The effect of strA mutation on symbiotic nitrogen fixation by Rhizobium meliloti.

Studies on 3H-dihydrostreptomycin accumulation and binding to ribosomes showed that ineffective strain CMts17 carries strB type mutation changing its membrane permeability to the drug. Introduction of high level streptomycin resistance of strA type into strain CMts17 was correlated with acquisition of effectiveness and membrane permeability to the drug. This suggests that changes in membrane permeability, responsible for ineffectiveness of strain CMts17, can be reversed by strA mutation.     

The effect of some herbicides on lactate dehydrogenase activity

The effect of herbicides N-(phosphonomethyl)glycine (glyphosate), 2-methyl-4-chlorophenoxyacetic acid (MCPA), 2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine (atrazine) and the respective commercial preparations “Round-up”, “Aminex”, and “Zeazin”, on the activity of lactate dehydrogenase isolated from germinating soybean seeds and beef heart were compared. The main aim of the work was to compare the effect of the herbicides on lactate dehydrogenase isolated from animal and plant material. Simultaneously the effect of herbicides and the respective commercial preparations was compared. Substantial differences resulting from the two comparisons indicate differences in the structure of active centres of lactate dehydrogenase isolated from animal and plant material, and also a considerable effect of additives and surfactants in commercial preparations.     

The effect of herbicides on the root system of wheat plants

Three commercial herbicides, at the rates normally recommended for selective weed control in cereal crops, deformed the roots of spring wheat plants grown in sand culture. Affected roots produced large numbers of short swollen lateral branches which later grew on to form tassel-like bundles of side roots. Only the mecoprop and MCPA components of the mixtures were responsible for the deformities; ioxynil and dicamba had no effect. Mecoprop applied to the rooting medium severely deformed the roots but foliar application also resulted in some abnormalities. The effects of the herbicides on root and shoot dry weights were not correlated with their deformative effects.     

The analysis of the effect of different herbicides on Johnson-grass in maize

The Johnson-grass (Sorghum halepense /L./Pers) is a perennial monocotyledon, its gen centre is in the Near East. Botanically this weed belongs to the monocotyledone class, Poaceae (Gromineae) family and within this, together with the maize to the Andropogonoideaei subfamily. At the time of the First National Weed Survey (1947-1953) it has not been found in Hungary. In the periods of the 2nd (1969-1971), 3rd (1987-1988) and 4th Survey it occupied the 94th, 18th and 10th place of importance, respectively. Because of its rapid multiplication and spreading after the second weed survey (1969-1971) and because of its very complicated and difficult control, the Plant Protection Division of our Ministry of Agriculture ranged it in 1974 into the category of "Dangerous weed". Johnson grass is the 6th most important weed in the world. A small plot field trial was carried out in Szabadszentkirtly (12th May 2006) in maize. The effect of pre- and post emergent herbicides was investigated against Johnson grass germinating from seed and sprouting from rhizome. The treatments were done in 4 repeats with Stomp 330 EC, Dual Gold 960 EC, Merlin WG, Wing EC, Guardian Max and Monsoon herbicides. The number of weeds germinating after spraying and the number of damaged weeds were counted and compared to the control plots 10 days after the treatments and then weekly. Parallel to the field trial, a "soil culture in pots" trial in glasshouse was also carried out in which the effect of the above mentioned herbicides was investigated against Johnson grass germinating from seed. During on experiments all the herbicides showed an excellent herbicide activity until the 2nd week of treatment, although the precipitation of the second week was only 8.4 mm. Under the given experimental conditions the best herbicide effect until the 4th week, in both experimental phases were given by Merlin WG.     

The effect of microorganisms on phytotoxicity of herbicides. III. Interaction of Bacillus sp. 72 with Venzar.

Interactions between Venzar and some metabolites of Bacillus ps. 72 were studied. This strain was found to produce flavonoids increasing the phytotoxicity of Venzar. Venzar action was also increased by NH4+ ions released aboundantly by the bacterial strain.     

Influence of TRPV3 mutation on hair growth cycle in mice

We recently reported that Gly573Ser substitution of the transient receptor potential cation channel, subfamily V member 3 (TRPV3) caused hair loss in DS-Nh mice. To further elucidate the effects of this mutation on the development of the spontaneous hairless phenotype, we examined the temperature-response to epidermal sheets from DS-Nh and DS mice. It was indicated that the mutation was gain-of-function. We also performed genetic and histological analyses with both strain skins. DNA microarray data revealed that the levels of keratin-associated protein 16-1, 16-3, and 16-9 genes related to the anagen phase were decreased in the skins of DS-Nh mice compared with those of three days old DS mice. Histological analysis revealed that the anagen phase persisted in DS-Nh mice, and that the telogen phase was seen in DS but not DS-Nh mice at 21 days of age. Regulation of TRPV3 appears to be important for appropriate hair development in rodents.