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Sequence and analysis of the DNA encoding protective antigen of Bacillus anthracis 总被引:15,自引:0,他引:15
The nucleotide sequence of the protective antigen (PA) gene from Bacillus anthracis and the 5' and 3' flanking sequences were determined. PA is one of three proteins comprising anthrax toxin; and its nucleotide sequence is the first to be reported from B. anthracis. The open reading frame (ORF) is 2319 bp long, of which 2205 bp encode the 735 amino acids of the secreted protein. This region is preceded by 29 codons, which appear to encode a signal peptide having characteristics in common with those of other secreted proteins. A consensus TATAAT sequence was located at the putative -10 promoter site. A Shine-Dalgarno site similar to that found in genes of other Bacillus sp. was located 7 bp upstream from the ATG start codon. The codon usage for the PA gene reflected its high A + T (69%) base composition and differed from those of genes for bacterial proteins from most other sequences examined. The TAA translation stop codon was followed by an inverted repeat forming a potential termination signal. In addition, a 192-codon ORF of unknown significance, theoretically encoding a 21.6-kDa protein, preceded the 5' end of the PA gene. 相似文献
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A 330 bp region of the spinach nitrite reductase gene promoter directs nitrate-inducible tissue-specific expression in transgenic tobacco 总被引:4,自引:0,他引:4
Rajeev Rastogi Eduard Back Alois Schneiderbauer Caroline G. Bowsher Barbara Moffatt Steven J. Rothstein 《The Plant journal : for cell and molecular biology》1993,4(2):317-326
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Nadia Canu Roberta Possenti Anna Maria Rinaldi Eugenia Trani Andrea Levi 《Journal of neurochemistry》1997,68(4):1390-1399
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