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1.
《Phytochemistry》1986,25(6):1351-1353
From the crude drug zedoary (the dried rhizome of Curcuma zedoaria), three novel sesquiterpenoids, zedoarol (a furanoguaiane), 13-bydroxygermacrone (a germacrane) and curzeone (a furanocadinane), have been isolated and their structures elucidated by extensive spectral analysis. 相似文献
2.
Curcuma zedoaria rhizome (Zingiberaceae) is a well-known traditional medicinal plant used in Ayurvedic and traditional Chinese medicine to treat various cancers. This study aimed to identify the cytotoxic components from C. zedoaria rhizomes that act against gastric cancer, which is the third leading cause of death from cancer worldwide because the MeOH extract of C. zedoaria rhizome was found to show a cytotoxic effect against gastric cancer AGS cells. Repeated column chromatography and semi-preparative HPLC purification were used to separate the components from the C. zedoaria MeOH extract. Two new sesquiterpenes, curcumenol-9,10-epoxide (1) and curcuzedoalide B (2), and 12 known related sesquiterpenes (3–14) were isolated from the C. zedoaria MeOH extract. The structures of new compounds were determined by 1D and 2D NMR spectroscopic experiments and HR-ESIMS, and quantum chemical ECD calculations. The cytotoxic effects of the isolated compounds were measured in human gastric cancer AGS cells using an MTT cell viability assay. Compounds 9, 10, and 12 exhibited cytotoxic effects against gastric cancer AGS cells, with IC50 values in the range of 212–392 μM. These findings provide further experimental scientific evidence to support the traditional use of C. zedoaria rhizomes for the treatment of cancer. Curcumenol (9), 4,8-dioxo-6β-methoxy-7α,11-epoxycarabrane (10), and zedoarofuran (12) were identified as the main cytotoxic components in C. zedoaria rhizomes. 相似文献
3.
Nguyen Hoang Loc Vo Chau Tuan Doan Huu Nhat Binh Truong Thi Bich Phuong Tae-Geum Kim Moon-Sik Yang 《Biotechnology and Bioprocess Engineering》2009,14(5):619-624
We developed a cell suspension culture system for zedoary (Curcuma zedoaria Roscoe), using 100 g fresh weight inoculum in a batch culture. The maximum cell biomass of 68.46 g/L fresh weight was obtained
after 14 days of culture in a 10 L bioreactor with a pitch-blade impeller maintained at an agitation speed of 150 rpm and
an aeration rate of 2.5 L/min. The accumulation of sesquiterpenes and polysaccharide in zedoary cells from 2 to 18 days was
measured by HPLC and a phenol-sulfuric acid assay, respectively. The total polysaccharide concentration increased between
2 to 10 days of culture and reached a maximum value of 6.55%. HPLC revealed several eluted peaks of sesquiterpenes, which
increased in amplitude from days 2 to 10. Furthermore, our results indicated that biotransformation occurred in the cell suspension,
transforming certain sesquiterpenes into other types during culture. 相似文献
4.
A new guaiane, zedoarondiol, was isolated from fresh rhizomes of Curcuma zedoaria and its structure was elucidated from spectral evidence. 相似文献
5.
In this study, a cell suspension culture system for zedoary (Curcuma zedoaria Roscoe) was developed, using 50 g/l of fresh weight inoculum in a batch culture. The highest cell biomass obtained from a
5-l bioreactor equipped with three impellers after 14 days of culture was utilized to extract secondary metabolites (essential
oil and curcumin) and determine the activities of antioxidant enzymes (peroxidase, superoxide dismutase, and catalase). For
essential oil and curcumin, zedoary extracts were recovered via a variety of methods: steam distillation, volatile solvents,
and Soxhlet. After 14 days of culture using volatile solvents, the optimal yield of essential oil (1.78%) was obtained when
using petroleum ether at 40°C in 6 h of extraction, and the best curcumin yield (9.69%) was obtained at 60°C in 6 h via extraction
with 90% ethanol. The activities of antioxidant enzymes from zedoary cells were also assessed. The specific activities of
peroxidase, superoxide-dismutase, and catalase reached maximum values of 0.63 U/mg of protein, 16.60 U/mg of protein, and
19.59 U/mg of protein after 14 days of culture, respectively. 相似文献
6.
