A COMPARTMENTAL ANALYSIS OF CIRCULATORY LYMPHOCYTES IN THE SPLEEN

A tentative model describing the passage of circulatory lymphocytes through the spleen is formulated in accord with known anatomical features. In order to preserve isomorphism between the model and the splenic system, the model is formulated in compartmental form and its design allows alternative routes and modes of lymphocyte transit to be considered. The simultaneous differential equations arising from the model are solved using an analogue computer which also provides the means whereby the performance of the model may be compared with suitable dynamic data drawn from literature. This not only allows the selection of a particular configuration of the model in preference to its alternatives, but also allows the numerical determination of certain unknown parameters. In the case of the rat spleen, best agreement between model and experimental data is obtained when between 10 and 25% of the total lymphocyte flux in the model spleen passes through the marginal zone where the average dwell time of the lymphocytes is about 50 min. The white pulp receives a lymphocyte flux from the marginal zone amounting to about 10% of the total splenic flux and the white pulp lymphocytes are sequestered for a period of 4–6 hr before release to the venous circulation. The red pulp receives 90% of the total splenic flux but the majority of lymphocytes find transit through the red pulp in less than 5 min. The remaining flux of lymphocytes, amounting to 10% of the splenic input, is delayed in transit through the red pulp by 2–3 hr before release to the venous circulation.