Compartmentation in Vicia faba Leaves: II. Kinetics of C-Sucrose Redistribution among Individual Tissues following Pulse Labeling

Leaflets of Vicia faba L. were pulse labeled with ¹⁴CO₂ and the kinetics of ¹⁴C-sucrose redistribution among individual tissues was followed. Sucrose specific activity in the whole leaf peaked about 15 minutes after labeling and declined with a half-time of about 80 minutes. In one experiment, leaflet discs taken at various times during the ¹²CO₂ chase were quick frozen, freeze-substituted, and embedded in plastic. The tissue was sectioned paradermally and sections of palisade parenchyma, of spongy parenchyma, and of spongy parenchyma that contained veins were collected. Water extracts from these sections were assayed for sucrose specific activity. Sucrose specific activity in the palisade parenchyma was higher than that of the spongy parenchyma and reached a maximum in both tissues 9 to 15 minutes after labeling. Sucrose specific activity initially declined rapidly in the palisade parenchyma followed by a period during which little or no loss occurred. Sucrose specific activity in sections containing veins peaked at 15 minutes with a maximum value substantially higher than either mesophyll tissue, indicating that recently synthesized sucrose was preferentially exported from the mesophyll. Decline of activity in these sections containing veins continued for the remainder of the experiment. Sucrose specific activity in lower epidermal peels peaked several minutes after that of the whole leaflet and remained lower. Sucrose specific activity in upper epidermal peels was variable (probably due to contamination), but the limited data suggest that the sucrose specific activity there reached somewhat higher values than those of the lower epidermis. The experiments indicate that each leaf tissue contains a kinetically identifiable sucrose pool (which we refer to as “histological compartmentation”), and that further compartmentation may occur at the intracellular level. A simulation of leaf sucrose compartmentation is presented.     

Carbon Flow and Metabolic Specialization in the Tissue Layers of the Crassulacean Acid Metabolism Plant, Peperomia camptotricha

Leaves of Peperomia camptotricha contain three distinct upper tissue layers and a one-cell thick lower epidermis. Light and dark CO₂ fixation rates and the activity of ribulose bisphosphate carboxylase/oxygenase and several C₄ enzymes were determined in the three distinct tissue layers. The majority of the C₄ enzyme activity and dark CO₂ fixation was associated with the spongy mesophyll, including the lower epidermis; and the least activity was found in the median palisade mesophyll. In contrast, the majority of the C₃ activity, that is ribulose bisphosphate carboxylase/oxygenase and light CO₂ fixation, was located in the palisade mesophyll. In addition, the diurnal flux in titratable acidity was greatest in the spongy mesophyll and lowest in the palisade mesophyll. The spatial separation of the C₃ and C₄ phases of carbon fixation in P. camptotricha suggests that this Crassulacean acid metabolism plant may have low photorespiratory rates when it exhibits daytime gas exchange (that is, when it is well watered). The results also indicate that this plant may be on an evolutionary path between a true Crassulacean acid metabolism plant and a true C₄ plant.     

葛(豆科)叶的解剖学研究

利用光学显微镜和扫描电镜观察了葛(Pueraria lobata)叶的解剖学特征。结果表明,葛叶片的上、下表皮都只有一层表皮细胞,上表皮比下表皮厚。上、下表皮都有腺毛和非腺毛。气孔主要分布在下表皮,下表皮的气孔密度为(261±17)mm-2,上表皮只有(6±3)mm-2。叶肉由两层栅栏组织细胞和一层海绵组织细胞构成。叶肉细胞中有丰富的叶绿体。在栅栏组织和海绵组织之间有一层平行于叶脉的薄壁细胞。叶脉中含有大量的草酸钙晶体。葛叶的这些形态特征与其喜阳、耐旱的特点相适应。     