Raju Bharalee Anita Das M. C. Kalita 《Journal of plant biochemistry and biotechnology.》2005,14(1):61-63
Two species of Curcuma (C. caesia and C. zedoaria) have been propagated through tissue culture using rhizome bud explant. The best response for shoot multiplication was obtained on MS basal medium supplemented with 4 mg l?1 BAP and 1.5 mg l?1 NAA for C. caesia (3.5 ± 0.79 shoots per explant) and 1 mg l?1 BAP + 0.5 mg l?1 NAA for C. zedoaria (4.5 ± 0.15 shoots per explant). A maximum of 9.2 ± 0.15 and 8.9 ± 0.09 roots per explant were obtained for C. caesia and C. zedoaria, respectively when MS was supplemented with 0.5 mg l?1 IAA. The rooted plants could be established in soil. 相似文献
7.
Inflammation is not only a self-defense response of the innate immune system, but also the pathogenesis mechanism of multiple diseases such as arthritis, neurodegeneration, and cancer. Curcuma zedoaria Roscoe (Zingiberaceae), an indigenous plant of India, has been used traditionally in Ayurveda and folk medicine. As part of our ongoing efforts to screen traditional medicinal plants exhibiting pharmacological potential and to characterize the compounds involved, we examined the anti-inflammatory effects of the MeOH extract of C. zedoaria rhizomes using lipopolysaccharide (LPS)-stimulated RAW264.7 murine macrophage cells and found that MeOH extract inhibited the synthesis of nitric oxide (NO) in a dose-dependent manner (IC50: 23.44 ± 0.77 μg/mL). In our efforts to characterize the compounds responsible for these anti-inflammatory effects, bioactivity-guided fractionation of the MeOH extract and chemical investigation of its active hexane-soluble fraction led to the successful isolation of five sesquiterpenes (1–5), the structures of which were elucidated by NMR spectroscopic analysis and LC/MS analysis. Among them, curcuzedoalide (5) exhibited potent inhibitory effects on NO synthesis (IC50: 12.21 ± 1.67 μM) and also suppressed pre-inflammatory protein expression of iNOS and COX-2. Curcuzedoalide (5) was thus determined to be a contributor to the anti-inflammatory effect of C. zedoaria rhizomes and could be a potential candidate for therapeutic applications. 相似文献
8.
G. R. Angel T. Makeshkumar C. Mohan B. Vimala B. Nambisan 《Journal of plant biochemistry and biotechnology.》2008,17(2):173-176
The genetic variability in starchy Curcuma species was assessed using Random Amplified Polymorphic DNA (RAPD) technique. The RAPD pattern generated by 20 primers revealed a high degree of polymorphism. A total of 274 bands were generated of which 264 were polymorphic. All the species were separated into 3 clusters using UPGMA. C. aromatica, C. leucorrhiza, and C. brog formed a cluster within which C. longa and C. zedoaria formed a subgroup. C. harita was genetically distinct from all the other Curcuma species. Since it is difficult to distinguish different species by leaf morphology, the RAPD pattern has high utility in identification of starchy Curcuma species. 相似文献
9.
Tissue culture propagation system was developed for zedoary (Curcuma zedoaria Roscoe), a valuable medicinal plant, using rhizome sprout cultures. Shoots were induced from rhizomes on basal MS medium containing 20 g l–1 sucrose and 5 g l–1 agar, supplemented with 20 (v/v) coconut water (CW) and benzylaminopurine (BA) concentrations from 0.5 to 5.0 m g l–1. The excised shoots were subcultured on Murashige-Skoog (MS) medium with 20 (v/v) CW and different concentrations of BA and kinetin (Kin), either alone or in combination with indolebutyric acid (IBA) or naphthaleneacetic acid (NAA). MS medium with 20 (v/v) CW, 3 mg l–1 BA, and 0.5 mg l–1 IBA resulted in a multiplication rate per shoot; 5.6 shoots per explant were obtained on average after 30 days of culture. Well-developed shoots (30–40 mm in length) were rooted on MS medium containing 20 g l–1 sucrose and 8 g l–1 agar, supplemented with 20 (v/v) CW and 2 mg l–1 NAA. More than 95 of the rooted plants were established in pots after hardening. 相似文献
10.
