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1.
Kenji Harada Mitsuo Kameda Mitsue Suzuki Susumu Shigehara Susumu Mitsuhashi 《Journal of bacteriology》1967,93(4):1236-1241
The R21(TC) factor, obtained by transduction of the R10(TC.CM.SM.SA) factor with phage ε to group E Salmonella, is not transferable by the normal conjugal process. However, when R21(TC)+ transductants are infected with the F13 factor, the nontransferable R21(TC) factor acquires transmissibility by conjugation. R21(TC)+ conjugants of Escherichia coli K-12, to which only the R21(TC) factor was transmitted by cell-to-cell contact from an F′ R+ donor, were still unable to transfer their R21(TC) factor by conjugation. In crosses between Hfr and F−E. coli K-12 strains containing R21(TC), the gene responsible for tetracycline resistance was located on the E. coli K-12 chromosome between lac and pro, near lac. 相似文献
2.
Inversion of Transfer Modes and Sex Factor-Chromosome Interactions in Conjugation in Escherichia coli 总被引:8,自引:4,他引:4
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Brooks Low 《Journal of bacteriology》1967,93(1):98-106
A study was made of the mating properties of an unusual system of interconvertible donor strains of Escherichia coli K-12: Ra-1, Ra-2, and RaF+. The Ra-1 and Ra-2 strains are Hfr strains whose origins are widely separated on the chromosome and whose transfer modes proceed in the opposite direction from one another. When Ra-1 cells were mated with females, a small fraction of the donors transferred markers via the Ra-2 mode. This effect was enhanced by preconjugal ultraviolet (UV) treatment of the Ra-1 cells. Among the survivors of UV-treated Ra-1 cells, a few stable Ra-2 cells were found. When Ra-2 cells were used as the donors, some of them were found to mate via the Ra-1 mode, in analogy with the Ra-1 to Ra-2 alteration with inversion of F mentioned above. Related experiments suggested that the inversion occurs by detachment of the F factor from one Hfr origin locus, followed by reassociation of the F factor with the other Hfr origin locus. Both the Ra-1 and Ra-2 strains reverted spontaneously to an F+ strain, called RaF+. Cultures of RaF+ cells were found to mate primarily according to the Ra-1 and Ra-2 transfer modes, with smaller contributions also coming from transfer modes with origins elsewhere on the chromosome in a way which is similar to the transfer of markers from a normal F+ strain. The RaF+ sex factor was found to be wild type, whereas the chromosome was found to carry irregularities (sex factor affinity loci) at the locations of the Ra-1 and Ra-2 origins. Only about 10% of the donor capacity of the RaF+ strain was due to stable spontaneous Hfr cells in cultures of RaF+ cells. 相似文献
3.
The transposable drug-resistance element, Tn10, can serve as a region of homology to direct the insertion of an F'ts114 lac plasmid into the chromosome of Salmonella typhimurium. Derivatives of F'ts114 lac were constructed that carry Tn10 insertions; these plasmids were transferred to strains having a Tn10 insertion in the chromosome. Under these circumstances, Hfr formation requires homologous recombination between plasmid-borne and chromosomal Tn10 elements. The process is dependent on recA function and on the presence of both Tn10 elements. All Hfr's isolated from a given merodiploid show the same direction of transfer. Depending on the orientation of Tn10 in the F' plasmid, Hfr's transferring in either direction can be obtained from any chromosomal Tn10 insertion. Since Tn10 insertions can be generated in any region of the chromosome, this method permits the isolation of Hfr's with either direction of transfer having their origin at almost any predetermined site. The Hfr's constructed by this method are sufficiently stable for standard genetic mapping crosses, and they have also been used to generate new F' plasmids. Implicit in the results above is the possibility of determining the orientation of any chromosomal Tn10 insertion by constructing an Hfr using a standard F' Tn10 plasmid and determining the direction of chromosome transfer. The general approaches described here are applicable to other transposable elements and other bacterial systems. 相似文献
4.
