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We describe here the isolation and characterization of OsiSAP8, a member of stress Associated protein (SAP) gene family from rice characterized by the presence of A20 and AN1 type Zinc
finger domains. OsiSAP8 is a multiple stress inducible gene, induced by various stresses, namely heat, cold, salt, desiccation, submergence, wounding,
heavy metals as well as stress hormone Abscisic acid. OsiSAP8 protein fused to GFP was localized towards the periphery of
the cells in the epidermal cells of infiltrated Nicotiana benthamiana leaves. Yeast two hybrid analysis revealed that A20 and AN1 type zinc-finger domains of OsiSAP8 interact with each other.
Overexpression of the gene in both transgenic tobacco and rice conferred tolerance to salt, drought and cold stress at seed
germination/seedling stage as reflected by percentage of germination and gain in fresh weight after stress recovery. Transgenic
rice plants were tolerant to salt and drought during anthesis stage without any yield penalty as compared to unstressed transgenic
plants.
OsiSAP8 is deposited in the Genbank with the Accession number AY345599. 相似文献
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Overexpression of ethylene response factor <Emphasis Type="Italic">TERF2</Emphasis> confers cold tolerance in rice seedlings 总被引:1,自引:0,他引:1
Rice (Oryza sativa L.) is a warm-season plant exposed to various stresses. Low temperature is an important factor limiting extension of rice
cultivation areas and productivity. Previously, we have demonstrated that tomato ERF protein TERF2 enhances freezing tolerance
of transgenic tobacco and tomato plants. Herein, we report that overexpression of TERF2 enhances transgenic rice tolerance to cold without affecting growth or agronomic traits. Physiological assays revealed that
TERF2 could not only increase accumulation of osmotic substances and chlorophyll, but also reduce reactive oxygen species
(ROS) and malondialdehyde (MDA) content and decrease electrolyte leakage in rice under cold stress. Further analysis of gene
expression showed that TERF2 could activate expression of cold-related genes, including OsMyb, OsICE1, OsCDPK7, OsSODB, OsFer1, OsTrx23, and OsLti6, in transgenic rice plants under natural condition or cold stress. Thus, our findings demonstrated that TERF2 modulated expression
of stress-related genes and a series of physiological adjustments under cold stress, indicating that TERF2 might have important
regulatory roles in response to abiotic stress in rice and possess potential utility in improving crop cold tolerance. 相似文献
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A. M. Gomaa G. N. Raldugina N. A. Burmistrova N. V. Radionov Vl. V. Kuznetsov 《Russian Journal of Plant Physiology》2012,59(1):105-114
Accumulation of soluble sugars (sucrose, fructose, and glucose), proline, phenols (total phenols and flavonoids), and antocyanins
during adaptation to low-temperature stress (4°C) of two lines of spring rape (Brassica napus L., cv. Westar) characterized by weak (Bn-1) and strong (Bn-3) expression of the Osmyb4 transgene was studied. Vegetatively propagated transgenic and wild-type plants were grown in the hydroponic culture at 24°C;
at the stage of 5–6 leaves, plants were exposed to 4°C for 5 days and then returned to the optimum temperature of 24°C for
recovery. Transgenic plants were established to manifest improved cold and frost tolerance, which was evident from more active
biomass accumulation at 4°C as compared with wild-type plants and from sustaining their viability after 2-day-long exposure
to −6°C. Determination of MDA content showed that one of the reasons of their improved cold tolerance was their capability
of maintaining oxidative homeostasis under low-temperature stress. This suggestion is supported by intense accumulation of
phenols and antocyanins, manifesting pronounced antioxidant effects, by transgenic plants during their cold adaptation. Thus,
during 2–5 days of plant exposure to 4°C, in transgenic plants the total content of phenols increased by 2.6–3.7 times, flavonoids
