to investigate its economic benefit, environmental pressure and sustainability from 2000 to 2010. The study results show that the purchased nonrenewable input makes the largest contribution to the total input (average value 60.73% of the total input), which mainly derived from agricultural mechanic equipments and chemical fertilizer; on the average, beans has the largest share (20.20%) to the total emergy output, next from rape seed (18.36%), then from peanuts (15.85%), fruits (15.74%), wheat (8.26%), rice (8.07%), corn (7.66%) and cotton (4.60%) accordingly, and the other four categories crops just have a contribution of 1.28%; the production efficiency of China's crop production system has been raised by 11.54% with decrease of the indicator unit emergy value of product (UEVP) from 1.82E09 sej/g to 1.61E09 sej/g, the dependence of this system on economic market has increased by 24.92% with growth of the indicator EIR from 6.22 to 7.77, its economic benefit has been reduced by 0.59% with decline of the indicator EYR from 1.69 to 1.68, and its environmental loading has raised by 57.89% with growth of the indicator ELR from 1.33 to 2.10; the sustainability of China's crop production system is reduced by 37.01% with decrease of the index ESI from 1.27 to 0.80, during this study period. Based on these study results, the following measures should be emphasized in future, including raising the efficiency of purchased non-renewable resources (especially agricultural mechanical equipments and chemical fertilizer), using other methods of cultivation inherently more sustainable (e.g. replacing chemical fertilizer with organic fertilizer, recycling organic wastes, biological control of agricultural pests, use of local renewable energy, and more), strengthening supervision of the related industrial processes and further promoting agricultural environmental protection.     

Sustainable biomass production: A comparison between Gross Energy Requirement and Emergy Synthesis methods

In this paper two methods for energy analysis and environmental accounting (Gross Energy Requirement and Emergy Synthesis) are critically discussed in order to explore their ability to provide a comprehensive evaluation of the performance and environmental sustainability of human-dominated production processes. In order to allow a quantitative comparison, two cropping systems, namely 1 ha of corn production in Italy, and 1 ha of willow production in Sweden, are investigated by means of the parallel application of both methods. The case studies are carried out by performing a quantitative inventory of both natural and economic input flows to the investigated cropping systems. Such input flows are then converted into embodied energy (MJ) as well as emergy (seJ) units. Finally, performance indicators representative for each method are calculated. Results provided by the two methods and their respective theoretical features are compared and discussed in order to point out limits and potentialities of both approaches. The study shows that the two methods account for different – although complementary – categories of input flows, use different conversion factors, and answer to different questions and concerns. Gross Energy Requirement focuses on fossil fuel use and is capable to support the development of more efficient use of commercial energy. Emergy Synthesis uses broader spatial and time frames and accounts for both natural and economic resources. In so doing, it takes into consideration different forms of energy, materials, human labor and economic services on a common basis, offering larger potentiality to explore the sustainable interplay of environment and economy.     

Enhanced solvent production by metabolic engineering of a twin-clostridial consortium

The efficient fermentative production of solvents (acetone, n-butanol, and ethanol) from a lignocellulosic feedstock using a single process microorganism has yet to be demonstrated. Herein, we developed a consolidated bioprocessing (CBP) based on a twin-clostridial consortium composed of Clostridium cellulovorans and Clostridium beijerinckii capable of producing cellulosic butanol from alkali-extracted, deshelled corn cobs (AECC). To accomplish this a genetic system was developed for C. cellulovorans and used to knock out the genes encoding acetate kinase (Clocel_1892) and lactate dehydrogenase (Clocel_1533), and to overexpress the gene encoding butyrate kinase (Clocel_3674), thereby pulling carbon flux towards butyrate production. In parallel, to enhance ethanol production, the expression of a putative hydrogenase gene (Clocel_2243) was down-regulated using CRISPR interference (CRISPRi). Simultaneously, genes involved in organic acids reassimilation (ctfAB, cbei_3833/3834) and pentose utilization (xylR, cbei_2385 and xylT, cbei_0109) were engineered in C. beijerinckii to enhance solvent production. The engineered twin-clostridia consortium was shown to decompose 83.2 g/L of AECC and produce 22.1 g/L of solvents (4.25 g/L acetone, 11.5 g/L butanol and 6.37 g/L ethanol). This titer of acetone-butanol-ethanol (ABE) approximates to that achieved from a starchy feedstock. The developed twin-clostridial consortium serves as a promising platform for ABE fermentation from lignocellulose by CBP.     