Raj Kumar Joshi Satyabrata Nanda Ellojita Rout Basudeba Kar Pradeep Kumar Naik Sanghamitra Nayak 《Bioinformation》2013,9(11):560-564
Plant NBS-LRR R-genes recognizes several pathogen associated molecular patterns (PAMPs) and limit pathogen infection through
a multifaceted defense response. CzR1, a coiled-coil-nucleotide-binding-site-leucine-rich repeat R-gene isolated from Curcuma
zedoaria L exhibit constitutive resistance to different strains of P. aphanidermatum. Majority of the necrotrophic oomycetes are
characterized by the presence of carbohydrate PAMPs β-glucans in their cell walls which intercat with R-genes. In the present
study, we predicted the 3D (three dimensional) structure of CzR1 based on homology modeling using the homology module of
Prime through the Maestro interface of Schrodinger package ver 2.5. The docking investigation of CzR1 with β-glucan using the
Glide software suggests that six amino acid residues, Ser186, Glu187, Ser263, Asp264, Asp355 and Tyr425 act as catalytic residues
and are involved in hydrogen bonding with ligand β-(1,3)-D-Glucan. The calculated distance between the carboxylic oxygen atoms
of Glu187–Asp355 pair is well within the distance of 5Å suggesting a positive glucanase activity of CzR1. Elucidation of these
molecular characteristics will help in in silico screening and understanding the structural basis of ligand binding to CzR1 protein
and pave new ways towards a broad spectrum rhizome rot resistance development in the cultivated turmeric. 相似文献
11.
A new species of pandalid shrimp Anachlorocurtis occidentalis
sp. n., associated with antipatharian corals, is described and illustrated from the north-eastern Red Sea. This new species is closely related to Anachlorocurtis commensalis Hayashi, 1975, the only other species in the genus, and can be distinguished by the more slender body and appendages; the carapace with 3 large, and one small, subtriangular lobes in the middorsal line; a flattened dorsal outline of the third abdominal segment; the sixth abdominal segment twice as long as fifth one; propodi of the ambulatory pereiopods bearing only a single posterior spinule; and harbouring 3–5 pairs of dorsolateral spines on the telson. A revised generic diagnosis is provided here to accommodate the present new species. The genetic divergence of mitochondrial gene cytochrome c oxidase subunit I (COI) between Anachlorocurtis occidentalis sp. n., and A. commensalis is 15.2–15.4%. Molecular analysis also confirmed a sister position of the genus Anachlorocurtis to Miropandalus. The present records of A. commensalis from Taiwan constitute an extension of the known range of the species. 相似文献
12.
A new species of the genus Monoliropus belonging to the family Caprellidae was collected from the Yellow Sea, Korea. The new species differs from Monoliropus
agilis Mayer, 1903, Monoliropus
kazemii Momtazi & Sari, 2013, and Triprotella
amica Arimoto, 1970 as follows: 1) gnathopod 1 subrectangular; 2) pereonites 2–3 with acute triangular processes anterolaterally; 3) mandibular palp, apical article with four simple setae subdistally. The new species is fully illustrated and extensively compared with related species. This is the first record of the genus Monoliropus from Korean waters. A key to Monoliropus species is also given. 相似文献
13.
《Lichenologist (London, England)》2001,33(2):97-102
Abstract:A new corticolous lichen, Rinodina turfaceoides, is described from the Serra de Estrela (Portugal). It is characterized mainly by the blastidiate thallus, large apothecia covered by a thick, yellow-orange pruina, large Physcia -type ascospores and chemistry. The new species is compared with the closely related but mainly muscicolous, R. turfacea, as well as with other corticolous Rinodina species with vegetative propagules, including R. colobinoides and R. disjuncta. Habit photographs of the new species and illustrations of its ascospores are also provided. 相似文献
14.
