共查询到20条相似文献,搜索用时 31 毫秒
1.
Two new species of Fulvifomes are described from specimens collected in rainforests of Nonggang Nature Reserve of southern China, based on morphological characteristics and molecular phylogenetic analysis of the internal transcribed spacer (ITS) and nuclear large subunit ribosomal DNA (nLSU) sequences. Fulvifomes nonggangensis sp. nov. is characterized by perennial, sessile and solitary basidiocarps, applanate pileus, small cystidioles of 9.9–15.4 × 2.9–3.5 μm, large pores of 5–6 per mm, a dimitic hyphal system, and broadly ellipsoid basidiospores of 4.3–5.3 × 3.3–4.2 μm. F. tubogeneratus sp. nov. is characterized by perennial, sessile, and imbricate basidiocarps, a duplex context, small pores of 7–8 per mm, a dimitic hyphal system, and ovoid to subglobose basidiospores of 5.72 × 5.00 μm. 相似文献
2.
Meng Jiao Cui Xin Wei Peng Liang Xia Ji Ping Yi Zhi He Yu Jian Xin Deng Qi Li Li 《Mycobiology》2021,49(3):267
Two Diaporthe species isolated from fruit of Citrus sinensis in China were characterized based on morphology and multilocus phylogeny of ITS, tef1, and tub2 gene sequences. The phylogeny indicated that the two species match Diaporthe taoicola and D. siamensis. A critical examination of phenotypic characteristics confirmed the phylogenetic results. Diaporthe taoicola was morphologically characterized by producing Alpha conidia with tapering toward both ends. Meanwhile, D. siamensis produced cylindrical or ellipsoidal Alpha conidia with two oil drops. Pathogenicity tests revealed that both species were pathogenic to fruit of C. sinensis. To our knowledge, the two species were firstly reported on Citrus sinensis in China. 相似文献
3.
4.
Two new parthenogenetic species of Longidorus were found in Arkansas. Longidorus grandis n. sp. is characterized by its body (5.80-8.24 mm), slightly offset head, head width 20-27 µm, odontostyle 86-100 µm, guide ring 26-35 µm posterior to the anterior end, short conoid to mammiliform tail. Longidorus grandis n. sp. is similar to L. vineacola Sturhan &Weischer, 1964; L. lusitanicus Macara, 1985; L. edmundsi Hunt &Siddiqi, 1977; L. kuiperi Brinkman, Loof &Barbez, 1987; L. balticus Brzeski, Peneva &Brown, 2000; L. closelongatus Stoyanov, 1964; and L. seinhorsti Peneva, Loof &Brown, 1998. Longidorus paralongicaudatus n. sp. is characterized by its body length (2.60-5.00 µm), anteriorly flattened and offset head region 13-18 µm wide, odontostyle length 92-127 µm, guide ring 21-30 µm posterior to the anterior end, tail elongate-conical, and c'' = 1.2-2.6. Longidorus paralongicaudatus n. sp. most closely resembles L. longicaudatus Siddiqi, 1962; L. socialis Singh &Khan, 1996; L. juvenilis Dalmasso, 1969; and L. curvatus Khan, 1986. 相似文献
5.
Vipul Solanki Karen Krüger Conor J. Crawford Alonso Pardo-Vargas Jos Danglad-Flores Kim Le Mai Hoang Leeann Klassen D. Wade Abbott Peter H. Seeberger Rudolf I. Amann Hanno Teeling Jan-Hendrik Hehemann 《The ISME journal》2022,16(7):1818
Microbial glycan degradation is essential to global carbon cycling. The marine bacterium Salegentibacter sp. Hel_I_6 (Bacteroidota) isolated from seawater off Helgoland island (North Sea) contains an α-mannan inducible gene cluster with a GH76 family endo-α-1,6-mannanase (ShGH76). This cluster is related to genetic loci employed by human gut bacteria to digest fungal α-mannan. Metagenomes from the Hel_I_6 isolation site revealed increasing GH76 gene frequencies in free-living bacteria during microalgae blooms, suggesting degradation of α-1,6-mannans from fungi. Recombinant ShGH76 protein activity assays with yeast α-mannan and synthetic oligomannans showed endo-α-1,6-mannanase activity. Resolved structures of apo-ShGH76 (2.0 Å) and of mutants co-crystalized with fungal mannan-mimicking α-1,6-mannotetrose (1.90 Å) and α-1,6-mannotriose (1.47 Å) retained the canonical (α/α)6 fold, despite low identities with sequences of known GH76 structures (GH76s from gut bacteria: <27%). The apo-form active site differed from those known from gut bacteria, and co-crystallizations revealed a kinked oligomannan conformation. Co-crystallizations also revealed precise molecular-scale interactions of ShGH76 with fungal mannan-mimicking oligomannans, indicating adaptation to this particular type of substrate. Our data hence suggest presence of yet unknown fungal α-1,6-mannans in marine ecosystems, in particular during microalgal blooms.Subject terms: Metagenomics, Microbial ecology, Structural biology, Fungal ecology, Molecular ecology 相似文献
6.
