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1.
Ultrastructural aspects of proplastid and chloroplast replicationare described as seen in sections of vegetative cells, sporangia,released spores and sporelings of the red algae Palmaria palmataand Plumaria elegans. Proplastids in apical vegetative cellsshow internal thylakoid formation from peripheral thylakoidsin both species, and proplastid formation by budding from maturechloroplasts has also been observed. Proplastid replicationby fission has been occasionally observed, and genophore divisionin the stroma of proplastids. In vegetative cells, sporangia,spores and sporelings chloroplast formation from mature plastidscan take place by elongation and fission, or by formation ofa discrete group of thylakoids which become pinched off fromthe parent chloroplast, and by irregular expansions of the parentchloroplasts with subsequent multiple fission. Plastid replication, vegetative cells, sporangia, spores, red algae  相似文献   

2.
Recent discovery of humic acid (HA) in the free-living, brown algaPilayella littoralis has prompted a search for HA in other live plants. Marine algaeCodium fragile andMonostoma oxyspermum (greens),Chondrus crispus,Palmaria palmata andPolysiphonia lanosa (reds),Ascophyllum nodosum, Fucus vesiculosus andLaminaria saccharina (browns) andZostera marina (marine angiosperm) were investigated for their HA content. Only the brown algae and the marine angiosperm contained HA, which was extracted by a standard procedure augmented with necessary removal of alginic acid (where applicable). The isolated products were identified as HA by comparison of their analytical data, uv-visible, FTIR,1H NMR spectra and morphologies with those of authentic HA isolated from municipal compost.Authors for correspondence  相似文献   

3.
18S rRNA gene sequences are presented forAhnfeltia plicata, Chondrus crispus, Furcellaria lumbricalis andPalmaria palmata, commercially important marine algae of the North Atlantic. The sequences range from 1765 to 1777 nucleotides in length, with guanine + cytosine content of 50.1% to 52.4%. Sequence divergence between species in different orders was 11.3–12.3%, whereas the variation betweenC. crispus andF. lumbricalis, both from the Gigartinales, was only 3.6%. Based on limited experience with other groups of Rhodophyta, these sequences obtained from single populations are likely to be representative of the species as a whole, with little variation expected among conspecifics regardless of morphological aberration or apparent genetic isolation.NRCC 34824.  相似文献   

4.
ABSTRACT

The state of the art in the field of biosorption using algae as biomass is reviewed. The available data of maximum sorption uptake (qmax) and biomass-metal affinity (b) for Cd2 +, Cu2 +, Ni2 +, Pb2 + and Zn2 + were statistically analyzed using 37 different algae (20 brown algae, 9 red algae and 8 green algae). Metal biosorption research with algae has used mainly brown algae in pursuit of treatments, which improve its sorption uptake. The information available in connection with multimetallic systems is very poor. Values of qmax were close to 1 mmol/g for copper and lead and smaller for the other metals. Metal recovery performance was worse for nickel and zinc, but the number of samples for zinc was very small. All the metals except lead present a similar affinity for brown algae. The difference in the behavior of lead may be due to a different uptake mechanism. Brown algae stand out as very good biosorbents of heavy metals. The best performer for metal biosorption is lead.  相似文献   

5.
Luminescence excitation spectroscopy of the 7F05D0 transition of the Eu(III) complex of 1,4,7,10-tetrakis(carbamoylmethyl)-1,4,7,10-tetraazacyclododecane (TCMC, an amide derivative of DOTA) is used to measure the stability constant of the complex (K). A log K value of 10.6 is obtained for [Eu(TCMC)]3+ at 25 °C and an ionic strength of 0.1 M. Competition experiments with eleven other members of the lanthanide(III) series give stability constants for their complexes with TCMC. An unusual variation in stability is observed for complexes of [Ln(TCMC)]3+ across the lanthanide series with a pronounced optimum for the Sm(III) complex. This variation is quite different from that observed for other Ln(III) macrocyclic complexes, suggesting that the TCMC ligand is uniquely sensitive to Ln(III) ion radius.  相似文献   

