共查询到20条相似文献,搜索用时 15 毫秒
1.
The ultrastructure of the glandular trichomes and secretory ducts of Grindelia pulchella was studied. Plastids, mitochondria and endoplasmic reticulum are involved in the secretory process of both, trichomes and ducts. A special tissue with “transfer cells” is associated with the duct epithelial cells. The secretion is produced in the transfer cells and then is transferred to the duct epithelial cells where it accumulates in the vacuoles. The occurrence of cavities within the cell walls of the trichome cells and duct epithelial cells is described. The secretion is accumulated between the cell wall and the cuticle of these cells. When the cuticle is broken the secretion is released. We conclude that granulocrine secretion operates in this species. 相似文献
2.
慈菇匍匐茎中分泌道的初步研究 总被引:2,自引:0,他引:2
慈茹匍蔔茎的分泌道是裂生的胞间道,分布于匍匐茎的基本组织中。单个分泌道原始细胞起始于离茎端约1毫米处的基本分生组织中,原始细胞经分裂形成5—7个上皮细胞包围着中央的裂生腔隙,成为管道系统。上皮细胞无鞘细胞包围。上皮细胞中高尔基体和内质网发达,并溢出小囊泡向着分泌道腔隙面壁的质膜附近迁移,乳汁中亦存在大量完整的小囊泡。上皮细胞和外围薄壁细胞之间的壁层具有大量胞间连丝,小囊泡和内质网的膜结构与胞间连丝末端相接,同时可见上皮细胞的质膜在数处反折内陷,形成袋状结构,在与上皮细胞相对的薄壁细胞内也有同样现象出现,袋状结构内含小形颗粒或囊泡,并在结构上显示出上皮细胞与相邻薄壁细胞间存在着活跃的物质交流。由此认为。代谢物质以整体小囊泡的形式经胞间连丝或内陷的质膜向分泌道迁移是物质运输和分泌的可能方式之一。在电镜下观察,液泡中的积聚物与乳汁十分相似,液泡可能是乳汁的贮存场所之一。 相似文献
3.
A volatile sex pheromone is produced in an adult female-specific gland located on the anterior of the last abdominal tergite of the female German cockroach, Blattella germanica (L.). In this area, the cuticle forms deep depressions in which a large number of cuticular orifices are located. The cuticular orifices are connected to secretory cells via cuticular ducts surrounded by duct cells. The pheromone gland exhibits a clear developmental maturation in relation to sexual maturation of the female. The secretory cells of a newly formed gland in the imaginal female are small and contain few secretory vesicles. The amount of extractable pheromone in the gland is low on day-0 but it increases with age and peaks on day-6. The secretory cells in a mature day-6 gland are characterized by a large number of electron-lucid secretory vesicles. abundant RER and SER, a large nucleus and a long, convoluted end apparatus which is lined with numerous microvilli. The contents of the secretory vesicles are exocytosed into extracellular reservoirs at the base of microvilli and then transported to the cuticular surface through the long ducts. The supportive function of the duct cell in the glandular organization and developmental regulation of the gland are discussed. 相似文献
4.
Dr. Heiner Birkenbeil 《Cell and tissue research》1983,229(2):433-441
Summary The formation and secretion of ecdysteroid by the prothoracic gland cells of Galleria mellonella (Insecta, Lepidoptera) were investigated electron microscopically and immunocytochemically. The moulting hormone ecdysone becomes first evident in vesicles and tubules of the smooth endoplasmic reticulum (SER). The SER forms secretory granules in which ecdysone was shown immunocytochemically. The Golgi apparatus seems not to be directly involved in ecdysone secretion. The secretory granules are released from the cells by exocytosis.Supported by the Sächsische Akademie der Wissenschaften zu LeipzigThe author is grateful to Mrs. Angelika Schmidt for her excellent assistance 相似文献
5.
