Hyperbaric oxygen therapy ameliorates osteonecrosis in patients by modulating inflammation and oxidative stress

Early stages of avascular necrosis of the femoral head (AVNFH) can be conservatively treated with hyperbaric oxygen therapy (HBOT). This study investigated how HBOT modulates inflammatory markers and reactive oxygen species (ROS) in patients with AVNFH. Twenty-three male patients were treated with two cycles of HBOT, 30 sessions each with a 30 days break between cycles. Each session consisted of 90?minutes of 100% inspired oxygen at 2.5 absolute atmospheres of pressure. Plasma levels of tumor necrosis factor alfa (TNF-α), interleukin 6 (IL-6), interleukin 1 beta (IL-1β) and ROS production were measured before treatment (T0), after 15 and 30 HBOT sessions (T1 and T2), after the 30-day break (T3), and after 60 sessions (T4). Results showed a significant reduction in TNF-α and IL-6 plasma levels over time. This decrease in inflammatory markers mirrored observed reductions in bone marrow edema and reductions in patient self-reported pain.     

Influence of hyperbaric oxygen therapy on bone metabolism in patients with neoplasm

BackgroundHyperbaric oxygen therapy (HBOT) is useful in the treatment of complications due to radiotherapy in patients with neoplasm. Its effects on bone metabolism are unclear. In our study, we analyzed the effects of HBOT on bone remodeling in oncological patients with radiotherapy.Materials and methodsProspective clinical study in 23 patients with neoplasms undergoing treatment with HBOT due to complications of radiotherapy (hemorrhagic cystitis, proctitis or radionecrosis) and 25 patients with chronic anal fissure. The average number of HBOT sessions was 20 ± 5 (100% oxygen, 2.3 atmospheres and 90 min per day). Serum levels of aminoterminal propeptide of type I collagen (P1NP), C terminal telopeptide of type I collagen (CTX), alkaline phosphatase (AP), 25hydroxyvitamin D (25-OHD), parathyroid hormone (PTH), were measured at 3 time points: T0 (before beginning HBOT), T1 (at the end of HBOT) and T2 (6 months after HBOT).ResultsAt baseline, the patients with neoplasm have higher bone turnover than those with anal fissure. These differences were 41% in CTX (0.238 ± 0.202 ng/mL in neoplasm and 0.141 ± 0.116 ng/mL in fissure; p = 0.04), 30% for PTH (46 ± 36 pg/mL in neoplasm and 32 ± 17 pg/mL in fissure; p = 0.04) and 15% for alkaline phosphatase (80 ± 24 U/L in neoplasm and 68 ± 16 U/L in fissure; p = 0.04). In the group with neoplasm, the values of P1NP decreased 6% after HBOT (T0: 49 ± 31 ng/mL, T2: 46 ± 12 ng/mL; p = 0.03). Also, there were non-significant decreases in PTH (−34%) and CTX (−30%).ConclusionsPatients with neoplasm and complications with radiotherapy have an increase in bone remodeling that may be diminished after HBOT.     

Serum lipid levels in thyroid dysfunction with special reference to transient elevation during treatment in hyperthyroid Graves' disease

Lipid metabolism was examined in patients with hyper- or hypothyroidism. Compared with corresponding age and sex matched controls, serum total cholesterol (T-chol), low density lipoprotein cholesterol (LDL-chol), phospholipid (PL) and LDL levels were significantly low and free fatty acid (FFA) levels were high with apparently normal triglyceride (TG), very low density lipoprotein (VLDL) and high density lipoprotein cholesterol (HDL-chol) levels in 61 hyperthyroid patients, while T-chol, LDL-chol, TG, PL, VLDL and LDL levels were high with normal FFA and HDL-chol levels in 31 hypothyroid patients. Serum lipid levels were then repeatedly measured in 7 men and 7 women with hyperthyroid Graves' disease before treatment (stage I), just after the patients became euthyroid with anti-thyroid drug (stage II) and more than 2 months after the patients remained euthyroid (stage III). Serum T-chol, LDL-chol, PL and LDL levels were low at stage I, significantly elevated at stage II and then normalized at stage III. Transient but significant elevation of serum TG, VLDL and HDL-chol levels at stage II were also observed in men. Accelerated catabolism and anabolism of lipid has been reported in hyperthyroidism. Transient elevation of serum lipid levels suggests a more rapid improvement in catabolism than in anabolism of lipid in an early stage of the medical treatment for hyperthyroidism.     

