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2.
Plants have developed numerous strategies to cope with phosphorus (P) deficiency resulting from low availability in soils. Evolution of ethylene and up-regulation of root secreted acid phosphatase activity are common for plants in response to P deficiency. To determine the role of ethylene in response of plants to P deficiency, we investigated the effects of ethylene precursor (1-amino cyclopropane-1-carboxylic acid, ACC) and ethylene synthesis antagonists (aminoethoxyvinylglycine AVG, cobalt, Co 2+) on P concentrations in roots and shoots of Medicago falcata seedlings grown in P-sufficient (500 μM H 2PO 4−) and P-deficient (5 μM H 2PO 4−) solution. After transferring M. falcata seedlings from P-sufficient to P-deficient solution for 2 days, root P concentration was significantly reduced. The reduction in root P concentration was reversed by AVG and Co 2+, and a similar reduction in root P concentration of seedlings exposed to P-sufficient solution was observed by ACC. Expression of high-affinity phosphate transporters ( MfPT1, MfPT5) was enhanced by P-deficiency and this process was reversed by AVG and Co 2+. There was a marked increase in activity of root acid phosphatase (APase) and expression of gene encoding APase ( MfPAP1) under P-deficient conditions, and the increase in APAse activity and expression of MfPAP1 was inhibited by AVG and Co 2+. APase activity and expression of MfPAP1 expression in seedlings grown in P-sufficient solution were enhanced by ACC. Root and shoot P concentrations were increased when organic phosphorus was added to the P-deficient solution, and the increase in P concentration was significantly inhibited by AVG and Co 2+. These results indicate that ethylene plays an important role in modulation of P acquisition by possibly mobilizing organic P via up-regulating root APase activity and high-affinity phosphate transporters. 相似文献
3.
Alkaline phosphatase (APase) is one of the marine enzymes used by oceanic microbes to obtain inorganic phosphorus (P i) from dissolved organic phosphorus to overcome P-limitation. Marine APase is generally recognized to perform P-monoesterase activity. Here we integrated a biochemical characterization of a specific APase enzyme, examination of global ocean databases, and field measurements, to study the type and relevance of marine APase promiscuity. We performed an in silico mining of phoA homologs, followed by de novo synthesis and heterologous expression in E. coli of the full-length gene from Alteromonas mediterranea, resulting in a recombinant PhoA. A global analysis using the TARA Oceans, Malaspina and other metagenomic databases confirmed the predicted widespread distribution of the gene encoding the targeted PhoA in all oceanic basins throughout the water column. Kinetic assays with the purified PhoA enzyme revealed that this enzyme exhibits not only the predicted P-monoester activity, but also P-diesterase, P-triesterase and sulfatase activity as a result of a promiscuous behavior. Among all activities, P-monoester bond hydrolysis exhibited the highest catalytic activity of APase despite its lower affinity for phosphate monoesters. APase is highly efficient as a P-monoesterase at high substrate concentrations, whereas promiscuous activities of APase, like diesterase, triesterase, and sulfatase activities are more efficient at low substrate concentrations. Strong similarities were observed between the monoesterase:diesterase ratio of the purified PhoA protein in the laboratory and in natural seawater. Thus, our results reveal enzyme promiscuity of APase playing potentially an important role in the marine phosphorus cycle.Subject terms: Microbial biooceanography, Biogeochemistry, Microbial ecology 相似文献
4.
By generating and examining transgenic tomato overexpressing ath-miR399d grown in hydroponic conditions, in quartz sand, or in a polytunnel greenhouse vegetable soil culture, this study aimed to investigate the effects of miR399d from Arabidopsis on phosphorus (P) accumulation, P concentrations in transgenic tomato overexpressing ath-miR399d shoots, phosphate transporter expression, and proton secretion and acid phosphatase (APase) activity in roots. In the transgenic tomato, leaf P concentration increased significantly in an agricultural soil, and roots had higher uptake of P, as evidenced by leaf P concentrations and relative expression of the genes LePT1, LePT2, LePT4, and LePT5 in normal-P solution. Enhanced APase activity in transgenic roots and the outside medium led to superior hydrolysis of organic P, and increased proton extrusion by roots led to superior dissolution of AlPO 4. Thus, besides phosphate transporters, higher APase activity and strengthened acidification in the vicinity of the roots may be important mechanisms for transgenic tomato to scavenge or acquire P in soil. These results provide new understanding of miR399-overexpressing plants that accumulate excess P in shoots. 相似文献
5.
