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1.
2.
Thauera selenatis was grown anaerobically in minimal medium with either selenate or nitrate as the terminal electron acceptor and acetate as the carbon source and electron donor. The molar cell protein yields, YM-protein (selenate) and YM-protein (nitrate), were found to be 7.8 g cell protein/mol selenite formed and 7.5 g cell protein/mol nitrite formed, respectively. These values represent YM values of 57 and 55 g (dry weight)/mol acetate when selenate or nitrate was the electron acceptor, respectively. Based upon a calculated YATP value of 10.0 g (dry weight) cells/mol ATP, for growth on acetate in inorganic salts, growth with selenate as the terminal electron acceptor theoretically yielded 5.7 ATP/acetate oxidized, and 5.5 ATP when nitrate was the terminal electron acceptor. The results support the conclusion that energy is conserved via electron transport phosphorylation when selenate or nitrate reduction are the terminal electron acceptors during anaerobic growth with acetate. 相似文献
3.
The growth yields of 10 strains ofBacteroides fragilis isolated from a variety of clinical sites were determined in (a) basal medium, (b) basal medium plus heme, and (c) basal medium plus heme and menadione. The molar growth yield values, expressed as a function of glucose (YG) and ATP produced (YATP) for 24 h and 48 h were used for a comparison of different strains. Considerable variation occurred among strains, but in general only the results from 24-h grown cells were reproducible. After this period, the microscopic appearance of cells changed dramatically from well-formed, intact cells to large collections of extracellular vesicles and lysed cells. All strains were stimulated by heme, but marked differences occurred among strains. The addition of heme and menadione to the basal medium increased the YG values of some strains, whereas others were unaffected. Heme-cultured cells produced acetate, propionate, and succinate as major metabolic end products and possessed cytochrome b, menaquinone-10, and fumarate reductase activity. Strain NCTC 9343 grown without added heme by continuous culture or batch culture produced cells that were morphologically and biochemically similar. Under both conditions these cells lacked cytochromes, menaquinones, and fumarate reductase activity, but produced high levels of lactate and fumarate together with lower levels of acetate, propionate, and succinate. 相似文献
4.
The generation time ofP. mirabilis in defined and in complex medium is shorter in the presence of hydrogen acceptors than in their absence. In the presence
of hydrogen acceptors the molar growth yield for glucose and the acetate production are strongly increased. From the molar
growth yield and the acetate production YATP in defined medium was calculated as 5.5 g/mole, whereas in complex medium a value of 12.6 g/mole was obtained. The molar
growth yield, the acetate production, the amount of hydrogen acceptor reduced and YATP were used to calculate P/2e−ratios for phosphorylation coupled to electron transfer to oxygen, nitrate and tetrathionate as respectively 2.80; 1.48 and
1.23 in defined medium. Under anaerobic conditions in the presence of nitrate or tetrathionate as hydrogen acceptor in complex
medium a bend in the growth curve is observed. In the period of rapid growth the P/2e−ratio for nitrate reduction is of the same magnitude as that in defined medium, however much lower P/2e−ratios are found during the subsequent period of slow growth. The P/2e−ratios for tetrathionate reduction in complex medium for both growth periods are lower than those in defined medium. Most
probably these results indicate that during this period growth and energy production are uncoupled. Under aerobic conditions
in complex medium a constant YO value of 32.2 g/atom O is found during a short period of the growth curve. Afterwards when the cell density increases a steady
decrease of YO is observed. 相似文献
5.
P/2e ratios were calculated from anaerobic chemostat cultures of Paracoccus denitrificans with nitrogenous oxides as electron acceptor. P/2e ratios were calculated, using the Y
ATP
max
values determined for aerobic cultures. When succinate was the carbon and energy source the average P/2e values of the sulphate-and succinate-limited cultures with nitrate as electron acceptor were 0.5 and 0.7, respectively, and of the nitrite-limited culture 0.9. With gluconate as carbon and energy source the average P/2e values of the gluconate-limited with nitrate as electron acceptor and nitrate limited cultures were 0.9 and 1.1, respectively.H+/O ratios measured in cells obtained from sulphate-, succinate, nitrite-, gluconate-and nitratelimited cultures yielded respective average values of 3.4, 4.5, 3.5, 4.8 and 6.2 for endogenous substrates. From our data we conclude that sulphate-and nitritelimitation causes the loss of site I phosphorylation. Nitrite has no influence on the maximum growth yield on ATP. We propose that metabolism in heterotrophically grown cells of Paracoccus dentrificans is regulated on the level of phosphorylation in the site I region of the electron transport chain. 相似文献
6.
