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1.
B. Fofana J. P. Baudoin X. Vekemans D. G. Debouck P. du Jardin 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(2):202-212
Chloroplast DNA (cpDNA) diversity has been examined using PCR-RFLP and RFLP strategies for phylogenetic studies in the genus
Phaseolus. Twenty-two species, including 4 of the 5 cultivated species (P. lunatus L., the Lima bean; P. vulgaris L., the common bean; P. coccineus L., the runner bean and P. polyanthus Greenman, the year-bean), represented by 86 accessions were included in the study. Six PCR primers designed from cpDNA and
a total cpDNA probe were used for generating markers. Phylogenetic reconstruction using both Wagner parsimony and the neighbor-joining
method was applied to the restriction fragment data obtained from each of the molecular approaches. P. vulgaris L. was shown to separate with several species of largely Mesoamerican distribution, including P. coccineus L. and P. polyanthus Greenman, whereas P. lunatus L. forms a complex with 3 Andean species (P. pachyrrhizoides Harms, P. augusti Harms and P. bolivianus Piper) co-evolving with a set of companion species with a Mesoamerican distribution. Andean forms of the Lima bean are found
to be more closely related to the 3 Andean wild species than its Mesoamerican forms. An Andean origin of the Lima bean and
a double derivative process during the evolution of P. lunatus are suggested. The 3 Andean species are proposed to constitute the secondary gene pool of P. lunatus, while its companion allies of Mesoamerican distribution can be considered as members of its tertiary gene pool. On the basis
of these data, an overview on the evolution of the genus Phaseolus is also discussed.
Received: 1 May 1998 / Accepted: 13 July 1998 相似文献
2.
A. Maquet I. Zoro Bi M. Delvaux B. Wathelet J.-P. Baudoin 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(5-6):980-991
Genetic diversity and structure within a Lima bean (Phaseolus lunatus L.) base collection have been evaluated using allozyme markers. The results obtained from the analysis of wild and cultivated
accessions confirm the existence of Andean and Mesoamerican gene pools characterised by specific alleles. Wild and cultivated
accessions of the same gene pool are grouped. The Andean natural populations have a very limited geographic distribution between
Ecuador and northern Peru. The Mesoamerican wild form extends from Mexico up to Argentina through the eastern side of the
Andes. Andean and Mesoamerican cultivated accessions of pantropical distribution contribute substantially to the genetic diversity
of the Lima bean base collection. Population genetic parameters, estimated from allozymes, confirmed the predominant selfing
mating system of the Lima bean. The selfing mating system, the occurrence of small populations, and low gene flow lead to
an interpopulation gene diversity (DST=0.235) higher than the intrapopulation gene diversity (HS=0.032). On the basis of the results, guidelines are given to preserve and exploit the genetic diversity of this threatened
species. The results also confirm the independent domestication of the Lima bean in at least two centres, one of which is
located at medium elevation in the western valleys of Ecuador and northern Peru.
Received: 3 June 1997 / Accepted: 17 June 1997 相似文献
3.
Genetic variation inPhaseolus lunatus (Lima bean) was investigated at isozyme and DNA levels. Sixty cultivated accessions, including representatives of the Mesoamerican and Andean gene pools and intermediate types, were analyzed for variability at 17 isozyme loci. Some accessions were also examined for restriction fragment length polymorphism (RFLP) at the rDNA level. These data were used to construct two dendrograms showing clear separation in two distinct groups corresponding to each of the gene pools and an intermediate one probably representing a transitional group. 相似文献
4.