6.
The effect of polysaccharidases (κ-carrageenase, β-agarase, xylanase, cellulase) on the protein extraction from three rhodophytes has been studied. The kinetic parameters (apparent V m, apparent K m) and the optimum activity conditions (pH, temperature) of each enzyme were determined by using pure substrates. All the tested enzymes possess Michaelis Menten mechanism with estimated substrate saturating concentrations of 8 000 mg l−1(carrageenan) for κ-carrageenase, 8 000 mg l−1 (agar) for β-agarase, 5000 mg l−1 (xylane) for β-xylanase and 6 000 mg l−1 (carboxymethylcellulose) for cellulase. The optimum activity conditions are pH 6.5–6.8 at 45°C for carrageenase, pH 6–6.5 at 55°C for agarase, pH 5 at 55°C for xylanase and pH 3.8 at 50°C for cellulose. Different alga/enzymes couples (κ-carrageenase/Chondrus crispus, β-agarase/Gracilaria verrucosa, β-xylanase/Palmaria palmata) were tested under the optimum activity conditions. Alga/cellulase + specific enzyme (e.g. Chondrus crispus/carrageenase + cellulase) systems were also studied at the optimum activity conditions of a specific enzyme (e.g. carageenase). The use of the only cellulose was also tested on each alga. Except for Palmaria palmata, the highest protein yields were observed with the procedures using cellulase coupled with carrageenase or agarase for an incubation period limited to 2 h. The Chondrus crispus/carrageenase + cellulose and Gracilaria verrucosa/agarase + cellulase systems gave ten-fold and three-fold improvements, respectively, in protein extraction yield as compared to the enzyme-free blank procedure. The combined action of xylanase and cellulose on protein extraction from Palmaria palmata does not significantly improve protein yield. The best overall protein yield for P. palmata is for P. palmata/xylanase with a 14-h incubation time. This study shows the interest in the use of a polysaccharidase mixture for improving protein extractibility from certain rhodophytes. This biotechnology approach, adapted from procedures for protoplast production or enzymatic liquefaction of higher plants, could be tested as an alternative method to obtain proteins from seaweeds of nutritional interest.  相似文献   

7.
The electrochemical behavior and thermodynamic properties of Ln(III) (Ln = Eu, Sm, Dy, Nd) were studied in 1-butyl-3-methylimidazolium bromide ionic liquid (BmimBr) at a glassy carbon (GC) electrode in the range of 293–338 K. The electrode reaction of Eu(III) was found to be quasi-reversible by the cyclic voltammetry, the reactions of the other three lanthanide ions were regarded as irreversible systems. An increase of the current intensity was obtained with the temperature increase. At 293 K, the cathodic peak potentials of −0.893 V (Eu(III)), −0.596 V (Sm(III)), −0.637 V (Dy(III)) and −0.641 V (Nd(III)) were found, respectively, to be assigned to the reduction of Ln(III) to Ln(II). The diffusion coefficients (D o), the transfer coefficients (α) of Ln(III) (Ln = Eu, Sm, Dy, Nd) and the charge transfer rate constants (k s) of Eu(III) were estimated. The apparent standard potential (E 0*) and the thermodynamic properties of the reduction of Eu(III) to Eu(II) were also investigated.  相似文献   

8.
The hexavalent chromium Cr(VI) poses a threat as a hazardous metal and its removal from aquatic environments through biosorption has gained attention as a viable technology of bioremediation. We evaluated the potential use of three green algae (Cladophora glomerata, Enteromorpha intestinalis and Microspora amoena) dry biomass as a biosorbent to remove Cr(VI) from aqueous solutions. The adsorption capacity of the biomass was determined using batch experiments. The adsorption capacity appeared to depend on the pH. The optimum pH with the acid-treated biomass for Cr(VI) biosorption was found to be 2.0 at a constant temperature, 45?°C. Among the three genera studied, C. glomerata recorded a maximum of 66.6% removal from the batch process using 1.0?g dried algal cells/100?ml aqueous solution containing an initial concentration of 20?mg/L chromium at 45?°C and pH 2.0 for 60?min of contact time. Langmuir and Freundlich isotherm equations fitted to the equilibrium data, Freundlich was the better model. Our study showed that C. glomerata dry biomass is a suitable candidate to remove Cr(VI) from aqueous solutions.  相似文献   

9.
Summary

216 species of diatoms have been found epiphytic on common intertidal marine algae from Great Cumbrae Island, Firth of Clyde, Scotland. Green and red algae had larger epiphytic floras than brown algae. Cocconeis spp. (especially C. scutellum var. scutellum) were common and abundant, and the endophytic Navicula endophytica was found in large numbers in fucoid algae. Similar epiphytic floras were observed on Cladophora rupestris, Polysiphonia lanosa, Gigartina stellata, and Palmaria palmata, but that on Enteromorpha intestinalis was quite distinct.  相似文献   