pH affected significantly the growth and the glucose fermentation pattern of Propionibacterium microaerophilum. In neutral conditions (pH 6.5–7.5), growth and glucose fermentation rate (qs) were optimum producing propionate, acetate,
CO2, and formate [which together represented 90% (wt/wt) of the end products], and lactate representing only 10% (wt/wt) of the
end products. In acidic conditions, propionate, acetate, and CO2 represented nearly 100% (wt/wt) of the fermentation end products, whereas in alkaline conditions, a shift of glucose catabolism
toward formate and lactate was observed, lactate representing 50% (wt/wt) of the fermentation end products. The energy cellular
yields (Y
X/ATP), calculated (i) by taking into account extra ATP synthesized through the reduction of fumarate into succinate, was 6.1–7.2
g mol−1. When this extra ATP was omitted, it was 11.9–13.1 g mol−1. The comparison of these values with those of Y
X/ATP in P. acidipropionici and other anaerobic bacteria suggested that P. microaerophilum could not synthesize ATP through the reduction of fumarate into succinate and therefore differed metabolically from P. acidipropionici.
Received: 8 April 2002 / Accepted: 8 May 2002 相似文献
7.
Summary The levels of several redox enzymes in a chlorate-resistant mutant of Proteus mirabilis, which is partially affected in the formation of formate hydrogenlyase, thiosulfate reductase and tetrathionate reductase, were compared with those of the wild type. The composition of the electron transport system of both strains was almost the same in cells grown aerobically, but very different in cells grown anaerobically. In the mutant, the cytochrome content increased twofold, whereas the level of the anaerobic enzymes is strongly diminished. The anaerobic formation of electron transport components in the mutant was, in contrast to that of the wild type, not influenced significantly by azide. During anaerobic growth with nitrate low levels of a functional nitrate reductase system were formed in the mutant. Under these conditions the formation of formate dehydrogenase, formate hydrogenlyase, formate oxidase, thiosulfate reductase, tetrathionate reductase, cytochrome b563,5 and partly that of cytochrome a2, was repressed. The repressive effect of nitrate, however, was completely abolished by azide. Therefore, it seems likely that a functional nitrate reductase system, rather than nitrate, controls the formation of the enzymes repressible by nitrate. 相似文献
8.
Anje Timmer-ten Hoor 《Antonie van Leeuwenhoek》1976,42(4):483-492
Yields of Thiobacillus denitrificans on different substrates were compared. The organism was grown in a chemostat at a dilution rate of 0.03 h-1. From the difference in the cell yields with (1) oxygen (6.40 g carbon per mole thiosulphate) and (2) nitrate (4.51 g carbon per mole thiosulphate) as an electron acceptor the experimental value for YATP was estimated to be 1.75. The efficiency of the biosynthetic system would be 42% if 1 ATP should be needed in reversed electron transport, and 57% if this was 2 ATP per electron pair.It could be calculated that during anaerobic oxidation of thiosulphate with nitrate 1.41 or 1.16 ATP per 2 electrons are generated if 1 or 2 ATP respectively per thiosulphate is formed in substrate-level phosphorylation. For aerobic oxidation these figures are 2.40 and 2.16, respectively 相似文献
9.
