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Najmudin S Coté ML Sun D Yohannan S Montano SP Gu J Georgiadis MM 《Journal of molecular biology》2000,296(2):613-632
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Zhiyong Shen Wubin Qu Wen Wang Yiming Lu Yonghong Wu Zhifeng Li Xingyi Hang Xiaolei Wang Dongsheng Zhao Chenggang Zhang 《BMC bioinformatics》2010,11(1):143
Background
Multiplex PCR, defined as the simultaneous amplification of multiple regions of a DNA template or multiple DNA templates using more than one primer set (comprising a forward primer and a reverse primer) in one tube, has been widely used in diagnostic applications of clinical and environmental microbiology studies. However, primer design for multiplex PCR is still a challenging problem and several factors need to be considered. These problems include mis-priming due to nonspecific binding to non-target DNA templates, primer dimerization, and the inability to separate and purify DNA amplicons with similar electrophoretic mobility. 相似文献12.
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Diagnostic polymorphisms in the mitochondrial cytochrome b gene allow discrimination between cattle,sheep, goat,roe buck and deer by PCR-RFLP 总被引:3,自引:0,他引:3