Population dynamics of a natural red deer population over 200 years detected via substantial changes of genetic variation

Most large mammals have constantly been exposed to anthropogenic influence over decades or even centuries. Because of their long generation times and lack of sampling material, inferences of past population genetic dynamics, including anthropogenic impacts, have only relied on the analysis of the structure of extant populations. Here, we investigate for the first time the change in the genetic constitution of a natural red deer population over two centuries, using up to 200‐year‐old antlers (30 generations) stored in trophy collections. To the best of our knowledge, this is the oldest DNA source ever used for microsatellite population genetic analyses. We demonstrate that government policy and hunting laws may have strong impacts on populations that can lead to unexpectedly rapid changes in the genetic constitution of a large mammal population. A high ancestral individual polymorphism seen in an outbreeding population (1813–1861) was strongly reduced in descendants (1923–1940) during the mid‐19th and early 20th century by genetic bottlenecks. Today (2011), individual polymorphism and variance among individuals is increasing in a constant‐sized (managed) population. Differentiation was high among periods (F_ST > ***); consequently, assignment tests assigned individuals to their own period with >85% probability. In contrast to the high variance observed at nuclear microsatellite loci, mtDNA (D‐loop) was monomorphic through time, suggesting that male immigration dominates the genetic evolution in this population.     

Anthropological Professionalization and the Virginia Indians at the Turn of the Century

In the late 19th and early 20th centuries the Indians of Virginia, like marginalized native communities throughout the Southeast, were enmeshed in struggles over their identity, as "one-drop" rules were increasingly applied and formalized. At that time several scholars, including James Mooney, wrote on the Powhatan tribes in American Anthropologist, the nascent journal of a professionalizing discipline. Previously, most works on the Virginia Indians had been published locally; after a brief florescence on the national scene (roughly corresponding to the 300th anniversary of English settlement in Powhatan territory), that pattern resumed. The works published on Virginia Indians in this period, and the contrast with their relative invisibility in professional journals over the following decades, cast light on U.S. anthropology's development as a profession. This article examines the transition from local to national organizations from the standpoint of ethnographic inclusion of such marginalized peoples. [Keywords: Powhatan Indians, ethnic identity, professionalization, localism, American Anthropologist]     

Traditional food and herbal uses of wild plants in the ancient South-Slavic diaspora of Mundimitar/Montemitro (Southern Italy)

ABSTRACT: BACKGROUND: In Europe, only a limited number of cross-cultural comparative field studies or meta-analyses have been focused on the dynamics through which folk plant knowledge changes over space and time, while a few studies have contributed to the understanding of how plant uses change among newcomers. Nevertheless, ethnic minority groups and/or linguistic "isles" in Southern and Eastern Europe may provide wonderful arenas for understanding the various factors that influence changes in plant uses. METHODS: A field ethnobotanical study was carried out in Mundimitar (Montemitro in Italian), a village of approx. 450 inhabitants, located in the Molise region of South-Eastern Italy. Mundimitar is a South-Slavic community, composed of the descendants of people who migrated to the area during the first half of the 14th century, probably from the lower Neretva valley (Dalmatia and Herzegovina regions). Eighteen key informants (average age: 63.7) were selected using the snowball sampling technique and participated in in-depth interviews regarding their Traditional Knowledge (TK) of the local flora. RESULTS: Although TK on wild plants is eroded in Montemitro among the youngest generations, fiftyseven taxa (including two cultivated species, which were included due to their unusual uses) were quoted by the study participants. Half of the taxa have correspondence in the Croatian and Herzegovinian folk botanical nomenclature, and the other half with South-Italian folk plant names. A remarkable link to the wild vegetable uses recorded in Dalmatia is evident. A comparison of the collected data with the previous ethnobotanical data of the Molise region and of the entire Italian Peninsula pointed out a few uses that have not been recorded in Italy thus far: the culinary use of boiled black bryony (Tamus communis) shoots in sauces and also on pasta; the use of squirting cucumber (Ecballium elaterium) juice for treating malaria in humans; the aerial parts of the elderberry tree (Sambucus nigra) for treating erysipelas in pigs; the aerial parts of pellitory (Parietaria judaica) in decoctions for treating haemorrhoids. CONCLUSIONS: The fact that half of the most salient species documented in our case study - widely available both in Molise and in Dalmatia and Herzegovina - retain a Slavic name could indicate that they may have also been used in Dalmatia and Herzegovina before the migration took place. However, given the occurrence of several South-Italian plant names and uses, also a remarkable acculturation process affected the Slavic community of Montemitro during these last centuries. Future directions of research should try to simultaneously compare current ethnobotanical knowledge of both migrated communities and their counterparts in the areas of origin.     