Sparvoli Francesca Lanave Cecilia Santucci Annalisa Bollini Roberto Lioi Lucia 《Plant molecular biology》2001,45(5):587-597
Lectin-related polypeptides are a class of defence proteins found in seeds of Phaseolus species. In Lima bean (P. lunatus), these proteins and their genes have been well characterized in the Andean morphotype, which represents one of the two gene pools of this species. To study the molecular evolution of the lectin family in Lima bean we characterized the polypeptides belonging to this multigene family and cloned the genes belonging to the Mesoamerican gene pool. The latter gene pool contains components similar to those of the Andean pool, namely: an amylase inhibitor-like (AIL), an arcelin-like (ARL) lectin and the less abundant Lima bean lectin (LBL). These proteins originate from an ancestor gene of the lectin type which duplicated to yield the lectin gene and the progenitor of ARL and AIL. In this species. ARL represents an evolutionary intermediate form that precedes AIL. Phylogenetic analysis supports an Andean origin for Lima bean. The molecular evolutionary studies were extended to the genes of common bean and demonstrated that true lectin genes and the ancestor of lectin-related genes are the result of a duplication event that occurred before speciation. Lima and common bean followed different evolutionary pathways and in the latter species a second duplication event occurred that gave rise, in Mesoamerican wild genotypes, to arcelin genes. 相似文献
5.
Martha L. Serrano-Serrano Jorge Hernández-Torres Genis Castillo-Villamizar Daniel G. Debouck María I. Chacón Sánchez 《Molecular phylogenetics and evolution》2010,54(1):76-87
The aims of this research were to assess the genetic structure of wild Phaseolus lunatus L. in the Americas and the hypothesis of a relatively recent Andean origin of the species. For this purpose, nuclear and non-coding chloroplast DNA markers were analyzed in a collection of 59 wild Lima bean accessions and six allied species. Twenty-three chloroplast and 28 nuclear DNA haplotypes were identified and shown to be geographically structured. Three highly divergent wild Lima bean gene pools, AI, MI, and MII, with mostly non-overlapping geographic ranges, are proposed. The results support an Andean origin of wild Lima beans during Pleistocene times and an early divergence of the three gene pools at an age that is posterior to completion of the Isthmus of Panama and major Andean orogeny. Gene pools would have evolved and reached their current geographic distribution mainly in isolation and therefore are of high priority for conservation and breeding programs. 相似文献
6.
M. M. Khairallah B. B. Sears M. W. Adams 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1992,84(7-8):915-922
Summary Previous examination of intraspecific mitochondrial DNA (mtDNA) diversity in common bean, Phaseolus vulgaris, showed that five restriction fragment length polymorphisms (RFLPs) distinguish the mitochondrial genomes of the two major gene pools of cultivated beans, the Mesoamerican and the Andean. In the study presented here, mtDNA was used to compare the amount of diversity in cultivated beans to that in collections of wild beans to gain an understanding of how and when the mitochondrial genomes of the gene pools became distinct. The mtDNA of six wild bean accessions from Central and South America were digested with nine restriction endonucleases and analyzed by Southern hybridization. A total of twenty RFLPs were detected demonstrating a significantly higher amount of mtDNA variability in wild beans than in cultivated ones. All of the wild beans had the same mtDNA pattern for four out of the five inter-gene pool RFLPs, indicating that the polymorphism arose soon after domestication: two in the gene pool of the cultivated Mesoamerican beans and two in the gene pool of the cultivated Andean beans. The fifth RFLP must have occurred before domestication since the locus was also polymorphic in the wild beans. Wild beans from the south Andes were distinct and less variable than wild accessions of the north Andes and Mesoamerica. The distribution of mtDNA RFLPs among the wild beans supports the concept of two distinct domestication events for P. vulgaris. 相似文献
7.