The rate of in-vivo nitrate reduction by leaf segments of Zea mays L. was found to decline during the second hour of dark anaerobic treatment. On transfer to oxygen the capacity to reduce nitrate under dark conditions was restored. These observations led to the proposal that nitrate reductase is a regulatory enzyme with ADP acting as a negative effector. The effect of ADP on the invitro activity of nitrate reductase and the changes in the in-vivo adenylate pool under dark-N2 and dark-O2 were investigated. It was found that ADP inhibited the activity of partially purified nitrate reductase. Similarly, the in-vivo anaerobic inhibition of nitrate reduction was associated with a build-up of ADP in the leaf tissue. Under anaerobic conditions nitrite accumulated and on transfer to oxygen the accumulated nitrite was reduced. To explain this phenomenon the following hypothesis was proposed and tested. Under anaerobic conditions the supply of reducing equivalents for nitrite reduction in the plastid becomes restricted and nitrite accumulates as a consequence. On transfer to oxygen this restriction is removed and nitrite disappears. This capacity to reduce accumulated nitrite was found to be dependent on the carbohydrate status of the leaf tissue. 相似文献
10.
Nitrate reductase and respiratory adaptation in Bacillus stearothermophilus 总被引:7,自引:6,他引:1
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R J Downey 《Journal of bacteriology》1966,91(2):634-641
Downey, R. J. (University of Notre Dame, Notre Dame, Ind.). Nitrate reductase and respiratory adaptation in Bacillus stearothermophilus. J. Bacteriol. 91:634-641. 1966.-Bacillus stearothermophilus 2184 required nitrate to grow in the absence of oxygen. Like many facultative microorganisms, the growth obtained anaerobically was considerably less than that obtained aerobically, even though the dissimilatory reduction of nitrate is, in effect, anaerobic respiration. The ability to reduce nitrate depended on the induction of nitrate reductase. Although oxygen at low levels did not retard induction of the enzyme, enzyme synthesis was considerably lessened by aeration. A semisynthetic medium containing nitrate supported aerobic growth of the thermophile but did not support anaerobic growth. The adaptation to nitrate resulted in a decrease in the level of cytochrome oxidase normally present in aerobically grown cells. Although the aerobic oxidation of succinate by the respiratory enzymes from aerobically grown cells was inhibited by 2-N-heptyl-4-hydroxyquinoline-N-oxide, the anaerobic oxidation of succinate by nitrate in a similar preparation from nitrate-adapted cells was not. The nitrate reductase in the bacillus was strongly inhibited by cyanide and azide but not by carbon monoxide. The nitrate reductase catalyzed the anaerobic oxidation of reduced nicotinamide adenine dinucleotide, and appeared to transfer electrons from cytochrome b(1) to nitrate. Cytochrome c(1) did not appear to be involved in the transfer. 相似文献
11.
Beggiatoa alba B18LD utilizes both nitrate and nitrite as sole nitrogen sources, although nitrite was toxic above 1 mM.B. alba coupledin vivo acetate oxidation, but not sulfide oxidation, with nitrate and nitrite reduction.B. alba could not, however, grow anaerobically with nitrate as the sole electron acceptor. Furthermore, the incorporation of acetate into macromolecules under anaerobic conditions with nitrate as the sole electron acceptor was less 10% of the incorporation with oxygen as the electron acceptor. The product of nitrate reduction byB. alba was ammonia; N2 or N2O were not produced. The nitrate reductase activity inB. alba was soluble and it utilized reduced flavins or methyl viologen and dithionite as electron donors. Pyrimidine nucleotides were not used as in vitro electron donors, either alone or with flavins in coupled assays. TheB. alba nitrate reductase activity was competitively inhibited with chlorate and was only mildly inhibited by azide and cyanide. Nitrate was not required for induction of theB. alba nitrate reductase, and neither oxygen nor ammonia repressed its activity. Thus,B. alba nitrate reductase appears to be an assimilatory nitrate reductase with unusual regulatory properties.Non-standard abbreviations MV
Methyl viologen
- DT
dithionite
- GS
glutamine synthetase
- GOGAT
glutamine 2-oxoglutarate aminotransferase
- PPO
2-diphenyloxazole
- POPOP
1,4-(bis)-[2-(5-phenyloxazolyl)] benzene
- TCA
trichloroacetic acid
- CCCP
carbonylcyanidem-chlorophenylhydrazone
- FCCP
carbonylcyanidep-trifluoromethoxyphenylhydrazone
- TTFA
thenoyltrifluoroacetone
- PHEN
1,10-phenanthroline
- HOQNO
2-heptyl 4-hydroxyquinoline-n-oxide
- 8HQ
8-hydroxyquinoline 相似文献
12.