Structural Adaptation of the Leaf Mesophyll to Shading

Structural characteristics of the mesophyll were studied in five boreal grass species experiencing a wide range of light and water supply conditions. Quantitative indices of the palisade and spongy mesophyll tissues (cell and chloroplast sizes, the number of chloroplasts per cell, the total cell and chloroplast surface area per unit leaf surface area) were determined in leaves of each of the species. The cell surface area and the cell volume in spongy mesophyll were determined with a novel method based on stereological analysis of cell projections. An important role of spongy parenchyma in the photosynthetic apparatus was demonstrated. In leaves of the species studied, the spongy parenchyma constituted about 50% of the total volume and 40% of the total surface area of mesophyll cells. The proportion of the palisade to spongy mesophyll tissues varied with plant species and growth conditions. In a xerophyte Genista tinctoria, the total cell volume, cell abundance, and the total surface area of cells and chloroplasts were 30–40% larger in the palisade than in the spongy mesophyll. In contrast, in a shade-loving species Veronica chamaedris, the spongy mesophyll was 1.5–2 times more developed than the palisade mesophyll. In mesophyte species grown under high light conditions, the cell abundance and the total cell surface area were 10–20% greater in the palisade mesophyll than in the spongy parenchyma. In shaded habitats, these indices were similar in the palisade and spongy mesophyll or were 10–20% lower in the palisade mesophyll. In mesophytes, CO₂ conductance of the spongy mesophyll accounted for about 50% of the total mesophyll conductance, as calculated from the structural characteristics, with the mesophyll CO₂ conductance increasing with leaf shading.     

Photosynthetic Carbon Metabolism in the Palisade Parenchyma and Spongy Parenchyma of Vicia faba L

Palisade parenchyma cells and spongy parenchyma cells were isolated separately from Vicia faba L. leaflets. Extracts of the cell isolates were assayed for several enzymes involved in CO₂ fixation and photorespiration. When compared on a chlorophyll basis, the levels of enzyme activities either were equal in the different cell types or were greater in the spongy parenchyma; this difference is a reflection, perhaps, of the higher protein-chlorophyll ratio in the latter tissue. The distribution of radioactivity in the products of photosynthesis by each cell type was the same at various times after exposure to NaH¹⁴CO₃, and the kinetics of ¹⁴C incorporation into these compounds was similar. However, a larger percentage of radioactivity was incorporated by the cell isolates into the 80% ethanol-insoluble fraction and correspondingly less into the neutral fraction as compared to whole leaf. It was concluded that photosynthetic CO₂ fixation is similar in the different mesophyll tissues from which these cells were derived.     

家蚕消化管内桑叶的银染法研究

通过银染法对家蚕整体染色,结果表明:家蚕消化管内的桑叶由叶表皮、叶肉和叶脉组成。叶表皮包括上表皮和下表皮。上表皮细胞可分为三种:钟乳体细胞、绿色表皮细胞和黄色表皮细胞;下表皮内含有气孔;叶肉组织内含有晶体,其中海绵组织内的最多。家蚕消化管由前向后可分为前肠、中肠和后肠,由外向内依次为肌层、底膜、上皮细胞层、内膜。中肠最为发达,其发达的上皮细胞向内表面突起形成许多大的指突形皱褶;上皮细胞层内有圆筒形细胞、杯形细胞两种细胞,两者在形状、功能以及嗜银性等方面有所差异。家蚕消化管对桑叶不同组织的消化吸收效率有差异,上表皮吸收效率最高,下表皮和栅栏组织次之,最低的是海绵组织。采用动物细胞染色方法对植物细胞进行染色,并与常规植物学染色方法进行了比较;依据细胞嗜银性的不同,可将桑叶的上表皮细胞分为两种亚型。     

从幼苗形态学特征探讨红豆杉科各属间的系统演化

本文根据幼苗的形态学特征,尤其是子叶的结构、说明在红豆杉科中最原始的属是榧树属,其幼苗为留土萌发,植株粗壮;子叶肥厚,有吸收功能,叶肉不分化,其细胞中充满淀粉粒：维管束在子叶的上部或顶部为单束2条,下部合并成双束1条。其次是穗花杉属,幼苗为出土萌发,植株粗壮,下胚轴的下部肥厚;子叶稍肉质,叶肉不分化,质体淡绿,有一定的光合功能,但主要是积累淀粉,维管束双束1条,在子叶的顶端趋于分裂。最进化的是红豆杉属和白豆杉属,可能还有澳洲红豆杉属,其幼苗为出土萌发,植株纤细;子叶薄,叶状,叶肉分化成栅栏和海绵组织,维管束单束1条。本科中族的划分和属的系统排列,从原始到进化,应该是：族1．榧树族(1．榧树属);族2．穗花杉族(2．穗花杉属);族3．红豆杉族(3．澳洲红豆杉属,4．红豆杉属,5．白豆杉属)。     

Paclobutrazol enhances photosynthesis and ajmalicine production in Catharanthus roseus