Characterization of CYP1A2, CYP2C19, CYP3A4 and CYP3A5 polymorphisms in South Brazilians

Potential causes of variability in drug response include intrinsic factors such as ethnicity and genetic differences in the expression of enzymes that metabolize drugs, such as those from Cytochrome P450 (CYPs) superfamily. Pharmacogenetic studies search for genetic differences between populations since relevant alleles occur with varying frequencies among different ethnic populations. The Brazilian population is one of the most heterogeneous in the world, resulting from multiethnic admixture of Amerindians, Europeans, and Africans across centuries. Since the knowledge of CYP allele frequency distributions is relevant to pharmacogenetic strategies and these data are scarce in the Brazilian population, this study aimed to describe genotype and allele distributions of 15 single nucleotide polymorphisms (SNPs) at CYP 1A2, 2C19, 3A4, and 3A5 genes in African and European descents from South Brazil. A sample of 179 healthy individuals of European and African ancestry was genotyped by the MassARRAY SNP genotyping system. CYP3A5*3, CYP1A2*1F, CYP3A4*1B, and CYP2C19*2 were the most frequent alleles found in our sample. Significant differences in genotype and allelic distribution between African and European descents were observed for CYP3A4 and CYP3A5 genes. CYP3A4*1B was observed in higher frequency in African descents (0.379) than in European descents (0.098), and European descents showed higher frequency of CYP3A5*3 (0.810) than African descents (0.523). Our results indicate that only a few polymorphisms would have impact in pharmacogenetic testing in South Brazilians. Further studies with larger sample sizes are required also among other Brazilian regions.     

MSH2 c.1452-1455delAATG is a founder mutation and an important cause of hereditary nonpolyposis colorectal cancer in the southern Chinese population

Hereditary nonpolyposis colorectal cancer (HNPCC) accounts for approximately 2% of all colorectal cancer (CRC) cases and is the most common hereditary CRC syndrome. We have previously reported a high incidence of microsatellite instability (MSI) and germline mismatch repair (MMR) gene mutations in young Hong Kong Chinese with CRC. Ongoing studies at the Hereditary Gastrointestinal Cancer Registry in Hong Kong have revealed a unique germline MSH2 c.1452-1455delAATG mutation that has not been reported in other ethnic groups. Detailed analysis showed that this specific MSH2 mutation constituted 21% of all germline MMR gene mutations and 36% of all MSH2 germline mutations identified. We designed a specific PCR-based diagnostic test on paraffin-embedded tissues and identified this germline mutation in 2 (1.5%) of 138 consecutive patients with early-onset CRC (<46 years of age at diagnosis). Haplotype analysis was performed using 11 microsatellite markers located between D2S391 and D2S123. All 10 families had the same disease haplotype, suggesting a founder effect. These 10 families all originated from the Chinese province of Guangdong, which historically included Hong Kong. It is the most populous of the Chinese provinces, with a population of >93 million. Further analysis suggested that this founder mutation may date back to between 22 and 103 generations ago. The identification of this MSH2 founder mutation has important implications for the design of mutation-detection strategies for the southern Chinese population. Since there were major emigrations from Hong Kong and Guangdong province during the 19th and 20th centuries, this finding is also significant for Chinese communities worldwide.     