TSH and prolactin secretions in Hashimoto's thyroiditis following withdrawal of thyroid hormone therapy

Changes in the pituitary-thyroid axis in patients with Hashimoto's thyroiditis following withdrawal of thyroid suppressive therapy were analyzed. The group of patients with thyroid adenoma served as control (group I). Patients with Hashimoto's thyroiditis were divided into 2 groups on the basis of serum TSH levels 8 weeks after discontinuing the exogenous thyroid hormone (group II, less than 10 microunits/ml; group III, more than 10 microunits/ml). During treatment with L-T4(200 micrograms/day) or L-T3(50 micrograms/day), there was no significant difference in serum T4-I and T3 levels among the three groups. Following L-T4 withdrawal, basal serum TSH levels were higher at 2 to 8 weeks in groups II and III than in group I. Serum TSH response to TRH was greater at 4 to 8 weeks in groups II and III than in group I. Following L-T3 withdrawal, basal serum TSH levels were higher at 1 and 2 weeks in group II than in group I, while those of group III were consistently higher during the study. Higher TSH responses to TRH were observed at 1 to 8 weeks in groups II and III. Neither basal nor TRH-induced prolactin (PRL) secretion differed significantly among the three groups. We have demonstrated that pituitary TSH secretion in patients with Hashimoto's thyroiditis is affected more by withdrawal of thyroid hormone therapy than in patients with thyroid adenoma. In addition, the present findings suggest a difference between the sensitivity of thyrotrophs and lactotrophs in Hashimoto's thyroiditis after prolonged thyroid therapy is discontinued.     

Blood and urine iodine levels in patients with gastric cancer

In this study, we aimed to investigate whether there is any relationship between gastric cancer and iodine concentrations in blood and urine in the northeast Anatolia region, where iodine deficiency is common. A total of 56 patients, diagnosed as gastric cancer and 25 healthy volunteers were included in the study. The methods used were based on the Sandell-Kolthoff reaction. The urine iodine concentration (UIC) and serum protein-bound iodine (PBI) levels were higher in patients with gastric cancer compared with healthy control subjects. The UIC in stage IV was higher than all other stages and the control group. The UIC was higher in stages III and IV compared with stages I and II. However, serum PBI levels in stage III were higher compared with stages I, and II and also control group. The serum PBI level in stage IV was higher than stage II and the control group. In the patient and control groups, there were no significant differences in serum PBI and UIC with regard to age or sex. Our results suggested that urinary and blood iodine concentration might be a useful marker for following the disease.     

Early and late effects of hyperbaric oxygen treatment on oxidative stress parameters in diabetic patients

Exposure to hyperbaric oxygen leads to increased amount of reactive oxygen species (ROS) that are derived from various sources. After the discovery that ROS can function as signaling molecules, the idea of ROS being hazardous to biological tissues has been challenged. The aim of this study was to examine the changes in oxidative stress parameters in diabetics undergoing hyperbaric oxygen therapy (HBOT) due to foot ulcers. Twenty patients, who received HBOT for diabetic foot ulcers, were included in the study. Blood samples were taken before HBOT and 30 min after exit from the chamber, on the day of the first and the fifteenth HBOT sessions. They were used for the determinations of malondialdehyde (MDA), 8-isoprostane and advanced oxidation protein products (AOPPs). 8-Isoprostane and AOPP levels were not altered significantly after the first HBOT session, while both were increased on the fifteenth day (p<0.05). MDA was significantly increased only after the first HBOT session, and remained unchanged on the fifteenth day (within-day variations). Plasma AOPP levels were lowered significantly after fifteen consecutive HBOT sessions (between-day variations). Decreased AOPP levels suggest that increased oxygenation of tissues due to HBO therapy may activate some endogenous factors that prevent hazardous effects of the disease itself.     

Relationship between Bone Mineral Density and Serum Osteoprotegerin in Patients with Chronic Heart Failure

Purpose

Heart failure (HF) had been reported with increased risk of hip fractures. However, the relationship between circulating biomarkers and bone mineral density (BMD) in chronic HF remained unclear.