— by 3.7–4.7 times, and antocyanins — by 3.5–5.3 times as compared with control plants growing at 24°C. Transgenic Bn-3 plants with strong expression of the Osmyb4 gene accumulated phenols and antocyanins at 4°C more actively than Bn-1 plants characterized by weak expression of this gene. Transgenic rape plants subjected to cold stress accumulated more proline,
manifesting stress-protection effects, and lesser accumulation of soluble sugars. Before the beginning of experiment, the
content of soluble sugars was approximately similar in wild-type plants and transgenic lines; at 4°C their level in transgenic
plants was substantially lower than in control plants. As distinct from the process of cold adaptation, during recovery, the
content of all tested stress-protection compounds dropped sharply. The results obtained indicate that active expression of
the Osmyb4 gene from rice in the rape plants was accompanied not only by accumulation of compatible osmolytes but also by biosynthesis
of antioxidants of phenolic nature. 相似文献
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Supaporn Hasthanasombut Kanyaratt Supaibulwatana Masahiro Mii Ikuo Nakamura 《Plant Cell, Tissue and Organ Culture》2011,104(1):79-89
Betaine aldehyde dehydrogenase (BADH) is a major oxidative enzyme that converts betaine aldehyde to glycine betaine (GB),
an osmoprotectant compound in plants. Japonica rice (salt-sensitive) was genetically engineered to enhance salt tolerance
by introducing the OsBADH1 gene from Indica rice (salt-tolerant), which is a GB accumulator. We produced transgenic rice plants overexpressing the modified
OsBADH1 gene under the control of the maize ubiquitin promoter. The transgenic rice showed increased OsBADH1 gene expression and OsBADH1 enzyme production, resulting in the accumulation of GB. It also exhibited enhanced salt tolerance
in immature and mature transgenic rice seedlings. The adverse effect of salt stress on seed germination, the growth of immature
and mature seedlings, water status, and photosynthetic pigments was alleviated in transgenic seedlings. 相似文献
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Shiyang Li Xue-Ao Liu Lizi Zhao Huiqing Huang Bei Li Zhizhong Song Meixia Liang Hongxia Zhang Limin Wang Shenglin Zhou 《Phyton》2021,90(3):761-772
Seven in absentia (SINA) family proteins play a central role in plant growth, development and resistance to abiotic stress. However, their biological function in plant response to cold stress is still largely unknown. In this work, a seven in absentia gene IbSINA5 was isolated from sweet potato. Quantitative real-time polymerase chain reaction (qRT-PCR) analyses demonstrated that IbSINA5 was ubiquitously expressed in various tissues and organs of sweet potato, with a predominant expression in fibrous roots, and was remarkably induced by cold, drought and salt stresses. Subcellular localization assays revealed that IbSINA5-GFP fusion protein was mainly localized in cytoplasm and nucleus. Overexpression of IbSINA5 in sweet potato led to dramatically improved resistance to cold stress in transgenic plants, which was associated with the up-regulated expression of IbCOR (cold-regulated) genes, increased proline production, and decreased malondialdehyde (MDA) and H2O2 accumulation in the leaves of transgenic plants. Furthermore, transient expression of IbCBF3, a C-repeat binding factor (CBF) gene, in the leaf protoplasts of wild type sweet potato plants up-regulated the expression of both IbSINA5 and IbCOR genes. Our results suggest that IbSINA5 could function as a positive regulator in the cold signaling pathway through a CBF-SINA-COR mediated module in sweet potato, and have a great potential to be used as a candidate gene for the future breeding of new plant species with improved cold resistance. 相似文献
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S. M. Touhidul Islam R. S. Tammi Sneh L. Singla-Pareek Zeba Islam Seraj 《Acta Physiologiae Plantarum》2010,32(4):657-663
Rice yield is severely affected by high-salt concentration in the vicinity of the plant. In an effort to engineer rice for
improved salt tolerance Agrobacterium-mediated transformation of rice cv. Binnatoa was accomplished with the Pennisetum glaucum vacuolar Na+/H+ antiporter gene (PgNHX1) under the constitutive CaMV35S promoter. For the molecular analysis of putative transgenic plants, PCR and RT-PCR were performed.
