中职理实一体化会计教学研究

普通的教学模式不能适应现代职业教育的发展,现代职业教育呼唤新的教学模式"理实一体化"。在教学过程中,理论的学习与实践的教学相互结合在一起,在技能训练的同时不断地深入了解和掌握理论知识,能生动形象地进行教学,激发学生的学习热情。实行理实一体化教学需要构建会计模拟实验实训室、教师调整教学方法、加强校企合作共赢、改革教学评价方法。     

中职学校《钳工工艺学》一体化教改探索

本文总结机电专业钳工工种教学改革经验,分析在传统"三段式"课程模式中《钳工工艺学》教学存在的问题和原因,提出"实践导向、项目引领、做学结合,理实一体"的课程改革理念,就重新整合教学内容和教学模式的原则和方式进行了阐述,就开展教学团队建设给出了思路和途径。     

以职业技能为导向的“动物微生物学”课程高效课堂构建与实践

动物微生物学是高职院校畜牧兽医专业的一门重要基础课,理论性、技术性、实践性很强,其教学效果对学生后续专业的学习、实践技能的掌握有重要影响。本文从课程教学必须适应培养现代专业技术人才的要求出发,探讨了对"动物微生物学"课程教学进行改革与实践的必要性,提出要通过对教学课时的调整、授课内容的优化、教学方式的创新以及多媒体、翻转课堂、理论与实践一体化(以下简称理实一体化)等多种教学手段的综合使用,构建以职业技能为导向的"动物微生物学"课程高效课堂,努力找出一条培养具有相当的专业理论知识、一定的实践操作技能和较高的职业素养的应用型、复合型专业人才的现实途径。     

浅谈技能专业理论课程教学

现今社会,职业教育受到了越来越多的重视,如何正确传授专业课程知识也成为了我们专业教师需要不断研究的课题,以此来改进自己的业务能力。例如《电子测量仪与仪器》课程的教学过程中,仪器的正确使用是本课程的重要内容。我们在教学时就要注意自己的教学内容是否正确,能够理实结合,确保测量方法的正确性,确保没有传输给学生类似"杂草"的错误教学知识。     

鲍大鹏 研究员

正鲍大鹏,1969年12月出生,博士,三级研究员,浦江人才,上海市领军人才"后备队"。现任上海市农业科学院食用菌所副所长、遗传工程室主任,兼任国家食用菌工程技术研究中心副主任、农业部南方食用菌资源利用重点实验室副主任、中国菌物学会第七届理事会常务理事和食用真菌专业委员会主任、中国食用菌协会理事、中国农学会食用菌分会理事、《菌物学报》副主编、《菌物研究》《食用菌学报》编委。     

《食用菌栽培学》开放式实验教学的探究

开放式实验教学是将实验室面向学生开放的新型教学模式,是课堂教学的一种有效的补充和延伸。本文从生物技术专业(食用菌方向)适应社会发展与需要角度出发,结合实际教学经验及教学改革的成效,探讨了如何建立《食用菌栽培学》开放式实验教学新模式,激发学生的学习兴趣,培养学生的创新能力和实践操作技能,提高实验教学的质量。     

关于食用菌大容量栽培包技术应用分析

我国食用菌的栽培历史悠久,近年来更是出现了形式多样的食用菌栽培技术,这些技术在提高食用菌产量的同时也存在一定的缺陷。目前,在食用菌栽培领域出现了一种新型的食用菌栽培技术,叫做食用菌大容量栽培包技术,该技术的出现在很大程度上解决了目前食用菌栽培过程中的一些问题。本文在分析现有的食用菌栽培技术和技术缺陷的基础上,分析了食用菌大容量栽培包技术的优点和可行性。     

《国外食用菌》

《国外食用菌》系由上海市农业科学院情报研究所编辑出版的译刊,每年出版四期,主要为我国食用菌教学、科研人员及专业户提供国外     

欢迎订阅2018年《食用菌学报》

<正>《食用菌学报》系由国家科委批准,全国公开发行的学术性刊物。主要为食用菌教学和科研人员、生产单位的技术人员及供销外贸系统和领导机关的专业干部提供食用菌遗传育种、种质资源、设施栽培、菇房管理、病虫防治、生理生化及产后加工等方面的最新研究成果。2018年《食用菌学报》为A4     

食用菌学校企合作模块教学探索与实践

针对食用菌产业本科层次应用型高素质专业人才培养目标,分析实施食用菌学校企合作模块教学的必要性以及宁德师范学院实施的可行性,从课程体系创新、教学内容设置、现代教学手段运用、模块教学、双师型教师培养、校企合作共赢模式构建等方面,探索与实践食用菌学校企合作、模块教学的教学改革,取得了较好的效果,为推动食用菌学应用型人才培养教学模式改革提供参考。     