Methods

This is a cross-sectional study which recruited stable chronic HF from registry of the Heart Failure Center of National Taiwan University Hospital. Patients underwent dual-energy x-ray absorptiometry (DEXA) measurements at hip and lumbar spines and biochemical assessments including B-type natriuretic peptide (BNP-32), myostatin, follistatin and osteoprotegerin (OPG).

Results

A total of 115 stable chronic HF individuals with left ventricular ejection fraction (EF) <45% (74% of male, mean age at 59) were recruited with 24 patients in NYHA class I, 73 patients in NYHA class II and 18 patients in NYHA class III. Results of BMD showed that Z scores of hip in NYHA III group (−0.12±1.15) was significantly lower than who were NYHA II (0.58±1.04). Serum OPG was significantly higher in subjects of NYHA III (9.3±4.6 pmol/l) than NYHA II (7.4±2.8 pmol/l) or NYHA I (6.8±3.6 pmol/l) groups. There’s a significant negative association between log transformed serum OPG and trochanteric BMD (R = −0.299, P = 0.001), which remained significant after multivariate analysis.

Conclusions

Our study demonstrated an inverse association between serum OPG and trochanteric BMD in patients with HF. OPG may be a predictor of BMD and an alternative to DEXA for identifying at risk HF patients for osteoporosis.     

Correlation between serum enzymes and serum unsaturated vitamin B12 binding proteins in primary liver carcinoma

The serum unsaturated vitamin B12-binding capacity (UBBC), unsaturated transcobalamin (UTC) I, UTC II, UTC III levels, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase activities and bilirubin concentration were estimated in 61 patients with liver diseases (31 with hepatoma, 30 with viral hepatitis). The levels of serum cobalamin, UTC I, UTC III, UBBC, alanine and aspartate aminotransferases, and bilirubin were raised in both hepatoma and viral hepatitis patients. Serum UTC II was reduced in both conditions. Alkaline phosphatase activity was significantly increased in hepatoma. Four significant correlations were observed among these parameters in the hepatoma patients while only one significant correlation was observed in viral hepatitis.     

The relationship between trace elements and cardiac markers in acute coronary syndromes

Previous studies have demonstrated increased serum copper and iron levels and decreased selenium and zinc levels in patients with myocardial infarction. Furthermore, the prognostic value of the levels of trace elements in myocardial infarction has been stressed. We examined serum levels of Cu, Fe, Zn and Se, as well as glutathione peroxidase (GPx), a selenoenzyme with antioxidant properties, and C-reactive protein (CRP), a marker of inflammation, in acute coronary syndromes (ACS) regarding their relationship to cardiac troponins and creatine kinase-MB mass (CK-MBm), important prognostic markers. Serum trace elements, GPx activity and CRP were determined in 70 patients with ACS who were admitted within 12 h after the onset. Differences in these parameters were evaluated in three groups of patients divided according to the levels of cardiac markers: group III consisted of patients with high increases in cTnT, cTnI and CK-MBm (> or =0.9 ng/mL, > or =1.0 ng/mL, > or =30 ng/mL, respectively), patients with milder increases in these markers were included in groups II and I consisted of patients with values just above the upper reference limits. Serum Fe levels increased significantly in group II and even more prominently in group III compared to group I (p = 0.04, 0.002, respectively). There was no significant difference between groups II and III. The increase in serum Cu was significant in group III compared to both groups II and I (p = 0.04, 0.001, respectively). There was no significant difference between groups I and II regarding Cu and Zn. The decrease in serum Se and GPx levels was significant only between groups III and I (p = 0.004 for Se and p = 0.0001 for GPx). CRP levels showed a significant increase in group III compared to groups II and I (p = 0.03 and 0.001). CRP showed a significant positive and GPx a significant negative correlation to the cardiac markers cTnT, cTnI and CK-MBm. Cu was positively correlated to all cardiac markers, while the positive correlation between Fe and cardiac markers was significant only for cTnI. Both Zn and Se were negatively correlated to cTnT, and Se was also to cTnI. In conclusion, the increase in serum levels of Cu and Fe and the decrease in serum levels of Zn and Se in patients with higher levels of troponins and CK-MBm imply that trace element levels are related to the degree of myocardial damage and thus may play a role in the pathogenesis of ischemic heart disease. The strong correlations between cardiac markers and both CRP and GPx suggest that these parameters are promising prognostic factors in acute coronary syndromes.     