Chlorate-resistant mutants ofS. typhimurium LT2 and LT7 and ofS. abony have been isolated, which are deficient in the biosynthesis of nicotinic acid and thiamin and in the fermentation of inositol. These mutants could be divided into 5 groups. The most likely gene order isnicB-chlG-thiB-inlB. This segment is transferred early in conjugation experiments with Hfr H2 and Hfr B2 as donors. In time-of-entry experiments with Hfr B2 as donor the segment entered about 3 minutes afterpur C. Consequently this segment maps in the 79- to 82-minutes region of the genetic map. From recombinant analysis of nic+ recombinants obtained in a four-point cross between Hfr B2 and ahis carBpur C del (nic chl G) acceptor the incorporation frequency of the transferred donor fragment was calculated to be about 0.41. The number of crossing-over events per minute length of the chromosome was about the same as in similar crosses betweenE. coli Hfr and F–. However, between thenic and thepur C markers it was much higher; it may therefore be inferred that there is a higher probability for a crossing-over event in the regions adjacent to the region that is deleted in the recipient.In crosses betweenS. abony Hfr H2 del (nic thi inl chl) and F– strains no recombinants were observed which have obtained the deletion from the donor. Nearly all auxotrophic or nic+ recombinants obtained in a cross between Hfr B2 and a F– del (nicBthiBchlGinlB) strain have inherited all markers of the donor, which are present in the deletion of the recipient. 相似文献
5.
Early transfer of genes determining transfer functions by some Hfr strains in Escherichia coli K12 总被引:10,自引:0,他引:10
Summary Sixty-eight Hfr strains were examined for their ability to transfer early in conjugation the transfer genes carried by the integrated sex factor. This was measured by mating these strains with F- phenocopied recipient cultures of strains carrying transfer-deficient Flac
+ factors, and then measuring the ability of the recipient strains to transfer lac
+ to a further recipient strain. Most Hfr strains did not complement the missing transfer functions, though in some strains complementation was observed. It is concluded that on the sex factors of different Hfr strains either the site at which integration occurs or the origin of transfer must vary. 相似文献
6.
DNA synthesis during bacterial conjugation: II. Is DNA replication in the Hfr obligatory for chromosome transfer ? 总被引:5,自引:0,他引:5
F-mediated transfer replication in a thymine-requiring Hfr of Eseherichia coli containing a thermosensitive dna B gene has been investigated. Ordinarily, DNA synthesis in the Hfr accompanies chromosome transfer at the non-permissive temperature. When the donors are, however, deprived of thymine, transfer occurs without any DNA synthesis. 相似文献
7.
Citrobacter freundii Hfr donor strains were isolated from a C. freundii strain harbouring a temperature-sensitive factor F ts 114 lac +, by selecting for integrative suppression of the ts 114 mutation. Three Hfr strains were characterized, which transfer their chromosomes in a linear and oriented order. The first strain transfers: O-aro +-ilv +-pur +-thr +-leu +-pro +, the second: O-ilv +-pur +-thr +-leu +-pro + and the third: O-ilv +-aro +-nad +-his +-pro +. The whole chromosome is transferred into the recipient cell within about 145 minutes. From these results we concluded that the linkage map of C. freundii is circular. Mating-pair formation on a membrane filter resulted in more recombinants being formed as compared with mating-pair formation in liquid medium. Furthermore the mating-pairs formed on a membrane were more stable. From one Hfr strain heterogenic F-prime factors could be isolated bearing the F ts 114 lac + genes from Escherichia coli and the pur + and/or ilv + genes from C. freundii. Preliminary mapping by interrupted mating indicated that the linkage map of C. freundii is in general very similar to those of E. coli, Salmonella typhimurium and Klebsiella aerogenes. 相似文献
8.
