Use of a crude extract or purified antigen from first‐instar cattle grubs,Hypoderma lineatum,for the detection of anti‐Hypoderma antibodies in free‐ranging cervids from southern Spain

During the 2003–2005 hunting seasons, a total of 120 Cervidae, including 39 red deer (Cervus elaphus hispanicus) and 81 fallow deer (Dama dama), were examined for subcutaneous myiasis. Animals were shot from January to June in southern Spain. Specific antibodies against Hypodermatinae (Diptera: Oestridae) were detected by indirect enzyme‐linked immunosorbent assay (iELISA) using a crude larval extract (CLE) and a purified antigen [hypodermin C (HC)] obtained from first instars of Hypoderma lineatum (De Villers) (Diptera: Oestridae). Hypoderma actaeon Brauer was the only species detected in this study, which represents the first confirmation of this species in fallow deer from Spain. The overall prevalence of animals presenting subcutaneous larvae (14.2%) was considerably lower than the prevalences determined by iELISA with CLE (43.3%) and HC (40.0%). Red deer showed a higher prevalence of Hypoderma than fallow deer. The concordance between larval examination during the hunting season and iELISA using both antigens was low, whereas the concordance between the CLE and HC ELISAs was good. Larval antigens obtained from H. lineatum constitute a good tool for the diagnosis of H. actaeon in Cervidae, especially when the hunting season does not coincide with the maximum presence of larvae on the back.     

Bovine tuberculosis in Doñana Biosphere Reserve: the role of wild ungulates as disease reservoirs in the last Iberian lynx strongholds

Doñana National Park (DNP) in southern Spain is a UNESCO Biosphere Reserve where commercial hunting and wildlife artificial feeding do not take place and traditional cattle husbandry still exists. Herein, we hypothesized that Mycobacterium bovis infection prevalence in wild ungulates will depend on host ecology and that variation in prevalence will reflect variation in the interaction between hosts and environmental risk factors. Cattle bTB reactor rates increased in DNP despite compulsory testing and culling of infected animals. In this study, 124 European wild boar, 95 red deer, and 97 fallow deer were sampled from April 2006 to April 2007 and analyzed for M. bovis infection. Modelling and GIS were used to identify risk factors and intra and inter-species relationships. Infection with M. bovis was confirmed in 65 (52.4%) wild boar, 26 (27.4%) red deer and 18 (18.5%) fallow deer. In the absence of cattle, wild boar M. bovis prevalence reached 92.3% in the northern third of DNP. Wild boar showed more than twice prevalence than that in deer (p<0.001). Modelling revealed that M. bovis prevalence decreased from North to South in wild boar (p<0.001) and red deer (p<0.01), whereas no spatial pattern was evidenced for fallow deer. Infection risk in wild boar was dependent on wild boar M. bovis prevalence in the buffer area containing interacting individuals (p<0.01). The prevalence recorded in this study is among the highest reported in wildlife. Remarkably, this high prevalence occurs in the absence of wildlife artificial feeding, suggesting that a feeding ban alone would have a limited effect on wildlife M. bovis prevalence. In DNP, M. bovis transmission may occur predominantly at the intra-species level due to ecological, behavioural and epidemiological factors. The results of this study allow inferring conclusions on epidemiological bTB risk factors in Mediterranean habitats that are not managed for hunting purposes. Our results support the need to consider wildlife species for the control of bTB in cattle and strongly suggest that bTB may affect animal welfare and conservation.     

Copper residues in meat from wild artiodactyls hunted with two types of rifle bullets manufactured from copper