The Genetic Legacy of the Expansion of Turkic-Speaking Nomads across Eurasia

The Turkic peoples represent a diverse collection of ethnic groups defined by the Turkic languages. These groups have dispersed across a vast area, including Siberia, Northwest China, Central Asia, East Europe, the Caucasus, Anatolia, the Middle East, and Afghanistan. The origin and early dispersal history of the Turkic peoples is disputed, with candidates for their ancient homeland ranging from the Transcaspian steppe to Manchuria in Northeast Asia. Previous genetic studies have not identified a clear-cut unifying genetic signal for the Turkic peoples, which lends support for language replacement rather than demic diffusion as the model for the Turkic language’s expansion. We addressed the genetic origin of 373 individuals from 22 Turkic-speaking populations, representing their current geographic range, by analyzing genome-wide high-density genotype data. In agreement with the elite dominance model of language expansion most of the Turkic peoples studied genetically resemble their geographic neighbors. However, western Turkic peoples sampled across West Eurasia shared an excess of long chromosomal tracts that are identical by descent (IBD) with populations from present-day South Siberia and Mongolia (SSM), an area where historians center a series of early Turkic and non-Turkic steppe polities. While SSM matching IBD tracts (> 1cM) are also observed in non-Turkic populations, Turkic peoples demonstrate a higher percentage of such tracts (p-values ≤ 0.01) compared to their non-Turkic neighbors. Finally, we used the ALDER method and inferred admixture dates (~9th–17th centuries) that overlap with the Turkic migrations of the 5th–16th centuries. Thus, our results indicate historical admixture among Turkic peoples, and the recent shared ancestry with modern populations in SSM supports one of the hypothesized homelands for their nomadic Turkic and related Mongolic ancestors.     

From wetland to commercial centre: the natural history of Wyspa Spichrzów (“Granary Island”) in medieval Gdańsk,northern Poland

This paper describes the analyses of pollen, non-pollen palynomorphs, macroremains and geochemistry in sediments from archaeological excavations at Wyspa Spichrzów (“Granary Island”) in Gdańsk, northern Poland. The aim of this study was to reconstruct the environmental conditions in this part of the town in the period preceding its occupation and during its transformation with the establishment of a trade centre and an increasing number of granaries, warehouses and workshops. The results show that this area was originally covered by wetlands typical of river oxbows, with a landscape formed by alder woods, shallow pools of water, fens and patches of wet meadows. Around the 9th–10th centuries, a distinct lowering of the groundwater table reduced the pools, and the alder stands also reduced. These changes coincided with the Medieval Climatic Anomaly. Drying of the ground could have been an important factor enabling expansion of settlement in the Gdańsk area. Between the 10th and 13th–14th centuries, the area around the investigated sites was probably used as pasture, as shown by high frequencies of coprophilous fungal spores. A large representation of cereal pollen and pollen and macroremains of field weeds reflects crop transport and storage on the island from ca. the 13th/14th centuries in the northern part of the area and the 15th/16th centuries in its southern part. The increasing human impact caused development of a rich flora associated with human activities, habitat enrichment by nitrogen and phosphorus, and heavy metal pollution from the beginning of the 13th century.     

Desert ants do not acquire and use a three-dimensional global vector

Background

Desert ants (Cataglyphis fortis) are central place foragers that navigate by means of path integration. This mechanism remains accurate even on three-dimensional itineraries. In this study, we tested three hypotheses concerning the underlying principles of Cataglyphis' orientation in 3-D: (1) Do the ants employ a strictly two-dimensional representation of their itineraries, (2) do they link additional information about ascents and descents to their 2-D home vector, or (3) do they use true 3-D vector navigation?

Results

We trained ants to walk routes within channels that included ascents and descents. In choice tests, ants walked on ramps more frequently and at greater lengths if their preceding journey also included vertical components. However, the sequence of ascents and descents, as well as their distance from nest and feeder, were not retraced. Importantly, the animals did not compensate for an enforced vertical deviation from the home vector.

Conclusion

We conclude that Cataglyphis fortis essentially represents its environment in a simplified, two-dimensional fashion, with information about vertical path segments being learnt, but independently from their congruence with the actual three-dimensional configuration of the environment. Our findings render the existence of a path integration mechanism that is functional in all three dimensions highly unlikely.     

Inequality of Experience of Dental Caries between Different Ethnic Groups of Brazilians Aged 15 to 19 Years

Introduction

The aim of this study was to assess inequality of experience of dental caries, based on race/ethnicity, among Brazilian adolescents aged 15 to 19 years in 2010 and test whether socioeconomic indicators fully explain ethnic differences in dental caries.

Methods

Data from a National Oral Health Survey conducted in Brazil in 2010 was analysed. Race/ethnicity was self-assigned and modified to White, African descents, East Asian descents, Mixed Race and Indigenous descents. The prevalence of caries experience by race/ethnic group in 2010(n = 5,367) was calculated. Further analysis included conceptual hierarchical modelling and mediation analysis.

