共查询到20条相似文献,搜索用时 31 毫秒
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Ets factors regulate the polycystic kidney disease-1 promoter 总被引:3,自引:0,他引:3
Puri S Rodova M Islam MR Magenheimer BS Maser RL Calvet JP 《Biochemical and biophysical research communications》2006,342(4):1005-1013
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P Oettgen K Kas A Dube X Gu F Grall U Thamrongsak Y Akbarali E Finger J Boltax G Endress K Munger C Kunsch T A Libermann 《The Journal of biological chemistry》1999,274(41):29439-29452
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The Ets2 transcription factor inhibits apoptosis induced by colony-stimulating factor 1 deprivation of macrophages through a Bcl-xL-dependent mechanism 总被引:6,自引:0,他引:6
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Sevilla L Aperlo C Dulic V Chambard JC Boutonnet C Pasquier O Pognonec P Boulukos KE 《Molecular and cellular biology》1999,19(4):2624-2634
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GATA and Ets cis-acting sequences mediate megakaryocyte-specific expression. 总被引:15,自引:2,他引:15
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V Lemarchandel J Ghysdael V Mignotte C Rahuel P H Roméo 《Molecular and cellular biology》1993,13(1):668-676
The human glycoprotein IIB (GPIIB) gene is expressed only in megakaryocytes, and its promoter displays cell type specificity. We show that this specificity involved two cis-acting sequences. The first one, located at -55, contains a GATA binding site. Point mutations that abolish protein binding on this site decrease the activity of the GPIIB promoter but do not affect its tissue specificity. The second one, located at -40, contains an Ets consensus sequence, and we show that Ets-1 or Ets-2 protein can interact with this -40 GPIIB sequence. Point mutations that impair Ets binding decrease the activity of the GPIIB promoter to the same extent as do mutations that abolish GATA binding. A GPIIB 40-bp DNA fragment containing the GATA and Ets binding sites can confer activity to a heterologous promoter in megakaryocytic cells. This activity is independent of the GPIIB DNA fragment orientation, and mutations on each binding site result in decreased activity. Using cotransfection assays, we show that c-Ets-1 and human GATA1 can transactive the GPIIB promoter in HeLa cells and can act additively. Northern (RNA) blot analysis indicates that the ets-1 mRNA level is increased during megakaryocyte-induced differentiation of erythrocytic/megakaryocytic cell lines. Gel retardation assays show that the same GATA-Ets association is found in the human GPIIB enhancer and the rat platelet factor 4 promoter, the other two characterized regulatory regions of megakaryocyte-specific genes. These results indicate that GATA and Ets cis-acting sequences are an important determinant of megakaryocytic specific gene expression. 相似文献
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