An investigation was carried out with paclobutrazol (PBZ) in order to study its effect on the photosynthetic and anatomical characteristics of Catharanthus roseus (L.) G. Don. The chlorophyll content and related parameters like net photosynthetic rate, transpiration rate and internal CO₂ concentration were measured from control and treated plant samples. The anatomical characteristics, viz., leaf thickness, epidermis and cuticle, palisade layer, spongy layer and vascular bundle were studied. The root alkaloid ajmalicine was extracted and estimated from both treated and control plant roots. Photosynthetic pigment increased under PBZ treatment. PBZ treatment increased the net photosynthetic rate and internal CO₂ concentration, but reduced transpiration. PBZ treatment increased the thickness of the leaf, epidermis and cuticle, palisade layer and spongy layer but reduced the diameter of xylem vessels significantly when compared to control, however the phloem elements had shown an increased diameter as compared to control in the PBZ treated plants. There was a significant enhancement in ajmalicine content under PBZ treatment when compared to control plants. The results suggested that the application of PBZ have significant effects on photosynthetic and anatomical responses thus can be used for improving productivity in medicinal plants.     

湖南安息香属植物的叶片比较解剖学研究

利用光学显微镜和扫描电镜对湖南产安息香属10种植物叶表皮微形态和解剖结构进行了观察和测量。结果表明:(1)表皮均由单层细胞构成,细胞形状为多边形和无规则形,垂周壁式样为平直、弓形、波状;(2)气孔器大小及密度在种间的差异明显;(3)气孔外拱盖内缘主要为波状和浅波状,气孔器外围角质纹饰以越南安息香的鸟巢状和栓叶安息香的碗状为显著区别特征;(4)老鸹铃、垂珠花和白花龙的气孔器保卫细胞两极有"T"形加厚;(5)栓叶安息香和大果安息香叶上表皮由大型细胞组成;(6)叶肉分化为栅栏组织和海绵组织,二者的厚度及比值在种间有明显的差异,其中越南安息香栅栏组织分化为2层;(7)大果安息香和芬芳安息香的主脉维管组织构成一圈封闭的环状结构,而其他种则为开放的半圆形结构;(8)叶片及主脉的厚度在种间差异明显。安息香属植物的表皮微形态及解剖结构特征比较稳定,可作为种间鉴定和分类的重要依据。     

Leaf responses to iron nutrition and low cadmium in peanut: anatomical properties in relation to gas exchange

Aims

This study evaluated how iron nutrition affect leaf anatomical and photosynthetic responses to low cadmium and its accumulation in peanut plants.

Methods

Seedlings were treated with Cd (0 and 0.2 μM CdCl₂) and Fe (0, 10, 25, 50 or 100 μM EDTA-Na₂Fe) in hydroponic culture.

Results

Cadmium accumulation is highest in Fe-deficient plants, and dramatically decreased with increasing Fe supply. The biomass, gas exchange, and reflectance indices were highest at 25 μM Fe²⁺ treatments, indicating the concentration is favorable for the growth of peanut plants. Both Fe deficiency and Cd exposure impair photosynthesis and reduce reflectance indices. However, they show different effects on leaf anatomical traits. Fe deficiency induces more and smaller stomata in the leaf surface, but does not affect the inner structure. Low Cd results in a thicker lamina with smaller stomata, thicker palisade and spongy tissues, and lower palisade to spongy thickness ratio. The stomatal length and length/width ratio in the upper epidermis, spongy tissue thickness, and palisade to spongy thickness ratio were closely correlated with net photosynthetic rate, stomatal conductance, and transpiration rate.

Conclusions

Cd accumulation rather than Fe deficiency alters leaf anatomy that may increase water use efficiency but inhibit photosynthesis.     

沙棘雌雄株叶片解剖结构比较研究

为了研究沙棘雌、雄株叶片的第二性征,本文采用石蜡切片法观察了沙棘雌、雄株叶片结构的差异。结果表明：（1）沙棘雌、雄株叶片均由表皮、叶肉和叶脉3部分组成,表皮均由1层细胞构成,表皮毛发达,上表皮有拟泡状细胞;叶肉栅栏组织与海绵组织分化明显。（2）雌株上表皮具更多的拟泡状细胞,其主脉韧皮部薄壁细胞及其下方的一些薄壁细胞含较多的后含物,下表皮的表皮毛更浓密;而雄株的叶片厚度、叶片上表皮厚度、栅栏组织厚度、栅栏组织厚度/海绵组织厚度均显著大于雌株,且其主脉维管束更发达。结果表明,沙棘雌雄株叶片解剖结构存在明显差异,这些差异是第二性征的表现,也是沙棘长期进化中形成的稳健的适应策略,可能有利于该物种的繁衍。     