Fine structure of the excretory system of the deep posterior (Ebner's) salivary glands of the human tongue 总被引:1,自引:0,他引:1
Human deep posterior lingual glands (von Ebner's glands) are located beneath the circumvallate papillae. They are formed by tubuloalveolar adenomeres, intercalated ducts and excretory ducts coming together in the main excretory duct. The tubuloalveolar cells, pyramid-shaped, show large and dense secretory granules (clear cored) throughout the cytoplasm, rare basal folds and packed cisternae of rough endoplasmic reticulum (RER) at the basal pole. The columnar cells of the intercalated ducts are arranged in a monolayer. They are characterized by dense, clear-core secretory granules (mostly in the apical cytoplasm), a basal nucleus, well-developed RER and Golgi apparatus, and thin filaments distributed in supra- and perinuclear cytoplasm. Striated ducts are absent. Excretory ducts, coming together in the main duct, are lined by a bistratified epithelium. The inner layer consists of columnar cells showing bundles of tonofilaments with scarce secretory activity. The outer layer is composed of basal cells lying on the basal lamina. The main excretory duct, which opens at the bottom of the vallum, shows a stratified epithelium. The outer side is composed of 2-3 layers of malpighian cells lying on the basal lamina. The inner side consists of a single layer of cuboidal-columnar cells with dense apical granules and well-developed organelles synthesizing and condensing secretions. These cells interpolate with goblet cells, rare mitochondria-rich cells, ciliated cells and numerous small globous cells showing a clear matrix and lacking secretory granules. The cilia show a 9 + 2 microtubular structure with basal bodies provided with striated rootlets. Myoepithelial cells surround with their processes the basal portions of the secretory cells and the intercalated ducts. The conclusions concern some comparative aspects and some hypothesis on the functional role of goblet cells, ciliated cells and epithelial cells lining the different ducts, also in relation to the final secretory product. 相似文献
6.
Charles H. Dorsey 《Experimental parasitology》1975,37(1):37-59
Cercariae of Schistosoma mansoni in daughter sporocysts in Biomphalaria glabrata were studied with the electron microscope to observe the maturing process of their acetabular glands. The undifferentiated acetabular gland displays its enlarged basal area (fundus) and extended narrow process (duct) before other organ systems are recognized. Its fundus contains a prominent nucleus and subcellular organelles typical of active secretory cells.The secretory granules of the postacetabular glands are formed in a milieu of dilated rough endoplasmic reticulum and Golgi. Two morphologically different secretory granules are produced: (1) homogeneously granular ones, and (2) other granular ones with electron dense bodies in their matrices. Mostly, the homogeneously granular ones are produced first in the fundus and are forced into the ducts as the other type is formed.The secretory granules of preacetabular glands are formed from translucent vacuoles which arise from an environment of endoplasmic reticulum and Golgi. Two morphologically different secretory granules are produced: (1) one type has a homogeneous dense matrix, and (2) the other type has a less dense matrix containing electron-lucid bodies.The duct of an undifferentiated acetabular gland has either filamentous material or microtubules dispersed in its cytoplasm. Once microtubules are formed, they persist during the life of the cercaria. The microtubules are believed to have possibly two functions: (1) to support the long duct, and (2) to assist the movement of the secretory granules into the channels of the ducts where they remain until released during host penetration.Few of the subcellular organelles associated with secretory granules formation are seen in the duct except the area in close proximity to the fundus; thus, the few secretory granules produced in the duct are in this region. 相似文献
7.
8.
【目的】蟋螽是直翅目中唯一具有吐丝筑巢行为的类群。本研究旨在探讨蟋螽丝腺的结构特点。【方法】应用解剖学观察、免疫荧光、苏木精-伊红染色、PAS苏木精染色、扫描电镜和透射电镜等方法从细胞水平对黑缘烟蟋螽Capnogryllacris nigromarginata丝腺的显微与超微结构进行了观察。【结果】黑缘烟蟋螽丝腺由导管和腺泡构成。腺泡由鞘细胞延伸形成的结缔组织鞘包围。腺泡的主体有4种细胞,分别为Ⅰ型分泌细胞、Ⅱ型分泌细胞、围细胞和腔细胞。Ⅰ型和Ⅱ型分泌细胞为大的腺细胞,形状不规则。分泌细胞细胞核很大,胞质内有大量的内质网和分泌颗粒。Ⅰ型分泌细胞靠近腺泡中心,PAS-苏木精染色表明Ⅰ型分泌细胞内含糖蛋白,Ⅱ型分泌细胞在腺泡外周,位于Ⅰ型分泌细胞与围细胞或结缔组织鞘之间。腔细胞分散在分泌细胞之间,包围形成胞外运输分泌物的通道。围细胞与鞘细胞接触,具有由细胞膜内陷形成的微绒毛腔,胞质内有大量的线粒体。围细胞微绒毛腔与腔细胞包围的细胞外运输通道相连,分泌细胞分泌的颗粒聚集在分泌细胞和胞外运输通道之间的连接处,并将分泌物排出至胞外运输通道。多个腺泡的胞外运输通道汇集到由单层细胞组成的丝腺导管。单层导管细胞靠近管腔外围具有规则排列的质膜内陷和大量伸长的线粒体;靠近管腔的一侧具连续的细胞膜突起,在导管壁的表皮下紧密排列。【结论】黑缘烟蟋螽丝腺分泌细胞分为Ⅰ型分泌细胞和Ⅱ型分泌细胞。分泌物质产生及分泌过程依次经过分泌细胞、腔细胞包围的胞外通道、分支导管、总导管和唾窦。其中在腺泡细胞之间,分泌物向外运输过程中,围细胞微绒毛腔的微丝束可能对分泌物的外排提供推动力。 相似文献
9.