Longidorus paravineacola n. sp., described herein, was found in a survey of longidorids of Arkansas. It is a parthenogeneticspecies characterized by its long body (6.68-9.85 mm); slightly expanded and rounded head, head width 21-27 µm; odontostyle length 95-114 µm; guide ring 28-37 µm posterior to the head end; short rounded tail, and c'' = 0.6-1.0. Longidorus paravineacola n. sp. is similar to the amphimictic species L. vineacola Sturhan &Weischer, 1964; L. balticus Brzeski, Peneva &Brown, 2000; L. kuiperi Brinkman, Loof &Barbez, 1987; and parthenogenetic species L. crassus Thorne, 1974, which also occurred in the type locality. 相似文献
7.
Recent discoveries reveal that southern China’s karsts hold the most diverse and morphologically modified subterranean trechine beetles in the world, albeit the first troglobitic blind beetle was only reported in the early 1990’s. In total, 110 species belonging to 43 genera of cavernicolous trechines have hitherto been recorded from the karsts of southern China, including the following five new genera proposed below: Shiqianaphaenops Tian, gen. n., to contain two species: Shiqianaphaenops
majusculus (Uéno, 1999) (= Shenaphaenops
majusculus Uéno, 1999, comb. n.), the type species from Cave Feng Dong, Shiqian, Guizhou, and Shiqianaphaenops
cursor (Uéno, 1999) (= Shenaphaenops
cursor Uéno, 1999, comb. n.), from Cave Shenxian Dong, Shiqian, Guizhou; and the monotypic Dianotrechus Tian, gen. n. (the type species: Dianotrechus
gueorguievi Tian, sp. n., from Cave Dashi Dong, Kunming, Yunnan), Tianeotrechus Tian & Tang, gen. n. (the type species: Tianeotrechus
trisetosus Tian & Tang, sp. n., from Cave Bahao Dong, Tian’e County, Guangxi), Huoyanodytes Tian & Huang, gen. n. (the type species: Huoyanodytes
tujiaphilus Tian & Huang, sp. n., from Longshan, Hunan) and Wanhuaphaenops Tian & Wang, gen. n. (the type species: Wanhuaphaenops
zhangi Tian & Wang, sp. n., from Cave Songjia Dong, Chenzhou, Hunan). 相似文献
8.
Bin Yan Christopher H. Dietrich Xiaofei Yu Meng Jiao Renhuai Dai Maofa Yang 《Ecology and evolution》2022,12(6)
The subfamily Typhlocybinae is a ubiquitous, highly diverse group of mostly tiny, delicate leafhoppers. The tribal classification has long been controversial and phylogenetic methods have only recently begun to test the phylogenetic status and relationships of tribes. To shed light on the evolution of Typhlocybinae, we performed phylogenetic analyses based on 28 newly sequenced and 19 previously sequenced mitochondrial genomes representing all currently recognized tribes. The results support the monophyly of the subfamily and its sister‐group relationship to Mileewinae. The tribe Zyginellini is polyphyletic with some included genera derived independently within Typhlocybini. Ancestral character state reconstruction suggests that some morphological characters traditionally considered important for diagnosing tribes (presence/absence of ocelli, development of hind wing submarginal vein) are homoplastic. Divergence time estimates indicate that the subfamily arose during the Middle Cretaceous and that the extant tribes arose during the Late Cretaceous. Phylogenetic results support establishment of a new genus, Subtilissimia Yan & Yang gen. nov., with two new species, Subtilissimia fulva Yan & Yang sp. nov. and Subtilissimia pellicula Yan & Yang sp. nov.; but indicate that two previously recognized species of Farynala distinguished only by the direction of curvature of the processes of the aedeagus are synonyms, that is, Farynala dextra Yan & Yang, 2017 equals Farynala sinistra Yan & Yang, 2017 syn. nov. A key to tribes of Typhlocybinae is provided. 相似文献
9.
