Management of time by the cell and by the organism

Time-dependent regulations of cells and organisms can be analysed at different levels. One of these levels is the periodicity of cell functions such as cell division, metabolic processes (generation of ATP by glycolysis or oxidative mitochondrial processes) and the biosynthesis of cell constituents. Studies carried out on unicellular eukaryotes revealed the periodic, oscillatory nature of most of these processes. Time constants of these reactions vary from nanoseconds to hours-days, necessitating coupling mechanisms. Comparative studies revealed the coupling of the rapid processes (mitochondrial ATP generation) to the slower rhythms of the biosynthetic processes of macromolecules. Adenine nucleotides are involved in the coupling mechanisms between rapid and slow processes ("the slow dance of life to the music of time"). The mechanisms underlying these rhythmic processes involve either key allosteric regulatory enzymes (PFK for glycolysis) or "desensitization" of receptors by phosphorylation-dephosphorylation. At the organismic level the study of rhythmic processes is illustrated by the periodicity of heart beats, shown to exhibit multifractality, following apparently the formalism of deterministic chaos. Another example is the rhythmic oscillatory discharges of neuronal networks. The existence of subrhythmes mostly of epigenetic nature, facilitated probably the progressive adjustment of cells during evolution to the slow increase of day time since the separation of the moon from the earth. We analysed the mechanisms underlying the decline of these processes during aging. Loss of receptors or/and their uncoupling from their transmission pathway appear to be involved in most of these processes of decline. One conclusion of this review is the importance of epigenetic mechanisms both in the genesis and in the decline of these rythmic processes involved in time keeping by the cell.     

Grabbing the cat by the tail: manipulating molecules one by one

Methods for manipulating single molecules are yielding new information about both the forces that hold biomolecules together and the mechanics of molecular motors. We describe here the physical principles behind these methods, and discuss their capabilities and current limitations.     

主编寄语

<正>近30多年来,我国以令人瞩目的广度和深度走向现代化,伴随着社会经济的急速发展,人们的生活水平和行为模式发生了重大改变,一个老龄化社会的逼近迅速成为我们不可忽视的国情.这些重大的社会经济和生活变革已经导致慢性疾病成为我国人口健康当前和未来的重要威胁,其中老年性痴呆(Alzheimer's disease,AD)是一个重要组成部分.我国是世界上老年人口基数最大的国家,也是老年