The bilateral salivary glands, ducts, and nerves of the giant garden slug Limax maximus control the secretion of saliva and its transport to the buccal mass. Each salivary nerve, which originates at the buccal ganglion, contains over 3000 axon profiles. The axons innervate the musculature of the duct and branch within the gland. The salivary duct is composed of several muscular layers surrounding an epithelial layer which lines the duct lumen. The morphology of the duct epithelium indicates that it may function in ion or water balance. The salivary gland contains four major types of secretory cells. The secretory products are released from vacuoles in the gland cells, and are presumably transported by cilia in the collecting ducts of the gland into the larger muscular ducts. 相似文献
10.
Effects of Wounding and Fungus Inoculation on Terpene Producing Systems of Maritime Pine 总被引:3,自引:0,他引:3
Cheniclet, C. 1987. Effects of wounding and fungus inoculationon terpene producing systems of maritime pine.J. exp.Bot. 38: 15571572. The qualitative and quantitative changes occurring in terpenecompounds (resin acids and mono- and sesquiterpene hydrocarbons)in phloem and xylem tissues after wounding and after inoculationwith a beetle-associated fungus have been compared on maritimepines from the same clone. Simultaneously, structural eventsoccurring in phloem have been observed, especially those concerningresin ducts and cell systems associated with terpene secretion. The terpene accumulation resulting from wounding is weak andremains limited to a short period and to a restricted tissuearea. In contrast, mycelium inoculation results in an exponentialenrichment (x 60 after 28 d in proximal phloem) as the enrichedzone enlarges, indicating a diffusive process. In wounded phloem the neosynthesis of terpenes takes place inreactivated or supernumerary resin ducts which display structuralfeatures similar to those of active secretory cells in normalconditions. In fungus-moculated phloem, secretory ducts are first reactivated,but soon undergo a lysis, and are no longer functional whenthe rate of terpene accumulation is increasing quickly. Theformation of terpene compounds is delocalized and occurs innon-specific intra- or extracellular spaces formed by the lysisof cell contents and/or cell walls. The synthesis probably resultsfrom the random meeting of enzymes, which can be synthesizedin living parenchyma cells or released by lysis of structuresinvolved in physiological terpene secretion (leucoplasts andendoplasmic reticulum), and of the corresponding substrates,which may be provided by degradation of cell contents (polyphenolics,hpids) or by the sieve flow. Key words: Terpene secretion, wounding, fungus inoculation 相似文献
11.
Routine light microscopy and transmission and scanning electron microscopy were used to describe and compare the livers of larval lampreys, Lampetra lamottenii before and during infection of the bile ducts by the nematode, Truttaedacnitis stelmioides. The hepatocytes of uninfected animals differ from other lamprey species in that they contain abundant glycogen, smooth endoplasmic reticulum (SER), and lipoprotein indicating that the liver may be involved in glucose metabolism. Infestation of the biliary tree by T. stelmioides coincides with alterations to the hepatocytes. These changes include dilation of the bile canaliculi, smooth endoplasmic reticulum (SER), rough endoplasmic reticulum (RER), and Golgi apparatus, swollen mitochondria in cells showing a high degree of hypertrophy, and an abundance of dense bodies. Following infection, the sinusoidal lumina became dilated and contain a moderate electron-dense precipitate, an abundance of melanomacrophages, lipocytes, and mononuclear cells. There is also a widening of the fenestrae of the sinusoidal endothelium following infection. Many of the changes in hepatocytes and sinusoids following parasite infections closely resemble those observed in hepatocytes in various pathologies and following experimental bile duct ligation and, therefore, are likely a consequence of increased biliary pressure due to bile duct obstruction. 相似文献
12.