Copper content in muscle and the presence of bullet fragments were assessed in samples from red deer (Cervus elaphus), roe deer (Capreolus capreolus), fallow deer (Dama dama) and wild boar (Sus scrofa) (total of 46 animals) which had been hunted with two types of solid copper bullets. Also, the release of copper from bullets or fragments remaining in muscle was tested. For bullet type “B”, a fragment was detected in only 1 out of 34 carcasses whereas for type “A”, fragments were detected in all 12 carcasses, with up to 24 fragments (maximum size, 5?×?7?×?2 mm). Median copper concentrations around the shot wound (0–30 mm distance) were 1.25 and 1.77 mg/kg fresh weight for bullet types B and A, respectively, and thus in the expected range for venison. Around bullet fragments that had remained in muscles, the copper content increased significantly. In roe deer longissimus muscle that had been exposed for 7 days at +7 °C to bullets of type B, up to 1,000 mg/kg copper (fresh weight) was found in a distance of 0–2 mm. However, in a distance of 10–20 mm, maximum copper contents were <10 mg/kg fresh weight. Bullet fragments can constitute physical hazards and will release copper under acidic conditions as those prevailing in meat. Removal of bullet fragments prior to culinary preparation should ensure that a recommended dietary copper intake of 1.25 mg per adult consumer per day is not exceeded. From a food hygiene viewpoint, non-fragmenting bullets seem to be preferable.     

Differential long-term impacts of a management control program of axis deer and wild boar in a protected area of north-eastern Argentina

Exotic ungulates are among the top global invasive mammals and a threat to biodiversity. Axis deer (Axis axis) and wild boar (Sus scrofa) are of increasing concern in multiple regions. A management program reduced wild boar abundance and soil damage below target levels through controlled still shooting from watchtowers and dog-hunting performed by recreational hunters at El Palmar National Park, Argentina. Here we assess program impacts on axis deer over a 10-year period in which 2380 deer were dispatched, and document two largely unexpected outcomes: increasing axis deer abundance toward a plateau, and a strong inverse correlation between deer and wild boar numbers. Unlike the initial steep decline and subsequent stabilization of wild boar, deer abundance indexed by standardized catch-per-unit-effort increased at 37.6% per year over 0–5 years post-intervention (YPI) and stabilized from 7 YPI on when still-shooting effort averaged 948 hunting party-hours per quarter. Deer catch was non-linearly related to still-shooting effort. Timing of deer and boar catches did not differ significantly regardless of sex, season and YPI. Catch-per-unit-effort indices and nightly spotlight deer counts showed similarly increasing trends. The fraction of older adult deer declined over 0–4 YPI and remained stable thereafter. Sex ratios were consistently skewed toward males only among older adults. Failure to reduce deer abundance may be explained by several major processes: protracted exponential growth of the deer population after park invasion; deer regional expansion with increasing immigration; insufficient sex- and stage-biased hunting mortality, and competitor (and perhaps predator) release from wild boar.     

Movement pattern of red deer during drive hunts in Sweden

We investigated the movement pattern of GPS-collared red deer (Cervus elaphus) during drive hunts with loose dogs. In 46 flights of hinds (N?=?9), the median flight distance was 2.5 km (range 0.4–15.0). In 28 % of the flights, the hind left its home range. Average time before returning to home range was 23 h (range 2–88). Hinds in a less forested site left their home ranges more often, fled longer distances, moved at higher speed, and returned later than hinds in a homogenous forest. Speed of movement increased with number of hunts in the less forested site. In eight flights of stags (N?=?4), the median flight distance was 5.1 km (range 2.2–13.3). The start of hunting season seemed to trigger stags’ departure to their wintering areas. Knowledge of reactions to disturbance can aid game management to choose hunting methods and dogs that cause less disturbance but may also be used to deter deer from areas where they damage crops and forest plantations.     

Reconciling economic and ecological sustainability: can non-intensive hunting of red-legged partridges be economically profitable?

The red-legged partridge (Alectoris rufa) is the most important gamebird in Spain. Due to population declines in recent decades, management approaches increasingly include the release of farm-reared partridges and the establishment of so-called intensive estates, where hunting is based on put-and-take partridges. The release of farm-reared partridges is controversial because these birds negatively affect wild populations. However, according to managers, releases are necessary to maintain commercial hunting. We analysed economic parameters using data provided by 20 red-legged partridge hunting estates with different management and economic aims in central Spain. We calculated total revenues, total expenses, benefits and profitability of these estates and simulated results under two different scenarios (prices for buying farm-reared partridges being 15 % higher and prices for hunting in estates not using releases being 15 % higher) attempting to mimic the internalization of some ecological costs of farm-reared birds or possible rewards from using wild populations sustainably. Non-commercial estates (n = 5), as expected, just broke even, with almost no benefits. Benefits of intensive estates, where massive releases throughout the hunting season allow a much higher harvest, were on average an order of magnitude higher (20,404 ± 24,413 €/km², n = 8) than those of other commercial estates (1713 ± 1280 €/km², n = 7). Benefits of non-intensive commercial estates using releases were on average lower than those of commercial estates without releases. In fact, profitability of non-intensive commercial estates not using releases was similar to that of intensive estates. Any of the two price scenarios considered led to non-intensive commercial estates not using releases being the most profitable on average. Thus, efforts should be made to identify ways of improving economic profitability of non-intensive estates, which may be important for promoting hunting management styles that are ecologically more beneficial.     