Phosphate starvation leads to a strong reduction in shoot growth and yield in crops. The reduced shoot growth is caused by extensive gene expression reprogramming triggered by phosphate deficiency, which is not itself a direct consequence of low levels of shoot phosphorus. However, how phosphate starvation inhibits shoot growth in rice is still unclear. In this study, we determined the role of OsCYCP4s in the regulation of shoot growth in response to phosphate starvation in rice. We demonstrate that the expression levels of OsCYCP4s, except OsCYCP4;3, were induced by phosphate starvation. Overexpression of the phosphate starvation induced OsCYCP4s could compete with the other cyclins for the binding with cyclin‐dependent kinases, therefore suppressing growth by reducing cell proliferation. The phosphate starvation induced growth inhibition in the loss‐of‐function mutants cycp4;1, cycp4;2, and cycp4;4 is partially compromised. Furthermore, the expression of some phosphate starvation inducible genes is negatively modulated by these cyclins, which indicates that these OsCYCP4s may also be involved in phosphate starvation signaling. We conclude that phosphate starvation induced OsCYCP4s might coordinate phosphate starvation signaling and cell cycle progression under phosphate starvation stress. 相似文献
8.
Muds from 12 types of soils were used as the only source of phosphorus in cultures of Scenedesmus dimorphus. Some muds supported as much algal growth as was obtained with 0.075 to 0.5 mg/1 of phosphorus, while little or no growth occurred in cultures which contained other muds as a source of phosphorus. Algal growth was correlated with the fractions of soil phosphorus which were extracted with the following solutions; I — the phosphorus — free nutrient solution, II — 0.05N HCl plus 0.025N H2SO4, III — 0.002N H2SO4 plus 3 g/l of K2SO4, and IV — 0.1N HCl plus 0.03N/NH4F. Additions of phosphorus to the soils prior to their use as muds increased the suitability of some as sources of phosphorus, but for others the added phosphorus was so tightly bound to the soil that little or none was available to S. dimorphus. The findings indicate that the type of soil in a pond will likely have a large influence on the efficiency of fertilization with phosphate fertilizers. 相似文献
9.
The acid phosphatase (orthophosphoric-monoester phosphohydrolase, EC 3.1.3.2) complement from dormant hazel ( Corylus avellana L.) seeds was found to exhibit significant electrophoretic heterogeneity partially attributable to the presence of distinct molecular forms. In axiferous tissue, total acid phosphatase activity increased in a biphasic fashion during chilling, a treatment necessary to alleviate seed dormancy. Three acid phosphatase isozymes were isolated from cotyledons of dormant hazel seeds by successive ammonium sulphate precipitation, size-exclusion, Concanavalin A affinity, cation- and anion-exchange chromatographies resulting in 75-, 389- and 191-fold purification (APase1, APase2, APase3, respectively). The three glycosylated isoforms were isolated to catalytic homogeneity as determined by electrophoretic, kinetic and heat-inactivation studies. The native acid phosphatase complement of hazel seeds had an apparent Mr of 81.5±3.5 kDa as estimated by size-exclusion chromatography, while the determined pI values were 5.1 (APase1), 6.9 (APase2) and 7.3 (APase3). The optimum pH for p-nitrophenyl phosphate hydrolysis was pH 3 (APase1), pH 5.6 (APase2) and pH 6 (APase3). The hazel isozymes hydrolysed a variety of phosphorylated substrates in a non-specific manner, exhibiting low Km and the highest specificity constant ( Vmax/ Km) for pyrophosphate. They were not primary phytases since they could not initiate phytic acid hydrolysis, while APase2 and APase3 had significant phospho-tyrosine phosphatase activity. Inorganic phosphate was a competitive inhibitor, while activity was significantly impaired in the presence of vanadate and fluoride.Abbreviations APase Acid phosphatase (EC 3.1.3.2) - ConA Concanavalin A–Sepharose 4B - CV Column volume - -GP -Glycerophosphate - IEF Isoelectric focusing - IP6 Phytic acid - pNPP p-Nitrophenyl phosphate - PAGE Polyacrylamide gel electrophoresis - PPi Pyrophosphate 相似文献
10.
Alkaline phosphatases (APases) play a crucial role in phosphorus (P) metabolism and regulation, but their physiological functions
largely remain unclear in cyanobacteria. Here, we identified four putative APase genes, designated as phoA- 709, phoD1- 709, phoD2- 709, and phoS- 709, in the cyanobacterium Anabaena sp. FACHB 709, and investigated their response to inorganic phosphate (P i) starvation. With the exception of phoD2- 709, three other APase genes were expressed at a constant and relative low level in P i-replete medium, whereas the expression of all four APase genes was elevated in response to P i starvation but phoA- 709 significantly. However, disruption of phoA- 709 did not affect the total APase activity but caused the expressional up-regulation of phoD1- 709 and phoS- 709 under P i-sufficient and P i-limiting conditions. These suggest that, the four APases of Anabaena sp. FACHB 709 are involved in P metabolism and regulation, and PhoA-709 is the main, yet dispensable, APase. 相似文献
11.