cAMP-dependent Cl(-) channel protein (CFTR) and its mRNA are expressed in the secretory portion of human eccrine sweat gland. 总被引:2,自引:0,他引:2
Immunoreactive cystic fibrosis transport regulator (CFTR) proteins in human sweat ducts has been documented but CFTR expression in the secretory coil has remained uncertain. Using monoclonal antibodies (MAbs) against epitopes in the R-domain and C-terminus, we observed the following: Formalin fixation masks the CFTR epitopes but the epitopes are exposed by treatment with urea and heat (antigen retrieval). Pen-Fix fixation preserves CFTR epitopes. The secretory coil also expresses CFTR epitopes for the R-domain and C-terminus. An MAb against C-terminus amino acids 1466-1480 coupled to keyhole limpet hemocyanin (MAb WC) stained dark cells predominantly. Staining by MAbs against the C-terminus was completely blocked by a C-terminus peptide. mRNA for CFTR was amplified by RT-PCR in both the duct and the secretory coil. In situ hybridization for CFTR mRNA after 3SR amplification indicates that mRNA is localized in the dark cells and perhaps also in the clear cell cytoplasm near the secretory coil. mRNA is present in both the luminal and basal duct cells. We conclude that CFTR is expressed equally well in both the duct and the secretory coil, suggesting that cAMP-dependent Cl(-) channels are involved in regulation of sweat secretion and duct absorption. 相似文献
13.
Ishii K Tsubaki T Fujita K Ishigami A Maruyama N Akita M 《Histology and histopathology》2005,20(3):761-768
Senescence Marker Protein-30 (SMP30) is a calcium-regulating protein that decreases in an androgen-independent manner as aging occurs. An enzyme-labeled antibody technique has demonstrated that SMP30 localized to the ducts (granular, intercalated, and striated ducts) of mouse submandibular glands. Immunoelectronmicroscopy demonstrated that the granular duct cells were strongly positive for SMP30, but that pillar cells in the granular duct were negative for the protein. In SMP30-knockout (KO) mice, the granular ducts were smaller in diameter. Swelling of mitochondria in the granular duct cells was observed; however, this phenomenon was not observed in the pillar cells. After administration of alpha-isoproterenol, a beta-adrenergic stimulant, a large numbers of small secretory granules were present in the granular duct cells and an expansion of the rough endoplasmic reticulum in SMP30-wild type (WT) mice; in contrast, little change was observed in SMP30-KO mice. These results suggest that SMP30 may be closely related to a signal transduction pathway in the granular duct cells of submandibular glands. 相似文献
14.
15.
The distribution of S-100 protein and its α- and β-subunits in bovine exocrine glands was studied by indirect immunohistochemistry.
The entire spectrum of salivary glands, glands of the respiratory tract, intestinal glands, male and female genital glands,
and skin glands was examined. S-100 and its β-subunit were identified in most serous secretory cells of mixed salivary glands,
although secretory acini in some serous glands remained unreactive for these antigens. Mucous cells were constantly negative;
mucoid cells were positive in the lacrimal and Harderian gland. The α-subunit of S-100 protein was identified in serous cells
but the staining reaction was faint. Subunits of S-100 showed a characteristic distribution along the excretory duct systems
of compound glands: S-100 and the β-subunit were present in intercalated duct epithelium, while striated duct epithelium stained
for S100-α. Therefore, it is suggested that S100-α is related to resorption and secretion in striated ducts, while S100-β
may govern acinar exocytosis and probably regulates proliferation and differentiation of glandular cells. Differing staining
intensities for S-100 and its subunits in secretory cells of exocrine glands most probably indicate functional differences
with regard to secretory activity and the cell cycle.
Accepted: 11 February 1997 相似文献
16.
Dallai R Lupetti P Giusti F Mercati D Paccagnini E Turillazzi S Beani L Kathirithamby J 《Tissue & cell》2004,36(3):211-220
Nassonow's gland consists of a number of cells with ducts that open on to the ventral surface of the brood canal in the cephalothoracic region of a neotenic female strepsipteran. The structural organization of the gland is reminiscent of the class 3 of the epidermal gland cells as defined by Noirot and Quennedey [Ann. Rev. Entomol. 19 (1974) 61], which consists of secretory and duct forming cells. The ultrastructure of the Nassonow's gland is described in female Xenos vesparum (Rossi) parasitic in the social wasp Polistes dominulus Christ. The large secretory cells are clustered in groups of three to four, rich in smooth endoplasmic reticulum and produce a secretion made up of lipids. In young females, just before mating, the ultrastructure of the cells and their inclusions indicate that they are active. In old-mated females the Nassonow's gland degenerates. Microvilli line an extracellular cavity and there are pores present in the irregularly thick cuticle of the efferent duct. The small duct forming cells, intermingle with epidermal cells, overlap secretory cells and produce a long efferent duct, the cuticle of which becomes thick close to its opening in the brood canal. Nassonow's gland could be the source of a sex pheromone, which might be capable of attracting the free-living male to a permanently endoparasitic female. 相似文献
17.