María De La Fuente Ana M. González Antonio M. De Ron Marta Santalla 《Molecular breeding : new strategies in plant improvement》2013,31(3):501-516
Most studies on the genetic diversity of common bean (Phaseolus vulgaris L.) have focussed on accessions from the Mesoamerican gene pool compared to the Andean gene pool. A deeper knowledge of the genetic structure of Argentinian germplasm would enable researchers to determine how the Andean domestication event affected patterns of genetic diversity in domesticated beans and to identify candidates for genes targeted by selection during the evolution of the cultivated common bean. A collection of 116 wild and domesticated accessions representing the diversity of the Andean bean in Argentina was genotyped by means of 114 simple sequence repeat (SSR) markers. Forty-seven Mesoamerican bean accessions and 16 Andean bean accessions representing the diversity of Andean landraces and wild accessions were also included. Using the Bayesian algorithm implemented in the software STRUCTURE we identified five major groups that correspond to Mesoamerican and Argentinian wild accessions and landraces and a group that corresponds to accessions from different Andean and Mesoamerican countries. The neighbour-joining algorithm and principal coordinate clustering analysis confirmed the genetic relationships among accessions observed with the STRUCTURE analysis. Argentinian accessions showed a substantial genetic variation with a considerable number of unique haplotypes and private alleles, suggesting that they may have played an important role in the evolution of the species. The results of statistical analyses aimed at identifying genomic regions with consistent patterns of variation were significant for 35 loci (~20 % of the SSRs used in the Argentinian accessions). One of these loci mapped in or near the genomic region of the glutamate decarboxylase gene. Our data characterize the population structure of the Argentinian germplasm. This information on its diversity will be very valuable for use in introgressing Argentinian genes into commercial varieties because the majority of present-day common bean varieties are of Andean origin. 相似文献
8.
Structure of genetic diversity in the two major gene pools of common bean (Phaseolus vulgaris L., Fabaceae) 总被引:1,自引:0,他引:1
Myounghai Kwak Paul Gepts 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(5):979-992
Domesticated materials with well-known wild relatives provide an experimental system to reveal how human selection during
cultivation affects genetic composition and adaptation to novel environments. In this paper, our goal was to elucidate how
two geographically distinct domestication events modified the structure and level of genetic diversity in common bean. Specifically,
we analyzed the genome-wide genetic composition at 26, mostly unlinked microsatellite loci in 349 accessions of wild and domesticated
common bean from the Andean and Mesoamerican gene pools. Using a model-based approach, implemented in the software STRUCTURE,
we identified nine wild or domesticated populations in common bean, including four of Andean and four of Mesoamerican origins.
The ninth population was the putative wild ancestor of the species, which was classified as a Mesoamerican population. A neighbor-joining
analysis and a principal coordinate analysis confirmed genetic relationships among accessions and populations observed with
the STRUCTURE analysis. Geographic and genetic distances in wild populations were congruent with the exception of a few putative
hybrids identified in this study, suggesting a predominant effect of isolation by distance. Domesticated common bean populations
possessed lower genetic diversity, higher F
ST, and generally higher linkage disequilibrium (LD) than wild populations in both gene pools; their geographic distributions
were less correlated with genetic distance, probably reflecting seed-based gene flow after domestication. The LD was reduced
when analyzed in separate Andean and Mesoamerican germplasm samples. The Andean domesticated race Nueva Granada had the highest
F
ST value and widest geographic distribution compared to other domesticated races, suggesting a very recent origin or a selection
event, presumably associated with a determinate growth habit, which predominates in this race.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
9.
Anna Farina Valentina Rocchi Michela Janni Stefano Benedettelli Giulia De Lorenzo Renato D’Ovidio 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(7):1371-1379
Polygalacturonase-inhibiting proteins (PGIPs) are extracellular plant protein inhibitors of endo-polygalacturonases (PGs)
that belong to the leucine-rich repeat (LRR) protein family. In bean, PGIP is encoded by a small gene family of four members
among which Pvpgip2 encodes the most wide-spectrum and efficient inhibitor of fungal PGs. In order to evaluate the sequence polymorphism of Pvpgip2 and its functional significance, we have analyzed a number of wild and cultivated bean (P. vulgaris) accessions of Andean and Mesoamerican origin, and some genotypes from the related species P. coccineus, P. acutifolius, and P. lunatus. Our analyses indicate that the protein encoded by Pvpgip2 is highly conserved in the bean germplasm. The few detected polymorphic sites correspond to synonymous substitutions and
only two wild genotypes contain a Pvpgip2 with a single non-synonymous replacement. Sequence comparison showed a slightly larger variation in the related bean species
P. coccineus, P. acutifolius, and P. lunatus and confirmed the known phylogenetic relationships with P. vulgaris. The majority of the replacements were within the xxLxLxx region of the leucine rich repeat (LRR) domain and none of them
affected residues contributing to structural features. The variant PGIPs were expressed in Nicotiana benthamiana using PVX as vector and their inhibitory activity compared to that of PvPPGIP2. All the variants were able to fully inhibit
the four fungal PGs tested with minor differences. Taken together these results support the hypothesis that the overall sequence
conservation of PGIP2 and minor variation at specific sites is necessary for high-affinity recognition of different fungal
PGs.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
10.