Growth hormone secretion during sleep. II. Interrelationships between growth hormone secretion, insulin-like growth factor I and sex steroids

The serum levels of insulin-like growth factor I (IGF I), dehydroepiandrosterone sulfate (DHAS), testosterone (T) and estradiol (E2) have been measured in 78 prepubertal and 57 early pubertal patients referred for short stature, at the same time when their secretion of GH was evaluated both during nocturnal sleep and by two conventional stimulation tests. According to the results of GH measurements they were considered as having a normal secretion of GH (group I), a complete GH deficiency (group II), a partial GH deficiency (group III), low responses to stimuli with normal secretion during sleep (group IV) or a nocturnal neurosecretory dysfunction (group V). Though widely scattered, the IGF I levels showed the following characteristics: a significant increase at puberty from 0.77 to 1.29 U/ml (p less than 0.001) in the so-called endocrinologically normal patients of group I, not in the other groups; in the prepubertal patients of group I, a correlation of IGF I with chronological age (r = 0.47, p less than 0.005) and bone age (r = 0.52, p less than 0.002); significantly reduced IGF I levels in patients of group II having complete GH deficiency (p less than 0.001); no significant differences between prepubertal patients with partial or atypical GH deficiency from groups III, IV, V and prepubertal patients from group I; lower pubertal levels in groups III, IV, V than in pubertal patients from group I (p less than 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)     

Low T3 syndrome in cancer patients in relation to weight loss, intravenous hyperalimentation therapy and age

In order to investigate the relation of weight loss and intravenous hyperalimentation therapy to low T3 syndrome, serum T3, T4. rT3 and TBG were determined by radioimmunoassay in 105 cancer patients. The cancer patients were classified into 3 groups, Group I, II and III depending on the grade of weight loss, ranging up to a 5% change in weight loss from a healthy condition, from 5 to 9%, and more than 10%, respectively. Cancer patients under age 59 showed no significant difference in serum T3, T4, rT3 and TBG among these 3 groups. However serum T3 and T3/T4 in cancer patients at age 60 and over were significantly reduced in group III, compared to groups I and II. Serum rT3 values were significantly elevated in group III of elderly cancer patients. The incidence of low T3 syndrome in group III of elderly cancer patients was also significantly higher than in groups I and II. In three out of 5 cancer patients with low T3 syndrome, serum T3 values increased after the intravenous hyperalimentation therapy, whereas no significant change in serum T3 values was observed in two patients who died within one day after the final examination. It is concluded that weight loss produced different effects on peripheral conversion of T4 to T3 between cancer patients under age 59 and over age 60 and glucose plays an important role in the pathogenesis of low T3 syndrome except cases with very poor prognosis.     

Daytime changes in oocyte development with relation to the tide for the Hawaiian saddleback wrasse, Thalassoma duperrey

Hourly variations in oocyte stages were characterized by size class and histological examination inassociation to the daytime tidal cycle for the Hawaiian saddleback wrasse, Thalassoma duperrey . Stage I (previtellogenic), stage II (vitellogenic) and stage III (hydrated) oocytes were identified as distinct clutches. During the autumn the profiles of oocytes stages showed rapid, group-synchronous development from stage I to stage III. Concurrent increases in percentages of both stage III oocytes and the gonadosomatic index (GSI) were positively correlated to and occurred I h before the high tide. Increases in stage I and stage II oocytes 1 h after high tide indicated development of new clutches following a decline in stage III oocytes, and the appearance of post-ovulatory follicles. The profile of stage II oocytes always exceeded 30% of the ovary. Lower GSI and percentages of stage III oocytes reflect significantly reduced reproduction in the summer: as in the autumn, both factors were significantly correlated, but neither variable showed a statistical relationship to the tide. Nevertheless, hydrated oocytes were found almost exclusively within 2 h of the high tide. The association of developmental changes with changes in tidal heights points to the importance of the tidal cycle or its underlying lunar influence as a predominant reproductive cue. These data show that developmental changes in oocytes occur more rapidly than observed in some more commonly studied temperate species which reproduce annually.     

Plasma free fatty acids, inhibitor of extrathyroidal conversion of T4 to T3 and thyroid hormone binding inhibitor in patients with various nonthyroidal illnesses.