Transgenic rice plants expressing PgNHX1 showed better physiological status and completed their life cycle by setting flowers and seeds in salt stress, while wild-type
plants exhibited rapid chlorosis and growth inhibition. Moreover, transgenic rice plants produced higher grain yields than
wild-type plants under salt stress. Assessment of the salinity tolerance of the transgenic plants at seedling and reproductive
stages demonstrated the potential of PgNHX1 for imparting enhanced salt tolerance capabilities and improved yield. 相似文献
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Hui Chen Rui An Jiang-Hua Tang Xiang-Huan Cui Fu-Shun Hao Jia Chen Xue-Chen Wang 《Molecular breeding : new strategies in plant improvement》2007,19(3):215-225
To develop a salt-tolerant upland rice cultivar (Oryza sativa L.), OsNHX1, a vacuolar-type Na+/H+ antiporter gene from rice was transferred into the genome of an upland rice cultivar (IRAT109), using an Agrobacterium-mediated method. Seven independent transgenic calli lines were identified by polymerase chain reaction (PCR) analysis. These
35S::OsNHX1 transgenic plants displayed a little accelerated growth during seedling stage but showed delayed flowering time and a slight
growth retardation phenotype during late vegetative stage, suggesting that the OsNHX1 has a novel function in plant development. Northern and western blot analyses showed that the expression levels of OsNHX1 mRNA and protein in the leaves of three independent transgenic plant lines were significantly higher than in the leaves of
wild type (WT) plants. T2 generation plants exhibited increased salt tolerance, showing delayed appearance and development of damage or death caused
by salt stress, as well as improved recovery upon removal from this condition. Several physiological traits, such as increased
Na+ content, and decreased osmotic potential in transgenic plants grown in high saline concentrations, further indicated that
the transgenic plants had enhanced salt tolerance. Our results suggest the potential use of these transgenic plants for further
agricultural applications in saline soil. 相似文献
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Supaporn Hasthanasombut Valentine Ntui Kanyaratt Supaibulwatana Masahiro Mii Ikuo Nakamura 《Plant biotechnology reports》2010,4(1):75-83
Glycine betaine has been reported as an osmoprotectant compound conferring tolerance to salinity and osmotic stresses in plants.
We previously found that the expression of betaine aldehyde dehydrogenase 1 gene (OsBADH1), encoding a key enzyme for glycine betaine biosynthesis pathway, showed close correlation with salt tolerance of rice. In
this study, the expression of the OsBADH1 gene in transgenic tobacco was investigated in response to salt stress using a transgenic approach. Transgenic tobacco plants
expressing the OsBADH1 gene were generated under the control of a promoter from the maize ubiquitin gene. Three homozygous lines of T2 progenies with single transgene insert were chosen for gene expression analysis. RT-PCR and western blot analysis results
indicated that the OsBADH1 gene was effectively expressed in transgenic tobacco leading to the accumulation of glycine betaine. Transgenic lines demonstrated
normal seed germination and morphology, and normal growth rates of seedlings under salt stress conditions. These results suggest
that the OsBADH1 gene could be an excellent candidate for producing plants with osmotic stress tolerance. 相似文献
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An osmotin from the resurrection plant Tripogon loliiformis (TlOsm) confers tolerance to multiple abiotic stresses in transgenic rice
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Osmotin is a key protein associated with abiotic and biotic stress response in plants. In this study, an osmotin from the resurrection plant Tripogon loliiformis (TlOsm) was characterized and functionally analyzed under abiotic stress conditions in T. loliiformis as well as in transgenic Nicotiana tabacum (tobacco) and Oryza sativa (rice) plants. Real‐time PCR analysis on mixed elicitor cDNA libraries from T. loliiformis showed that TlOsm was upregulated a 1000‐fold during the early stages of osmotic stresses (cold, drought, and salinity) in both shoots and roots but downregulated in shoots during heat stress. There was no change in TlOsm gene expression in roots of heat‐stressed plants and during plant development. The plasma membrane localization of TlOsm was showed in fluorescent‐tagged TlOsm tobacco plants using confocal laser scanning microscopic analysis. Transgenic rice plants expressing TlOsm were assessed for enhanced tolerance to salinity, drought and cold stresses. Constitutively expressed TlOsm in transgenic rice plants showed increased tolerance to cold, drought and salinity stress when compared with the wild‐type and vector control counterparts. This was evidenced by maintained growth, retained higher water content and membrane integrity, and improved survival rate of TlOsm‐expressing plants. The results thus indicate the involvement of TlOsm in plant response to multiple abiotic stresses, possibly through the signaling pathway, and highlight its potential applications for engineering crops with improved tolerance to cold, drought and salinity stress. 相似文献