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1.
Isolation of Halotolerant Thermus spp. from Submarine Hot Springs in Iceland 总被引:1,自引:0,他引:1
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Jakob K. Kristjansson Gudmundur O. Hreggvidsson Gudni A. Alfredsson 《Applied microbiology》1986,52(6):1313-1316
Thermophilic, aerobic bacteria of the genus Thermus were isolated from submarine alkaline hot springs in Iceland. Five submarine hot springs were sampled, and all had viable counts of Thermus spp. of about 103 CFU/ml. All submarine strains grew in the presence of NaCl at 3% or higher, but no strains from terrestrial hot springs would grow at concentrations higher than 1% NaCl. The growth rate of submarine Thermus strains was not stimulated by NaCl and was reduced at NaCl concentrations higher than 1%. The pattern of growth of these isolates on single carbon sources was similar to that of terrestrial isolates. 相似文献
2.
A. Ismail-Ben Ali M. El Bour L. Ktari H. Bolhuis M. Ahmed A. Boudabbous L. J. Stal 《Journal of applied phycology》2012,24(3):525-534
Marine macroalgae surfaces constitute suitable substrata for bacterial colonization which are known to produce bioactive compounds. Thus, hereby we focused on heterotrophic aerobic bacteria species associated with coralline red alga Jania rubens (northern coast of Tunisia, southern Mediterranean Sea) and their inhibition against several microbial marine and terrestrial species. The whole collection (19 isolates, J1 to J19) was identified, based on their 16S ribosomal RNA gene sequences as Proteobacteria (14 strains), Bacteroidetes (4 strains) and Firmicutes (1 strain). Thirty-six percent of the isolates (J2, J9, J11, J13, J16, J17 and J18) were antibiotic-like producers with in vitro inhibition against Gram + and Gram ? bacteria and the yeast Candida albicans. Highest level of inhibition was revealed for the isolates J2, J9 and J13 identified respectively as Bacillus, Aquimarina and Pseudomonas, with strong activity against Staphylococcus aureus, Micrococcus and C. albicans, with inhibition diameters of 25 to 35 mm shown by drop test assay on T soy agar plates. Furthermore, we tested inhibition of J. rubens crude organic extracts against human and marine bacteria as well as against all J. rubens isolates, in order to determine the degree of affinity of the epibionts to their proper host. The recovery of strains with antimicrobial activity suggests that J. rubens represent an ecological niche which harbors a specific microbial diversity worthy of further secondary metabolites investigation. 相似文献
3.
Jessica A. Sameoto Anna Metaxas 《Journal of experimental marine biology and ecology》2008,367(2):131-141
In laboratory experiments, we examined the effect of haloclines and determined whether the presence of food patches overrides this effect on larval vertical distribution of the sea star Asterias rubens, the sea urchin Strongylocentrotus droebachiensis and the mussel Mytilus edulis. We experimentally constructed haloclines in which the salinity of the bottom water layer was 35 and that of the top layer was 21, 24, 27, and 30 (21/35, 24/35, 27/35, and 30/35) for A. rubens and S. droebachiensis, and 24, 27, 30 and 32 (24/35, 27/35, 30/35, and 32/35) for M. edulis. For each species and stage, additional halocline treatments (A. rubens: 24/32 and 27/32; 4-arm S. droebachiensis: 21/29 and 24/32; 6-arm S. droebachiensis: 24/29 and 24/32; M. edulis: 27/32 and 30/32) were used to determine whether the larval response to inhibitory salinity gradients was due to the absolute salinity of the top layer or the relative salinity difference between the two layers. Also, we measured the density of A. rubens and M. edulis to determine whether the specific gravity of larvae can explain the observed vertical distributions. Larvae aggregated at and below the halocline and these aggregations were more pronounced with increasing strength of the vertical salinity gradient. Threshold salinities in the top layer which inhibited ~ 100% of the larvae from crossing the halocline were 24 for A. rubens and M. edulis, and 21 for S. droebachiensis. These distributional patterns were not the result of larval density, which was greater than all treatment water densities for M. edulis and S. droebachiensis and lower for A. rubens. The effect of the presence of a food patch at inhibitory haloclines (A. rubens: 24/35 and 27/35; 4-arm S. droebachiensis: 21/34 and 24/34; M. edulis: 27/35) was determined by using three algal densities: 0, 5000 or 10 000 cells ml- 1Thalassiosira pseudonana in either the top or the bottom water layer. For both A. rubens and M. edulis, the number of larvae at the halocline increased in the presence of a food patch, but this effect did not depend on algal density in the patch. For 4-arm S. droebachiensis, there was no effect of the presence of a food patch on larval vertical distribution. Our results suggest that low salinity may act as a barrier to vertical movement and that the presence of food patches above the halocline may strengthen the larval aggregation response to inhibitory haloclines. 相似文献
4.