Louise A O'Sullivan Erwan G Roussel Andrew J Weightman Gordon Webster Casey RJ Hubert Emma Bell Ian Head Henrik Sass R John Parkes 《The ISME journal》2015,9(4):922-933
Bacterial spores are widespread in marine sediments, including those of thermophilic, sulphate-reducing bacteria, which have a high minimum growth temperature making it unlikely that they grow in situ. These Desulfotomaculum spp. are thought to be from hot environments and are distributed by ocean currents. Their cells and spores upper temperature limit for survival is unknown, as is whether they can survive repeated high-temperature exposure that might occur in hydrothermal systems. This was investigated by incubating estuarine sediments significantly above (40–80 °C) maximum in situ temperatures (∼23 °C), and with and without prior triple autoclaving. Sulphate reduction occurred at 40–60 °C and at 60 °C was unaffected by autoclaving. Desulfotomaculum sp. C1A60 was isolated and was most closely related to the thermophilic D. kuznetsoviiT (∼96% 16S rRNA gene sequence identity). Cultures of Desulfotomaculum sp. C1A60, D. kuznetsoviiTand D. geothermicum B2T survived triple autoclaving while other related Desulfotomaculum spp. did not, although they did survive pasteurisation. Desulfotomaculum sp. C1A60 and D. kuznetsovii cultures also survived more extreme autoclaving (C1A60, 130 °C for 15 min; D. kuznetsovii, 135 °C for 15 min, maximum of 154 °C reached) and high-temperature conditions in an oil bath (C1A60, 130° for 30 min, D. kuznetsovii 140 °C for 15 min). Desulfotomaculum sp. C1A60 with either spores or predominantly vegetative cells demonstrated that surviving triple autoclaving was due to spores. Spores also had very high culturability compared with vegetative cells (∼30 × higher). Combined extreme temperature survival and high culturability of some thermophilic Desulfotomaculum spp. make them very effective colonisers of hot environments, which is consistent with their presence in subsurface geothermal waters and petroleum reservoirs. 相似文献
10.
The Chinese species of the silphaeformis group of the genus Tachinus Gravenhorst are revised with fifteen species being treated. Thirteen of them are described as new: T. armatus Feng & Li, sp. n. (Sichuan), T. cavazzutii Feng, Li & Schülke, sp. n. (Sichuan), T. coronatus Feng, Li & Schülke, sp. n. (Ningxia, Qinghai), T. hercules Feng, Li & Schülke, sp. n. (Sichuan), T. hujiayaoi Feng, Li & Schülke, sp. n. (Shaanxi), T. jiuzhaigouensis Feng, Li & Schülke, sp. n. (Sichuan), T. linzhiensis Feng & Li, sp. n. (Tibet), T. maderianus Feng & Li, sp. n. (Sichuan), T. mengdaensis Feng, Li & Schülke, sp. n. (Qinghai), T. oblongoelytratus Feng & Li, sp. n. (Sichuan), T. parahercules Feng, Li & Schülke, sp. n. (Sichuan), T. paralinzhiensis Feng & Li, sp. n. (Tibet), and T. yini Feng, Li & Schülke, sp. n. (Sichuan). The two known species are redescribed based on the holotypes and additional material. Illustrations of the habitus and major diagnostic characters, distributional maps, and identification keys of all species are included. 相似文献
11.
Paurodontella parapitica n. sp., collected from the rhizosphere of an apple tree in Kermanshah province, western Iran, is described. The new species is characterized by a body length of 505 to 723 µm (females) and 480 to 600 µm (males), lip region continuous by depression; 7 to 8 μm broad, 3 to 4 µm high, stylet length 7 to 9 µm or 1 to 1.3 times the lip region diameter, short postuterine sac of 4 to 6 μm long, lateral fields with five to six incisures; outer incisures crenated and inner incisures weakly crenated, excretory pore situated 90 to 100 µm from anterior end; functional males common in the population, with spicules 24 to 26 μm long. Tail of both sexes similar, almost straight and elongate-conoid. The new species resembles in morphology and morphometrics to four known species of the genus, namely P. apitica, P. minuta, P. myceliophaga, and P. sohailai. The results of phylogenetic analyses based on sequences of D2/D3 expansion region of 28S rRNA gene revealed this genus is polyphyletic in four different clades in Tylenchid. 相似文献
12.