The effect of treating method of game on the content of biogenic amines in wild duck (Anas platyrhynchos) meat during the course of storage

The aim of present study was to assess the correlation between the method of treating the carcasses of shot wild ducks (Anas platyrhynchos) and the formation of biogenic amines in the their muscles. The carcasses of wild ducks (n?=?180) were divided into three groups of 60 carcasses according to the method of treatment: eviscerated, drawn, and left untreated. Each group was further divided into three subgroups of 20 duck carcasses on the basis of the storage temperature (0, 7, and 15 °C) and stored for 21 days. Samples of breast and thigh muscles were taken at regular weekly intervals. Biogenic amines (cadaverine, putrescine, tyramine, histamine, phenylethylamine, and tryptamine) in samples of breast and thigh muscles were separated by reverse-phase liquid chromatography and detected by tandem mass spectrometry. The sum of biogenic amines was compared with a value of 5 mg/kg, indicating the critical content for fresh meat of high hygienic quality. The results of this study indicated that the sum of biogenic amines in wild duck meat exceeded this limit in an extremely short period of time after the commencement of storage (during the first week of storage). Higher content of biogenic amines were recorded in thigh muscle compared to breast muscle of drawn ducks and untreated ducks. According to our results, the generally recommended method for treating the carcasses of feathered game after hunting (evisceration) does not represent a method that would ensure a longer period of freshness or higher hygiene quality of the game than the other two possible methods of treatment from the biogenic amines point of view.     

Cortisol response of wild ungulates to trauma situations: hunting is not necessarily the worst stressor

Animal welfare concerns are becoming a central issue in wildlife management and conservation. Thus, we investigated stress response of wild ungulates to potentially traumatic situations (shooting injuries, vehicle collisions, entanglement, injuries or diseases) and hunting methods (stalking, battues and hunts with dogs) by means of serum cortisol concentrations from blood collected from killed animals. Cortisol levels in roe deer ranged below and in wild boar above levels for moose, red deer and fallow deer (hence, pooled as a group “deer”). Apart from species, cortisol concentration in trauma situations was mainly explained by trauma type and presence of disturbance after the trauma event. Effect of trauma type differed significantly for “deer”, with animals caught in fences and suffering vehicle collisions experiencing higher cortisol levels than animals injured by shooting. Differences between hunting methods were observed in the cervids (“deer” and roe deer), with stalking leading to lower cortisol levels than hunts with dogs (both groups) and battues (roe deer). Events both before and after the shot, such as duration of pursuit prior to shooting, location of injury, trauma length and presence of disturbance after the shot were relevant for cortisol levels in hunted cervids. Our results indicate that search teams tracking and euthanising wounded animals should behave in a calm way to minimise disturbance. Still, it is important to acknowledge that many situations described in the literature, i.e. reindeer handling, roe deer captures and red deer yarding, seem even more stressful, beside vehicle collisions, than most hunting methods.     

Comparing red deer (Cervus elaphus L.) and wild boar (Sus scrofa L.) dispersal patterns in southern Belgium