In order to clarify the role of free fatty acid (FFA) in thyroid hormone abnormalities in patients with nonthyroidal illness, thyroid function, FFA, inhibitor of extrathyroidal conversion of T4 to T3 (IEC) and thyroid hormone binding inhibitor (THBI) were studied in 99 patients with various nonthyroidal illnesses including diabetes mellitus (DM) (n = 35), liver cirrhosis (LC) (n = 33), chronic obstructive pulmonary disease (COPD) (n = 17) and chronic heart failure (CHF) (n = 14). Patients were divided into three groups based on the level of serum T3: Group I (T3 < 50 ng/dl), Group II (50 < or = T3 < 80) and Group III (80 < or = T3). Serum T4, FT3 and the T3/T4 ratio decreased significantly in the order Group III, Group II and Group I (Group III > II > I). The plasma FFA level was 0.91 +/- 0.12 mmol/l in Group I (P < 0.05, vs. Group III), 0.65 +/- 0.06 in Group II and 0.54 +/- 0.04 in Group III, respectively. The incidence of positive IEC was 80.0% in Group I (P < 0.05, vs. Group III), 53.7% in Group II (P < 0.05, vs. Group III) and 34.2% in Group III. However, IEC was not correlated with the serum T3 concentration. The incidence of positive THBI was 80% in Group I (P < 0.05, vs. Group III), 68.3% in Group II and 47.4% in Group III, but THBI was not correlated with the serum T4 level. Positive correlations were observed among FFA, IEC and THBI (P < 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)     

右美托咪定在血管外科介入手术麻醉中的应用

目的:探讨靶控输注不同浓度右美托咪定对血管外科介入手术的效果。方法:选择周围血管疾病成年患者60例,随机分为I、II、III三组,每组20例。各组右美托咪定靶控输注浓度分别为0.2μg/kg/h,0.3μg/kg/h和0.4μg/kg/h,术中监测并记录各组手术开始前(T1),手术开始10 min(T2),手术开始30 min(T3),手术开始60 min(T4)四个时间点的平均动脉压(MAP),心率(HR),血氧(Sp O2),脑电双频指数(BIS)。结果:T2、T3、T4时平均动脉压Ⅰ组与Ⅱ组比较P0.05,Ⅰ组与Ⅲ组比较P0.05。T2时Ⅲ组与Ⅰ、Ⅱ组比较P0.05,T3、T4时Ⅱ、Ⅲ组与Ⅰ组比较均P0.05。结论:右美托咪定靶控输注浓度为0.3μg/kg/h和0.4μg/kg/h时,对血管外科介入手术能产生良好的镇静作用,并且可保证术中循环的稳定。     

EFI评分、血清MMIF、YKL-40与子宫内膜异位症患者卵巢功能、临床分期的关系

摘要 目的：探讨生育指数（EFI）评分、血清巨噬细胞移动抑制因子（MMIF）、甲壳质酶蛋白 40（YKL-40）与子宫内膜异位症患者卵巢功能、临床分期的关系。方法：选取我院2020年6月到2023年6月收治的80例子宫内膜异位症患者,分为观察组,另选取同期来我院体检的80名健康女性志愿者作为对照组。对比两组受检者EFI评分、血清MMIF、YKL-40及卵巢功能指标表达水平。应用美国生殖医学协会子宫内膜异位症分期标准(r-AFS)对80例子宫内膜异位症患者进行分期,其中Ⅰ期15例,Ⅱ期23例,Ⅲ期25例,Ⅳ期17例,对比不同r-AFS分期患者EFI评分、血清MMIF、YKL-40表达水平。并采用Spearman相关法分析法分析EFI评分、血清MMIF、YKL-40与卵巢功能指标的相关性。结果：观察组患者EFI评分低于对照组,血清MMIF、YKL-40表达水平高于对照组（P＜0.05）;观察组抗苗勒氏管激素（AMH）、黄体生成素（LH）、卵泡刺激素（FSH）表达水平低于对照组,雌二醇（Estradiol,E₂）水平高于对照组（P＜0.05）;不同分期子宫内膜异位症患者EFI评分由高到低分别为Ⅰ期、Ⅱ期、Ⅲ期、Ⅳ期,MMIF和YKL-40水平由高到低分别为Ⅳ期、Ⅲ期、Ⅱ期、Ⅰ期,不同分期EFI评分、血清MMIF、YKL-40表达水平对比差异显著（P＜0.05）;Spearman相关法分析结果表明：EFI评分与AMH、LH、FSH呈正相关,与E2呈负相关（P＜0.05）,MMIF和YKL-40与AMH、LH、FSH呈负相关,与E₂呈正相关（P＜0.05）。结论：EFI评分、血清MMIF、YKL-40水平与子宫内膜异位症患者的卵巢具有明显相关性,且不同子宫内膜异位症患者EFI评分、血清MMIF、YKL-40水平具有显著差异,临床可考虑应用EFI评分、血清MMIF、YKL-40水平来辅助判断子宫内膜异位症患者的卵巢功能与疾病严重程度。     