Michelle M Gehringer Lewis Adler Alexandra A Roberts Michelle C Moffitt Troco K Mihali Toby J T Mills Claus Fieker Brett A Neilan 《The ISME journal》2012,6(10):1834-1847
The nitrogen-fixing bacterium, Nostoc, is a commonly occurring cyanobacterium often found in symbiotic associations. We investigated the potential of cycad cyanobacterial endosymbionts to synthesize microcystin/nodularin. Endosymbiont DNA was screened for the aminotransferase domain of the toxin biosynthesis gene clusters. Five endosymbionts carrying the gene were screened for bioactivity. Extracts of two isolates inhibited protein phosphatase 2A and were further analyzed using electrospray ionization mass spectrometry (ESI-MS)/MS. Nostoc sp. ‘Macrozamia riedlei 65.1'' and Nostoc sp. ‘Macrozamia serpentina 73.1'' both contained nodularin. High performance liquid chromatography (HPLC) HESI-MS/MS analysis confirmed the presence of nodularin at 9.55±2.4 ng μg−1 chlorophyll a in Nostoc sp. ‘Macrozamia riedlei 65.1'' and 12.5±8.4 ng μg−1 Chl a in Nostoc sp. ‘Macrozamia serpentina 73.1'' extracts. Further scans indicated the presence of the rare isoform [L-Har2] nodularin, which contains ℒ-homoarginine instead of ℒ-arginine. Nodularin was also present at 1.34±0.74 ng ml−1 (approximately 3 pmol per g plant ww) in the methanol root extracts of M. riedlei MZ65, while the presence of [L-Har2] nodularin in the roots of M. serpentina MZ73 was suggested by HPLC HESI-MS/MS analysis. The ndaA-B and ndaF genomic regions were sequenced to confirm the presence of the hybrid polyketide/non-ribosomal gene cluster. A seven amino-acid insertion into the NdaA-C1 domain of N. spumigena NSOR10 protein was observed in all endosymbiont-derived sequences, suggesting the transfer of the nda cluster from N. spumigena to terrestrial Nostoc species. This study demonstrates the synthesis of nodularin and [L-Har2] nodularin in a non-Nodularia species and the production of cyanobacterial hepatotoxin by a symbiont in planta. 相似文献
13.
Nguyen Phuong Thao Bui Thi Thuy Luyen Jung Eun Koo Sohyun Kim Young Sang Koh Nguyen Xuan Cuong Nguyen Hoai Nam Phan Van Kiem Young Ho Kim Chau Van Minh 《Biological research》2015,48(1)
Background
In the present study, we examined the inhibitory effects of a methanolic extract, dichloromethane fraction, water layer, and polyhydroxylated sterols (1–4) isolated from the Vietnamese starfish Protoreaster nodosus on pro-inflammatory cytokine (IL-12 p40, IL-6, and TNF-α) production in LPS-stimulated bone marrow-derived dendritic cells (BMDCs) using enzyme-linked immunosorbent assays (ELISA).Results
The methanolic extract and dichloromethane fraction exerted potent inhibitory effects on the production of all three pro-inflammatory cytokines, with IC50 values ranging from 0.60 ± 0.01 to 26.19 ± 0.64 μg/mL. Four highly pure steroid derivatives (1–4) were isolated from the dichloromethane fraction and water layer of P. nodosus. Potent inhibitory activities were also observed for (25S) 5α-cholestane-3β,4β,6α,7α,8β,15α,16β,26-octol (3) on the production of IL-12 p40 and IL-6 (IC50s = 3.11 ± 0.08 and 1.35 ± 0.03 μM), and for (25S) 5α-cholestane-3β,6α,8β,15α,16β,26-hexol (1) and (25S) 5α-cholestane-3β,6α,7α,8β,15α,16β,26-heptol (2) on the production of IL-12 p40 (IC50s = 0.01 ± 0.00 and 1.02 ± 0.01 μM). Moreover, nodososide (4) exhibited moderate inhibitory effects on IL-12 p40 and IL-6 production.Conclusion
This is the first report of the anti-inflammatory activity from the starfish P. nodosus. The main finding of this study is the identification oxygenated steroid derivatives from P. nodosus with potent anti-inflammatory activities that may be developed as therapeutic agents for inflammatory diseases. 相似文献14.