Demographic factors shaped diversity in the two gene pools of wild common bean Phaseolus vulgaris L.
Wild common bean (Phaseolus vulgaris L.) is distributed throughout the Americas from Mexico to northern Argentina. Within this range, the species is divided into two gene pools (Andean and Middle American) along a latitudinal gradient. The diversity of 24 wild common bean genotypes from throughout the geographic range of the species was described by using sequence data from 13 loci. An isolation–migration model was evaluated using a coalescent analysis to estimate multiple demographic parameters. Using a Bayesian approach, Andean and Middle American subpopulations with high percentage of parentages were observed. Over all loci, the Middle American gene pool was more diverse than the Andean gene pool (πsil=0.0089 vs 0.0068). The two subpopulations were strongly genetically differentiated over all loci (Fst=0.29). It is estimated that the two current wild gene pools diverged from a common ancestor ∼111 000 years ago. Subsequently, each gene pool underwent a bottleneck immediately after divergence and lasted ∼40 000 years. The Middle American bottleneck population size was ∼46% of the ancestral population size, whereas the Andean was 26%. Continuous asymmetric gene flow was detected between the two gene pools with a larger number of migrants entering Middle American gene pool from the Andean gene pool. These results suggest that because of the complex population structure associated with the ancestral divergence, subsequent bottlenecks in each gene pool, gene pool-specific domestication and intense selection within each gene pool by breeders; association mapping would best be practised within each common bean gene pool. 相似文献
11.
Blair MW Giraldo MC Buendía HF Tovar E Duque MC Beebe SE 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,113(1):100-109
A diversity survey was used to estimate allelic diversity and heterozygosity of 129 microsatellite markers in a panel of 44 common bean (Phaseolus vulgaris L.) genotypes that have been used as parents of mapping populations. Two types of microsatellites were evaluated, based respectively on gene coding and genomic sequences. Genetic diversity was evaluated by estimating the polymorphism information content (PIC), as well as the distribution and range of alleles sizes. Gene-based microsatellites proved to be less polymorphic than genomic microsatellites in terms of both number of alleles (6.0 vs. 9.2) and PIC values (0.446 vs. 0.594) while greater size differences between the largest and the smallest allele were observed for the genomic microsatellites than for the gene-based microsatellites (31.4 vs. 19.1 bp). Markers that showed a high number of alleles were identified with a maximum of 28 alleles for the marker BMd1. The microsatellites were useful for distinguishing Andean and Mesoamerican genotypes, for uncovering the races within each genepool and for separating wild accessions from cultivars. Greater polymorphism and race structure was found within the Andean gene pool than within the Mesoamerican gene pool and polymorphism rate between genotypes was consistent with genepool and race identity. Comparisons between Andean genotypes had higher polymorphism (53.0%) on average than comparisons among Mesoamerican genotypes (33.4%). Within the Mesoamerican parental combinations, the intra-racial combinations between Mesoamerica and Durango or Jalisco race genotypes showed higher average rates of polymorphism (37.5%) than the within-race combinations between Mesoamerica race genotypes (31.7%). In multiple correspondance analysis we found two principal clusters of genotypes corresponding to the Mesoamerican and Andean gene pools and subgroups representing specific races especially for the Nueva Granada and Peru races of the Andean gene pool. Intra population diversity was higher within the Andean genepool than within the Mesoamerican genepool and this pattern was observed for both gene-based and genomic microsatellites. Furthermore, intra-population diversity within the Andean races (0.356 on average) was higher than within the Mesoamerican races (0.302). Within the Andean gene pool, race Peru had higher diversity compared to race Nueva Granada, while within the Mesoamerican gene pool, the races Durango, Guatemala and Jalisco had comparable levels of diversity which were below that of race Mesoamerica. 相似文献
12.