Subcritical water and dilute acid pretreatments for bioethanol production from Melaleuca leucadendron shedding bark

The feasibility of bioethanol production using the lignocellulose of the shedding bark of Melaleuca leucadendron (Paper bark tree) was investigated. The effects of pretreatment parameters (temperature, time and acid concentration) on the yields of sugars and inhibitors, and optimal pretreatment conditions were determined. At very low severity conditions (combined severity factor, CSF ≤ 0.335), 28% of xylan was recovered and this recovery increased with increasing CSF till it peaked to 64.4% (11.2 g xylose L⁻¹) at a CSF of 1.475. However, at CSF > 2.0, xylose yield declined due to degradation. Mild and progressive glucose yield was detected in prehydrolysate at CSF ≥ 1.514, and subsequent enzymatic hydrolysis allowed complete glucan solubilization. Implementing environmentally friendly subcritical water pretreatment at CSF ≤ 0.335 on the shedding bark, about 85% of glucan solubilization was achieved after enzymatic hydrolysis. An industrial Saccharomyces cerevisiae strain readily fermented crude hydrolysate within 12 h, yielding 24.7 g L⁻¹ ethanol at an inoculum size of 2% (v/v), representing a glucose to ethanol conversion rate of 0.475 g g⁻¹ (91% ethanol yield). Based on our findings, the shedding bark is a potential feedstock for bio-ethanol production.     

Challenges in enzymatic hydrolysis and fermentation of pretreated Arundo donax revealed by a comparison between SHF and SSF

The perennial herbaceous crop Arundo donax is a potential feedstock for second-generation bioethanol production. In the present work, two different process options were investigated for the conversion of two differently steam-pretreated batches of A. donax. The pretreated raw material was converted to ethanol with a xylose-consuming Saccharomyces cerevisiae strain, VTT C-10880, by applying either separate hydrolysis and fermentation (SHF) or simultaneous saccharification and fermentation (SSF). The highest overall ethanol yield and final ethanol concentration were achieved using SHF (0.27 g g^?1 and 20.6 g L^?1 compared to 0.24 g g^?1 and 19.0 g L^?1 when SSF was used). The performance of both SHF and SSF was improved by complementing the cellulolytic enzymes with hemicellulases. The higher amount of acetic acid in one of the batches was shown to strongly affect xylose consumption in the fermentation. Only half of the xylose was consumed when batch 1 (high acetic acid) was fermented, compared to that 94% of the xylose was consumed in fermentation of batch 2 (lower acetic acid). Furthermore, the high amount of xylooligomers present in the pretreated materials considerably inhibited the enzymatic hydrolysis. Both the formation of xylooligomers and acetic acid thus need to be considered in the pretreatment process in order to achieve efficient conversion of A. donax to ethanol.     

Steam–air fluidized bed gasification of distillers grains: Effects of steam to biomass ratio,equivalence ratio and gasification temperature

In this study, thermochemical biomass gasification was performed on a bench-scale fluidized-bed gasifier with steam and air as fluidizing and oxidizing agents. Distillers grains, a non-fermentable byproduct of ethanol production, were used as the biomass feedstock for the gasification. The goal was to investigate the effects of furnace temperature, steam to biomass ratio and equivalence ratio on gas composition, carbon conversion efficiency and energy conversion efficiency of the product gas. The experiments were conducted using a 3 × 3 × 3 full factorial design with temperatures of 650, 750 and 850 °C, steam to biomass ratios of 0, 7.30 and 14.29 and equivalence ratios of 0.07, 0.15 and 0.29. Gasification temperature was found to be the most influential factor. Increasing the temperature resulted in increases in hydrogen and methane contents, carbon conversion and energy efficiencies. Increasing equivalence ratio decreased the hydrogen content but increased carbon conversion and energy efficiencies. The steam to biomass ratio was optimal in the intermediate levels for maximal carbon conversion and energy efficiencies.     