Two new species of the empoascine leafhopper genus Membranacea Qin & Zhang are reported from China: Membranacea hubeiensis Yu & Yang, sp. n. and Membranacea stenoprocessa Yu & Yang, sp. n.. A key to distinguish all species of the genus is provided. 相似文献
15.
Bees (Anthophila) are one of the major groups of angiosperm-pollinating insects and accordingly are widely studied in both basic and applied research, for which it is essential to have a clear understanding of their phylogeny, and evolutionary history. Direct evidence of bee evolutionary history has been hindered by a dearth of available fossils needed to determine the timing and tempo of their diversification, as well as episodes of extinction. Here we describe four new compression fossils of bees from three different deposits (Miocene of la Cerdanya, Spain; Oligocene of Céreste, France; and Eocene of the Green River Formation, U.S.A.). We assess the similarity of the forewing shape of the new fossils with extant and fossil taxa using geometric morphometrics analyses. Predictive discriminant analyses show that three fossils share similar forewing shapes with the Apidae [one of uncertain tribal placement and perhaps near Euglossini, one definitive bumble bee (Bombini), and one digger bee (Anthophorini)], while one fossil is more similar to the Andrenidae. The corbiculate fossils are described as Euglossopteryx biesmeijeri De Meulemeester, Michez, & Engel, gen. nov. sp. nov. (type species of Euglossopteryx Dehon & Engel, n. gen.) and Bombus cerdanyensis Dehon, De Meulemeester, & Engel, sp. nov. They provide new information on the distribution and timing of particular corbiculate groups, most notably the extension into North America of possible Eocene-Oligocene cooling-induced extinctions. Protohabropoda pauli De Meulemeester & Michez, gen. nov. sp. nov. (type species of Protohabropoda Dehon & Engel, n. gen.) reinforces previous hypotheses of anthophorine evolution in terms of ecological shifts by the Oligocene from tropical to mesic or xeric habitats. Lastly, a new fossil of the Andreninae, Andrena antoinei Michez & De Meulemeester, sp. nov., further documents the presence of the today widespread genus Andrena Fabricius in the Late Oligocene of France. 相似文献
16.
Peng-Fei Zheng Zhuang Xiong Cui-ying Liao Xin Zhang Mei Feng Xiao-Zheng Wu Jing Lin Lin-Sheng Lei You-Cheng Zhang Shao-Hua Wang Xue-Tao Xu 《Journal of enzyme inhibition and medicinal chemistry》2021,36(1):1938
In this paper, bis (indol-3-yl) methanes (BIMs) were synthesised and evaluated for their inhibitory activity against α-glucosidase and α-amylase. All synthesised compounds showed potential α-glucosidase and α-amylase inhibitory activities. Compounds 5 g (IC50: 7.54 ± 1.10 μM), 5e (IC50: 9.00 ± 0.97 μM), and 5 h (IC50: 9.57 ± 0.62 μM) presented strongest inhibitory activities against α-glucosidase, that were ∼ 30 times stronger than acarbose. Compounds 5 g (IC50: 32.18 ± 1.66 µM), 5 h (IC50: 31.47 ± 1.42 µM), and 5 s (IC50: 30.91 ± 0.86 µM) showed strongest inhibitory activities towards α-amylase, ∼ 2.5 times stronger than acarbose. The mechanisms and docking simulation of the compounds were also studied. Compounds 5 g and 5 h exhibited bifunctional inhibitory activity against these two enzymes. Furthermore, compounds showed no toxicity against 3T3-L1 cells and HepG2 cells.
Highlights
- A series of bis (indol-3-yl) methanes (BIMs) were synthesised and evaluated inhibitory activities against α-glucosidase and α-amylase.
- Compound 5g exhibited promising activity (IC50 = 7.54 ± 1.10 μM) against α-glucosidase.
- Compound 5s exhibited promising activity (IC50 = 30.91 ± 0.86 μM) against α-amylase.