This study analyses the natal dispersal of red deer and wild boar in order to compare their dispersal capabilities in southern Belgium and to evaluate the relevance of management unit areas (MUA) designed for their monitoring. Dispersal was studied thanks to a mark-recovery method based on 111 red deer fawns and 1,613 piglets. The recovery rate of ear-tagged animals was 68 and 40 %, respectively. In both species, sub-adult males moved on longer distances ( $ \mathop{x}\limits^{-} $ red deer?=?4.82+/?4.17 km and $ \mathop{x}\limits^{-} $ wild boar?=?4.90+/?5.65 km) than females and juveniles $ \mathop{x}\limits^{-} $ (red deer?=?1.84+/?1.46 km and $ \mathop{x}\limits^{-} $ wild boar?=?2.49+/?3.74 km). Taking into account the age and sex categories, we found no difference between species in dispersal mean distance. But we observed higher maximal dispersal distances in wild boar compared to red deer. The natal home range mean sizes were 5.29 km² (+/?4.87) for red deer and 6.23 km² (+/?4.60) for wild boar. Red deer and wild boar showed similar dispersal rates according to age and sex category: 53 and 42 % in sub-adult males and 14 and 16 % in females and juveniles. Our results confirmed the higher proportion of philopatry in females and juveniles of both species compared to sub-adult males more likely to disperse. Wild boar of any sex or age seemed to be less sensitive than red deer to infrastructure (road, rail, river) network on which the management unit area limits are currently based.     

Interactive impacts of by-catch take and elite consumption of illegal wildlife

Harvesting, consumption and trade of forest meat are key causes of biodiversity loss. Successful mitigation programs are proving difficult to design, in part because anthropogenic pressures are treated as internationally uniform. Despite illegal hunting being a key conservation issue in the Pacific Islands, there is a paucity of research. Here, we examine the dynamics of hunting of birds and determine how these contribute to biodiversity loss on the islands of Samoa. We focus on the interactive effects of hunting on two key seed dispersing bird species: the Pacific pigeon (Ducula pacifica) and the critically endangered Manumea or tooth-billed pigeon (Didunculus strigiristris). We interviewed hunters, vendors and consumers and analyzed household consumption. Results suggest that over 22,000 pigeons were consumed per year and this is by primarily the richest people across the country. Indeed, the wealthiest 10% of households consumed 43% of all wild pigeon meat, and the wealthiest 40% of households consumed 80% of all pigeons. The Manumea was shot by 33% (n = 30) of the surveyed hunters while pursuing the Pacific pigeon. Results raise serious conservation concerns, as pigeon hunting is likely to be a key factor contributing to the decline of the Manumea and critical forest seed dispersers in general. Our results show that wild meat consumption can lead to non-targeted pressure on bycatch species. Wild meat harvesting and consumption is a key issue leading to species declines and extinctions in the tropics. It is critical that this issue receives the appropriate attention and is addressed in the Pacific if species and forests are to be maintained.     

Autumn-winter diet overlap of fallow, red, and roe deer in forest ecosystems, Southern Poland

The wild population of fallow deer in Central Europe has grown considerably over the last decade. However, information on feeding habits of this alien species in relation to the indigenous red deer or roe deer, in areas of their co-occurrence, is scarce. A prevailing view maintains that their food-niches are distinct, although direct comparative studies have not been carried out. Therefore, the aim of the research was to compare the diets of fallow, red, and roe deer feeding in the same habitat. Research was based on the rumen contents of 242 animals hunted in the autumn-winter season in the forests of Southern Poland. The analyses demonstrated that fallow deer are moderate grazers in such conditions and eat more graminoids in comparison to red or roe deer (36.4% vs. 16.1% or 5.5%, respectively). On the other hand, it feeds on less browse (17.2% vs. 41.4%) or dwarf shrubs (8.4% vs. 19.0%) than red deer, and on less bramble (10.9% vs. 34.6%) or forbs (4.0% vs. 7.6%) in comparison to roe deer (P=0.05). Although the diets of the three deer species differ in terms of the proportion of each food type in their diet, overlapping of their food-niches is high (52.6%).     

Nickel and copper residues in meat from wild artiodactyls hunted with nickel-plated non-lead rifle bullets

A nickel (Ni)-plated copper-solid bullet type released up to 93 μg Ni /10 g bullet mass when immersed into meat juice for 7 days (to simulate fragments remaining in venison). A non-nickel-plated counterpart of identical construction released no Ni, but up to 250 μg copper. During thermal processing of pork cubes with embedded bullets, an average of 2.8 and up to 4.3 (maximum) μg Ni were released from the Ni-plated bullet to the surrounding meat. Average nickel and copper content in meat samples (taken in 2–3 cm distance from the shot wounds) from 30 roe deer (Capreolus capreolus) and 3 sika deer (Cervus nippon) killed with nickel-plated copper bullets did not differ significantly from those in controls (roe deer haunch). Contamination scenarios would Cu and Ni contents per portion increase moderately by 20 and 3.3 μg, respectively. In order to limit alimentary Ni uptake, the technological need for Ni-plating of bullets should be carefully evaluated.     

