共查询到20条相似文献,搜索用时 31 毫秒
1.
2.
3.
4.
5.
H Lechevalier 《Microbiological reviews》1976,40(1):241-258
6.
BISHOP PM 《Proceedings of the Royal Society of Medicine》1955,48(12):1032-1038
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
A brief sketch is presented of Vavilov's career, his main contributions to science and personal qualities. The imporatnce of his work in genetics, agro-ecology, plant breeding, the experimental taxonomy of crop plants and genetic resources conservation is also emphasized. 相似文献
18.
19.
I Tait 《BMJ (Clinical research ed.)》1981,283(6292):647-648
20.
S Kochhar V S Lamzin A Razeto M Delley H Hottinger J E Germond 《European journal of biochemistry》2000,267(6):1633-1639
The role of three histidine residues (His205, His296 and His303) and Asp259, important for the catalysis of NAD+-specific D-lactate dehydrogenase, was investigated using site-directed mutagenesis. None of these residues is presumed to be involved in coenzyme binding because Km for NADH remained essentially unchanged for all the mutant enzymes. Replacement of His205 with lysine resulted in a 125-fold reduction in kcat and a slight lowering of the Km value for pyruvate. D259N mutant showed a 56-fold reduction in kcat and a fivefold lowering of Km. The enzymatic activity profile shifted towards acidic pH by approximately 2 units. The H303K mutation produced no significant change in kcat values, although Km for pyruvate increased fourfold. Substitution of His296 with lysine produced no significant change in kcat values or in Km for substrate. The results obtained suggest that His205 and Asp259 play an important role in catalysis, whereas His303 does not. This corroborates structural information available for some members of the D-specific dehydrogenases family. The catalytic His296, proposed from structural studies to be the active site acid/base catalyst, is not invariant. Its function can be accomplished by lysine and this has significant implications for the enzymatic mechanism. 相似文献