Leafminers help us understand leaf hydraulic design

Leaf hydraulics of Aesculus hippocastanum L. were measured over the growing season and during extensive leaf mining by the larvae of an invasive moth (Cameraria ohridella Deschka et Dimic) that specifically destroy the palisade tissue. Leaves showed seasonal changes in hydraulic resistance (R_lamina) which were related to ontogeny. After leaf expansion was complete, the hydraulic resistance of leaves and the partitioning of resistances between vascular and extra‐vascular compartments remained unchanged despite extensive disruption of the palisade by leafminers (up to 50%). This finding suggests that water flow from the petiole to the evaporation sites might not directly involve the palisade cells. The analysis of the temperature dependence of R_lamina in terms of Q₁₀ revealed that at least one transmembrane step was involved in water transport outside the leaf vasculature. Anatomical analysis suggested that this symplastic step may be located at the bundle sheath where the apoplast is interrupted by hydrophobic thickening of cell walls. Our findings offer some support to the view of a compartmentalization of leaves into well‐organized water pools so that the transpiration stream would involve veins, bundle sheath and spongy parenchyma, while the palisade tissue would be largely by‐passed with the possible advantage of protecting cells from short‐term fluctuations in water status.     

自然越冬期间茶树叶片生理生化指标和解剖结构的变化

为探讨自然越冬条件下茶树〔Camellia sinensis(Linn.)O.Ktze.〕叶片的生理生化指标和解剖结构变化,以种植于南京的茶树品种'龙井长叶'('Longjingchangye')的10年生植株为研究对象,对自然越冬期间(2012年11月15日至2013年3月31日)其叶片的叶绿素、可溶性蛋白质、可溶性糖、游离脯氨酸和丙二醛(MDA)含量,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性,以及上表皮、下表皮、栅栏组织和海绵组织厚度及上表皮厚度与海绵组织厚度的比值进行了比较分析.结果表明:自然越冬期间,叶片的叶绿素含量随时间推移呈现先降低后升高的变化趋势,而叶片的可溶性蛋白质、可溶性糖、游离脯氨酸和MDA含量以及SOD、CAT和POD活性则总体上呈现先升高后降低的变化趋势,并且均在2013年1月15日达到其各自的最低值或最高值.与入冬前相比,越冬后叶片的叶绿素和可溶性糖含量显著降低,MDA含量略降低,可溶性蛋白质含量显著升高,游离脯氨酸含量及SOD、CAT和POD活性均略升高.自然越冬期间,叶片的上表皮、下表皮、栅栏组织和海绵组织厚度随时间推移总体上呈现先降低后升高的变化趋势,上表皮和栅栏组织厚度在2013年2月15日达到最低值,而下表皮和海绵组织厚度则在2013年1月30日达到最低值;上表皮厚度与海绵组织厚度的比值呈波动变化趋势.与入冬前相比,入冬后叶片的上表皮、下表皮、栅栏组织和海绵组织厚度均显著降低,而上表皮厚度与海绵组织厚度的比值略降低.研究结果显示:自然越冬条件下,茶树具有一定的耐寒能力,为了减轻低温对其机体的伤害可通过增加可溶性蛋白质、可溶性糖和游离脯氨酸合成及提高相关抗氧化酶活性来调解越冬期间的代谢平衡.     

Photosynthetic and anatomic responses of peanut leaves to cadmium stress

Cadmium (Cd) treatments caused an inhibition in the net photosynthetic rate (P _N) of peanut (Arachis hypogaea) plants, due to the reduction of stomatal conductance (g _s) and photosynthetic pigment contents, as well as the alteration in leaf structure. The decrease of the transpiration rate and g _s might result from the Cd-induced xerophyte anatomic features of leaves (i.e. thick lamina, upper epidermis, palisade mesophyll, high palisade to spongy thickness ratio, as well as abundant and small stomata). The decline of P _N was independent of the impairment in photosystem 2.     