Sobotník J Kalinová B Cahlíková L Weyda F Ptácek V Valterová I 《Journal of insect physiology》2008,54(1):204-214
The cephalic region of the labial gland in the buff-tailed bumblebee, Bombus terrestris, consists of numerous acini (formed by associated secretory cells and a central lumen) and connecting ducts. Age-dependent changes in secretion production (both qualitative and quantitative) are associated with changes in the amount of rough endoplasmic reticulum (RER), Golgi apparatus, and smooth endoplasmic reticulum (SER). The main secretory organelle is RER in the youngest individuals (pharate, and less-than-a-day old males), Golgi apparatus in 1-day-old males, and SER in males older than 2 days. Secretory cell death starts at 5 days of age, with maximal longevity to 10 days. Pheromone production starts immediately after eclosion, with pheromone quantities increasing until day 7. 2,3-dihydrofarnesol, the main component of the male-marking pheromone, appears in 1-day-old male glands, and reaches a maximum at 7 days of age, when its presence in the gland starts to decrease gradually. Older glands contain compounds not present in young ones. Variation in pheromone quantity and composition are reflected sensitively in the response of the queen antennae. Though queen antennae responded to gland extracts of all ages examined, maximum sensitivity was observed in response to extracts of glands 2-10 days old, while extracts of older glands gradually lose their effectiveness. Both major and minor components of the labial gland secretion extract elicited queen antennal responses suggesting that the pheromone is a multicomponent blend. Age-dependent changes in pheromone production, accumulation and tuning of pheromone activity are all synchronized approximately with male flight from the hive. 相似文献
18.
L T Threadgold 《Experimental parasitology》1975,37(1):117-124
An ultrastructural study of the prostate gland of Fasciola hepatica shows it to be composed of numerous unicellular glands. These gland cells contain an extensive granular endoplasmic reticulum (GER) system parts of which are intimately associated with septum-like invaginations of the plasma membrane extending almost to the nucleus. Also associated with the GER are many Golgi complexes which secrete large electron-lucid carbohydrate-rich secretory vesicles. The secretion passes up the gland ducts along with a very dense granular and fibrillar material. The ducts have a peripheral microtubular skeleton and are tightly bound to the epithelium of the ejaculatory duct by septate desmosomes. Secretory vesicles are stored in the expanded ends of the ducts where they pass through the ejaculatory epithelium and their content is discharged by the bursting of their limiting membrane. 相似文献
19.
《International Journal of Insect Morphology and Embryology》1990,19(2):133-139
A new exocrine gland has been discovered within the sting of the endemic Australian ants of the genera Myrmecia and Nothomyrmecia (Hymenoptera : Formicidae). It consists of approximately 20 secretory cells with their accompanying duct cells, located between the ducts of the venom and Dufour glands in the proximal part of ths sting bulb, hence my suggestion to designate it as the sting bulb gland. Ultrastructural examination reveals the development of both granular and smooth endoplasmic reticulum in the glandular cells, which possibly may indicate the elaboration of a rather complex secretion. Although the function of the gland remains unknown, its exclusive presence in these ants provides another argument for a closer phylogenetic relationship between both genera than is reflected by their actual classification. 相似文献
20.
臭椿茎中分泌道的发育及其组织化学研究 总被引:1,自引:0,他引:1
利用植物解剖学方法研究臭椿茎和叶柄中分泌道的结构、分布和发育过程.结果表明:臭椿茎和叶柄中的分泌道分布于髓的周缘,次生木质部中无分泌道.分泌道是由一层分泌细胞围绕分泌腔而构成,分泌细胞外有1~2层鞘细胞.分泌道以裂生方式形成,其发育过程可分为3个阶段:原始细胞阶段、形成阶段和成熟阶段.在原始细胞阶段,一群原始细胞具浓厚细胞质,细胞核清晰可见;形成阶段,原始细胞的中央细胞间细胞壁中层降解,细胞壁分离,形成腔隙,随着分泌细胞数量的增加,分泌腔体积扩大;成熟阶段的分泌道具有12~16个分泌细胞,1~2层鞘细胞,分泌腔直径为30~50μm.组织化学研究表明,分泌细胞及分泌道内含物中含大量的萜类、多糖和脂类物质.机械创伤能够诱导次生木质部中产生创伤分泌道.臭椿茎中的分泌道和创伤性分泌道在抵御生物和非生物胁迫中起重要作用. 相似文献