A. G. Moseyko 《Entomological Review》2012,92(7):773-781
A review of species of the genus Aphilenia is given. The genera Aphilenia and Atomyria are transferred from the tribe Bromiini to the tribe Nodinini. A new species, Aphilenia astakhovi sp. n. from Russia (Astrakhan Prov.), is described. Aphilenia interrupta var. unicolor Rtt. is upgraded to a species and is transferred to the subgenus Pseudaphilenia Lop. Aphilenia hauseri Wse. is a new synonym of A. unicolor Rtt.; A. inornata Chen is transferred to the genus Chloropterus. 相似文献
15.
A new species of the genus Aphis with stridulatory apparatus, Aphis gnetuma Qiao sp. n., feeding on Gnetum parvifolium which is a new host plant record for aphids from Guangxi, China is described. The new species is distinguished by a well-developed median frontal tubercle and body bearing white wax powder in life. The taxonomic position of this new species is supported by analysis results of COI and gnd genes. A key to Aphis species with stridulatory spines on the hind tibiae of apterous viviparous females is provided. The type specimens studied are deposited in the National Zoological Museum of China, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.www.zoobank.org/urn:lsid:zoobank.org:act:BBB024DA-8454-4E22-8736-66ED92A3091B. 相似文献
16.
Viacheslav Spirin Otto Miettinen Jorma Pennanen Heikki Kotiranta Tuomo Niemelä 《Mycological Progress》2013,12(1):53-61
A new polypore species, Antrodia hyalina, is described from Russia. It is morphologically similar to Antrodia pulvinascens, but differs in having annual, thinner and softer basidiocarps, solid skeletal hyphae, and cylindrical spores. Antrodia leucaena, originally described from China, is reported as new from Finland and Russia on Populus tremula. Antrodia wangii is regarded as a synonym of A. bondartsevae. 相似文献
17.
A new species of Nicon Kinberg, 1866 from the east Pacific coast of Ecuador is described. The new species is characterized by a long, thin dorsal ligule on median and posterior parapodia and infracicular sesquigomph falcigers in the neuropodia. A key to all species of Nicon is provided. 相似文献
18.
This work treats species of the genus Microsternus Lewis, 1887 from Asia and North America. A new genus is described: Neosternus (type species Microsternus higonius Lewis, 1887). A new species is described: Microsternus pengzhongi. A new synonym is provided: Microsternus tricolor taiwanicus Nakane (=Microsternus tricolor Lewis). Three species previously placed in Microsternus Lewis, 1887 are transferred to Neosternus resulting in the following three new combinations: Neosternus higonius (Lewis, 1887), Neosternus taiwanus (Chûjô, 1976), and Neosternus hisamatsui (Nakane, 1981). 相似文献
19.
A new genus and species, Bambusimukaria
quinquepunctata
gen. & sp. n., feeding on bamboo in Guizhou and Fujian, China, are described and illustrated. The characters of crown, frontoclypeus, forewing venations and male genitalia place the new genus in the tribe Mukariini. 相似文献
20.
Adrienne Jochum Rajko Slapnik Marian Kampschulte Gunhild Martels Markus Heneka Barna Páll-Gergely 《ZooKeys》2014,(410):23-40
A review of the microgastropod genus Systenostoma is provided. Thai and Malaysian species are transferred to a new genus, Angustopila (type species: Systenostoma tamlod Panha & Burch, 1999). A new subterranean Angustopila species is described here. Conchologically, the new species is most similar to the cave-dwelling, Thai A. tamlod (Panha & Burch, 1999). One Thai species (Systenostoma edentata) is transferred to the genus Hypselostoma. Vietnamese members remain in the genus Tonkinospira (nomen novum) for Systenostoma Bavay & Dautzenberg, 1908 (non Systenostoma Marsson, 1887). A comprehensive map of former Systenostoma species is presented. SEM and NanoCT images, including a video of A. huoyani
sp. n. internal shell morphology, provide novel perspectives of the shells of Angustopila and of the scarcely known Vietnamese Tonkinospira species. The biology of these snails is not yet known but collection localities suggest a troglophilic ecology. 相似文献