- In silico studies were performed to confirm the binding interactions of synthetic compounds with the enzyme active site.
17.
Dawoon Chung Yong Min Kwon Ji Yeon Lim Seung Sub Bae Grace Choi Dae-Sung Lee 《Mycobiology》2022,50(4):244
Trichoderma fungi have been intensively studied for mycoparasitism, and the latter is closely related to their cell-wall degrading enzymes including chitinase. Here, we studied marine-derived Trichoderma spp., isolated from distinct sources and locations, for chitinolytic and antifungal activity. Based on morphological and phylogenetic analyses, two strains designated GJ-Sp1 and TOP-Co8 (isolated from a marine sponge and a marine alga, respectively) were identified as Trichoderma bissettii. This species has recently been identified as a closely related species to Trichoderma longibrachiatum. The extracellular crude enzymes of GJ-Sp1 and TOP-Co8 showed activities of chitobiosidase and β-N-acetylglucosaminidase (exochitinase) and chitotriosidase (endochitinase). The optimum chitinolytic activity of the crude enzymes was observed at 50 °C, pH 5.0, 0–0.5% NaCl concentrations, and the activities were stable at temperatures ranging from 10 to 40 °C for 2 h. Moreover, the crude enzymes showed inhibitory activity against hyphal growth of two filamentous fungi Aspergillus flavus and Aspergillus niger. To the best of our knowledge, this is the first report of the chitinolytic and antifungal activity of T. bissettii. 相似文献
18.
This synopsis provides an identification key to the genera of Tribe Lachnophorini of the Western and Eastern Hemispheres including five genera previously misplaced in carabid classifications. The genus Asklepia Liebke, 1938 is revised with 23 new species added and four species reassigned from Eucaerus LeConte, 1853 to Asklepia Liebke, 1938. In addition, a new genus is added herein to the Tribe: Peruphorticus
gen. n. with its type species P. gulliveri
sp. n. from Perú. Five taxa previously assigned to other tribes have adult attributes that make them candidates for classification in the Lachnophorini: Homethes Newman, Aeolodermus Andrewes, Stenocheila Laporte de Castelnau, Diplacanthogaster Liebke, and Selina Motschulsky are now considered to belong to the Lachnophorini as genera incertae sedis. Three higher level groups are proposed to contain the 18 recognized genera: the Lachnophorina, Eucaerina, and incertae sedis.Twenty-three new species of the genus Asklepia are described and four new combinations are presented. They are listed with their type localities as follows: (geminata species group)
Asklepia geminata (Bates, 1871), comb. n, Santarém, Rio Tapajós, Brazil; (hilaris species group)
Asklepia campbellorum Zamorano & Erwin, sp. n., 20 km SW Manaus, Brazil, Asklepia demiti Erwin & Zamorano, sp. n., circa Rio Demiti, Brazil, Asklepia duofos Zamorano & Erwin, sp. n., 20 km SW Manaus, Brazil, Asklepia hilaris (Bates, 1871), comb. n, São Paulo de Olivença, Brazil, Asklepia grammechrysea Zamorano & Erwin, sp. n., circa Pithecia, Cocha Shinguito, Perú, Asklepia lebioides (Bates, 1871), comb. n, Santarém, Rio Tapajós, Brazil, Asklepia laetitia Zamorano & Erwin, sp. n., Leticia, Colombia, Asklepia matomena Zamorano & Erwin, sp.n., 20 km SW Manaus, Brazil; (pulchripennis species group)
Asklepia adisi Erwin & Zamorano, sp. n., Ilha de Marchantaria, Lago Camaleão, Brazil, Asklepia asuncionensis Erwin & Zamorano, sp. n., Asunción, Río Paraguay, Paraguay, Asklepia biolat Erwin & Zamorano, sp. n., BIOLAT Biological Station, Pakitza, Perú, Asklepia bracheia Zamorano & Erwin, sp. n., circa Explornapo Camp, Río Napo, Cocha Shimagai, Perú, Asklepia cuiabaensis Erwin & Zamorano, sp. n., Cuiabá, Brazil, Asklepia ecuadoriana Erwin & Zamorano, sp. n., Limoncocha, Ecuador, Asklepia kathleenae Erwin & Zamorano, sp. n., Belém, Brazil, Asklepia macrops Erwin & Zamorano, sp. n., Concordia, Río Uruguay, Argentina, Asklepia marchantaria Erwin & Zamorano, sp. n., Ilha de Marchantaria, Lago Camaleão, Brazil, Asklepia marituba Zamorano & Erwin, sp. n., Marituba, Ananindeua, Brazil, Asklepia paraguayensis Zamorano & Erwin, sp. n., San Lorenzo, Rio Paraguay, Paraguay, Asklepia pakitza Erwin & Zamorano, sp. n., BIOLAT Biological Station, Pakitza, Perú, Asklepia pulchripennis (Bates, 1871), comb. n, Santarém, Rio Tapajós, Brazil, Asklepia samiriaensis Zamorano & Erwin, sp. n., Boca del Río Samiria, Perú, Asklepia stalametlitos Zamorano & Erwin, sp. n., Guayamer, Río Mamoré, Bolivia, Asklepia strandi Liebke, 1938, Guyana, Asklepia surinamensis Zamorano & Erwin, sp. n., l’Hermitage, Surinam River, Surinam, Asklepia vigilante Erwin & Zamorano, sp. n., Boca del Río Samiria, Perú. Images of adults of all 18 genera are provided. 相似文献
19.
