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1.
S S Guraya 《The Journal of experimental zoology》1965,160(1):123-135
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J. G. Ferrand 《Invertebrate reproduction & development.》2013,57(3):143-147
Summary Primary lysosomes appear in the oocytes of A. gibbosa at the end of previtellogenesis. The lysosomes fuse with the cisternae of the endoplasmic reticulum and give rise to yolk globules containing acid phosphatase. The yolk globules then grow by fusion. 相似文献
3.
Species differences in localization of cardiac cAMP-phosphodiesterase activity: A cytochemical study
L'udmila Okruhlicová Narcisa Tribulová Ján Styk Anita Eckly Claire Lugnier Ján Slezák 《Molecular and cellular biochemistry》1997,173(1-2):183-188
The localization of the membrane-bound cyclic 3,5-AMP phosphodiesterasein cardiac tissues of both, rat and dog was studied by cytochemical method.40 µm thick slices from glutaraldehyde fixed heart tissue wereincubated in the medium with cAMP as a substrate and Pb ions as a capturemetal of the reaction product. The cAMP-PDE activity in the rat ventriclewas only shown positive on the sarcolemma. Whereas, in canine ventriculartissue the cAMP-PDE activity in cardiomyocytes was shown on the sarcolemma,on the junctional sarcoplasmic reticulum and on subsarcolemmal cisternae.The results confirm differences in the localization of cAMP-PDE in dog andrat heart. 相似文献
4.
Identification of yolk platelet-associated hydrolases in the oocytes of Rhodnius prolixus. 总被引:1,自引:0,他引:1
R H Nussenzveig P L Oliveira H Masuda 《Archives of insect biochemistry and physiology》1992,21(4):253-262
The yolk platelets from Rhodnius prolixus, a blood-sucking bug, are composed mostly of vitellin and here are shown to contain at least two hydrolytic enzymes, a phosphatase and a cathepsin D-like proteinase. Both the proteinase and the phosphatase have an acid pH optimum. No hydrolytic activity was observed under alkaline or neutral conditions. Among several proteinase inhibitors tested, only pepstatin could abolish vitellin breakdown in vitro. The proteinase appears to be bound to the yolk platelet membranes. The phosphatase activity, using p-nitrophenyl phosphate as substrate, was enhanced after disruption of the platelet membrane by Triton X-100. This activity could be inhibited by tartrate but not by p-cloromercuribenzoate. 相似文献
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Possible participation of mitochondria in lipid yolk formation in oocytes of paddlefish and sturgeon 总被引:1,自引:0,他引:1
The ovary of paddlefish and sturgeons (Acipenseriformes) is composed of discrete units: the ovarian nests and ovarian follicles.
The ovarian nests comprise oogonia and numerous early dictyotene oocytes surrounded by somatic prefollicular cells. Each ovarian
follicle consists of a spherical oocyte and a layer of follicular cells situated on a thick basal lamina, encompassed by thecal
cells. The cytoplasm of previtellogenic oocytes is differentiated into two distinct zones: the homogeneous and granular zones.
The homogeneous cytoplasm is organelle-free, whereas the granular cytoplasm contains numerous organelles, including mitochondria
and lipid droplets. We have analyzed the cytoplasm of early dictyotene and previtellogenic oocytes ultrastructurally and histologically.
In the cytoplasm of early dictyotene oocytes, two morphologically different types of mitochondria can be distinguished: (1)
with well-developed cristae and (2) with distorted and fused cristae. In previtellogenic oocytes, the mitochondria of the
second type show various stages of cristae distortion; they contain and release material morphologically similar to that of
lipid droplets and eventually degenerate. This process of mitochondrial transformation is accompanied by an accumulation of
lipid droplets that form a single large accumulation (lipid body) located in the vicinity of the oocyte nucleus (germinal
vesicle). The lipid body eventually disperses in the oocyte center. The possible participation of these mitochondria in the
formation of oocyte lipid droplets is discussed.
This work was supported by funds from the research grant BW/IZ/2005 to M.Ż.
An erratum to this article can be found at http://dx.doi.org/.
An erratum to this article can be found at 相似文献
7.