灵芝酸A对前列腺癌LNCaP细胞的生长抑制作用及机制

本研究利用alamarBlue~?测定细胞活力法、流式细胞术(annxin V-FITC)/PI双染色测定细胞凋亡法和高效液相色谱(high performance liquid chromatography,HPLC)测定5α-还原酶活性法评价灵芝酸A对前列腺癌的体外抗肿瘤活性,并进一步利用流式细胞术2’,7’-二氯荧光素二乙酸酯(2’,7’-dichlorofluorescindiacetate,H2DCFDA)染色测定ROS释放量法、实时荧光定量PCR(quantitative real-time PCR,qPCR)和蛋白质免疫印迹(western blot,WB)检测雄激素受体(androgen receptor,AR)基因和凋亡相关基因表达的方法,探讨其作用机理。研究结果表明灵芝酸A可通过抑制5α-还原酶活性,抑制睾酮诱导的前列腺癌LNCaP细胞增殖,诱发细胞早期凋亡来发挥抗肿瘤活性;进一步研究表明,灵芝酸A降低前列腺癌细胞中AR的表达,并通过引发细胞线粒体功能障碍释放过量活性氧(reactive oxygen species,ROS)诱发细胞凋亡,而qPCR和WB的数据进一步表明细胞线粒体功能障碍与抑癌基因caspase-3、bad和aifm1的高表达密切相关。     

Influence of hyperbaric oxygen tension on the metabolism of Candida tropicalis and Rhodococcus erythropolis

Summary The effect on metabolism of hyperbaric dissolved oxygen tension in batch cultures of Candida tropicalis (Cast.) Berkhout and Rhodococcus erythropolis with three different carbon sources was studied in a 20-l bioreactor under controlled conditions. The respiratory quotient was not significantly influenced by dissolved oxygen concentrations up to 40 mg/l oxygen. Elementary cell composition and proportional contents of DNA, RNA, and protein were not markedly influenced by the various oxygen concentrations but depended mainly on the growth rate. It was found that the production of trehalose lipid by R. erythropolis was dependent on the growth rate which could be enhanced by raising the oxygen concentration. The specific activity of catalase was affected more by the nature of the carbon source than by increased oxygen concentration. On the basis of the experimental data the application of oxygen-enriched air for biotechnological processes is discussed.Symbols and abbreviations k_La Specific volumetric oxygen transfer rate - Oxygen consumption rate, grams oxygen per hour and per liter - Carbon dioxide production rate, grams carbon dioxide per hour and per liter - RQ Respiratory quotient, - t Cultivation time - Y_X/S Yield coefficient, grams cell dry weight/grams substrate - Yield coefficient, grams cell dry weight/grams oxygen consumed - Y_kJ Yield coefficient, grams cell dry weight/heat of combustion of the consumed substrate - Y_ave– Yield coefficient, grams cell dry weight/mol available electrons of the consumed substrate     

生态学专业“五位一体”建设模式的实践探索

以华南农业大学生态学专业建设与改革实践为例,分析了生态学专业在发展过程中存在的问题,在此基础上,提出了"五位一体"的专业建设模式,即"人才培养方案--师资团队--教学平台--精品课程与教材--教学方法、模式与管理规范"全方位一体化特色专业的建设思路.同时系统总结了近年来本专业在人才培养方案修订与优化、教学团队建设、精品课程建设、教材建材、实习基地建设、实践教学改革、全程的教学方法创新等方面的相关做法与经验,旨在为其他高校的生态学专业建设提供一些借鉴与参考.     

网柄细胞状黏菌的化学成分及药理作用研究进展

文中综述了从网柄细胞状黏菌中分离得到的DIF-1及其衍生物、氯代苯并呋喃、三联苯化合物等芳香族化合物,脂肪酸和磷酸化合物、酶以及糖类等,并介绍了各成分的药理或生理作用等。表明网柄细胞状黏菌可以成为某些活性成分及药物的新来源。     

三种海水生态养殖池塘细菌数量特征及其与环境因子的相关性

为阐明不同生态养殖池塘浮游细菌数量变化规律及其与环境因子间的相关性,以期为多元立体综合养殖模式中的水质安全与生物疾病防控提供科学依据。利用荧光显微镜细菌计数法(Acridine Orange Direct Counting),研究了“参-虾”“蟹-蛏”和“蜇-蛏”三种海水生态养殖池塘的细菌数量特征,使用典范对应分析(Canonical Correspondence Analysis, CCA)探讨细菌数量与环境因子的相互关系。结果表明,“参-虾”“蟹-蛏”和“蜇-蛏”池塘的细菌平均密度分别为(0.49～3.32)×10⁵ cell/mL、(0.52～2.98)×10⁵ cell/mL和(0.47～2.55)×10⁵ cell/mL,细菌数量有明显的时间差异,夏季密度大于春秋季。三种海水生态养殖池塘的水温、溶解氧和pH变化均不明显,且相差不大,而硝态氮均发生显著变化;其中“蟹-蛏”和“蜇-蛏”池塘的硝态氮、亚硝态氮、活性磷、总氮和总磷均高于“参-虾”池塘,透明度、叶绿素a和化学耗氧量则均低于“参-虾”池塘。典范对应分析(C...     