组织培养条件下喜树叶片细胞壁酸性降解的pH值观察

喜树叶片外植体经组织培养第13 d和23 d后进行解剖学观察,同时比较正常叶片的解剖学特征,发现在pH值为5.8的酸性培养基中,喜树叶片外植体中的海绵组织和栅栏组织等薄壁细胞相继发生明显的细胞壁降解,而表皮细胞发生较微弱的细胞壁降解现象;进行组织培养前,正常叶片的各类细胞未观察到细胞壁降解现象发生。应用BCECF-AM pH荧光探标记并采用激光共聚焦在480 nm波长下进行pH值测定发现,喜树叶片外植体经组织培养第13 d和第23 d后的海绵组织和栅栏组织等薄壁细胞部位的pH值均为5.2,但表皮细胞部位的pH值则为5.7~5.8,而正常叶片各类细胞的pH值平均为5.7。这说明, pH值为5.8的酸性培养基和喜树叶片薄壁细胞内的酸性成分自泌可能共同诱导了其细胞壁的酸性降解。     

龙苍沟国家森林公园7种花楸属植物的叶解剖特征及其环境适应性

采用石蜡切片法对四川省龙苍沟国家森林公园内7种花楸属（Sorbus）植物的叶解剖特征进行研究,探究其结构特征与生境的相关性。结果显示：7种植物的叶片均为典型的背腹叶;叶片厚度介于108.16~208.21 μm,种间差异极显著（P<0.01）;上表皮厚度均大于下表皮厚度,且复叶物种的下表皮细胞均有乳突;栅栏组织由1~2层细胞构成,仅多对西康花楸（S. prattii var. aestivalis）的栅海比（栅栏组织与海绵组织的厚度比）为1.93,其余6种植物的栅海比均小于1;中脉维管束均呈心型,为典型的外韧型维管束,种间中脉突起度存在极显著差异（P<0.01）。各解剖结构中,上、下表皮可塑性最大,在生境中具有较强的潜在适应能力;中脉可塑性最小,整体结构较为稳定。栅栏组织、海绵组织和中脉组织是7种植物中种间差异最大的解剖结构。叶解剖结构与生境因子的相关性分析表明,栅栏组织厚度、栅海比和紧密度与年降水量、最暖季降水和海拔正相关（P<0.05）,与季节性温差负相关（P<0.05）;中脉直径和突起度与季节性温差呈正相关（P<0.01）,与年降水量、最暖季降水和海拔正相关负相关（P<0.05）。叶解剖结构性状的适应性变化,体现了7种花楸属植物在龙苍沟国家森林公园的生存策略。     

Leaf anatomy during leaf development of photoautotrophically <Emphasis Type="Italic">in vitro</Emphasis>-grown tobacco plants as affected by growth irradiance

Tobacco (Nicotiana tabacum L.) plants were cultured in vitro photoautotrophically at three levels of irradiance (PAR 400–700 nm): low (LI, 60 μmol m⁻² s⁻¹), middle (MI, 180 μmol m⁻² s⁻¹) and high (HI, 270 μmol m⁻² s⁻¹). Anatomy of the fourth leaf from bottom was followed during leaf development. In HI and MI plants, leaf area expansion started earlier as compared to LI plants, and both HI and MI plants developed some adaptations of sun species: leaves were thicker with higher proportion of palisade parenchyma to spongy parenchyma tissue. Furthermore, in HI and MI plants palisade and spongy parenchyma cells were larger and relative abundance of chloroplasts in parenchyma cells measured as chloroplasts cross-sectional area in the cell was lower than in LI plants. During leaf growth, chloroplasts crosssectional area in both palisade and spongy parenchyma cells in all treatments considerably decreased and finally it occupied only about 5 to 8 % of the cell cross-sectional area. Thus, leaf anatomy of photoautotrophically in vitro cultured plants showed a similar response to growth irradiance as in vivo grown plants, however, the formation of chloroplasts and therefore of photosynthetic apparatus was strongly impaired.     

