Tropaeolaceae: 686. TROPAEOLUM SPECIOSUM

Tropaeolum speciosum Poepp. & Endl. is described and illustrated. Its habitat in the wild, and its pollination are discussed, and instructions for its successful cultivation are given.     

Tropaeolaceae: 685. TROPAEOLUM CILIATUM

Tropaeolum ciliatum Ruiz. & Pav. is described and illustrated. Its habitat in the wild, and its pollination, are discussed and instructions for its successful cultivation are given.     

Tropaeolaceae: 680. TROPAEOLUM BEUTHII

Tropaeolum beuthii Klotzsch is described and illustrated. Its history and distribution in the wild are discussed and notes on its cultivation are given.     

728. SALVIA OXYPHORA

The Bolivian endemic Salvia oxyphora Briq. is described and illustrated; the circumstances of its discovery, possible reasons for its reduced population in the wild and suggestions for its cultivation are given. The molecular status of Salvia and the probable break‐up of the genus as traditionally circumscribed are discussed.     

972. COELOGYNE CYMBIDIOIDES

Coelogyne cymbidioides Rchb.f., formerly known as Gynoglottis cymbidioides (Rchb.f.) J.J.Sm . from the mountains of Sumatra, is illustrated from a plant flowering at Kew. Its taxonomy, habitat in the wild and pollination are discussed. Details of its successful cultivation are given.     

871. ALLIUM AMPELOPRASUM var BABINGTONII

The bulbil‐bearing and proliferous wild leek, Allium ampeloprasum L. var. babingtonii (Borrer) Syme, from the south‐western coasts of England and Ireland is illustrated. Its history is described and suggestions for its cultivation are given.     

797. BULBOPHYLLUM AMBROSIA

The epiphytic orchid Bulbophyllum ambrosia bears solitary, sweet‐smelling, nectariferous flowers that attract honeybees in considerable numbers, but it is self‐incompatible, making fruit‐set in the wild infrequent. Even so, the species is relatively abundant throughout its range in Asia. The species is described and illustrated and its taxonomic history, ecology and cultivation are discussed.     

879. PASSIFLORA EMARGINATA

Passiflora emarginata Bonpl. (synonyms Astrophea emarginata (Bonpl.) M.Roem. and Passiflora caucaensis Holm‐Niels.), a medium‐sized tree from Colombia, is described and illustrated. Its history, taxonomy, conservation status, observations from the wild and cultivation including its propagation are discussed.     

Potential impact of genetically modified Lepidoptera‐resistant Brassica napus in biodiversity hotspots: Sicily as a theoretical model

The general increase of the cultivation and trade of Bt transgenic plants resistant to Lepidoptera pests raises concerns regarding the conservation of animal and plant biodiversity. Demand for biofuels has increased the cultivation and importation of oilseed rape (Brassica napus L.), including transgenic lines. In environmental risk assessments (ERAs) for its potential future cultivation as well as for food and feed uses, the impact on wild Brassicaeae relatives and on non‐target Lepidoptera should be assessed. Here we consider the potential exposure of butterflies as results of possible cultivation or naturalization of spilled seed in Sicily (Italy). Diurnal Lepidoptera, which are pollinators, can be exposed directly to the insecticidal proteins as larvae (mainly of Pieridae) through the host and through the pollen that can deposit on other host plants. Adults can be exposed via pollen and nectar. The flight periods of butterflies were recorded, and they were found to overlap for about 90% of the flowering period of B. napus for the majority of the species. In addition, B. napus has a high potential to hybridise with endemic taxa belonging to the B. oleracea group. This could lead to an exposure of non‐target Lepidoptera if introgression of the Bt gene into a wild population happens. A rank of the risk for butterflies and wild relatives of oilseed rape is given. We conclude that, in environmental risk assessments, attention should be paid to plant–insect interaction especially in a biodiversity hotspot such as Sicily.     