A redescription of <Emphasis Type="Italic">Cryptocentrus crocatus</Emphasis> Wongratana,a redefinition of <Emphasis Type="Italic">Myersina</Emphasis> Herre (Acanthopterygii; Gobiidae), a key to the species,and comments on relationships

Myersina is redefined, based on the presence of an elongate first dorsal fin in at least the adult males, two preopercular pores, gill membranes fused in the ventral midline and either fused to the isthmus or forming a free fold over it, cycloid scales only on the body, and midline of predorsal region scaleless. It now includes four species traditionally assigned to Cryptocentrus but here placed in Myersina ("C". crocatus, "C". filifer, "C". pretoriusi, and "C". yangii). This concept of Myersina still retains the species originally assigned to it (M. macrostoma, M. lachneri, and M. nigrivirgata). The recently described M. larsonae belongs in Stonogobiops. These two genera almost definitely form a monophyletic group. However, it is possible that the species of Stonogobiops (the monophyly of which is well supported) could be more closely related to one or more species of Myersina than either is to other Myersina species, because the monophyly of the latter genus remains uncertain. Received: March 23, 2001 / Revised: September 7, 2001 / Accepted: October 18, 2001     

Ventilation and oxygen consumption in rosy finches and house finches at sea level and high altitude

Summary Rosy finches (Leucosticte arctoa) breed at altitudes above 3500 m in eastern California. House finches (Carpodacus mexicanus) belong to the same subfamily (Carduelinae), but breed at much lower elevations. Oxygen consumption ( ) and ventilatory parameters of these two species were measured over a wide range of ambient temperatures (T _a) at low altitude (LA; 150 m) and at high altitude (HA; 3800 m).Minimal nighttime 's of rosy finches and house finches at LA (T _a=30°C) were close to allometrically predicted values for passerine birds. At both altitudes, increased linearly with decreasingT _a betweenT _a=20 and –10°C. Resting 's were slightly higher at HA than at LA on average.In both species, minute volume ( ) was inversely related toT _a.T _a-correlated increases in resulted from significant increases in both ventilatory frequency (f) and tidal volume (V T) at both altitudes. Oxygen extraction efficiency ( ) was independent ofT _a in rosy finches at LA, but declined significantly with decreasingT _a in rosy finches at HA and in house finches at both altitudes.At a givenT _a, both species had significantly greater (BTPS) at HA than at LA. Altitude-correlated increases in resulted primarly from increases inf with little change inV T. was significantly greater at HA than at LA in both species.In spite of the difference in altitudinal distributions of rosy finches and house finches, there were few conspicuous interspecific differences in metabolic or ventilatory adaptation to altitude or lowT _a over the range of conditions examined.Symbols and abbreviations BMR basal metabolic rate - BTPS at body temperature and pressure, saturated - oxygen extraction efficiency - f ventilation frequency - h mean coefficient of heat transfer - HA high altitude - instantaneous oxygen consumption - LA low altitude - RH relative humidity - SMR standard metabolic rate - STPD standard temperature and pressure, dry - T temperature - a ambient - b body - lc lower critical of thermoneutral zone - minute volume - V T tidal volume     

Farming Eucheuma in Zanzibar,Tanzania

5. Cultivation, tissue culture

Farming Eucheuma in Zanzibar, Tanzania     

Skeletal muscle [(V)\dot]O2 \dot V_{O_2 } in rat and lemming: Effect of blood flow rate

Summary The rate of oxygen consumption ( ) by skeletal muscle was investigated in isolated perfused hindlimbs of laboratory rats and lemmings (Lemmus). In both species, increased in proportion to blood flow rate, even at flow rates 4–5 times above resting level. The slope of the line relating to skeletal muscle blood flow was significantly greater in the lemming than in the rat. This may be related to the inverse relationship between body weight and metabolic rate. These data support the hypothesis that in small animals a dependent relationship exists between blood flow and skeletal muscle .     

Age-Related Changes in Oxygen Consumption in the Edible Mussel <Emphasis Type="Italic">Mytilus edulis</Emphasis> from the White Sea

We determined the rate of oxygen consumption in the White Sea edible mussel Mytilus edulis of different ages. The mass-specific rate of oxygen consumption proved to decrease with mussel age according to the equation: = 40.1/(1 – e ^–0.194t ), where is mass-specific rate of oxygen consumption and t is age. Allometric coefficients of the oxygen consumption rate–soft tissue weight relationship were also determined.