Gotthard Schroff Ernst Zebe 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1980,138(1):35-41
Summary The anaerobic transformation of malate and succinate into propionate was demonstrated in homogenates and mitochondria isolated from the body wall musculature ofArenicola marina, a facultative anaerobic polychaete. Synthesis of propionate from succinate was enhanced by the addition of malate and ADP. In the presence of malate, acetate was formed in addition to propionate. Maximal quantities of both fatty acids were produced by mitochondria incubated with malate, succinate, and ADP. Since the rate of propionate production in this case was about the same as in homogenates when related to fresh weight, it is concluded that the enzymatic system involved is localized exclusively in the mitochondria. The rate of propionate production is correlated with the concentration of succinate, saturation being reached at about 5 mM. In tracer experiments using (methyl-14C)-malonyl-CoA, 2,3-14C-succinate, and 1-14C-propionate as precursors, the pathway of the transformation of succinate into propionate was examined. The results indicate that methylmalonyl-CoA is an intermediary product. It was shown that the synthesis of propionate from succinate is coupled to the formation of ATP. The ratio ATP/propionate was 0.76. Dinitrophenol had only a slight effect on this ratio, although the utilization of succinate was inhibited considerably. It is concluded that in vivo substrate level phosphorylation occurs equimolar to the formation of propionate from succinate.Abbreviations
Ap
5
A
P1,P5-di(adenosine-5-)pentaphosphate
-
DNP
2,4-dinitrophenol
-
mma
methylmalonic acid
-
mm-CoA
methylmalonyl-CoA
Enzymes EC 6.2.1.1
Acetate thiokinase (AMP)
- EC 3.6.1.3
actomyosin ATPase
- EC 2.7.4.3
adenylate kinase
- EC 2.8.3.1
CoA transferase
- EC 2.7.1.1
hexokinase
- EC 2.1.3.1
methylmalonyl-CoA carboxyltransferase
- EC 5.4.99.1
methylmalonyl-CoA isomerase
- EC 5.1.99.1
methylmalonyl-CoA racemase
- EC 6.4.1.3
propionyl-CoA carboxylase
- EC 1.2.4.1
pyruvate dehydrogenase
Supported by Deutsche Forschungsgemeinschaft Gr 456/6 相似文献
13.
J. G. J. Dekkers Hermine E. de Kok J. A. Roels 《Biotechnology and bioengineering》1981,23(5):1023-1035
Saccharomyces cerevisiae CBS 426 was grown aerobically and anaerobically in a glucose-limited chemostat. The flows of biomass, glucose, ethanol, carbon dioxide, oxygen, glycerol, and the elemental composition of the biomass were measured. Models for anaerobic and aerobic growth are constructed. Values for YATP and P/O are obtained from continuous culture data for aerobic growth; this YATP value is compared with that obtained from the anaerobic growth results. The ratio between the heat produced and the oxygen consumed increases if more glucose in fermented to ethanol and carbon dioxide. An equation for ?H/?O as a function of the respiratory quotient is given. 相似文献
14.
The expression of redox proteins of denitrification inThiosphaera pantotropha grown with oxygen,nitrate, and nitrous oxide as electron acceptors 总被引:2,自引:0,他引:2
The redox proteins and enzymes involved in denitrification inThiosphaera pantotropha exhibited a differential expression in response to oxygen. Pseudoazurin was completely repressed during batch or continuous
culture under oxic conditions. Cytochromecd
1 nitrite reductase was also heavily repressed after aerobic growth. Nitrite, nitric oxide, and nitrous oxide reductase activities
were detected in intact cells under some conditions of aerobic growth, indicating that aerobic denitrification might occur
in some circumstances. However, the rates of denitrification were much lower after aerobic growth than after anaerobic growth.
Growth with nitrous oxide as sole electron acceptor mimicked aerobic growth in some respects, implying that expression of
parts of the denitrification apparatus might be controlled by the redox state of a component of the electron transport chain
rather than by oxygen itself. Nevertheless, the regulation of expression of nitrous oxide reductase was linked to the oxygen
concentration. 相似文献
15.