10.
Yang F  Liu H  Qu J  Paul Chen J 《Bioresource technology》2011,102(3):2821-2828
A new biosorbent - Sargassum sp. encapsulated with epichlorohydrin (ECH) cross-linked chitosan (CS) was investigated for nickel ions removal. The prepared biosorbent with Sargassum sp. to cross-linked chitosan of 3 (weight ratio) had the highest sorption capacity. The biosorption kinetics can be well fitted by the diffusion-controlled model. The organic leaching of CS was 77-88% less than that of algae at different pH. The biosorption capacity of nickel on CS was much higher than that of cross-linked chitosan (CLC) bead and lower than that of raw algae due to encapsulation. In addition, the reusability of CS was further evaluated and confirmed through five adsorption-desorption cycles. Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS) analysis demonstrated that the nickel ions sequestration mechanism included ion exchange and nickel complexation with the carboxyl, amino, alcoholic and ether groups in CS.  相似文献   

11.
In order to find new sources of arachidonic (AA) and eicosapentaenoic (EPA) acids, the composition of fatty acids was studied and lipid concentrations were determined in the thalluses of 32 species of red algae from Peter the Great Bay, Sea of Japan. The greatest level of EPA and a small concentration of AA were registered in the thalluses of Corallina pilulifera, Palmaria stenogona, Halosaccion yendoi, and Laurencia nipponica. Taking into consideration the level of the lipid concentrations in the algae, as well as their biomass and frequency of occurrence, the algae C. pilulifera, P. stenogona, L. nipponica, and Polysiphonia morrowii may be of interest as potential sources of EPA. Among the examined algae, only Gracilaria verrucosa showed a high level of AA.  相似文献   

12.
Cultures of the plankton diatoms Skeletonema costatum (Greville) Cleve, Thalassiosira nordenskioeldii Cleve, and Lauderia annulata Cleve were grown in sea-water samples collected in the inner Oslofjord on 11 occasions between February, 1976 and January, 1977. The growth experiments were carried out in silicone-coated culture flasks, with nitrate, phosphate, and silicate added in excess, and under standardized conditions of a temperature of 10 °C and a saturating light intensity. Growth rates, as measured during exponential growth over a period of 5–6 days, were, with very few exceptions, close to the maximum rates obtained in a complete medium. The addition of ethylenediaminetetra-acetic acid (EDTA) to the water samples did not lead to improved growth, nor did EDTA have an effect on the initial lag phase. The lack of a seasonal variation in the observed growth responses was in marked contrast to the large fluctuations in the phytoplankton populations in the fjord. These findings give no support to the hypothesis that species successions among marine plankton diatoms are influenced by organic chelators or other extracellular substances produced by planktonic algae.  相似文献   

13.
Mesoporous Ln(III)-TiO2 (Ln = Tb, Eu, Sm) nanomaterials composites have been successfully synthesized by using sol-gel technique.XRD pattern, FT-IR, Raman spectra, and SEM were used to characterize the Ln(III)-TiO2 nanomaterials. The prepared lanthanide doped TiO2 nanomaterials have anatase phase and exhibit Ti-O-Ln bond. The absorption spectra of all prepared samples reflect the increasing photoresponse of doped samples to visible light over pure TiO2. Surface area is remarkably increased due to lanthanide ion-doping.Two newly prepared Ln(III)-TiO2 (Ln = Eu, Sm) luminescent nanomaterials exhibit enhanced pure red or orange light emission due to energy transfer from host TiO2 to guest Eu(III) or Sm(III), respectively.In addition, the commercially available textile dye Remazol Red RB-133 degradation was used as a probe reaction to determine the efficiency of the Ln(III)-TiO2 photocatalysts. The Ln(III) doping brought about remarkable improvement in the photoactivity over pure TiO2.  相似文献   

14.
The level of auxin-like compounds extracted from the seaweed Chondrus crispus L. (Lyn.) is reported. Their presence before and after alkaline hydrolysis is studied to confirm that the bound auxin-like substances are present in algae in greater concentration than the free form.  相似文献   

15.
On the basis of experimental research on the influence of crude oil on basic functional characteristics (growing speed, photosynthesis, and breadth) of macroalgae of the Barents Sea (Laminaria saccharina, Fucus vesiculosus, Ascophyllum nodosum, Porphyra umbilicalis, Palmaria palmata, and Enteromorpha prolifera) it was shown that kelp are more resistant to the influence of oil carbohydrates while red and green algae are less resistant. Under the short-term influence of oil toxins photosynthesis is depressed and respiration increases, under long-term influence the rate of growth is reduced.  相似文献   

16.
Using five species of bacteria as the test organisms, 151 species of British marine algae have been screened for the production of antibiotics. Of these, Asparagopsis armata, Bonnemaisonia asparagoides, Bonnemaisonia hamifera, Chondrus crispus, Dilsea carnosa, Gloiosiphonia capillaris, Sphondylothamnion multifidum, Desmarestia aculeata, Desmarestia ligulata, Laminaria digitata, Dictyopteris membranacea, Dictyota dichotoma, Halidrys siliquosa and most members of the family Rhodomelaceae appear to possess outstanding antibacterial properties. Although the production of antibiotics would appear to be a characteristic of several families, it has not been possible to establish any major correlation between taxonomy and antibiotic production. In the case of two closely related and morphologically similar species, Chondrus crispus and Gigartina stellata, the former possesses considerable degrees of antimicrobial activity whilst the latter exhibits no such activity. The results also indicate that the production of antibiotics by the algae is affected by the season of the year.  相似文献   