Incidence of Plasmids in Thermus spp. Isolated in Yellowstone National Park 总被引:4,自引:4,他引:0
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Forty-eight strains of Thermus spp. were isolated from thermal sites in Yellowstone National Park, Wyo., and 62.5% showed evidence of plasmid DNA. Attempts to assign function to the plasmid DNA were unsuccessful, and the presence of plasmid DNA could not be correlated with antibiotic or heavy metal resistance. A number of these cryptic plasmids are now being investigated for their potential as vectors for molecular cloning in Thermus spp. 相似文献
5.
Distribution of Genes for Synthesis of Trehalose and Mannosylglycerate in Thermus spp. and Direct Correlation of These Genes with Halotolerance
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Susana Alarico Nuno Empadinhas Catarina Sim?es Zélia Silva Anke Henne Ana Mingote Helena Santos Milton S. da Costa 《Applied microbiology》2005,71(5):2460-2466
In this study we correlate the presence of genes leading to the synthesis of trehalose and mannosylglycerate (MG) in 17 strains of the genus Thermus with the ability of the strains to grow and accumulate these compatible solutes in a defined medium containing NaCl. The two sets of genes, namely, otsA/otsB for the synthesis of trehalose and mpgS/mpgP for the synthesis of MG, were necessary for the growth of Thermus thermophilus in a defined medium containing up to 6% NaCl. Strains lacking a complete otsA gene did not grow in defined medium containing >2% NaCl. One strain of T. thermophilus lacking the genes for the synthesis of MG did not grow in a medium with >1% NaCl. We did not identify any of these genes in the type strains of the other seven species of Thermus, and none of those strains grew in defined medium with 1% NaCl. The results strongly indicate that the combined accumulation of trehalose and MG is required for optimal osmotic adjustment. 相似文献
6.
Distribution of Thermus spp. in Icelandic Hot Springs and a Thermal Gradient 总被引:7,自引:5,他引:2
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The growth range in nature of bacteria belonging to the genus Thermus was investigated by sampling 55 different hot springs in Iceland. The springs ranged in temperature from 32 to 99°C, and in pH from 2.1 to 10.1. Viable counts of Thermus spp. ranging from 10 to 104 CFU/100 ml of spring water were found in 27 of the springs sampled. The temperature range for these bacteria was found to be 55 to 85°C, and the pH range was from about 6.5 to above 10. Thermus spp. were found in springs containing up to 1 mM dissolved sulfide and having conductivity up to 2,000 μS/cm. The distribution of Thermus spp. in a hot spring thermal gradient was also investigated and found to agree well with the overall distribution in individual springs. 相似文献
7.