Specific Action of Sodium Dodecyl Sulfate on the Sex Factor of Escherichia coli K-12 Hfr Strains 总被引:13,自引:6,他引:7
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Noriko Inuzuka Sachiko Nakamura Manabu Inuzuka Munemitsu Tomoeda 《Journal of bacteriology》1969,100(2):827-835
A specific action of sodium dodecyl sulfate (SDS) on the sex (F) factor in the integrated state of Escherichia coli K-12 Hfr H strain is reported. Growth of Hfr cells in Penassay Broth containing SDS results in the elimination of part or all of the F factor, yielding low and nonfertile variants of defective Hfr type and F+ cells and also F− derivatives. Appearance of such variants was generally observed after the culture reached stationary phase. The frequencies of F− cells then increased. F− cells were usually isolated as the major population among survivors. Some defective variants of Hfr cells with an intermediate fertility between standard Hfr and F+ cells had lost sensitivity toward the male-specific ribonucleic acid phage M12. Other defective Hfr variants with as much or less fertility than standard F+ cells had also all lost sensitivity to phage M12. On single-colony isolation, they segregated nonfertile female H cells which, when infected with F, could restore high fertility with oriented transfer of the chromosome the same as that of the original Hfr H. Also, sensitivity to phage M12 was regained. Female H cells were characterized as those lacking fertility but still retaining a small segment of F or sfa locus at the original part of the chromosome, where newly infected F could attach. Similar results were obtained with two other Hfr strains. A possible mechanism of the specific action of SDS is discussed. 相似文献
9.
A non-transmissible tetracycline-resistance plasmid inE. coli was found to be transmissible by transduction and by conjugation with the aid of theE. coli K12 sex-factor.
Transfer of the tetracycline-resistance plasmid (R-tet) by transduction or conjugation to anE. coli K12 Hfr strain revealed that the plasmid was incompatible with the integrated F-factor. Selection for tetracycline-resistance
after conjugation or transduction yielded Hfr colonies which carried the tetracycline-resistance determinant as a chromosomal
marker. The tetracycline-resistance determinant was integrated at the 1 min region of theE. coli chromosome map (Taylor and Trotter, 1967) between the markersara andleu.
Apart from Hfr colonies with a chromosomal tetracycline-resistance determinant, F-gal+-mediated transfer of R-tet to strain Hfr R4 gave some colonies in which the tetracycline-resistance determinant was carried on a fused plasmid that,
besides the resistance determinant, contained thegal
+ marker of the original F-gal
+. This fused plasmid is transmissible and confers to an F− cell male-specific phage-sensitivity, like an F-factor does. It is suggested that this fused plasmid, which is compatible
with the integrated F-factor in the Hfr R4 cells, arose by recombination between F-gal
+ and R-tet. 相似文献
10.
DNA synthesis during bacterial conjugation. I. Effect of mating on DNA replication in Escherichia coli Hfr 总被引:1,自引:0,他引:1
Conjugation in Escherichia coli involves an oriented transfer of DNA from the Hfr to the F?. We have examined the course of DNA replication in a donor cell while it is transferring its DNA. Using isotopic density shift for estimating replication, we have shown that mating is accompanied by initiation of a new round of DNA replication in Hfr cells. With the onset of F-mediated transfer replication, the normal vegetative replication in the Hfr appears to be suppressed. Experiments with F′ donors indicate that the transfer of the chromosome is necessary for switching off vegetative replication. 相似文献
11.
P. G. de Haan A. H. Stouthamer Hendrica S. Felix Adriana K. Mol 《Antonie van Leeuwenhoek》1963,29(1):407-420
The transfer of theF episome fromEscherichia coli K 12 toE. coli B,Paracolobacter andKlebsiella was studied. The frequency of transfer of the episomal markers toE. coli B was very low. The large majority ofE. coli B cells which had received the episomal markerslac
+ orgal
+ were F–, which indicates that the episomal markers were stably integrated on the chromosome. Recombinants from K 12 F+ × B F– crosses were mostly F–. These results suggest that the multiplication of theF-factor ofE. coli K 12 is restricted inE. coli B. The transfer of theF-lac
+
Ad
+ episome fromE. coli K 12 toParacolobacter andKlebsiella strains was in most cases only possible when donor and acceptor strain were plated together on selective media. Stable incorporation of episomal markers was also found withParacolobacter coliforme. Paracolobacter aerogenoides andKlebsiella aerogenes strains could be infected withF-lac
+
Ad
+. The episomal markers were not incorporated and the episomes were easily lost, which indicates that these strains contained theF factor in the autonomous state. 相似文献
12.