Results

Caries experience was 76.9% in 15 to 19 year old Brazilians in 2010. While African descents were 32% more likely to have caries experience than Whites, Mixed Race were 69% more likely to have caries experience than Whites. Hierarchical conceptual modelling analysis confirmed the highly significant association between caries and race/ethnicity. Mixed Race and East Asian descents were 1.44 (95% CI 1.24–1.67) and 1.81 (95% CI 1.02–3.20) times more likely to experience caries than Whites after adjusting for age, sex, education and income. The difference in the likelihood of experiencing caries between Whites and African descents was not statistically significant after adjusting for years of education and family income. The results of mediation analysis confirmed that inequality of caries experience between Whites and Mixed Race and East Asian descents was mediated through education and income. The likelihood that Mixed Race and East Asian descents would experience caries compared to Whites was attenuated, by 14.8% and by 9.5% respectively, after adjusting for years of education and income.

Conclusions

Data analysis demonstrated that Whites have benefited more from the significant reduction in dental caries experience in 15 to 19 year old Brazilians, as compared to African descents and Mixed Race. Education and income fully explained ethnic inequalities in experience of dental caries between Whites and African descents, and largely explained inequalities between Whites and Mixed Race.     

Effect of maternal obesity on diabetes development in adult rat offspring

This study aimed to evaluate whether maternal obesity leads to the onset of diabetes in adult Wistar rats offspring. MSG solution neonatally administration induced obesity in rats (F(1)MSG group, n=30); and saline solution was also administrated to control rats (F(1)CON group, n=13). In 3rd month of age, both control and MSG groups were mated for offspring (generation F(2)), named as F(2)CON, n=28 and F(2)MSG groups, n=15; and so both generations were studied until 7th month of life. Lee Index was measured for experimental obesity validation from 5th to 7th month. Glycemia was weekly determined during pregnancy and monthly from 3rd to 7th month. In the end of experimental period all rats were submitted to oral glucose tolerance test (OGTT), with estimation of total area under the curve (AUC); and insulin tolerance test (ITT). Rats were then anesthetized and killed. Data were statistically analyzed with significance level of p<0.05. Lee Index has confirmed obesity in all MSG rats. Glycemic levels comparisons between generations showed significant maternal interference in control and MSG groups. OGTT analysis showed higher glycemia in obese rats (F(1)MSG) and their offspring (F(2)MSG) as compared to their respective controls; and MSG groups increased AUC from OGTT. As regards ITT, F(2)MSG showed higher glycemia at 30 and 120 min, suggesting a delay of insulin action decreasing. Although glucose intolerance and insulin resistance clinical conditions represent as a factors for type 2 Diabetes mellitus development, this experimental model proposal was not efficient to induce type 2 Diabetes mellitus, but for obesity developing, glucose intolerance and insulin resistance in successive generations of rats.     

The medical profession and congenital malformations (1900-1979)

Physicians' interest in congenital malformations has varied greatly during the last two centuries. After an acme in the 19th century, teratology was of little inteerest to the medical profession during the first four decades of the 20th century. Since then a variety of events have again made birth defects important. An attempt is made to explain the waxing and waning of the physicians' attention to this age-old problem.     

Impact of dietary sterols on life-history traits of a caterpillar

Although lepidopteran larvae have been shown to metabolise a number of different phytosterols, their life-history traits in response to these different phytosterols may vary with the species. Using the specialist moth, Plutella xylostella (Linaeus) (Lepidoptera), growth, development and fecundity on artificial diets containing host and non-host phytosterols were measured over two generations. Because of the nature of the experimental design, maternal effects linked to dietary sterol could be assessed. In both generations, survival was highest on diets containing sitosterol as the primary phytosterol. A significant decrease in survival on stigmasterol, a non-host phytosterol, was observed between generations. As expected, survival on a control diet with only trace-sterols decreased between generations. Larval duration and pupal duration in both generations was longest on the diets containing the non-host phytosterols, stigmasterol and spinasterol, and on the trace-sterol diet. Finally, fecundity was affected by dietary sterol. In the first generation, fewer viable eggs were produced on the stigmasterol and trace-sterol diets than on wheatgerm control diet. In both generations, dietary sterol significantly influenced percentage egg viability. Overall, these results indicate that differences in phytosterol structure can have pronounced effects on life-history traits, especially when they are projected to the population level. The implications of these results are discussed with respect to phytosterol use by phytophagous insects and their host plant affiliations.     