《Gene》1996,172(1):117-119
A gene encoding L-lactate dehydrogenase (LDH; EC 1.1.1.27) from Deinococcus radiodurans (Dr) was cloned and sequenced. The deduced amino acid (aa) sequence was compared to those of the Thermus aquaticus (Ta) and T. caldophilus (Tc) LDH. The similarity of the G+C contents between the Dr and Thermus genes permits the comparison of the aa sequences of LDH without considering the difference in the G+C contents. Compared to the mesophilic Dr LDH, increased numbers of hydrophobic aa, together with hydrophilic Arg, were found in the LDH from Ta and Tc, suggesting that these features would contribute to protein thermostability in part via hydrophobic and electrostatic interactions in the protein globule. Deinococcus would be a suitable mesophilic counterpart for Thermus to investigate the thermostability of proteins. 相似文献
8.
The lysozyme of bacteriophage φIN93 was purified to apparent homogeneity with Carboxymethyl Sepharose and Hydroxyapatie columns from lysates of the phage grown on Thermus aquaticus TZ2. The enzyme is a single polypeptide chain with a molecular weight of 33,000. From the determined N-terminal amio acids of the enzyme, the locus of the gene was specified on a φIN93 genome. The enzyme was not similar to egg white lysozyme, T4 phage lysozyme, or lambda phage lysozyme. The enzyme, φIN93 lysozyme, was found to be a novel type of thermophilic lysozyme, which lyses specifically Thermus sp. cells, and exhibited conspicuous thermal stability at 95 °C for 1 h in the presence of β-mercaptoethanol. 相似文献
9.
ESR and succinate oxidase activity were used to investigate the membrane phase-transitions of an extreme thermophile, Thermus T351, over an 80°C temperature range in whole cells, membrane particles, and extracted lipid suspension. Three phase transitions were observed using both techniques. These occurred at about 19°C, 39°C and 66°C. The transition at 19°C is unusual in that the Arrhenius plot for succinate oxidase is concave upwards, implying an increase in activation energy (Ea) with increased temperature. 相似文献
10.
New thermostable enzyme activities of seven Thermus strains were compared using the API ZYM system. All the strains exhibited high levels of - and -glycosidases, esterase (C4) and esterase-lipase (C8) activities intracellularly. Only T. thermophilus HB8 (ATCC 27634) showed -glucosidase and esterase activities in the supernatant. According to the intensity of -galactosidase activity, Thermus strains were divided in three groups. Group 0, which showed a weak -galactosidase activity, included Thermus spp. ATCC 31674 (T351) and 27978 (X-1) as well as T. thermophilus ATCC 27634 (HB8). Group I which consisted of T. aquaticus ATCC 25104 (YT-1), ATCC 25105 (Y-VII-51B) and Thermus sp. ATCC 27737 (T2), had a specific activity of approximately 40.0 U mg–1 and galactose as inducer. T. aquaticus ATCC 31558 (group 2) was particularly effective for -galactosidase production (2840 U) with a specific activity of 98 U mg–1. For each strain, galactose (0.5%) was a better inducer of -galactosidase production than lactose (1%). The detection of -galactosidase activity was dependent on the derivative chromogenic substrates used (naphthyl or nitrophenol coupled to sugar). Oligosaccharides were synthesized from cellobiose, lactulose, maltose or lactose as substrates at high temperature in some strains of Thermus. 相似文献
11.
《Journal of biochemical and biophysical methods》1996,31(1-2):31-37
Dye-ligand chromatography has been used successfully for the purification of extracellular thermostable proteinases from thermophilic Bacillus and Thermus cultures. Single step purification factors of up to 115-fold (for Thermus protease) and 2195-fold (for Bacillus protease) were obtained. Elution studies suggested that the mode of binding involved the enzyme active sites. The method was readily scaleable to 600 l volume. 相似文献
12.