Tropaeolaceae: 679. TROPAEOLUM BRACHYCERAS

Tropaeolum brachyceras Hook. & Arnott is described and illustrated. An account of its history and distribution in the wild is given and notes on cultivation are provided. The hybrid T. ×tenuirostre, a cross between T. brachyceras and T. tricolor, is also discussed.     

Genetic instability of the short-living ascomycetous fungus <Emphasis Type="Italic">Podospora anserina</Emphasis> induced by prolonged submerged cultivation

Investigation of genetic variability of the short-living filamentous fungus Podospora anserina during its adaptation to conditions of prolonged submerged cultivation has been carried out for the first time. Cultivation of P. anserina under aeration (on a shaker) provides pronounced selective pressure, which makes it possible to obtain isolates with specific features, which are well adapted to cultivation in liquid media and have a life span several times exceeding that of the original strain. Static cultivation did not prevent the ageing of P. anserina. Repeated transfers in the shaker culture resulted in formation of mycelium deprived of the dark pigment melanin and actively producing carotenoids under illumination. The qualitative composition of P. anserina carotenoids was the same as in the closely-related species Neurospora crassa. The features obtained during the shaker cultivation (including changes in the colony morphology and decreased capacity for melanin synthesis) are inherited by their hybrids with the wild type strains, i.e., they resulted from the intragenomic rearrangements occurring during submerged cultivation of the fungus.     

Tropaeolaceae: 681. TROPAEOLUM TRICOLOR

Tropaeolum tricolor Sweet is described and illustrated. Its history and distribution in the wild are discussed and notes on cultivation are given.     

Lipid characterization of red alga Rhodymenia pseudopalmata (Rhodymeniales,Rhodophyta)

Rhodymenia pseudopalmata is a red alga that grows at the Caribbean coast of the Yucatan Peninsula and has been proven successful in cultivation. In this study we present the lipid composition of R. pseudopalmata collected from wild populations during three different seasons of 2013. Cultured material was also analyzed and compared in order to evaluate its value as feedstock for biotechnological uses. Thin layer chromatography, ¹ H and ¹³ C NMR spectroscopy and gas chromatography‐mass spectrometry were used to assess variations in their lipid composition. Our results showed that the dominant lipid classes were phospholipids both in wild and cultured materials. The phospholipids phosphatidylcholine and phosphatidylglycerol and the glycolipid monogalactosyldiacylglycerol were present in both wild and cultured R. pseudopalmata, whereas the phospholipid lysophosphatidylcholine was only found in wild material. Fatty acids (FAs) showed a high monounsaturated FAs (MUFAs) content with oleic acid (C18:1ω9) as the dominant compound (78 and 94% of the MUFAs for wild and culture materials, respectively). Saturated FAs (SFAs) represented approximately 90% of the total fatty acid content, with palmitic acid (C16:0) reaching approximately 83% of the SFA content. Rhodymenia pseudopalmata was low in polyunsaturated FAs when compared to other red algae. Other compounds such as 1‐heptadecene, 1‐hexadecene, 15‐heptadecenal, 3‐eicosene 6,10,14‐trimethyl‐2‐pentadecanone, phytol, and heptadecane were also found. Lipid composition differences between the wild and cultured algae suggest that light and nutrients can be manipulated to modify lipid composition. Based on its lipid composition and cultivation feasibility, R. pseudopalmata could be a potential source for nutraceuticals and biofuels production.     

Historical and botanical evidence of distribution, cultivation and utilization of Linum usitatissimum L. (flax) in China

Linum usitatissimum L. (flax) has been cultivated for more than 2,000 years in China. Throughout the history of its cultivation, flax was mainly used for oil and medicine. It was not until the last 100 years that fibre flax began to be cultivated and used. This paper discusses the distribution, cultivation and use of flax in China especially in the past, based on literature reviews and ethnobotanical surveys for its traditional uses. The modern distribution of wild flax species in China is also presented in this paper.     