Modern Hunting Practices and Wild Meat Trade in the Oil Palm Plantation-Dominated Landscapes of Sumatra,Indonesia

The ongoing expansion of plantation agriculture has changed the ecological, demographic, and social conditions of Southeast Asia’s forested areas, yet little is known about hunting practices in these novel landscapes. Using information from 73 in-depth interviews with hunters, agricultural workers and wild meat dealers in the Jambi province of Sumatra, Indonesia, we describe contemporary hunting practices, including how hunting methods, wildlife harvest and consumption rates vary between different indigenous and immigrant ethnic groups. Hunting is now primarily a commercial endeavor for harvesting wild boar (Sus scrofa) meat; over 7500 wild boars were sold in Jambi City alone in 2011. The Muslim majority avoids wild boar for religious reasons, but there is substantial local and export demand driven by Chinese and Christian Batak. We conclude that hunting within oil palm plantations may reduce crop damage from wild boar and also yield large amounts of wild meat with relatively little by-catch of threatened animals.     

Screening Microalgae Strains for Biodiesel Production: Lipid Productivity and Estimation of Fuel Quality Based on Fatty Acids Profiles as Selective Criteria

The viability of algae-based biodiesel industry depends on the selection of adequate strains in regard to profitable yields and oil quality. This work aimed to bioprospecting and screening 12 microalgae strains by applying, as selective criteria, the volumetric lipid productivity and the fatty acid profiles, used for estimating the biodiesel fuel properties. Volumetric lipid productivity varied among strains from 22.61 to 204.91 mg l^?1 day^?1. The highest lipid yields were observed for Chlorella (204.91 mg l^?1 day¹) and Botryococcus strains (112.43 and 98.00 mg l^?1 day^?1 for Botryococcus braunii and Botryococcus terribilis, respectively). Cluster and principal components analysis analysis applied to fatty acid methyl esters (FAME) profiles discriminated three different microalgae groups according to their potential for biodiesel production. Kirchneriella lunaris, Ankistrodesmus fusiformis, Chlamydocapsa bacillus, and Ankistrodesmus falcatus showed the highest levels of polyunsaturated FAME, which incurs in the production of biodiesels with the lowest (42.47–50.52) cetane number (CN), the highest (101.33–136.97) iodine values (IV), and the lowest oxidation stability. The higher levels of saturated FAME in the oils of Chlamydomonas sp. and Scenedesmus obliquus indicated them as source of biodiesel with higher oxidation stability, higher CN (63.63–64.94), and lower IV (27.34–35.28). The third group, except for the Trebouxyophyceae strains that appeared in isolation, are composed by microalgae that generate biodiesel of intermediate values for CN, IV, and oxidation stability, related to their levels of saturated and monosaturated lipids. Thus, in this research, FAME profiling suggested that the best approach for generating a microalgae-biodiesel of top quality is by mixing the oils of distinct cell cultures.     

Population dynamics in a guild of four Mediterranean ungulates: density‐dependence,environmental effects and inter‐specific interactions

Population fluctuations in ungulates are driven by both intrinsic and extrinsic factors. Available information, however, mainly refers to arctic, temperate and African ungulate populations, while the dynamics of Mediterranean species, exposed to a milder climate, is known to a much lesser extent. Here we studied the population dynamics of four wild ungulate species in the Castelporziano Preserve near Rome, Italy, as obtained from detailed bag counts from hunting drives during the period 1878–1986: the Italian roe deer Capreolus capreolus italicus, the Maremma wild boar Sus scrofa majori (both endemic to Italy), the native red deer Cervus elaphus, and the alien fallow deer Dama dama. We also considered the effects of the presence of another alien ungulate, the nilgai Boselaphus tragocamelus. This ungulate community experienced an accidental ‘removal experiment’ when, during World War II, red deer and nilgai were exterminated. This event and the length of the time series allowed us to test two main hypotheses: 1) that the complexity level of the ungulate community affects the strength of intra‐ and inter‐specific competition; and 2) that in Mediterranean environments intra‐ and inter‐specific interactions are stronger than climate forcing. Statistical methods ranged from state‐space‐modelling, GLM analysis and structural equation models. The results indicated that direct intra‐specific density dependence played a relevant role for all species, and was stronger after the removal. A complex pattern of species interactions was however revealed; fallow deer had a negative effect on roe deer population, while roe deer had an apparent positive effect on red deer and wild boar, possibly mediated by environmental factors. Nilgai appeared to facilitate all deer species. The results of the analysis also confirmed that at present climate appears to play a minor role with respect to density dependence; however, the increasing aridity of the Mediterranean area could change this picture in coming decades.     