Ecological energy accounting for the gambling sector: A case study in Macao

Macao is a population-dense society that depends heavily on exogenous natural resources. In parallel with an expanding gambling sector, tourism became the most important driving force in Macao's economy. Economic growth in Macao has now benefited from the gambling boom and associated tourism for more than two decades. Although traditional economic theory has been used to deal with the economics of the gambling sector, the materials and energy foundation for this sector has not been previously analyzed. This is an important omission from the literature, since the gambling sector provides gamblers with food, tickets, services, water, electricity, equipment, labor, and other services that consume large quantities of materials and energy. In this paper, emergy synthesis is introduced to analyze Macao's gambling sector in 2004. Macao's casinos provide a dense flow of services that favor gambling tourists, since the emergy/$ ratio (1.42 × 10¹² sej/$) for the sector is much lower than that for Macao as a whole (2.38 × 10¹² sej/$). The emergy imports by this sector totaled 78.1 × 10²⁰ sej, and its exports were 77.1 × 10²⁰ sej. The emergy of services therefore plays an important role in Macao's gambling sector, and the transformity of an employee in this sector (11.2 × 10¹⁶ sej/person) was much higher than that of a typical person in Macao (5.27 × 10¹⁶ sej/person).     

Application of an integrated statistical design to optimize the cold enzyme hydrolysis conditions for ethanol production

Cold enzyme hydrolysis was investigated on the ethanol production by Saccharomyces cerevisiae during simultaneous saccharification and fermentation (SSF) processing. An integrated statistical design, which incorporated single factor design, response surface methodology (RSM) and weighting coefficient method, was used to determine the optimum hydrolysis conditions leading to maximum biomass, ethanol concentration and starch utilization ratio. After the studied ranges of α-amylase, glucoamylase and liquefaction time were identified by single factor design, RSM was used to further optimize the hydrolysis conditions for each objective. The results showed that, under hydrolysis condition optimized with RSM, biomass, ethanol concentration and starch utilization ratio reached 4.401 ± 0.042 × 10⁸ cells/ml, 14.81 ± 0.23% (wt.%) and 94.52 ± 0.53%, respectively. Finally, multi-objective optimization (MOO) was applied to obtain a compromised result of three desirable responses by weighting coefficient methodology. Biomass of 4.331 ± 0.038 × 10⁸ cells/ml, ethanol concentration of 14.12 ± 0.21% (wt.%) and starch utilization ratio of 92.88 ± 0.21% were simultaneous obtained when hydrolysis at pH 5.9 for 114 min with 233 IU/g_starch α-amylase and 778 IU/g_starch glucoamylase. The optimized conditions were shown to be feasible and reliable through verification tests.     

Operation of a bioelectrochemical system as a polishing stage for the effluent from a two-stage biohydrogen and biomethane production process