DDL1 encodes a mitochondrial phospholipase A 1 involved in acyl chain remodeling of mitochondrial phospholipids and degradation of cardiolipin in Saccharomyces cerevisiae. The deletion of DDL1 leads to respiratory growth defects. To elucidate the physiological role of DDL1, we screened for genes that, when overexpressed, suppress the respiratory growth defect of the DDL1 deletion mutant. Introduction of COQ8, COQ9, or COQ5, which are involved in coenzyme Q (CoQ) synthesis, using a multicopy vector suppressed the respiratory growth defect of the DDL1 deletion mutant. In contrast, introduction of COQ8 using a multicopy vector did not accelerate the growth of the deletion mutants of TAZ1 or CLD1, which encode an acyltransferase or phospholipase A 2, respectively, involved in the remodeling of cardiolipin. These results suggest genetic interactions between the mitochondrial phospholipase A 1 gene and the genes involved in CoQ synthesis. 相似文献
12.
The morphological and physiological responses of barley to moderate Pi deficiency and the ability of barley to grow on phytate
were investigated. Barley cultivars ( Hordeum vulgare L., Promyk, Skald and Stratus) were grown for 1–3 weeks on different nutrient media with contrasting phosphorus source: KH 2PO 4 (control), phytic acid (PA) and without phosphate (−P). The growth on −P medium strongly decreased Pi concentration in the
tissues; culture on PA medium generally had no effect on Pi level. Decreased content of Pi reduced shoot and root mass but
root elongation was not affected; Pi deficit had slightly greater impact on growth of barley cv. Promyk than other varieties.
Barley varieties cultured on PA medium showed similar growth to control. Extracellular acid phosphatase activities (APases)
in −P roots were similar to control, but in PA plants were lower. Histochemical visualization indicated for high APases activity
mainly in the vascular tissues of roots and in rhizodermis. Pi deficiency increased internal APase activities mainly in shoot
of barley cv. Stratus and roots of cv Promyk; growth on PA medium had no effect or decreased APase activity. Protein extracts
from roots and shoots were run on native discontinuous PAGE to determine which isoforms may be affected by Pi deficiency or
growth on PA medium; two of four isoforms in roots were strongly induced by conditions of Pi deficit, especially in barley
cv. Promyk. In conclusion, barley cultivars grew equally well both on medium with Pi and where the Pi was replaced with phytate
and only slightly differed in terms of acclimation to moderate deficiency of phosphate; they generally used similar pools
of acid phosphatases to acquire Pi from external or internal sources. 相似文献
13.
Summary Alkaline phosphatases (APase), both phosphomonoesterase (PMEase) and phosphodiesterase (PDEase) were studied in the cyanobacterium Anabaena oryzae for their specific requirements of temperature, pH, micro- and macronutrients and their activities in the presence of salinity and heavy metal stress. The alkaline phosphatases (PMEase and PDEase) are quite stable enzymes and require a narrow range of pH (pH 10–10.2) and temperature (35–40 °C) for their optimal activity.A pH of 10, 10.2 and 10.2 supported optimal activity of cellular PMEase, cellular PDEase and extracellular PMEase, respectively, whereas temperatures of 35, 38 and 40 °C were required for their optimal activity. The requirement for Ca 2+ and Mg 2+ as macronutrients and the significance of the micronutrients Zn 2+, Co 2+, Fe 2+, Mn 2+ and Cu 2+ in APase activity in the cyanobacterium suggests nutritional regulation of enzyme activity in A. oryzae. The metals Pb 2+, Cr 6+ and Ni 2+ severely inhibited APase activity, whereas the NaCl stress had a dual role, which was concentration dependent. NaCl stress at lower concentrations (≤20 mM) caused an increase in cellular PMEase activity while its higher concentration (>20 mM) favoured release of the extracellular PMEase . The decrease in cellular activity and an increase in extracellular activity suggest that the higher concentrations of salt stimulate the release of the enzyme.The data suggest that the cyanobacterium A. oryzae possess a potential application as biofertilizer in high salinity and alkaline (Ca 2+-rich) soils because of its ability to release PO 43− enzymatically under these conditions. 相似文献
14.
The gene PHO5 coding for one of the repressible acid phosphatases of the yeast Saccharomyces cerevisiae has been expressed at high efficiency in the baby hamster kidney (BHK) cell line. The expression vector was constructed from PHO5 driven by the human -actin promoter and was transfected into BHK cells by the calcium phosphate method. The recombinant APase (r-APase) which was secreted in active form from the cells was estimated by SDS/polyacrylamide gel electrophoresis to have molecular mass M
r=62000, indicating substitution of the polypeptide moiety by 2–3 asparagine-linked glycans. Analysis by sequential lectin affinity chromatography of glycopeptides obtained from r-APase with Pronase showed that the glycans are predominantly of the 2.2.4 triantennary and tetraantennary complex-type. These data suggest that the extensive glycosylation of yeast APase, which contains eight polymannose substituents, is not essential for secretion and expression of enzymatic activity of the transfected gene product.Abbreviations APase
acid phosphatase
- PBS
phosphate buffered saline
- TBS
Tris buffered saline
- con A
concanavalin A
- TCA
Tetracarpidium conophorum agglutinin 相似文献
15.