Background
Rumex species are traditionally used for the treatment of neurological disorders including headache, migraine, depression, paralysis etc. Several species have been scientifically validated for antioxidant and anticholinestrase potentials. This study aims to investigate Rumex hastatus D. Don crude methanolic extract, subsequent fractions, saponins and flavonoids for acetylcholinestrase, butyrylcholinestrase inhibition and diverse antioxidant activities to validate its folkloric uses in neurological disorders. Rumex hastatus crude methanolic extract (Rh. Cr), subsequent fractions; n-hexane (Rh. Hex), chloroform (Rh. Chf), ethyl acetate (Rh. EtAc), aqueous fraction (Rh. Aq), crude saponins (Rh. Sp) and flavonoids (Rh. Fl) were investigated against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) at various concentrations (125, 250, 500, 1000 μg/mL) using Ellman’s spectrophotometric analysis. Antioxidant potentials of Rh. Sp and Rh. Fl were evaluated using DPPH, H2O2 and ABTS free radical scavenging assays at 62.5, 125, 250, 500, 1000 μg/mL.Results
All the test samples showed concentration dependent cholinesterase inhibition and radicals scavenging activity. The AChE inhibition potential of Rh. Sp and Rh. Fl were most prominent i.e., 81.67 ± 0.88 and 91.62 ± 1.67 at highest concentration with IC50 135 and 20 μg/mL respectively. All the subsequent fractions exhibited moderate to high AChE inhibition i.e., Rh. Cr, Rh. Hex, Rh. Chf, Rh. EtAc and Rh. Aq showed IC50 218, 1420, 75, 115 and 1210 μg/mL respectively. Similarly, against BChE various plant extracts i.e., Rh. Sp, Rh. Fl, Rh. Cr, Rh. Hex, Rh. Chf, Rh. EtAc and Rh. Aq resulted IC50 165, 175, 265, 890, 92, 115 and 220 μg/mL respectively. In DPPH free radical scavenging assay, Rh. Sp and Rh. Fl showed comparable results with the positive control i.e., 63.34 ± 0.98 and 76.93 ± 1.13% scavenging at 1 mg/mL concentration (IC50 312 and 104 μg/mL) respectively. The percent ABTS radical scavenging potential exhibited by Rh. Sp and Rh. Fl (1000 μg/mL) were 82.58 ± 0.52 and 88.25 ± 0.67 with IC50 18 and 9 μg/mL respectively. Similarly in H2O2 scavenging assay, the Rh. Sp and Rh. Fl exhibited IC50 175 and 275 μg/mL respectively.Conclusion
The strong anticholinesterase and antioxidant activities of Rh. Sp, Rh. Fl and various fractions of R. hastatus support the purported ethnomedicinal uses and recommend R. hastatus as a possible remedy for the treatment of AD and neurodegenerative disorders. 相似文献20.
Zul Kamal Farhat Ullah Muhammad Ayaz Abdul Sadiq Sajjad Ahmad Anwar Zeb Abid Hussain Muhammad Imran 《Biological research》2015,48(1)