Vegetative Replication and Transfer Replication of Deoxyribonucleic Acid in Temperature-Sensitive Mutants of Escherichia coli K-12 总被引:14,自引:10,他引:4
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Crosses were carried out at 34 C and 42 C between eight pairs of isogenic strains of Escherichia coli K-12. The donor and recipient of each pair carried the same mutation for temperature-sensitive deoxyribonucleic acid (DNA) synthesis; they differed only in the presence of F-lac in the donor and a spectinomycin-resistance marker in the recipient. A different temperature-sensitive mutation was present in each of the eight pairs, the eight temperature-sensitive mutations being located in at least two different genes. In all eight pairs, the transfer of F-lac occurred at high and equal rates at 34 C and 42 C, although vegetative DNA replication at 42 C was approximately 10−4 of that at 34 C. The transfer of F-lac at 42 C was accompanied in seven of the eight crosses by an equivalent amount of DNA synthesis in excess of that observed in the unmated controls. The DNA synthesized during transfer at 42 C was characterized by equilibrium centrifugation in cesium chloride and by its sedimentation velocity in sucrose gradients. It was found to have a density and a molecular weight characteristic of F-lac DNA. A small proportion of the material labeled during transfer was recovered in the form of covalently closed DNA. It is concluded that vegetative replication of the chromosome and transfer replication of F are separate processes, the former requiring at least two gene products which are nonessential for the latter. 相似文献
13.
The recB268::Tn10 mutation was introduced into the HfrH strain of Escherichia coli. Compared with recB F− and recB F+ cells, the viability of this mutant strain was much lower. Compared with wild-type HfrH, the recB derivative donated much shorter fragments of its chromosome to the recipient. It is suggested that the recB gene product (i.e., RecBCD enzyme) participates in Hfr transfer. 相似文献
14.
Genetic Transfer of the Vi Antigen from Salmonella typhosa to Escherichia coli 总被引:8,自引:5,他引:3
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The Vi antigen was expressed in a strain of Escherichia coli after transfer of the viaB locus from a Salmonella typhosa Hfr donor. 相似文献
15.
Site-specific integration of an F'' lac pro factor in the region of the replication origin (oriC) of E. coli 总被引:2,自引:0,他引:2
Summary An episome, F 128, which carries approximately 8x104 base pairs of chromosomal DNA homologous to the lac pro region of the E. coli chromosome, has been found to integrate into the oriC region of the chromosome in a site specific reaction. While the event appears to be recA-dependent, no homology between the episome and this region of the chromosome was detected. The Hfr strains formed result from the integration of intact F 128 molecules. The structure of the Hfr strains generated has been determined and their transfer properties analyzed. 相似文献
16.
Incompatibility of Integrated Sex Factors in Double Male Strains of ESCHERICHIA COLI 总被引:1,自引:0,他引:1
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Several strains of Escherichia coli K-12 harboring two F factors were isolated from Hfr x Hfr crosses. These strains were transiently capable of initiating chromosome transfer from two separate points of origin, and of transferring two different sex factors as integrated chromosomal markers. Each strain tested invariably reverted to a simple Hfr by loss of one of the inherited F factors. The F factor persisting in the revertant was, in nearly every case, that which had been inherited from the recipient Hfr parent. 相似文献
17.
Tn5-Induced Mutations in the Enterobacterial Phytopathogen Erwinia chrysanthemi 总被引:15,自引:5,他引:10
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Escherichia coli (2492/pJB4JI) matings with Erwinia chrysanthemi produced kanamycin resistant (Kmr) transconjugants, a majority of which were gentamicin sensitive (Gms). A small proportion (about 0.8%) of the Kmr Gms clones were either auxotrophic or failed to catabolize galacturonate (Gtu−). The R plasmid (pJB4JI) DNA was detected in the parent E. coli strain and in a Kmr Gmr transconjugant, but not in Kmr GmsE. chrysanthemi strains carrying Tn5-induced mutations. In Hfr crosses, Kmr (Tn5) was found linked with most mutations. A majority (>95%) of prototrophic recombinants were Kms, except for Leu+ and Arg+ recombinants which were 30 to 50% Kms. Spontaneous revertants were obtained for all markers except car, gtu, lys, thr, and trp. Prototrophic revertants, with the exception of Met+, Leu+, or His+ clones, were Kms. We conclude from both genetic and physical data that Tn5 transposed from pJB4JI into different sites on the chromosome of E. chrysanthemi. 相似文献
18.