Faecal pellet group counting as a promising method of wild boar population density estimation

The wild boar is a native animal species of the Czech Republic that has significantly increased its population density in recent years. Objective methods of population density estimation are required to inform the intensity of regulation needed for the sustainable management of the wild boar. Population estimation and regulation of the abundance of the wild boar are problematic in comparison to those of other ungulates, and the only widely used method of control is intensive hunting. The purpose of this study was to test if faecal pellet group (FPG) counting was a reliable method of wild boar population censusing in a forest environment. The density of wild boars was evaluated in an area of 22.56 km², circumscribed by both natural and man-made barriers that restrict wild boar migration. As it is essential for proper application of FPG count methods, a separate trial was carried out to determine the daily defecation rate of wild boars. The distribution of faeces in different types of habitat in a forest environment was tested. As the period of faeces accumulation fell within the intensive hunting season, the result is biased by the fact that the calculation includes FPGs produced by animals removed from the population through hunting during winter. The average daily defecation rate of adults and piglets was 4.29 and 5.82, respectively. The corrected (without shot animals) average density of wild boars in the study area estimated by the FPG count at the time of the census was 6.08 individuals (ind.)/km². We have confirmed that an FPG count conducted in winter is applicable for estimation of wild boar abundance on a forested area.     

Improving of lipid productivity of the biodiesel promising green microalga <Emphasis Type="Italic">Chlorella pyrenoidosa</Emphasis> via low-energy ion implantation

High lipid content in microalgae is an essential parameter for adopting of microalgal biomass as a feedstock for biodiesel. Mutation is one approach to obtain desired algal strain with high lipid production. In this study, a mutant strain of Chlorella pyrenoidosa was isolated using 1.5?×?10¹⁵ ions cm^?2 s^?1 of N⁺ ion beam implantation technique, which has been widely used in mutagenesis of agricultural crops. N⁺ implantation slightly improved the growth of the mutant over the corresponding wild strain with significant increase in lipid content (32.4 % higher than the wild strain), which resulted in significant increase in lipid productivity by 35 %. In addition, ion implantation mutagenesis of C. pyrenoidosa resulted in 21.4 % decrease in total saturated fatty acids (SFAs) compared to the wild type, with a noticeable increase in polyunsaturated fatty acids (PUFAs). The increase in PUFAs was due mainly to stimulation of hexadecadienoic acid (C16:2) and octadecadienoic acid (C18:2) production. However, the SFA content of wild and mutant strains was 31.7 and 24.9 % of total fatty acids, respectively, highlighting the oxidative stability of biodiesel produced by both strains according to the European standards. Cultivation of C. pyrenoidosa mutant in selenite enrichment medium for five successive cultivation experiments showed insignificant changes in biomass productivity, lipid content, and lipid productivity alongside the study period, which confirms the genetic stability of the produced mutant. The present study confirmed the feasibility of generation of microalgae mutants with significant high lipid production using ion beam implantation.     

Enhanced plasmid DNA production by enzyme-controlled glucose release and an engineered <Emphasis Type="Italic">Escherichia coli</Emphasis>

Objectives

To evaluate the combination of a culture medium employing glucoamylase-mediated glucose reléase from a gluco-polysaccharide and an E. coli strain engineered in its glucose transport system for improving plasmid DNA (pDNA) production.