Vatsala Dwivedi Kirti Kumari Sanjay Kumar Gupta Rekha Kumari Charu Tripathi Pushp Lata Neha Niharika Amit Kumar Singh Roshan Kumar Aeshna Nigam Nidhi Garg Rup Lal 《Indian journal of microbiology》2015,55(4):357-365
A Gram negative, yellow pigmented, rod shaped bacterium designated as RLT was isolated from a hot water spring (90–98 °C) located at Manikaran in Northern India. The isolate grows at 60–80 °C (optimum, 70 °C) and at pH 7.0–9.0 (optimum pH 7.2). Phylogenetic analysis of 16S rRNA gene sequences and levels of DNA–DNA relatedness together indicate that the new isolate represents a novel species of the genus Thermus with closest affinity to Thermus thermophilus HB8T (99.5 %) followed by Thermus arciformis (96.4 %). A comparative analysis of partial sequences of housekeeping genes (HKG) further revealed that strain RLT is a novel species belonging to the genus Thermus. The melting G+C content of strain RLT was calculated as 68.7 mol%. The DNA–DNA relatedness value of strain RLT with its nearest neighbours (>97 %) was found to be less than 70 % indicating that strain RLT represents a novel species of the genus Thermus. MK-8 was the predominant respiratory quinone. The presence of characteristic phospholipid and glycolipid further confirmed that strain RLT belongs to the genus Thermus. The predominant fatty acids of strain RLT were iso-C17:0 (23.67 %) and iso-C15:0 (24.50 %). The results obtained after DNA–DNA hybridization, biochemical and physiological tests clearly distinguished strain RLT from its closely related species. Thus, strain RLT represents a novel species of the genus Thermus for which the name Thermus parvatiensis is proposed (=DSM 21745T= MTCC 8932T).
Electronic supplementary material
The online version of this article (doi:10.1007/s12088-015-0538-4) contains supplementary material, which is available to authorized users. 相似文献13.
Two members of the genus Thermus were examined for their resistance to toxic inorganic compounds. They both proved to be fairly resistant to tellurite and selenite and to many other heavy metal salts. Cell extracts of Thermus thermophilus HB8 and of T. flavus AT-62 catalyze the reduction of K2TeO3 in a reaction which is dependent on NADH oxidation. 相似文献
14.
T.L. Norall A.C. Mathieson J.A. Kilar 《Journal of experimental marine biology and ecology》1981,54(2):119-136
The reproduction and stature of the red algae Callophyllis cristata (C. Ag.) Kuetz., Membranoptera alata (Huds.) Stackh., Phycodrys rubens (Huds.) Batt., and Ptilota serrata Kuetz. were recorded from subtidal populations at Appledore Island, Maine, U.S.A., with respect to time and depth. Only Membranoptera alata exhibited a conspicuous seasonal fluctuation of reproduction. A vertical gradient of reproduction was evident, with reduced levels of reproduction in shallow populations of Phycodrys rubens and Ptilota serrata, as well as deep populations of Phycodrys rubens, Membranoptera alata and Callophyllis cristata. Differential stratification of the reproductive phases of Ptilota serrata occurred with higher frequencies of tetrasporic plants in deep populations and of cystocarpic plants in shallow populations. In contrast, the haploid and diploid plants of the other three species showed similar distributional patterns. Membranoptera alata, Phycodrys rubens, and Callophyllis cristata showed a conspicuous decrease in stature during maximum reproduction. 相似文献
15.