S. A. Angioi D. Rau G. Attene L. Nanni E. Bellucci G. Logozzo V. Negri P. L. Spagnoletti Zeuli R. Papa 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,121(5):829-843
This study focuses on the expansion of Phaseolus vulgaris in Europe. The pathways of distribution of beans into and across Europe were very complex, with several introductions from
the New World that were combined with direct exchanges between European and other Mediterranean countries. We have analyzed
here six chloroplast microsatellite (cpSSR) loci and two unlinked nuclear loci (for phaseolin types and Pv-shatterproof1). We have assessed the genetic structure and level of diversity of a large collection of European landraces of P. vulgaris (307) in comparison to 94 genotypes from the Americas that are representative of the Andean and Mesoamerican gene pools.
First, we show that most of the European common bean landraces (67%) are of Andean origin, and that there are no strong differences
across European regions for the proportions of the Andean and Mesoamerican gene pools. Moreover, cytoplasmic diversity is
evenly distributed across European regions. Secondly, the cytoplasmic bottleneck that was due to the introduction of P. vulgaris into the Old World was very weak or nearly absent. This is in contrast to evidence from nuclear analyses that have suggested
a bottleneck of greater intensity. Finally, we estimate that a relatively high proportion of the European bean germplasm (about
44%) was derived from hybridization between the Andean and Mesoamerican gene pools. Moreover, although hybrids are present
everywhere in Europe, they show an uneven distribution, with high frequencies in central Europe, and low frequencies in Spain
and Italy. On the basis of these data, we suggest that the entire European continent and not only some of the countries therein
can be regarded as a secondary diversification center for P. vulgaris. Finally, we outline the relevance of these inter-gene pool hybrids for plant breeding. 相似文献
13.
Background and Aims
The actual number of domestications of a crop is one of the key questions in domestication studies. Answers to this question have generally been based on relationships between wild progenitors and domesticated descendants determined with anonymous molecular markers. In this study, this question was investigated by determining the number of instances a domestication phenotype had been selected in a crop species. One of the traits that appeared during domestication of common bean (Phaseolus vulgaris) is determinacy, in which stems end with a terminal inflorescence. It has been shown earlier that a homologue of the arabidopsis TFL1 gene – PvTFL1y – controls determinacy in a naturally occurring variation of common bean.Methods
Sequence variation was analysed for PvTFL1y in a sample of 46 wild and domesticated accessions that included determinate and indeterminate accessions.Key Results
Indeterminate types – wild and domesticated – showed only synonymous nucleotide substitutions. Determinate types – observed only among domesticated accessions – showed, in addition to synonymous substitutions, non-synonymous substitutions, indels, a putative intron-splicing failure, a retrotransposon insertion and a deletion of the entire locus. The retrotransposon insertion was observed in 70 % of determinate cultivars, in the Americas and elsewhere. Other determinate mutants had a more restricted distribution in the Americas only, either in the Andean or in the Mesoamerican gene pool of common bean.Conclusions
Although each of the determinacy haplotypes probably does not represent distinct domestication events, they are consistent with the multiple (seven) domestication pattern in the genus Phaseolus. The predominance of determinacy in the Andean gene pool may reflect domestication of common bean prior to maize introduction in the Andes. 相似文献14.