I. V. Zhuravlev A. M. Dolgorukova A. V. Salamatin V. I. Fisinin 《Russian Journal of Developmental Biology》2005,36(1):1-5
It has been proposed that variations in relative yolk weight in a population of meat-type fowl be used as a model of development of nidicolous and nidifugous birds. During development of the eggs with a high proportion of yolk, an excess of lipids is cleaved at a higher rate and oxidized until day 17 of incubation, while in the embryos developing from the eggs with a low relative yolk weight, amino acids are intensely cleaved during the period preceding the hatching. Significant differences in the body content of cystine were found in 17-day embryos and upon hatching, thus suggesting a delayed activity of the genes encoding keratins in the group corresponding to the seminidicolous type according to the egg content of lipids. These biochemical differences question the widespread concept on the occurrence of dichotomy by the end of embryogenesis and beginning of neonatal growth of nidifugous and nidicolous birds.Translated from Ontogenez, Vol. 36, No. 1, 2005, pp. 3–8.Original Russian Text Copyright © 2005 by Zhuravlev, Dolgorukova, Salamatin, Fisinin. 相似文献
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The development and fine structure of yolk nuclei in the cytoplasm of previtellogenic oocytes were examined by electron microscopy during several stages of oogenesis in the medaka, Oryzias latipes. Shortly after oogenesis starts, oocytes 20-30 microm in diameter have much electron-dense (basophilic) cytoplasm, within which a continuous or discontinuous, irregular ring-shaped lower electron-dense area of flocculent appearance (LF) begins to emerge around the nucleus. The yolk nucleus is first recognized within an LF area as a few fragments of dense granular thread measuring 20-25 nm in width. The threads consist of two rows of very dense granules resembling ribosomes or ribonucleoprotein (RNP)-like particles in size and electron density. These thread-like fragments gradually increase in number and length until they assemble into a compact, spherical mass of complicated networks. Analysis of serial sections suggests that the yolk nucleus is a complicated mass of numerous, small deformed vacuoles composed of a single lamella with double layers of ribosomes or RNP-like granules, rather than a mass of granular threads. When oocytes develop to greater than 100 microm in diameter, the yolk nucleus begins to fragment before dispersing throughout the surrounding cytoplasm, concomitantly with the disappearance of LF areas. At this stage of oogenesis, a restricted region of the granulosa cell layer adjacent to the yolk nucleus becomes somewhat columnar in morphology, fixing the vegetal pole region of the oocyte. 相似文献
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Histochemical observations on lipid changes during oogenesis in the poultry nematode,Ascaridia galli
During oogenesis lipids continue to increase in Ascaridia galli leading to the formation of massive accumulations in larger oocytes. In addition to neutral lipids (triacylglycerols), small amounts of cholesterol and granules containing phospholipids and lipoproteins are seen in the ooplasm of growing and mature oocytes. Lipid inclusions of oogonia which are in the form of coarse granules show a complex structure in young oocytes and form droplets and globules in growing and mature oocytes. Changes in the morphology of the oocytes are accompanied by changes in the distribution of lipids within the oocytes. Localization of lipids in rachis and ovarian epithelium along the length of the ovary is also described. 相似文献
12.
Summary The binding to morphologically normal human retina of eleven biotin- or peroxidase-coupled lectins with different carbohydrate specificities was studied. Eight formalin-fixed and paraffin-embedded eyes were examined. Photoreceptor cells bound Lens culinaris (LCA), wheat germ (WGA), peanut (PNA) and Ricinus communis (RCAI) agglutinins, and concanavalin A (ConA). The outer segment region was labeled more strongly than the inner segment region, and PNA labeled only cones. All these lectins except PNA bound to both plexiform layers, and all but PNA and RCAI to the nuclear layers. Pretreatment with neuraminidase to remove sialic acid resulted in increased binding of RCAI and PNA, which now labeled both rods and cones, and in decreased binding of WGA. Bandeiraea simplicifolia (BSAI), Dolichos biflorus (DBA), soybean (SBA), Ulex europaeus (UEAI), and Lotus tetragonolobus (LTA) agglutinins, as well as pokeweed mitogen (PWM) reacted only with retinal vascular endothelial cells, which were also labeled with the other lectins. The results indicate that -mannose, -glucose, -galactose, N-acetyl-d-glucosamine and N-acetylneuraminic acid are present in glycoconjugates of human neuroretina. 相似文献
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Kress A 《Tissue & cell》1986,18(6):915-935
Runcina is a hermaphroditic opisthobranch mollusc of small size. It produces large eggs, rich in yolk substances, and it is thus ideal for studying the mechanisms of vitellogenesis. The rough endoplasmic reticulum and the Golgi apparatus appear to be involved in early build up of yolk precursors. Endocytotic vesicles carrying yolk proteins, taken up from the haemolymph, dominate yolk formation at a later stage. The findings presented in this study enhance the proposition of a dual pathway of auto- and heterosynthesis in yolk formation. It is not found that the yolk bodies in Runcina acquire lysosomal enzymes in order to digest the perivitelline fluid, as has been described for some pulmonates. The importance of the role played by the follicle cells in oocyte development is discussed. 相似文献
15.