Identification of iron-reducing<Emphasis Type="Italic"> Thermus</Emphasis> strains as<Emphasis Type="Italic"> Thermus scotoductus</Emphasis> 总被引:1,自引:0,他引:1
Balkwill DL Kieft TL Tsukuda T Kostandarithes HM Onstott TC Macnaughton S Bownas J Fredrickson JK 《Extremophiles : life under extreme conditions》2004,8(1):37-44
Thermus strain SA-01, previously isolated from a deep (3.2 km) South African gold mine, is closely related to Thermus strains NMX2 A.1 and VI-7 (previously isolated from thermal springs in New Mexico, USA, and Portugal, respectively). Thermus strains SA-01 and NMX2 A.1 have also been shown previously to grow using nitrate, Fe(III), Mn(IV) or SO as terminal electron acceptors and to be capable of reducing Cr(VI), U(VI), Co(III), and the quinone-containing compound anthraquinone-2,6-disulfonate. The objectives of this study were to determine the phylogenetic positions of the three known metal-reducing Thermus strains and to determine the phylogenetic significance of metal reduction within the genus Thermus. Phylogenetic analyses of 16S rDNA sequences, BOX PCR genomic fingerprinting, and DNA–DNA reassociation analyses indicated that these strains belong to the previously described genospecies T. scotoductus. The morphologies and lipid fatty acid profiles of these metal-reducing strains are consistent with their identification as T. scotoductus; however, the T. scotoductus strains tested in this study evinced a wide intraspecies variability in some other phenotypic traits, e.g., carbon substrate utilization and pigmentation. Iron reduction occurred in all strains of T. scotoductus tested except the mixotrophic, sulfur-oxidizing strain IT-7254. Thermus strains belonging to other species did not reduce Fe(III) to Fe(II) or reduced it only poorly.Communicated by J. Wiegel 相似文献
16.
Effect of the epizoic rotifer Brachionus rubens on the population growth of three cladoceran species
Using population densities and growth rates as criteria, we studied interactions between the epizoic rotifer Brachionus rubens and each of three cladoceran species differing in size and reproductive rates — Daphnia carinata, Moina macrocopa and Ceriodaphnia rigaudi. In all mixed — species experiments, B. rubens existed in both the epizoic mode, attached to the cladoceran host, and in the free-swimming mode. Rotifer population growth rates were significantly depressed in the presence of M. macrocopa, presumably as a consequence of exploitative and interference competition. The largest cladoceran, D. carinata probably did not suppress B. rubens, because the epizoic component of the rotifer population escaped from the deleterious effects of mechanical interference. Peak population numbers and initial population growth rates reached by all three cladocerans were lower in the presence of B. rubens, probably because of the adverse effects of the epizoic infestation, which was maximal on D. carinata and least on C. rigaudi. In mixed-species cultures of D. carinata and M. macrocopa, the presence of B. rubens helped D. carinata coexist with M. macrocopa, which otherwise would have suppressed the Daphnia. 相似文献
17.
Magdalena Plotka Anna-Karina Kaczorowska Aleksandra Stefanska Agnieszka Morzywolek Olafur H. Fridjonsson Stanislaw Dunin-Horkawicz Lukasz Kozlowski Gudmundur O. Hreggvidsson Jakob K. Kristjansson Slawomir Dabrowski Janusz M. Bujnicki Tadeusz Kaczorowski 《Applied and environmental microbiology》2014,80(3):886-895
In this study, we present the discovery and characterization of a highly thermostable endolysin from bacteriophage Ph2119 infecting Thermus strain MAT2119 isolated from geothermal areas in Iceland. Nucleotide sequence analysis of the 16S rRNA gene affiliated the strain with the species Thermus scotoductus. Bioinformatics analysis has allowed identification in the genome of phage 2119 of an open reading frame (468 bp in length) coding for a 155-amino-acid basic protein with an Mr of 17,555. Ph2119 endolysin does not resemble any known thermophilic phage lytic enzymes. Instead, it has conserved amino acid residues (His30, Tyr58, His132, and Cys140) that form a Zn2+ binding site characteristic of T3 and T7 lysozymes, as well as eukaryotic peptidoglycan recognition proteins, which directly bind to, but also may destroy, bacterial peptidoglycan. The purified enzyme shows high lytic activity toward thermophiles, i.e., T. scotoductus (100%), Thermus thermophilus (100%), and Thermus flavus (99%), and also, to a lesser extent, toward mesophilic Gram-negative bacteria, i.e., Escherichia coli (34%), Serratia marcescens (28%), Pseudomonas fluorescens (13%), and Salmonella enterica serovar Panama (10%). The enzyme has shown no activity against a number of Gram-positive bacteria analyzed, with the exception of Deinococcus radiodurans (25%) and Bacillus cereus (15%). Ph2119 endolysin was found to be highly thermostable: it retains approximately 87% of its lytic activity after 6 h of incubation at 95°C. The optimum temperature range for the enzyme activity is 50°C to 78°C. The enzyme exhibits lytic activity in the pH range of 6 to 10 (maximum at pH 7.5 to 8.0) and is also active in the presence of up to 500 mM NaCl. 相似文献
18.