Effects of elevated CO<Subscript>2</Subscript> applied to potato roots on the anatomy and ultrastructure of leaves

The root system of potato (Solanum tuberosum L. cv. Favorita) plants was treated with different O₂ and CO₂ concentrations for 35 d in aeroponic culture. Under 21 or 5 % O₂ in the root zones, the thickness of leaves and palisade parenchyma significantly increased at 3 600 μmol(CO₂) mol⁻¹ in the root zone, compared with CO₂ concentration 380 μmol mol⁻¹ or low CO₂ concentration (100 μmol mol⁻¹). In addition, smaller cells of palisade tissue, more intercellular air spaces and partially two layers of palisade cells were observed in the leaves with root-zone CO₂ enrichment. Furthermore, there was a significant increase in the size of chloroplasts and starch grains, and the number of starch grains per chloroplast due to elevated CO₂ only under 21 % O₂. In addition, a significant decline in the thickness of grana and the number of lamellas, but no significant differences in the number of grana per chloroplast were observed under elevated CO₂ concentration. The accumulation of starch grains in the chloroplast under elevated CO₂ concentration could change the arrangement of grana thylakoids and consequently inhibited the absorption of sun radiation and photosynthesis of potato plants.     

不同杉木无性系叶片解剖结构对大气增温的响应

以10个不同杉木无性系幼林叶片为研究对象,在对研究地气候因子进行分析基础上,采用微波石蜡快速切片法制作叶片解剖结构,通过观测比较叶片8项解剖结构指标的差异规律,研究不同杉木无性系叶片解剖结构在大气增温背景下的响应特征。结果表明：（1）研究地2020年度夏季气温呈先上升后下降的趋势,降水量变化与之相反,呈现出明显的高温干旱现象。（2）不同杉木无性系叶片解剖结构相似,叶肉组织分化为栅栏组织和海绵组织,为典型异面叶;解剖结构指标除下表皮厚度外,其余指标在无性系间呈现出不同程度的改变,表现出对大气增温响应的形态可塑性差异。（3）相关性分析结果表明,叶片解剖结构指标之间存在一定自相关关系;气候因子中,月平均气温与栅栏组织呈极显著正相关关系（P<0.01）,月均降水量与栅栏组织、海绵组织、栅海比均呈极显著正相关关系（P<0.01）。（4）根据主成分分析结果,筛选出海绵组织、栅栏组织、叶片厚度、表皮组织和角质层为表征杉木叶片解剖结构的典型指标,反映出在大气增温背景下杉木叶片解剖结构变化特征以抵抗强烈光照和减少水分散发的特性。（5）不同杉木无性系叶片可塑性指数均值排序结果为W2 > P11 > S4 > P32 > P41 > P17 > P18 > S22 > S23 > 洋061,表明不同杉木无性系对大气增温的生态适应性与之相对应。综上可见,不同杉木无性系对环境的适应能力存在差异,叶片具有较强的形态可塑性变化,当气温改变时,杉木可通过叶片解剖结构调整以应对不断增加的大气温度带来的高温干旱复合逆境胁迫,未来应充分重视气候变化对杉木人工林生长发育的影响。     

Analysis of leaf tissue structures between rust-resistant and rust-susceptible Zoysia grass (<Emphasis Type="Italic">Zoysia japonica</Emphasis>)

The differences of leaf anatomic structures between rust-susceptible and -resistant zoysia (Zoysia japonica) were compared to reveal the physical defense mechanism of rust-resistant zoysia against Puccinia zoysiae. Zoysias were screened in greenhouse and fields of Beijing, Qingdao and Yangzhou from 2009 to 2012. After identified by inoculation test, rust-susceptible and rust-resistant zoysias were transplanted to flowerpots in Shangzhuang test field (Beijing, China) and were divided into indoor and outdoor groups. Leaves in the same position and formed in the same growth period of both rust-susceptible and rust-resistant zoysia strains were chosen for the current study. Wax content, the number and size of stomata, and the thickness of palisade tissue, spongy tissue, upper epidermis and lower epidermis were analyzed. All the anatomic structures of leaves stained with toluidine blue were observed under microscope. Leaf epidermis wax contents of rust-susceptible zoysia in indoor and outdoor groups were 30 and 39% lower than that of rust-resistant zoysia (p?<?0.05), respectively. The number of stomata in rust-resistant zoysia was 68% more than that in susceptible ones (p?<?0.05). Stomata of rust-resistant zoysia were 45% shorter and 65% narrower than those of rust-susceptible zoysia (p?<?0.05). The thickness of spongy parenchyma and palisade tissue in rust-resistant zoysia was significantly higher than that of the susceptible samples (p?<?0.05). Cell structure tightness and cell structure looseness of rust-resistant zoysia were tighter than that of rust-susceptible zoysia. Our study provides a histological understanding of the resistance mechanism of rust-resistant zoysia and might be useful for the identification of resistant varieties.