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Cooperation of two distinct cis-acting elements is necessary for the S phase-specific activation of the wheat histone H3 promoter 总被引:2,自引:2,他引:0
Norihiro Ohtsubo Takuya Nakayama Hidetaka Kaya Rie Terada Ko Shimamoto Tetsuo Meshi Masaki Iwabuchi 《The Plant journal : for cell and molecular biology》1997,11(6):1219-1225
We have previously shown that the proximal promoter region (−185 to +57) of the wheat histone H3 gene ( TH012 ) is sufficient for regulating S phase-specific expression of a reporter GUS gene. To define the cis -acting element(s) responsible for S phase-specific expression, GUS fusion genes under the control of wild-type or variously mutated H3 promoters were stably introduced into cultured rice Oc cells and their temporal expression was analyzed during the cell cycle by quantitative S1 analysis. The S phase-specific expression of the full-sized promoter (−1716 to +52) was significantly impaired by short internal deletions disrupting the type I element from −175 to −158 (CCACGTCACCaATCCGCG), composed of the Hex (CCACG-TCA) and reverse-oriented Oct (GATCCGCG) motifs. Moreover, the H3 proximal promoters (−184 to +52) harboring base-substitution mutations in either or both of the Hex and Oct motifs could no longer activate gene expression during the S phase. These results indicate that the type I element is the first cis-acting element identified responsible for the S phase-specific expression of plant histone genes. Results also suggested the presence of a redundant cis -acting element(s) responsible for S phase-specific expression in the H3 far-upstream region (−1716 to −185). 相似文献
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Adjacent DNA sequences modulate Sox9 transcriptional activation at paired Sox sites in three chondrocyte-specific enhancer elements
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Bridgewater LC Walker MD Miller GC Ellison TA Holsinger LD Potter JL Jackson TL Chen RK Winkel VL Zhang Z McKinney S de Crombrugghe B 《Nucleic acids research》2003,31(5):1541-1553
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Structural determinants for transcriptional activation by cAMP-responsive DNA elements 总被引:28,自引:0,他引:28
P J Deutsch J P Hoeffler J L Jameson J C Lin J F Habener 《The Journal of biological chemistry》1988,263(34):18466-18472
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The concentrations of trace elements in several mollusk, arthropod, echinoderm, and tunicata species were determined by photon
activation analysis. The samples were freeze-dried, pulverized by a mill, and fractionated through a 200-mesh sieve. Three
standard materials were used for comparative standards: NIST SRM-1566a Oyster Tissue, NIST SRM-1577b Bovine Liver, and NRCC
TORT-1 Lobster. The samples and comparator standards were sealed in silica tubes and irradiated for 3 h by 30-MeV bremsstrahlung
from a linear accelerator at Tohoku University. By measuring gamma-ray spectra, the concentrations of 16 elements (As, Br,
Ca, Cr, Cu, Fe, I, Mg, Mn, Mo, Na, Ni, Pb, Rb, Sr, and Zn) were determined. 相似文献
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Role of alpha-fetoprotein regulatory elements in transcriptional activation in transient heterokaryons.
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The requirements for activation of the mouse alpha-fetoprotein (AFP) gene in transient heterokaryons were investigated. For this purpose, the 7-kilobases of DNA flanking the 5' end of the AFP gene were linked to a mouse major histocompatibility complex (MHC) class I structural gene. The fusion gene was stably integrated at different sites into mouse L-cells, which do not transcribe the AFP gene. Transient heterokaryon fusions demonstrated that the silent AFP-MHC gene and the endogenous AFP gene were activated by factors present in HepG2 cells, a liver-derived cell line, but not by those in HeLa cells. Activation was detected at the protein level in single heterokaryons by using monoclonal antibodies against the cell surface protein and at the mRNA level in populations of cells. The AFP promoter alone was sufficient for activation could be used for DNA transfer strategies to identify genes which can activate AFP promoter elements in trans. 相似文献
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