A morphological and cytochemical study of erythrocytes, thrombocytes and leukocytes in four freshwater teleosts 总被引:2,自引:0,他引:2
M. Tavares-Dias 《Journal of fish biology》2006,68(6):1822-1833
Erythrocytes, thrombocytes and leukocytes morphology and cytochemical staining were studied in big head carp Aristichthys nobilis , oscar Astronotus ocellatus , traíra Hoplias malabaricus and lambari Astyanax bimaculatus . Reticulocytes contained a granular material similar to residual RNA following staining with brilliant cresyl blue. Neutrophils, lymphocytes and monocytes were morphologically similar in all the four species. Thrombocytes were present in all the four species and were predominantly fusiform, whereas eosinophils occurred only in A. ocellatus . Aristichthys nobilis contained a leukocyte with unstained granules following Romanowsky-type staining, which stained intensely with periodic acid-Schiff (PAS). Glycogen granules were present in thrombocytes, neutrophils and eosinophils but not in monocytes or lymphocytes. Peroxidase staining was observed in neutrophils of A. ocellatus , H. malabaricus and A. bimaculatus but not in A. nobilis . Monocytes of A. ocellatus , H. malabaricus and A. bimaculatus stained positively for non-specific esterase, whereas those of A. nobilis did not stain. Thrombocytes and leukocytes in all four species were negative for alkaline phosphatase. Neutrophils of A. ocellatus and H. malabaricus may be involved in respiratory burst and may play an important microbicidal role. 相似文献
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Ján Slezák Ludmila Okruhlicová Narcisa Tribulová Wolfgang Schulze Naranjan S. Dhalla 《Molecular and cellular biochemistry》1995,147(1-2):169-172
ATPases of cardiac cells are known to be among the most important enzymes to maintain the fluxes of vital cations by hydrolysis of the terminal high-energy phosphate of ATP. Biochemically the activities of Ca2+-pump ATPase, Ca2+/Mg2+-ecto ATPase, Na+,K+-ATPase and Mg2+-ATPase are determined in homogenates and isolated membranes as well as in myofibrillar and mitochondrial fractions of various purities. Such techniques permit estimation of enzyme activitiesin vitro under optimal conditions without precise enzyme topography. On the other hand, cytochemical methods demonstrate enzyme activityin situ, but not under optimal conditions. Until recently several cytochemical methods have been employed for each enzyme in order to protect its specific activity and precise localization but the results are difficult to interpret. To obtain more consistent data from biochemical and cytochemical point of view, we modified cytochemical methods in which unified conditions for each ATPase were used. The fixative solution (1% paraformaldehyde –0.2% glutaraldehyde in 0.1 M Tris Base buffer, pH 7.4), the same cationic concentrations of basic components in the incubation medium (0.1 M Tris Base, 2mM Pb(NO2)3, 5 mM MgSO4, 5 mM ATP) and selective stimulators or inhibitors were employed. The results reveal improved localization of Ca2+-pump ATPase, Na+–K+ ATPase and Ca2+/Mg2+-ecto ATPase in the cardiac membrane. 相似文献
18.