Phosphorus is a vital nutrient for cyanobacterial growth. Aside from dissolved inorganic phosphorus, dissolved organic phosphorus (DOP) is used by cyanobacterial species via the activity of alkaline phosphatase (APase), which likely plays an important role in acquiring phosphorus for algal growth in the same manner as it does in other bacteria. In this work, APase genes phoA, phoD, and phoX were found distributed in the cyanobacterial strains included in the algal genome collection of the NCBI database. PhoX has a wider distribution than the classical phoA and phoD. Furthermore, multiple types of APase genes were simultaneously identified in a single strain or genome. Anabaena flos-aquae FACHB-245 was selected as a typical strain to study the performance of cyanobacteria growing on DOP. In algal growth involving AMP or lecithin, APase regulates the release of phosphorus from DOP as confirmed by the relative quantification of phoD and phoX expression levels. Our results confirmed that the distribution of APase is prevalent in cyanobacteria and thus provides a new insight into the potential role of cyanobacterial APase on phosphorus acquisition in natural environment. 相似文献
16.
A novel P-deficient mutant of Arabidopsis thaliana, pho3, was isolated by screening for root acid phosphatase (APase) activity in plants grown under low-P conditions. pho3 had 30% less APase activity in roots than the wild type and, in contrast to wild-type plants, root APase activity did not
increase in response to growth in low P. However, shoot APase activity was higher in pho3 than in the wild-type plants. In addition, the pho3 mutant had a P-deficient phenotype, even when grown in P-sufficient conditions. The total P content of 11-d-old pho3 plants, grown in agar media with a plentiful supply of P, was about 25% lower than the wild-type level in the shoot, and
about 65% lower in the roots. In the rosette leaves of mature soil-grown pho3 plants the total P content was again reduced, to about 50% of wild-type levels. pho3 exhibited a number of characteristics normally associated with low-P stress, including severely reduced growth, increased
anthocyanin content (at least 100-fold greater than the wild type in soil-grown plants) and starch accumulation. The results
suggest that the mutant is unable to respond to low internal P levels, and may lack a transporter or a signalling component
involved in regulating P nutrition.
Received: 21 March 2000 / Accepted: 15 August 2000 相似文献
19.
Induction and secretion of acid phosphatases (APases) is thought to be an adaptive mechanism that helps plants survive and grow under phosphate (Pi) deprivation, in Arabidopsis, there are 29 purple acid phosphatase (AtPAP) genes. To systematically investigate the roles of different AtPAPs, we first identified knockout or knock-down T-DNA lines for all 29 AtPAP genes. Using these atpap mutants combined with in-gel and quantitative APase enzyme assays, we demonstrated that AtPAP12 and AtPAP26 are two major intracellular and secreted APases in Arabidopsis while AtPAPlo is mainly a secreted APase. On Pi-deficient (P-) medium or P- medium supplemented with the organophosphates ADP and fructose-6-phosphate (Fru-6-P), growth of atpaplo was significantly reduced whereas growth of atpap12 was only moderately reduced, and growth of atpap26 was nearly equal to that of the wild type (WT). Overexpression of the AtPAP12 or AtPAP26 gene, however, caused plants to grow better on P- or P- medium supplemented with ADP or Fru-6-P. Interest-ingly, Pi levels are essentially the same for the WT and overexpressing lines, although these two types of plants have significantly different growth phenotypes. These results suggest that the APases may have other roles besides enhancing internal Pi recycling or releasing Pi from external organophosphates for plant uptake. 相似文献
20.
To understand whether genotypic variation in acid phosphatase (APase) activity in rapeseed ( Brassica napus L.) induced by phosphorus (P) deficiency has impact on P efficiency, soil APase activity in the rhizosphere for rapeseed
P-efficient genotype 102 and P-inefficient genotype 105 was measured against organic and inorganic P sources in the pot experiment,
and the activities of root-secreted APase and leaf intracellular APase were investigated in different P-starvation periods
in the nutrient solution. Higher activity of root-secreted APase in B. napus was induced under low P conditions. However, P nutrition and P uptake efficiency of the plants supplied with organic P were
not directly related to the activity of root-secreted APase due to several confounding factors affecting APase availability.
The higher activity of leaf APase improved P remobilization in plants and played important roles in enhancing P use efficiency,
shown by the significant correlation between leaf APase activity and P use efficiency in a rapeseed recombinant inbred population
of 135 lines. 相似文献
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