Nanni L Bitocchi E Bellucci E Rossi M Rau D Attene G Gepts P Papa R 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(8):1341-1357
Evolutionary studies in plant and animal breeding are aimed at understanding the structure and organization of genetic variations
of species. We have identified and characterized a genomic sequence in Phaseolus vulgaris of 1,200 bp (PvSHP1) that is homologous to SHATTERPROOF-1 (SHP1), a gene involved in control of fruit shattering in Arabidopsis thaliana. The PvSHP1 fragment was mapped to chromosome Pv06 in P. vulgaris and is linked to the flower and seed color gene V. Amplification of the PvSHP1 sequence from the most agronomically important legume species showed a high degree of interspecies diversity in the introns
within the Phaseoleae, while the coding region was conserved across distant taxa. Sequencing of the PvSHP1 sequence in a sample of 91 wild and domesticated genotypes that span the geographic distribution of this species in the centers
of origin showed that PvSHP1 is highly polymorphic and, therefore, particularly useful to further investigate the origin and domestication history of
P. vulgaris. Our data confirm the gene pool structure seen in P. vulgaris along with independent domestication processes in the Andes and Mesoamerica; they provide additional evidence for a single
domestication event in Mesoamerica. Moreover, our results support the Mesoamerican origin of this species. Finally, we have
developed three indel-spanning markers that will be very useful for bean germplasm characterization, and particularly to trace
the distribution of the domesticated Andean and Mesoamerican gene pools. 相似文献
15.
Paul Gepts 《Economic botany》1990,44(3):28-38
The genusPhaseolus (Fabaceae) consists of some 50 species, all of which are distributed in the Americas. Four of these contain cultigens.P. vulgaris (common bean),P. lunatus (lima bean),P. acutifolius (tepary bean),P. coccineus subsp.coccineus (runner bean); andP. coccineus subsp.polyanthus (no English vernacular name). Biochemical markers—phaseolin seed storage protein and isozymes—have provided new evidence on the organization of the first three species. Domestication has possibly caused a strong reduction in genetic diversity inP. vulgaris andP. acutifolius. BothP. vulgaris andP. lunatus cultivars result from at least two independent domestications, in Mesoamerica and in the Andes. These two species consist of two gene pools, each of which includes wild ancestors and their respective cultivated descendants. Our findings suggest the need for additional emphasis on genetic conservation of wild ancestors and their use in breeding programs and for a comparison of inter-gene pool vs. intra-gene pool crosses in breeding programs. 相似文献
16.
Response of predatory mites with different rearing histories to volatiles of uninfested plants 总被引:9,自引:0,他引:9
The behavioural response of the predatory mite Phytoseiulus persimilis to volatiles from several host plants of its prey, spider mites in the genus Tetranychus, was investigated in a Y-tube olfactometer. A positive response to volatiles from tomato leaves and Lima bean leaves was recorded, whereas no response was observed to volatiles from cucumber leaves, or leaves of Solanum luteum and Solanum dulcamara.Different results were obtained for predators that differed in rearing history. Predators that were reared on spider mites (Tetranychus urticae) on Lima bean leaves did respond to volatiles from Lima bean leaves, while predators that had been reared on the same spider mite species but with cucumber as host plant did not respond to Lima bean leaf volatiles. This effect is compared with the effect of rearing history on the response of P. persimilis to volatile allelochemicals of prey-infested plant leaves. 相似文献
17.
Sample size for collecting seeds in germplasm conservation: the case of the Lima bean (Phaseolus lunatus L.) 总被引:2,自引:0,他引:2
I. Zoro Bi A. Maquet J. Degreef B. Wathelet J. P. Baudoin 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(1-2):187-194
The design of optimum sampling strategies integrating criteria of efficiency relevant to multilocus models and many target
populations has been investigated with respect to the number of plants and the number of seeds per plant to be sampled for
a Lima bean (Phaseolus lunatus L.) gene pool. This study, using five populations and six polymorphic enzyme loci, shows that the number of plants rather
than the number of seeds collected per plant primarily determines the success of seed sampling, suggesting that plant number
plays an essential part in maintaining the allelic multiplicity of predominantly selfing species like Lima bean. According
to the results, it appears that among Lima bean populations an efficient sampling procedure is achieved by collecting 1–4
seeds from 200 to 300 plants. These sample sizes will retain 8–10 alleles, regardless of their frequencies. When we consider
polymorphism at the 5% level, it is expected that sampling 10–80 plants will collect combinations of 4–8 alleles. Based on
data from genetic and demographic studies, we suggest an efficient sampling scheme for Lima bean germplasm at both population
and geographical levels.