《Journal of morphology》2017,278(6):768-779
Non‐avian reptiles commonly are assumed to be like birds in their overall patterns of development. However, colubrid corn snakes (Pantherophis guttatus ) have mechanisms of yolk cellularization and processing that are entirely different from the avian pattern. In birds, a vascular “yolk sac” surrounds and digests the liquid yolk. In contrast, in corn snakes, the yolk material is converted into vascularized cords of yolk‐filled cells. In this study, we used stereomicroscopy, histology, and scanning electron microscopy to analyze this unusual developmental pattern in corn snakes. Our observations reveal that the yolk sac cavity is invaded by endodermal cells that proliferate, absorb yolk spheres, and form aggregates of interconnected cells within the liquid yolk mass. As development proceeds, small blood vessels arise from the yolk sac omphalopleure, penetrate into the yolk mass, and become tightly encased in the endodermal cells. The entire vitellus ultimately becomes converted into a mass of vascularized, “spaghetti‐like” strands of yolk‐laden cells. The resulting arrangement allows yolk to be digested intracellularly and yolk products to be transported to the developing embryo. Indirect evidence for this pattern in other species raises the possibility that it is ancestral for squamates and quite possibly Reptilia in general. 相似文献
19.
S. Mahadevan 《Journal of biosciences》1987,11(1-4):299-309
Lipid thermal transition patterns of the very low density lipoproteins in native and variously treated egg yolk plasma and
extracted total very low density lipoproteins lipids have been recorded by differential scanning calorimetry in the temperature
range 220–300 K, after lowering the freeze endotherm of free water in the sample with ethylene glycol.
Three distinguishable patterns of lipid endotherms, designated types 1, 2 and 3 were obtained, respectively, from (i) native
very low density lipoproteins in egg yolk plasma, (ii) freeze damaged very low density lipoproteins in gelled egg yolk plasma
and (iii) extracted total lipids of very low density lipoproteins dispersed in water. Protein-depleted ‘lipid core’ particles
of very low density lipoproteins obtained by exhaustive proteolysis of egg yolk plasma gave type 2 lipid transition pattern
suggesting similarities in its lipid association with that of the freeze damaged very low density lipoproteins. Freezing the
‘lipid cores’ of very low density lipoproteins led to phase separation and gave type 3 lipid transition pattern of water-dispersed,
phase-separated total very low density lipoprotein lipids. Relative heat uptake of native very low density lipoproteins in
egg yolk plasma was about 15% lower than the freeze damaged sample or of the extracted total lipids.
Treatments which prevented aggregation and gelation of very low density lipoproteins in egg yolk plasma during frozen storage,
namely with additives such as glycerol or NaCl, gave subsequent lipid transition pattern intermediate between type 1 and 2,
indicating that while very low density lipoprotein aggregation is prevented, additives do not altogether prevent changes in
lipid association in these particles. 相似文献
20.
Development of the yolk sac of squamate reptiles (lizards and snakes) differs from other amniote lineages in the pattern of growth of extraembryonic mesoderm, which produces a cavity, the yolk cleft, within the yolk. The structure of the yolk cleft and the accompanying isolated yolk mass influence development of the allantois and chorioallantoic membrane. The yolk cleft of viviparous species of the Eugongylus group of scincid lizards is the foundation for an elaborate yolk sac placenta; development of the yolk cleft of oviparous species has not been studied. We used light microscopy to describe the yolk sac and chorioallantoic membrane in a developmental series of an oviparous member of this species group, Oligosoma lichenigerum. Topology of the extraembryonic membranes of late stage embryos differs from viviparous species as a result of differences in development of the yolk sac. The chorioallantoic membrane encircles the egg of O. lichenigerum but is confined to the embryonic hemisphere of the egg in viviparous species. Early development of the yolk cleft is similar for both modes of parity, but in contrast to viviparous species, the yolk cleft of O. lichenigerum is transformed into a tube‐like structure, which fills with cells. The yolk cleft originates as extraembryonic mesoderm is diverted from the periphery of the egg into the yolk sac cavity. As a result, a bilaminar omphalopleure persists over the abembryonic surface of the yolk. The bilaminar omphalopleure is ultimately displaced by intrusion of allantoic mesoderm between ectodermal and endodermal layers. The resulting chorioallantoic membrane has a similar structure but different developmental history to the chorioallantoic membrane of the embryonic hemisphere of the egg. J. Morphol. 2012. © 2012 Wiley Periodicals, Inc. 相似文献