P/O ratios were measured in membrane particles obtained from cells of Micrococcus denitrificans, while growing on different carbon sources. The membrane particles obtained from cells growing actively on glucose, succinate, ethanol and propanol as the carbon and energy sources catalyzed oxidative phosphorylation and yielded respective P/O ratios of 1.4, 1.2, 0.8, and 0.5 with NADH, and 0.8, 0.6, 0.6, and 0.5 with succinate as the electron donors. Not such a difference in P/O ratio is observed in intact resting cells grown with different carbon sources. It is concluded that the influence of the carbon source is probably directed towards the efficiency of oxidative phosphorylation in membrane particles and not in the growing cells.For the aerobic carbon source-limited chemostat cultures the following maximum growth yields were determined: 40.2 and 34.2 for succinate and oxgen, 41.7 and 36.5 for malate and oxygen, 81.4 and 39.4 for mannitol and oxygen, and 77.8 and 43.4 for gluconate and oxygen respectively. With a mathematical model (de K waadsteniet et al., in press) the P/O ratio was valued at 1.4–1.7. Y
ATP at =0.2 was valued at 8.7–10.9; Y
ATP
max
at 9.6–13.2 and m
e
at 0.6–4.5 for the most precise experiment (gluconate-limited). The calculation of these growth parameters has been discussed. 相似文献
16.
In a batch culture experiment the microaerophilic Campylobacter-like bacterium “Spirillum” 5175 derived its energy for growth from the reduction of nitrate to nitrite and nitrite to ammonia.
Hereby, formate served as electron donor, acetate as carbon source, and l-cysteine as sulfur source. Nitrite was quantitatively accumulated in the medium during the reduction of nitrate; reduction
of nitrite began only after nitrate was exhausted from the medium. The molar growth yield per mol formate consumed, Ym, was 2.4g/mol for the reduction of nitrate to nitrite and 2.0 g/mol for the conversion of nitrite to ammonia. The gain of
ATP per mol of oxidized formate was 20% higher for the reduction of nitrate to nitrite, compared to the reduction of nitrite
to ammonia. With succinate as carbon source and nitrite as electron acceptor, Ym was 3.2g/mol formate, i.e. 60% higher than with acetate as carbon source. No significant amount of nitrous oxide or dinitrogen
was produced during growth with nitrate or nitrite both in the presence or absence of acetylene. No growth on nitrous oxide
was found. The hexaheme c nitrite reductase of “Spirillum” 5175 was an inducible enzyme. It was present in cells cultivated with nitrate or nitrite
as electron acceptor. It was absent in cells grown with fumarate, but appeared in high concentration in “Spirillum” 5175 grown
on elemental sulfur. Furthermore, the dissimilatory enzymes nitrate reductase and hexaheme c nitrite reductase were localized in the periplasmic part of the cytoplasmic membrane. 相似文献
17.
Whole‐cell biocatalysis for C–H oxyfunctionalization depends on and is often limited by O2 mass transfer. In contrast to oxygenases, molybdenum hydroxylases use water instead of O2 as an oxygen donor and thus have the potential to relieve O2 mass transfer limitations. Molybdenum hydroxylases may even allow anaerobic oxyfunctionalization when coupled to anaerobic respiration. To evaluate this option, the coupling of quinoline hydroxylation to denitrification is tested under anaerobic conditions employing Pseudomonas putida (P. putida) 86, capable of aerobic growth on quinoline. P. putida 86 reduces both nitrate and nitrite, but at low rates, which does not enable significant growth and quinoline hydroxylation. Introduction of the nitrate reductase from Pseudomonas aeruginosa enables considerable specific quinoline hydroxylation activity (6.9 U gCDW?1) under anaerobic conditions with nitrate as an electron acceptor and 2‐hydroxyquinoline as the sole product (further metabolization depends on O2). Hydroxylation‐derived electrons are efficiently directed to nitrate, accounting for 38% of the respiratory activity. This study shows that molybdenum hydroxylase‐based whole‐cell biocatalysts enable completely anaerobic carbon oxyfunctionalization when coupled to alternative respiration schemes such as nitrate respiration. 相似文献
18.