Received: 10 March 1998 / Accepted: 1 April 1998 相似文献
18.
The common bean (Phaseolus vulgaris) is one of the most important crop plants. About 50% of its genome is composed of repetitive sequences, but only a little
fraction was isolated and characterized so far. In this paper, a new repetitive DNA family from the species, named PvMeso, was isolated and characterized in both gene pools of P. vulgaris (Andean and Mesoamerican) and related species. Two fragments, 1.7 and 2.3 kb long, were cloned from BAC 255F18, which has
previously shown a repetitive pattern. The subclone PvMeso-31 showed a terminal block in chromosome 7. This subclone contains a 1,705 bp long, AT-rich repeat with small internal
repeats and shares a 1.2 kb region with PvMeso-47, derived from the 2.3 kb fragment. The presence of this repetitive block was restricted to Mesoamerican accessions
of the common bean. In P. acutifolius, P. leptostachyus and Andean P. vulgaris, only a faint, 2.3 kb fragment was visualized in Southern experiments. Moreover, in Mesoamerican accessions, two other fragments
(1.7 kb and 3.4 kb) were strongly labelled as well. Taken together, our results indicate that PvMeso is a recently emerged, repeat family initially duplicated in chromosome 11, on ancestral Mesoamerican accession, and
later amplified in chromosome 7, after the split of the two major gene pools of the common bean. 相似文献
19.
Pedrosa-Harand A de Almeida CC Mosiolek M Blair MW Schweizer D Guerra M 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(5):924-933
The extent of 5S and 45S ribosomal DNA (rDNA) variation was investigated in wild and domesticated common beans (Phaseolus vulgaris) chosen to represent the known genetic diversity of the species. 5S and 45S rDNA probes were localized on mitotic chromosomes
of 37 accessions by fluorescent in situ hybridization (FISH). The two 5S rDNA loci were largely conserved within the species,
whereas a high variation in the number of 45S rDNA loci and changes in position of loci and number of repeats per locus were
observed. Domesticated accessions from the Mesoamerican gene pool frequently had three 45S rDNA loci per haploid genome, and
rarely four. Domesticated accessions from Andean gene pool, particularly from the race Peru, showed six, seven, eight or nine
loci, but seven loci were found in all three races of this gene pool. Between three and eight loci were observed in accessions
resulting from crosses between Andean and Mesoamerican genotypes. The presence of two to eight 45S rDNA loci in wild common
beans from different geographic locations indicates that the 45S rDNA amplification observed in the Andean lineage took place
before domestication. Our data suggest that ectopic recombination between terminal chromosomal regions might be the mechanism
responsible for this variation. 相似文献
20.
I. N. Wagara A. W. Mwang'ombe J. W. Kimenju R. A. Buruchara R. Jamnadass P. A. O. Majiwa 《Journal of Phytopathology》2004,152(4):235-242
Genetic diversity of 50 Phaeoisariopsis griseola isolates collected from different agroecological zones in Kenya was studied using group‐specific primers and amplified fragment length polymorphism (AFLP) markers. Group‐specific primers differentiated the isolates into Andean and Mesoamerican groups, corresponding to the two common‐bean gene pools. Significant polymorphisms were observed with all the AFLP primer combinations used, reflecting a wide genetic diversity in the P. griseola population. A total of 207 fingerprints was generated, of which 178 were polymorphic. Cluster analysis of the polymorphic bands also separated the isolates into the two groups defined by group‐specific primers. All the isolates examined were grouped into three virulence populations; Andean, Afro‐Andean and Mesoamerican, and their genetic diversity measured. On average, greater diversity (91%) was detected within populations than between populations (9%). The genetic distance between Andean and Mesoamerican populations was higher (D = 0.0269) than between Andean and Afro‐Andean (D = 0.0095). The wide genetic diversity reported here has significant implications in breeding for resistance to angular leaf spot and should be taken into consideration when screening and deploying resistant bean genotypes. 相似文献