Anaerobic bioenergy production processes including fermentative biohydrogen (BioH₂), anaerobic digestion (AD) and bioelectrochemical system have been investigated for converting municipal waste or various biomass feedstock to useful energy carriers. However, the performance of a microbial fuel cell (MFC) fed on the effluent from a two-stage biogas production process has not yet been investigated extensively in continuous reactor operation on complex substrates. In this study we have investigated the extent to which a microbial fuel cell (MFC) can reduce COD and recover further energy from the effluent of a two-stage biohydrogen and biomethane system. The performance of a four-module tubular MFC was determined at six different organic loadings (0.036–6.149 g sCOD L⁻¹ d⁻¹) in terms of power generation, COD removal efficiency, coulombic efficiency (CE) and energy conversion efficiency (ECE). A power density of 3.1 W m⁻³ was observed at the OLR = 0.572 g sCOD L⁻¹ d⁻¹, which resulted in the highest CE (60%) and ECE (0.8%), but the COD removal efficiency decreased at higher organic loading rates (35.1–4.4%). The energy recovery was 92.95 J L⁻¹ and the energy conversion efficiency, based on total influent COD was found to be 0.48–0.81% at 0.572 g sCOD L⁻¹ d⁻¹. However, the energy recovery by the MFC is only reported for a four-module reactor and improved performance can be expected with an extended module count, as chemical energy remained available for further electrogenesis.     

Engineering Clostridium acetobutylicum for production of kerosene and diesel blendstock precursors

Processes for the biotechnological production of kerosene and diesel blendstocks are often economically unattractive due to low yields and product titers. Recently, Clostridium acetobutylicum fermentation products acetone, butanol, and ethanol (ABE) were shown to serve as precursors for catalytic upgrading to higher chain-length molecules that can be used as fuel substitutes. To produce suitable kerosene and diesel blendstocks, the butanol:acetone ratio of fermentation products needs to be increased to 2–2.5:1, while ethanol production is minimized. Here we show that the overexpression of selected proteins changes the ratio of ABE products relative to the wild type ATCC 824 strain. Overexpression of the native alcohol/aldehyde dehydrogenase (AAD) has been reported to primarily increase ethanol formation in C. acetobutylicum. We found that overexpression of the AAD^D485G variant increased ethanol titers by 294%. Catalytic upgrading of the 824(aad^D485G) ABE products resulted in a blend with nearly 50 wt%≤C₉ products, which are unsuitable for diesel. To selectively increase butanol production, C. beijerinckii aldehyde dehydrogenase and C. ljungdhalii butanol dehydrogenase were co-expressed (strain designate 824(Cb ald-Cl bdh)), which increased butanol titers by 27% to 16.9 g L⁻¹ while acetone and ethanol titers remained essentially unaffected. The solvent ratio from 824(Cb ald-Cl bdh) resulted in more than 80 wt% of catalysis products having a carbon chain length≥C₁₁ which amounts to 9.8 g L⁻¹ of products suitable as kerosene or diesel blendstock based on fermentation volume. To further increase solvent production, we investigated expression of both native and heterologous chaperones in C. acetobutylicum. Expression of a heat shock protein (HSP33) from Bacillus psychrosaccharolyticus increased the total solvent titer by 22%. Co-expression of HSP33 and aldehyde/butanol dehydrogenases further increased ABE formation as well as acetone and butanol yields. HSP33 was identified as the first heterologous chaperone that significantly increases solvent titers above wild type C. acetobutylicum levels, which can be combined with metabolic engineering to further increase solvent production.     

Cellulose ethanol production from waste newsprint by simultaneous saccharification and fermentation using Saccharomyces cerevisiae KNU5377

A thermotolerant ethanol-fermenting yeast, Saccharomyces cerevisiae KNU5377, isolated from a sludge of a local industrial complex stream in Korea, was evaluated for its capability for lignocellulosic ethanol production from waste newsprint in high temperature. In this fermentation, most of dry-defibrated waste newspaper was first saccharified at 50 °C for 108 h using a commercial cellulase and, then with the last addition of dry-defibrated newsprints to the pre-saccharified broth, simultaneous saccharification and fermentation (SSF) of 1.0 L of reaction mixture was carried out at 40 °C, slowly being dropped from 50 °C, for further 72 h in a 5 L fermentor by inoculating the overnight culture of KNU5377. The maximum production of 8.4% (v/v) ethanol was obtained when 250 g (w/v)/L of dry-defibrated waste newspaper was used for ethanol production by SSF. These results suggest that S. cerevisiae KNU5377 is very useful for cellulose ethanol production by the SSF system.