17.
Four different fluorescent dyes Acridine Orange (AO), Fluorescein Diacetate (FDA), Calcofluor White M2R (CW), and Europium (III) Thenoyltrifluoroacetonate, 3-Hydrate (Eu(TTA)3) were tested to distinguish rapidly between dead and living sclerotia from Sclerotinia trifoliorum. FDA, 0.01% diluted in phosphate buffer, had the shortest staining period and was easy to handle. It is especially suitable to test large numbers of sclerotia for viability. Furthermore the preparation of the samples for the staining procedure is described in this paper.  相似文献   

18.
In this work, the properties of marine algae Gelidium, algal waste from agar extraction industry and a composite material were investigated for cadmium(II) biosorption. Equilibrium experiments were performed at three pH values (4, 5.3 and 6.5). Equilibrium data were well described by the Langmuir and Langmuir–Freundlich isotherms. Two models predicting the pH influence in the cadmium biosorption (discrete and continuous models) have been developed in order to better describe the equilibrium. The continuous model also considers a heterogeneous distribution of carboxylic groups, determined by potentiometric titration. The results of batch kinetic experiments performed at different pH values were well fitted by two mass transfer models and the homogeneous diffusion coefficients for the cadmium ions inside the biosorbent were obtained. Continuous stirred tank reactor (CSTR) and packed bed column configurations were also examined for the biosorption of cadmium ions. A strong acid (0.1 M HNO3) was used as eluant to regenerate the biosorbents in the column. Several mass transfer models were applied with success to describe the biosorption process in batch mode, CSTR and fixed bed column.  相似文献   

19.
The high cost of aeration needed to tumble culture macroalgae is a limiting factor for integration with land-based finfish culture. Toward reducing this electricity cost, we compared intermittent aeration (16 h on:8 h off) with continuous aeration (24 h on) on the productivity of two strains of Chondrus crispus (Basin Head and Charlesville) and Palmaria palmata from Atlantic Canada between May and June 2011. Algal fronds were cultured under a 16:8-h light/dark photoperiod in 50-L tanks supplied with finfish effluent (49 μmol L?1 of ammonium and 11 μmol L?1 of phosphate) at a mean water flow rate of 0.4 L min?1. Nitrogen (N) influx was 1.8 gN m?2 day?1, and phosphorus (P) influx was 0.9 gP m?2 day?1, with uptake rates ranging from 0.02 to 2.4 gN m?2 day?1 and ?0.2 to 0.4 gP m?2 day?1. On average, the macroalgae culture system (algae and biofilms) removed 1.0 gN m?2 day?1 (51.9 %). The growth of macroalgae (pooled across treatment and strain) ranged from 0.5 to 1.6 % day?1, which accounted for a yield of 2.2 to 5.4 g DW m?2 day?1. Switching off aeration at night improved the growth rate of Basin Head Chondrus by 146 % and had no effect on growth rate or nitrogen and carbon removal by P. palmata and Charlesville Chondrus. Growth and yield of Basin Head Chondrus under intermittent aeration were over two times greater than both Charlesville Chondrus treatments.  相似文献   

20.
《Process Biochemistry》2007,42(11):1521-1529
The biosorption data of hexavalent chromium by marine brown algae Cystoseira indica, which was chemically modified by crosslinking with epichlorohydrin (CB1, CB2), or oxidized by potassium permanganate (CB3), or only washed with distilled water (RB), has been used for kinetic studies based on fractional power, Elovich, pseudo-first order and pseudo-second order rate expressions. Five three parameter biosorption isotherm models, viz. Redlich–Peterson, Sips, Khan, Radke–Prausnitz and Toth are tested for their applicability apart from 6 two-parameter models. Non-linear curve fitting procedure was adopted for fitting the kinetic as well as equilibrium data in the kinetic and isotherm models and for the determination of parameters. The time-dependent Cr(VI) biosorption data were well-described by pseudo-second-order kinetic model. The intraparticle diffusion study revealed that film diffusion might be involved in Cr(VI) biosorption in the present case. Among the two-parameter models, the Langmuir model produces the best fit, while, among the three-parameter models, the best fit is produced by the Khan model, for the biosorption of Cr(VI) on all the four biosorbents studied.  相似文献   

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