Cross-Cultural Ethnobiology in the Western Balkans: Medical Ethnobotany and Ethnozoology Among Albanians and Serbs in the Pešter Plateau,Sandžak,South-Western Serbia

An ethnobiological study concerning the medical ethnobotany and ethnozoology of two neighbouring communities of Serbians and Albanians living in the Pešter plateau (south-western Serbia) was conducted, the latter representing a diasporic community that immigrated to the area approximately three centuries ago. Sixty-two botanical taxa used in 129 plant-based remedies and 204 folk plant uses were recorded. In addition, 31 animal-derived remedies and 27 mineral or non-indigenous products were also documented. Approximately half of the recorded phytotherepeutical uses have been recorded for the first time in the ethnobotany of the Western Balkans and more than one-third of these uses have no correlation with Western evidence-based phytotherapy. Moreover, while both communities use approximately the same number of medicinal plants, two-thirds of the botanical taxa, but only one-third of plant folk medical uses are found in common among the two communities. These findings demonstrate that the two communities, although having lived in close proximity to each other during the past three centuries and in a relatively low biodiverse environment, have maintained or developed unique phytotherapeutical trajectories. The differences between the two folk medical biologies of these communities are reflective of the specific history of the Albanian diaspora, and of the complex processes of its cultural adaptation over the last three centuries.     

History of human settlement, cultural change and interference with the marine environment

Humans have been present in the Wadden Sea area since the end of the last ice age, but their perception of and interference with their marine environment has changed over time. In this paper, I will give an overview on the interactions between man and nature since the 6th millennium B.C., on the opportunities for human settlement as well as on restrictions posed by the maritime environment. Only after many centuries of passive adaptation did the local farming population begin to modify their immediate surroundings. They made a living as cattle breeders, supplemented this with fishing, hunting, weaving, salt production and peat digging. Efforts to transform the agricultural landscape did not start before the 11th century A.D., when the first dikes and canals were constructed. The consequences were profound. By the end of the Middle Ages, the dikes had become totally indispensable. The land under cultivation was perceived as a sacred inner world, conflicting sharply with the marine environment outside its flood-gates. This essentially dichotomous world-view held out until the 19th and early 20th centuries. As we will see, however, the actual settlement history had been marked by various gains and losses, by successes as well as by setbacks. Not only did humans destroy valuable natural resources, but they also created alternative habitats for novel species. I conclude that the initial tendency towards increasing natural and cultural diversity has been reversed during the last few centuries. Yet, mounting conservationist concerns may cause a turning-point.

Occupation and land-use history of a medium mountain from the Mid-Holocene: A multidisciplinary study performed in the South Cantal (French Massif Central)

A multidisciplinary investigation combining archaeological and palaeoecological approaches (pollen, micro-charcoal, major elements geochemistry, and radiocarbon data) has been carried out since 2000 in the southern Cantal (French Massif Central) in order to achieve a better understanding of the environmental/anthropogenic interactions in a mountain ecosystem ranging from 1000 to 1600 m a.s.l. from the Mid-Holocene to the end of Modern Times. This medium mountain area must be considered as a complex landscape shaped during a long-term land-use history. Pollen and archaeological evidences suggest a human frequentation as early as the beginning of the 6th millennium BC. For the following periods, different stages related to the human settlement and anthropogenic activities of land clearance and agro-pastoralism have been documented: the final Neolithic/Early Bronze Age and the Roman period (mainly the 3rd and 4th centuries AD). Middle Ages (between the 10th and the 12th centuries AD) and Modern Times (since the 14th–16th centuries AD) appear to be two key phases revealing complex spatial patterns of land-use.     

Is the flavin-deficient red blood cell common in Maremma, Italy, an important defense against malaria in this area?