Plant management among the Nahua and the Mixtec in the Balsas River Basin,Mexico: An ethnobotanical approach to the study of plant domestication

Different forms of management of wild, weedy, and domesticated plants carried out by the Nahua and the Mixtec in the Balsas River Basin, Mexico, are described. Along with cultivation of domesticated plants, these forms of plant management include gathering from wild populations; in situtolerance of plant individuals during clearings of natural vegetation; in situenhancement and protection of particular plants among populations of some species; as well as sowing or planting of propagules and transplantation of complete individuals of weedy and wild plants in controlled ex situenvironments. Processes of artificial selection and possible routes of domestication occurring in these forms of plant management are discussed.     

Bacterial Community Dynamics in the Marine Sponge <Emphasis Type="Italic">Rhopaloeides odorabile</Emphasis> Under In Situ and Ex Situ Cultivation

Cultivation of sponges is being explored to supply biomaterial for the pharmaceutical and cosmetics industries. This study assesses the impact of various cultivation methods on the microbial community within the sponge Rhopaloeides odorabile during: (1) in situ cultivation under natural environmental conditions, (2) ex situ cultivation in small flow-through aquaria and (3) ex situ cultivation in large mesocosm systems. Principal components analysis of denaturing gradient gel electrophoresis profiles indicated a stable microbial community in sponges cultured in situ (grown in the wild) and in sponges cultured ex situ in small flow-through aquaria over 12 weeks. In contrast, a shift in the microbial community was detected in sponges cultivated ex situ in large mesocosm aquaria for 12 months. This shift included (1) a loss of some stable microbial inhabitants, including members of the Poribacteria, Chloroflexi and Acidobacteria and (2) the addition of new microbes not detected in the wild sponges. Many of these acquired bacteria had highest similarity to known sponge-associated microbes, indicating that the sponge may be capable of actively selecting its microbial community. Alternatively, long-term ex situ cultivation may cause a shift in the dominant microbes that facilitates the growth of the more rare species. The microbial community composition varied between sponges cultivated in mesocosm aquaria with different nutrient concentrations and seawater chemistry, suggesting that these variables play a role in structuring the sponge-associated microbes. The high growth and symbiont stability in R. odorabile cultured in situ confirm that this is the preferred method of aquaculture for this species at this time.     

Tropaeolaceae: 683. TROPAEOLUM INCISUM

Tropaeolum incisum Sweet is described and illustrated. Its history and distribution in the wild are discussed and notes on cultivation are given. Reference is made to the vigorous form grown in Kew's Alpine House.     

1053. Furcraea macdougallii Matuda: Asparagaceae

Furcraea macdougallii Matuda, Falso Maguey Grande, is illustrated (Plate 1053). Its history in cultivation and distribution in Mexico are discussed. Its conservation status: probably extinct in the wild.     

Inferring ancient Agave cultivation practices from contemporary genetic patterns

Several Agave species have played an important ethnobotanical role since prehistory in Mesoamerica and semiarid areas to the north, including central Arizona. We examined genetic variation in relict Agave parryi populations northeast of the Mogollon Rim in Arizona, remnants from anthropogenic manipulation over 600 years ago. We used both allozymes and microsatellites to compare genetic variability and structure in anthropogenically manipulated populations with putative wild populations, to assess whether they were actively cultivated or the result of inadvertent manipulation, and to determine probable source locations for anthropogenic populations. Wild populations were more genetically diverse than anthropogenic populations, with greater expected heterozygosity, polymorphic loci, effective number of alleles and allelic richness. Anthropogenic populations exhibited many traits indicative of past active cultivation: fixed heterozygosity for several loci in all populations (nonexistent in wild populations); fewer multilocus genotypes, which differed by fewer alleles; and greater differentiation among populations than was characteristic of wild populations. Furthermore, manipulated populations date from a period when changes in the cultural context may have favoured active cultivation near dwellings. Patterns of genetic similarity among populations suggest a complex anthropogenic history. Anthropogenic populations were not simply derived from the closest wild A. parryi stock; instead they evidently came from more distant, often more diverse, wild populations, perhaps obtained through trade networks in existence at the time of cultivation.