Effi Eisenmann Joachim Beuerle Klaus Sulger Peter M. H. Kroneck Wolfram Schumacher 《Archives of microbiology》1995,164(3):180-185
Sulfurospirillum deleyianum grew in batch culture under anoxic conditions with sulfide (up to 5 mM) as electron donor, nitrate as electron acceptor,
and acetate as carbon source. Nitrate was reduced to ammonia via nitrite, a quantitatively liberated intermediate. Four moles
of sulfide were oxidized to elemental sulfur per mole nitrate converted to ammonia. The molar growth yield per mole sulfide
consumed, Ym, was 1.5 ± 0.2 g mol–1 for the reduction of nitrate to ammonia. By this type of metabolism, S. deleyianum connected the biogeochemical cycles of sulfur and nitrogen. The sulfur reductase activity in S. deleyianum was inducible, as the activity depended on the presence of sulfide or elemental sulfur during cultivation with nitrate or
fumarate as electron acceptor. Hydrogenase activity was always high, indicating that the enzyme is constitutively expressed.
The ammonia-forming nitrite reductase was an inducible enzyme, expressed when cells were cultivated with nitrate, nitrite,
or elemental sulfur, but repressed after cultivation with fumarate.
Received: 13 March 1995 / Accepted: 29 May 1995 相似文献
19.
The anaerobic rumen bacterium Wolinella succinogenes was able to grow by respiration with dimethylsulphoxide (DMSO) as electron acceptor and formate or H2 as electron donors. The growth yield amounted to 6.7 g and 6.4 g dry cells/mol DMSO with formate or H2 as the donors, respectively. This suggested an ATP yield of about 0.7 mol ATP/mol DMSO. Cell homogenates and the membrane fraction contained DMSO reductase activity with a high K
m (43 mM) for DMSO. The electron transport from H2 to DMSO in the membranes was inhibited by 2-(heptyl)-4-hydroxyquinoline N-oxide, indicating the participation of menaquinone. Formation of DMSO reductase activity occurred only during growth on DMSO, presence of other electron acceptors (fumarate, nitrate, nitrite, N2O, and sulphur) repressed the DMSO reductase activity. DMSO can therefore be used by W. succinogenes as an acceptor for phosphorylative electron transport, but other electron acceptors are used preferentially.Abbreviations
DMN
2,3-Dimethyl-1,4-naphthoquinone
-
DMNH
2
Reduced DMN
-
DMS
Dimethylsulphide (CH3)2S
-
DMSO
Dimethylsulphoxide (CH3)2SO
-
HQNO
2-(Heptyl)-4-hydroxyquinoline-N-oxide
-
TMAO
Trimethylamine-N-oxide
-
Y
s
Growth yield for substrate S 相似文献
20.
ATP formation associated with fumarate and nitrate reduction in growing cultures of Veillonella alcalescens 总被引:1,自引:0,他引:1
Wytske de Vries Theresia R. M. Rietveld-Struijk A. H. Stouthamer 《Antonie van Leeuwenhoek》1977,43(2):153-167
Molar growth yields, fermentation balances and enzyme activities were measured in Veillonella alcalescens grown anaerobically with different substrates in the absence or presence of fumarate or nitrate. The molar growth yields on malate (14.3 g dry wt bacteria/mole substrate) and citrate (19.3) were higher than that on lactate (8.6). The molar growth yield on lactate was increased to 15.5 or 19.8 by the addition of fumarate or nitrate, respectively, to the growth medium, and the molar growth yield on citrate was increased to 25.3 by addition of nitrate. Active growth yield was 25.5. From fermentation balances and fermentation systems similar YATP values (g dry wt bacteria/mole ATP) were calculated for all substrates or mixtures of substrates assuming that one mole of ATP is generated at the electron transport from pyruvate, NADH and NADPH to nitrate or fumarate whereas ATP is not produced in the electron transport from lactate to fumarate or nitrate, and, therefore, this assumption was considered to reflect the actual situation. The mean YATP value at a doubling time of 1 h was 16.5 g dry wt bacteria/mole ATP for growth without an added hydrogen acceptor, 14.4 for growth with fumarate, and 14.2 for growth with nitrate. 相似文献