There is a high prevalence of a familial flavin-deficient red blood cell in Ferrara province in the Po delta in northern Italy, believed to have been selected for by malaria which was endemic from the 12th century. In the present study, activities of FAD-dependent red-cell glutathione reductase (EGR) in the Grosseto area of Maremma on the west coast of Italy where malaria was endemic from 300 B.C. are compared both with activities in the Ferrara area and with activities where there was no history of endemic malaria--in the Florence area and in London in people of Anglo-Saxon origin. EGR activities were similar in Grosseto and Ferrara and were significantly lower than in Florence and London. As previously found in Ferrara, low EGR activity in Grosseto was shown to be unrelated to low dietary riboflavin intake. These findings in Grosseto, suggesting selection by malaria, are particularly interesting because, unlike the situation in Ferrara and most other malarial areas, the prevalence of thalassemia and glucose-6-phosphate dehydrogenase deficiency is very low, and they do not appear to have been selected for in Maremma. It is possible that a flavin-deficient red cell, known to inhibit growth of the malaria parasite, was an important protecting factor in the population of this area over the centuries.     

Age estimates of two common mutations causing factor XI deficiency: recent genetic drift is not necessary for elevated disease incidence among Ashkenazi Jews

The type II and type III mutations at the FXI locus, which cause coagulation factor XI deficiency, have high frequencies in Jewish populations. The type III mutation is largely restricted to Ashkenazi Jews, but the type II mutation is observed at high frequency in both Ashkenazi and Iraqi Jews, suggesting the possibility that the mutation appeared before the separation of these communities. Here we report estimates of the ages of the type II and type III mutations, based on the observed distribution of allelic variants at a flanking microsatellite marker (D4S171). The results are consistent with a recent origin for the type III mutation but suggest that the type II mutation appeared >120 generations ago. This finding demonstrates that the high frequency of the type II mutation among Jews is independent of the demographic upheavals among Ashkenazi Jews in the 16th and 17th centuries.     

Monitoring in the western part of the Dutch Wadden: Sea—sea level and morphology

This paper deals with the methods and results of monitoring sea level (tidal gauges) and with the analysis of depth sounding data. Possible future monitoring by menas of remote sensing techniques will be presented. Some trends, based on water level and morphological monitoring, have been established in the western part of the Dutch Wadden sea: (a) the relative mean sea level is rising persistently by 15 cm/century, observed since the middle of the 19th century. The tidal range is increasing as well; (b) the cross-sectional areas of the most western Dutch Wadden tidal inlets have been increasing over the last two centuries; (c) the intertidal zones of the Texelstroom and Vliestroom tidal basins have shown an increase of surface area over the last decades. The Borndiep tidal basin shows a decrease of 4% of the area shallower than 5 m — Dutch Ordinance Level (DOL) over the last two centuries. Several factors prevent serious predictions about future morphological developments of the tidal Wadden flats: (a) man induced interfering factors, e.g. the construction of the harbour revetments of Den Helder, the construction of the Enclosure Dike and construction of sand drift dikes stabilising the Wadden islands; (b) the limited scope of the present analysis that deals only with the western part of the Dutch Wadden Sea. Presented at the VI International Wadden Sea Symposium (Biologische Anstalt Helgoland, Wattenmeerstation Sylt, D-2282 List, FRG, 1–4 November 1988)     

Phylogeny of Callibaetis (Ephemeroptera: Baetidae) based on morphology of nymphs and adults

Callibaetis is considered to be one of the most problematic genera among mayflies by a series of taxonomic inaccuracies that have accumulated over the last two centuries. Despite these taxonomic problems, two independent hypotheses of species groups have been proposed. In the first hypothesis, three species groups for North America were proposed, and in the second, three species groups were proposed for South American. In these hypotheses, the generic delimitation and monophyly of Callibaetis have not been evaluated under a cladistic framework. Taking this into account, the objectives of this study were to verify the monophyly of Callibaetis and whether the groups of species proposed for the genus are corroborated as natural. The matrix included 128 morphological characters and one habitat character, 119 discrete characters were compiled (101 of nymphs and 18 of adults), and six imaginal characters are related to pigmentation pattern. Continuous characters were ratios and were represented by 10 characters (nine for nymphs and one for adults). The data set was analysed under implied weights. Group support was estimated with relative Bremer support and frequency differences. The results corroborated the monophyletic nature of Callibaetis and the generic status of Callibaetoides; however, the groups proposed for North and South American Callibaetis species were not corroborated. Our study indicated four groups of species for the genus, which we proposed as subgenera: Callibaetis, Abaetetuba subgen. n. Aiso subgen. n. and Cunhaporanga subgen. n.