Two relatively simple procedures are described for the purification of phosphofructokinase from the extreme thermophile, Thermus X-1. The native enzyme has a molecular weight of 1.32 × 105 and contains four apparently identical polypeptide chains. One substrate, fructose-6-phosphate, induces a cooperative protein transition while the other substrate, ATP, does not. Phosphoenolpyruvate functions as an avid negative effector and ADP is a positive effector. The enzyme has an optimum temperature for catalysis of 80 °C. Persistence of the catalytic and allosteric properties over the temperature range 20–80 °C suggests that the same protein structure is retained throughout this temperature range. Thermus X-1 phosphofructokinase is more stable to inactivation by heat, urea, guanidine hydrochloride or acidification than the phosphofructokinases obtained from the mesophilic organisms Escherichia coli and Clostridium pasteurianum. Comparison of the amino acid compositions of the three enzymes indicates no substantive differences in their hydrophobicity, hydrogen bonding potential or average residue size. The markedly elevated optimum temperature for catalysis exhibited by the Thermus enzyme appears to result from stabilization of its catalytically functional conformational to a reversible thermal inactivation above 40 °C and to ligation of the substrate fructose-6-phosphate. 相似文献
19.
《Systematic and applied microbiology》2022,45(6):126361
Biological denitrification is a significant process in nitrogen biogeochemical cycle of terrestrial geothermal environments, and Thermus species have been shown to be crucial heterotrophic denitrifier in hydrothermal system. Five Gram-stain negative, aerobic and rod-shaped thermophilic bacterial strains were isolated from hot spring sediments in Tibet, China. Phylogenetic analysis based on 16S rRNA gene and whole genome sequences indicated that these isolates should be assigned to the genus Thermus and were most closely related to Thermus caldifontis YIM 73026T, and Thermus brockianus YS38T. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the five strains and the type strains of the genus Thermus were lower than the threshold values (95% and 70%, respectively) recommended for bacterial species, which clearly distinguished the five isolates from other species of the genus Thermus and indicated that they represent independent species. Colonies are circular, convex, non-transparent. Cell growth occurred at 37–80 °C (optimum, 60–65 °C), pH 6.0–8.0 (optimum, pH 7.0) and with 0–2.0% (w/v) NaCl (optimum, 0–0.5%). Denitrification genes (narG, nirK, nirS, and norB genes) detected in their genomes indicated their potential function in nitrogen metabolism. The obtained results combined with those of morphological, physiological, and chemotaxonomic characteristics, including the menaquinones, polar lipids, and cellular fatty acids showed that the isolates are proposed as representing five novel species of the genus Thermus, which are proposed as Thermus hydrothermalis sp. nov. SYSU G00291T, Thermus neutrinimicus sp. nov. SYSU G00388T, Thermus thalpophilus sp. nov. SYSU G00506T, Thermus albus sp. nov. SYSU G00608T, Thermus altitudinis sp. nov. SYSU G00630T. 相似文献
20.
The byssus production of the blue mussel Mytilus edulis L. was studied in the laboratory in the presence of the metabolites of the following animals: a predator (a starfish Asterias rubens L.) and several species competing with the mussel in White Sea fouling communities (a bivalve Hiatella arctica L. and a solitary ascidian Styela rustica L.). The byssus threads and attachment plaques produced by each mussel per day were counted. The number of byssus threads and plaques was smallest in pure sea water and in the presence of metabolites produced by conspecific individuals. 相似文献