Induction of gynogenesis and gonad development in the muskellunge

Muskellunge Esox masquinongy eggs activated with UV-irradiated yellow perch Perca flavescens sperm were subjected to heat shock at 31 ± 1° C for 6 min, 20 min after fertilization in three experiments. Survival at eyed stage was 1·7 ±1·6, 6·8 ± 4·8 and 2·3 ±0·5% in experiments I, II and III, respectively. After rearing the gynogenetic muskellunge in troughs and then in ponds, the sex ratio of gynogens in experiment I did not significantly (P>0·05) differ from the expected ratio 0:1 (male: female), however, one male and one intersex were observed. In experiments II and III, the sex ratio of gynogens differed significantly from expected (0 : 1). Three months after hatching, the growth of females did not significantly (P>0·05) differ among control and gynogenetic groups, whereas male growth was significantly (P<0·01) higher in the control v. gynogenetic group. The histological structures of the gynogenetic fish gonads in both sexes were similar to those described in the gonads of control muskellunge. After the overwintering period, signs of active spermatogenesis were observed in the testis of 1 + year old gynogenetic fish, whereas ovaries contained only oocytes at the perinucleolar stage. At this stage, plasma sex steroids testosterone (T) and oestradiol-17β (E2) cannot be used to discriminate the sex of gynogenetic muskellunge, as intersex, male and female fish had similar levels of T and E2.     

Outcome of [131I]metaiodobenzylguanidine therapy of neuroblastoma: seven years after.

Beginning in 1984 and based on a total of 40 treatments with [131I]metaiodobenzylguanidine (131I-MIBG) in most cases with a follow-up of 5 years or more, it seems to be worthwhile reevaluating our clinical data and draw some final conclusions: We treated 12 children with a neuroblastoma (NB) IV and 3 with a NB III. In no case 131I-MIBG was the primary therapy. The great majority suffered from recurrence. The mean treatment interval after chemotherapy was 6 months (range 0-54). We calculated a median cumulative tumor dose of 77 Gy (range 0-259) in patients with stage III and 30 Gy (range 4-267) in stage IV NB. The tumor half-life time of 131I-MIBG does not significantly differ between stage III (3 days) and IV (2-5 days). Although the median tumor dose of stage III NB exceeded that of stage IV, we found in NB IV a significant tumor remission in 7 out of 12 cases. On the other hand, a slight reduction of tumor size was seen in only 1 case of stage III NB. This indicates a lower radiation sensitivity of stage III NB. Despite this fact, the two patients with stage III NB who presented a sufficient 131I-MIBG-tumor uptake turned to become operable after 131I-MIBG. Stage IV patients improved, too, even if most of them suffered from recurrence with a very poor prognosis: 3 patients of stage IV lived longer than 48-60 month or are still alive. However, no one of this group remitted completely.(ABSTRACT TRUNCATED AT 250 WORDS)     

Nonfunctional overreaching during off-season training for skill position players in collegiate American football

The purpose of this study was to determine the performance and hormonal responses to a 15-week off-season training program for American football. Nine skill position players from a National Collegiate Athletic Association (NCAA) Division I-A football team participated as subjects in this study. Following 4 weeks of weight training (phase I), subjects performed weight training concurrently with high-volume conditioning drills (phase II). Phase III consisted of 15 spring football practice sessions executed over a 30-day period. Performance and hormonal changes were assessed prior to phase I, and following phases I, II, and III. Maximal strength was significantly increased (p < 0.05) for all strength tests during phase I. Squat and power clean values decreased following phase II (p < 0.05), with all values returning to baseline upon completion of phase III. Sprinting speed significantly worsened during phase I (p < 0.05), but then returned to baseline during phase III. Vertical jump and agility improved during phase I (p < 0.05), with vertical jump remaining unchanged for the duration of the study and agility returning to baseline following phase II. Testosterone levels decreased during phase II (p < 0.05) prior to returning to baseline levels during phase III. Cortisol and the testosterone/cortisol ratio remained unchanged during the course of the investigation. Even though overtraining did not occur in the current investigation, a significant maladaptation in performance did occur subsequent to phase II. For this particular athletic population, a strength and conditioning program utilizing a reduced training volume-load may prove more effective for improving performance in the future.     