Donor Strains of the Soft-Rot Bacterium Erwinia chrysanthemi and Conjugational Transfer of the Pectolytic Capacity 总被引:22,自引:16,他引:6
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Donor strains of Erwinia chrysanthemi ICPB EC16, a member of the soft-rot (pectolytic) section of the enterobacterial genus Erwinia, were obtained by chromosomal integration of an F′lac+ plasmid originating from Escherichia coli. These stable donor strains, selected from an unstable F′lac+ heterogenote by repeated platings of single Lac+ colonies on lactose minimal agar, do not segregate (as does the parent F′lac+ heterogenote) into Lac− or F− clones, in either the presence or absence of acridine orange. One representative donor strain (from the 12 that have been selected) has been examined in more detail; it can transfer ade+, gal+, gtu+ (utilization of galacturonate), his+, lac+, leu+, lys+, mcu+ (multiple carbohydrate utilization), pat+ (production of polygalacturonic acid trans-eliminase), thr+, and trp+ in a polarized manner to appropriate recipient strains of E. chrysanthemi; the frequencies of ade+, leu+, and thr+ transfer were higher than those of the other markers tested to date. This donor strain transfers lac+ genes during a 6-h mating on membranes; most of the Lac+ recombinants are donors of chromosomal markers. The kinetics of entry as well as the frequencies of transfer of chromosomal markers indicate that thr+ and leu+ enter the recipient as proximal markers and that lac+ enters as a distal marker. Analysis of the recombinants demonstrates close linkage between thr and leu, ade and thr, his and pat, and his and trp loci. The results suggest that the integration of F′lac+ into the chromosome of E. chrysanthemi has occurred at a region adjacent to the leu-thr loci, and that the chromosome is transferred in the following sequence: origin----leu--thr--ade--lys--mcu--pat--his--trp--gal--gtu--lac--F. Plant-tissue maceration occurs in Pat+ recombinants and not in Pat− recombinants, even though both form another pectolytic enzyme, hydrolytic polygalacturonase. This genetic evidence supports the idea that the E. chrysanthemi polygalacturonic acid trans-eliminase plays an essential role in bringing about plant-tissue maceration. 相似文献
19.
Isolation and characterisation of Hfr strains from a recombination-deficient strain of Escherichia coli 总被引:6,自引:0,他引:6
Summary A T6s RecA- strain carrying a lac proB deletion and Fts
lac
+ was challenged with phage T6 and survivors which were both T6r and Lac+ at 42° were tested for fertility. Among these were a number of Hfr strains which had their points of origin at or near the tsx locus and which still carried the recA allele. These arose in comparable frequencies in the RecA- strain and in a Rec+ analogue. We conclude that such integration does not require the RecA function. The rate of chromosome transfer was similar in one such RecA- Hfr and its Rec+ derivative; the yield of recombinants from the RecA- strain was slightly lower than from its Rec+ derivative. 相似文献
20.
Detection of transcribable recombination products following conjugation in rec+, reCB- and recC-strains of Escherichia coli K12 总被引:29,自引:0,他引:29
By crossing Hfr and F? strains of Escherichia coli which carry non-identical (but non-complementing) lacZ? mutations, the detection of β-galactosidase produced from LacZ+ recombination products is possible, beginning 60 minutes after transfer of the Hfr lac? allele. This system was used to show that when the F? cells carry recB?, almost normal amounts of LacZ+ enzyme are formed even though the number of viable recombinants is less than 1% of the Rec+ level. A similar result is found when the F? cells carry recC?. In contrast, LacZ+ enzyme activity is not detected either when RecA? F? cells are used or in a stable RecA? merodiploid carrying the two lacZ? alleles. 相似文献