Results

The production of pDNA was tested using E. coli DH5α grown in shake-flasks and the recently developed VH33 Δ(recA deoR)-engineered strain, which utilizes glucose more efficiently than wild type strains. Three glucoamylase concentrations for releasing glucose from the polysaccharide carbon source were used: 1, 2 and 3 U l^?1. Both strains reached similar cell densities ranging from 5 to 8.8 g l^?1 under the different conditions. The highest pDNA yields on biomass (Y_pDNA/X) for both strains were obtained when 3 U enzyme l^?1were used. Under these conditions, 35 ± 3 mgof pDNA l^?1 were produced by DH5α after 24 h of culture. Under the same conditions, the engineered strain produced 66 ± 1 mgpDNAl^?1 after 20 h. pDNA supercoiled fractionswere close to 80 % for both strains.

Conclusions

The pDNA concentration achieved by the engineered E. coli was 89 % higher than that of DH5α. The combination of the engineered strain and enzyme-controlled glucose release is an attractive alternative for pDNA production in shake-flasks.

     

Maize productivity and nutrient dynamics in maize-fallow rotations in western Kenya

One-season fallows with legumes such as Crotalaria grahamiana Wight & Arn. and phosphorus (P) fertilization have been suggested to improve crop yields in sub-Saharan Africa. Assessing the sustainability of these measures requires a sound understanding of soil processes, especially transformations of P which is often the main limiting nutrient. We compared plant production, nitrogen (N) and P balances and selected soil properties during 5.5 years in a field experiment with three crop rotations (continuous maize, maize-crotalaria and maize-natural fallow rotation) at two levels of P fertilization (0 and 50 kg P ha^?1 yr^?1, applied as triple superphosphate) on a Kandiudalfic Eutrudox in western Kenya. The maize yield forgone during growth of the crotalaria fallow was compensated by higher post-fallow yields, but the cumulative total maize yield was not significantly different from continuous maize. In all crop rotations, P fertilization doubled total maize yields, increased N removal by maize and remained without effect on amounts of recycled biomass. Crotalaria growth decreased in the course of the experiment due to pest problems. The highest levels of soil organic and microbial C, N and P were found in the maize-crotalaria fallow rotation. The increase in organic P was not accompanied by a change in resin-extractable P, while H₂SO₄-extractable inorganic P was depleted by up to 38 kg P ha^?1 (1% of total P) in the 0–50 cm layer. Microbial P increased substantially when soil was supplied with C and N in a laboratory experiment, confirming field observations that the microbial biomass is limited by C and N rather than P availability. Maize-legume fallow rotations result in a shift towards organic and microbial nutrients and have to be complemented by balanced additions of inorganic fertilizers. Abbreviations: BNF – biological nitrogen fixation; COM – continuous maize; LR – long rainy season; MCF – maize-crotalaria fallow rotation; MNF – maize-natural fallow rotation; SR – short rainy season; TSP – triple superphosphate.     

The association of lipoprotein lipase PvuII polymorphism and niacin intake in the prevalence of metabolic syndrome: a KMSRI-Seoul study

Lipoprotein lipase (LPL) polymorphism correlated with LPL activity is associated with plasma lipid and lipoprotein levels. We aimed to investigate the frequency of LPL PvuII polymorphism and effects of LPL PvuII polymorphism and niacin intake on the prevalence of metabolic syndrome (MetSyn) in Koreans. Lifestyle questionnaires, anthropometry, and dietary records were completed, and LPL PvuII polymorphism, LPL mass, and lipid profiles were determined in 548 Koreans (MetSyn: 278, Non-MetSyn: 270). The MetSyn group showed a significantly lower frequency of P1P1 (wild type) and a higher frequency of P1P2 (hetero type) than the non-MetSyn group. The P2P2 (mutant type) group significantly showed lower levels of HDLc and LPL mass and a higher level of TG than the P1P1 group. As niacin intake increased, LPL mass decreased in the P2P2 group (r ² = 0.07). In particular, the lowest niacin intake group (≤14.82 mg/day) increased more than 3 times with regard to a higher risk of MetSyn than the others in the P2P2 mutant groups. However, the MetSyn risk declined 74% at the optimal levels of niacin intake (14.83–17.80 mg/day) in the P2P2 group compared to those of the P1 allele group. The findings indicate that optimal levels of niacin intake effectively decreased Korean MetSyn prevalence in the P2P2 mutant group.