血清G-CSF、ADPN在奥沙利铂联合卡培他滨治疗Ⅲ期结直肠癌患者术后辅助中的表达及其与肠道菌群失调的相关性

摘要 目的：探究III期结直肠癌手术患者接受奥沙利铂联合卡培他滨治疗后血清粒细胞集落刺激因子（G-CSF）、人脂联素（ADPN）水平的变化,分析血清G-CSF、ADPN水平与肠道菌群失调的相关性。方法：选择2021年1月至2021年12月于我院接受手术治疗的220例III期结直肠癌患者为研究对象,均对其联合应用奥沙利铂与卡培他滨进行治疗,分析治疗前后患者血清G-CSF、ADPN水平变化,将入组患者按照肠道菌群情况分为肠道正常组（n=80）、菌群失调I度组（n=90）、菌群失调II度组（n=50）,分析肠道菌群失调对入组患者治疗前后血清G-CSF、ADPN水平的影响,最后评估血清G-CSF、ADPN水平与肠道菌群失调的相关性。结果：（1）治疗后入组患者血清G-CSF、ADPN水平均较治疗前出现了明显的降低,前后比较有差异（P<0.05）;（2）治疗后菌群正常组患者血清G-CSF、ADPN水平明显低于菌群失调I度组,菌群失调I度组明显低于菌群失调II度组,各组间血清G-CSF、ADPN水平有差异（P<0.05）;（3）不同肠道菌群失调组患者肠道菌群数量存在差异,患者肠道菌群失调情况越严重,其肠球菌数量越高,乳杆菌数量越低（P<0.05）;（4）血清G-CSF、ADPN水平与乳杆菌数量呈现负相关（r=-0.872、-0.781,P<0.05）,与肠球菌数量呈现正相关（r=0.772、0.819,P<0.05）。结论：Ⅲ期结直肠癌行手术治疗患者术后联用奥沙利铂与卡培他滨可以显著降低其血清G-CSF、ADPN水平,且其降低程度与患者肠道菌群失调情况相关,提示可以考虑将调节结直肠癌患者肠道菌群作为降低患者化疗后炎性反应措施之一推广于临床。     

通脉养心丸对冠状动脉粥样硬化性心脏病伴慢性病贫血患者铁调素水平的影响

目的:探讨通脉养心丸对冠状动脉粥样硬化性心脏病(CAD)伴慢性病贫血(ACD)患者铁调素水平的影响。方法:于2015年4月-2016年6月在上海交通大学医学院附属仁济医院(南院)接受治疗的患者中选取70例年龄≥40岁的冠状动脉粥样硬化性心脏病伴慢性病贫血患者并随机分成两组,每组各35人,分别服用通脉养心丸(II组)或未服用通脉养心丸(III组)。同时选取冠心病患者不伴有慢性病贫血40人为对照组(I组),该组患者服用通脉养心丸。观察比较三组服药8周前后的血铁调素及血红蛋白、铁蛋白水平的变化。结果:服药前,II、III组的血Hepc水平均明显高于I组(P0.05)。8周治疗后,II组患者血Hepc表达明显下降,Hb水平较治疗前显著升高(P0.05),其余两组血Hepc表达与治疗前相比差异无统计学意义(P0.05);II组、III组血Hepc水平显著高于I组(P0.05),III组的血Hepc水平显著高于II组;III组患者的Hb水平明显低于I组、II组(P0.05)。结论:通脉养心丸能降低冠状动脉粥样硬化性心脏病伴慢性病贫血患者的铁调素水平,提高该类患者的血红蛋白值,对冠状动脉粥样硬化性心脏病伴慢性病贫血患者,尤其是轻度贫血患者有一定的治疗价值。