共查询到20条相似文献,搜索用时 46 毫秒
1.
2.
3.
4.
5.
X Wang D Kopinke J Lin AD McPherson RN Duncan H Otsuna E Moro K Hoshijima DJ Grunwald F Argenton CB Chien LC Murtaugh RI Dorsky 《Developmental cell》2012,23(3):624-636
Previous studies have raised the possibility that Wnt?signaling may regulate both neural progenitor maintenance and neuronal differentiation within a single population. Here we investigate the role of Wnt/β-catenin activity in the zebrafish hypothalamus and find that the pathway is first required for the proliferation of unspecified hypothalamic progenitors in the embryo. At later stages, including adulthood, sequential activation and inhibition of Wnt activity is required for the differentiation of neural progenitors and negatively regulates radial glia differentiation. The presence of Wnt activity is conserved in hypothalamic progenitors of the adult mouse, where it plays a conserved role in inhibiting the differentiation of radial glia. This study establishes the vertebrate hypothalamus as a model for Wnt-regulated postembryonic neural progenitor differentiation and defines specific roles for Wnt signaling in neurogenesis. 相似文献
6.
7.
8.
9.
10.
Impaired generation of mature neurons by neural stem cells from hypomorphic Sox2 mutants 总被引:1,自引:0,他引:1
Cavallaro M Mariani J Lancini C Latorre E Caccia R Gullo F Valotta M DeBiasi S Spinardi L Ronchi A Wanke E Brunelli S Favaro R Ottolenghi S Nicolis SK 《Development (Cambridge, England)》2008,135(3):541-557
11.
12.
Li H Jin G Qin J Tian M Shi J Yang W Tan X Zhang X Zou L 《Histochemistry and cell biology》2011,136(5):515-526
During the central nervous system (CNS) development, radial glia cells (RGCs) play at least two essential roles, they contribute
to neuronal production and the subsequent guidance of neuronal migration, whereas its precise distribution and contribution
to cerebral cortex remains less understood. In this research, we used Vimentin as an astroglial marker and Sox2 as a neural
progenitor marker to identify and investigate RGCs in rat cerebral cortex at embryonic day (E) 16.5. We found that the Sox2+
progenitor cells localized in the germinal zone (GZ) of E16.5 cerebral cortex, ~95% Sox2+ cells co-localized with Vimentin+
or Nestin+ radial processes which extended to the pial surface across the cortical plate (CP). In vitro, we obtained RG-like
cells from E16.5 cerebral cortex on adherent conditions, these Sox2+ Radial glia (RG)-like cells shared some properties with
RGCs in vivo, and these Sox2+ RG-like cells could differentiate into astrocytes, oligodendrocytes and presented the radial
glia—neuron lineage differentiation ability. Taken together, we identified and investigated some characterizations and properties
of Sox2+ RGCs derived from E16.5 cerebral cortex, we suggested that the embryonic Sox2+ progenitor cells which located in
the cortical GZ were mainly composed of Sox2+ RGCs, and the cortex-derived Sox2+ RG-like cells displayed the radial glia—neuron
lineage differentiation ability as neuronal progenitors in vitro. 相似文献
13.
14.
15.
Kan L Israsena N Zhang Z Hu M Zhao LR Jalali A Sahni V Kessler JA 《Developmental biology》2004,269(2):580-594
16.
17.
Li Y Wang J Zheng Y Zhao Y Guo M Li Y Bao Q Zhang Y Yang L Li Q 《生物化学与生物物理学报(英文版)》2012,44(8):660-668
Neural precursor cells play important roles in the neocortical development, but the mechanisms of neural progenitor proliferation, neuronal differentiation, and migration, as well as patterning are still unclear. Sox11, one of SoxC family members, has been reported to be essential for embryonic and adult neurogenesis. But there is no report about the roles of Sox11 in corticogenesis. In order to investigate Sox11 function during cortical development, loss of function experiment was performed in this study. Knockdown of Sox11 by Sox11 siRNA constructs resulted in a diminished neuronal differentiation, but enhanced proliferation of intermediate progenitors. Accompanied with the high expression of Sox11 in the postmitotic neurons, but low expression of Sox11 in the dividing neural progenitors, all the observations indicate that Sox11 induces neuronal differentiation during the neocortical development. 相似文献
18.
REN: a novel,developmentally regulated gene that promotes neural cell differentiation 总被引:7,自引:0,他引:7
下载免费PDF全文
![点击此处可从《The Journal of cell biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Gallo R Zazzeroni F Alesse E Mincione C Borello U Buanne P D'Eugenio R Mackay AR Argenti B Gradini R Russo MA Maroder M Cossu G Frati L Screpanti I Gulino A 《The Journal of cell biology》2002,158(4):731-740
Expansion and fate choice of pluripotent stem cells along the neuroectodermal lineage is regulated by a number of signals, including EGF, retinoic acid, and NGF, which also control the proliferation and differentiation of central nervous system (CNS) and peripheral nervous system (PNS) neural progenitor cells. We report here the identification of a novel gene, REN, upregulated by neurogenic signals (retinoic acid, EGF, and NGF) in pluripotent embryonal stem (ES) cells and neural progenitor cell lines in association with neurotypic differentiation. Consistent with a role in neural promotion, REN overexpression induced neuronal differentiation as well as growth arrest and p27Kip1 expression in CNS and PNS neural progenitor cell lines, and its inhibition impaired retinoic acid induction of neurogenin-1 and NeuroD expression. REN expression is developmentally regulated, initially detected in the neural fold epithelium of the mouse embryo during gastrulation, and subsequently throughout the ventral neural tube, the outer layer of the ventricular encephalic neuroepithelium and in neural crest derivatives including dorsal root ganglia. We propose that REN represents a novel component of the neurogenic signaling cascade induced by retinoic acid, EGF, and NGF, and is both a marker and a regulator of neuronal differentiation. 相似文献
19.
Wnt3a Promotes Hippocampal Neurogenesis by Shortening Cell Cycle Duration of Neural Progenitor Cells
Yutaka Yoshinaga Tetsushi Kagawa Takeshi Shimizu Toshihiro Inoue Shinji Takada Jun-ichi Kuratsu Tetsuya Taga 《Cellular and molecular neurobiology》2010,30(7):1049-1058
The effects of Wnt signaling on neural progenitor cells have been controversial. Activation of the canonical Wnt signaling
pathway either promotes neural progenitor cell proliferation or accelerates their differentiation into postmitotic neurons.
This study demonstrates that activation of the Wnt signaling pathway by itself induces neural progenitor cell proliferation
but does not directly affect neuronal differentiation processes. To investigate whether Wnt signaling promotes expansion and/or
differentiation of neural progenitor cells in the developing hippocampus, we prepared primary mouse hippocampal progenitors
and treated them with Wnt3a in a chemically defined culture medium. Wnt3a increased the total number of cells, including the
numbers of Ki67+ proliferating cells and Tuj1+ differentiated neurons. This result verified that Wnt3a promoted neural progenitor cell proliferation. Meanwhile, Wnt3a did
not appear to actively enhance the neuronal differentiation process itself, because (1) the ratio of Tuj1+ cells to the total cells, and (2) the ratio of BrdU+ Tuj1+ cells to the total BrdU+ cells, were both comparable between cultures with or without Wnt3a. Indeed, Wnt3a caused no significant change in either
cell survival or the proportion of symmetric and asymmetric cell divisions that directly affected neuron production. We finally
demonstrated that the Wnt3a treatment simply shortened cell cycle duration of neural progenitor cells by 2.9 h. The accelerated
cell cycle progression without affecting the ratio of symmetric/asymmetric cell divisions explains how Wnt signaling per se
leads to the expansion of both proliferative cell population and differentiated neuronal cell population. 相似文献
20.
Regulation of progenitor cell fate determines the numbers of neurons in the developing brain. While proliferation of neural progenitors predominates during early central nervous system (CNS) development, progenitor cell fate shifts toward differentiation as CNS circuits develop, suggesting that signals from developing circuits may regulate proliferation and differentiation. We tested whether activity regulates neurogenesis in?vivo in the developing visual system of Xenopus tadpoles. Both cell proliferation and the number of musashi1-immunoreactive progenitors in the optic tectum decrease as visual system connections become stronger. Visual deprivation for 2?days increased proliferation of musashi1-immunoreactive radial glial progenitors, while visual experience increased neuronal differentiation. Morpholino-mediated knockdown and overexpression of musashi1 indicate that musashi1 is necessary and sufficient for neural progenitor proliferation in the CNS. These data demonstrate a mechanism by which increased brain activity in developing circuits decreases cell proliferation and increases neuronal differentiation through the downregulation of musashi1 in response to circuit activity. 相似文献