He—Ne激光照射离体鼠巨噬细胞对线粒体跨膜电势的影响

目的：研究Ｈｅ－Ｎｅ激光照射鼠巨噬细胞对线粒体跨膜电势的影响,及其与激光剂量的关系。方法：用亲脂性阳离子荧光染料Ｒｈｏｄａｍｉｎｅ１２３对鼠巨噬细胞线粒体作荧光标记,以不同的激光剂量照射,采用图像分析系统（ＩＡＳ）和荧光显微镜观察线粒体跨膜电势荧光强度的变化。结果：低功率Ｈｅ－Ｎｅ激光照射５,１０,１５ｍｉｎ,激光剂量分别为０．６４９,１．３８８和２．０８２Ｊ／ｃｍ＾２,巨噬细胞线粒体跨膜电势荧光     

不同剂量的He—Ne激光对Raji细胞膜表面SH的影响

本文以离体培养的Ｒａｊｉ细胞为材料,采用ＥＬＬＭＡＮ方法检测了不同剂量的Ｈｅ－Ｎｅ激光对Ｒａｊｉ细胞膜表面ＳＨ含量的影响。发现０．５Ｊ／ｃｍ＾２Ｈｅ－Ｎｅ激光能量可明显增加膜表面ＳＨ含量（Ｐ〈０．０５）。大于或小于此剂量的Ｈｅ－Ｎｅ激光对膜表面ＳＨ含量的影响均不明显（Ｐ〉０．０５）。提示０．５Ｊ／ｃｍ＾２的激光能量对膜有刺激作用。     

He—Ne激光对机体免疫功能影响研究进展

本文仅就Ｈｅ－Ｎｅ激光照射对机体特异性与非特异性免疫功能的促进与抑制的研究现状作以综述。     

He－Ne激光对机体免疫功能影响研究进展

本文仅就Ｈｅ－Ｎｅ激光照射对机体特异性与非特异性免疫功能的促进与抑制的研究现状作以综述。     

He—Ne激光血管内照射血液治疗冠心病的临床研究

应用低强度Ｈｅ－Ｎｅ激光血管内照射（ＩＬＩＢ）疗法试治３２例冠心病患者,同时设正常对照２０例,进行血液流变学、血清ＭＤＡ水平及其它相关指标的疗前后对比检测。结果表明所有指标在ＩＬＩＢ治疗后比疗前有显著改善（Ｐ〈０．０１）,与病情好转一致。作者认为ＩＬＩＢ不失为冠心病治疗的有效手段之一,其疗效机理有待进一步研究。     

He－Ne激光照射糙米种胚对水稻当代生长发育的影响

Ｈｅ－Ｎｅ激光处理水稻去壳种子可以促进分蘖,提高成秧率和成穗率,增加有效穗数和每穗总粒数、千粒重及单株产量。但结实率降低、生育期延长。株高、穗长虽略有增加,但不明显。     

农用低功率He—Ne激光的诱变效应和育种

什么是低功率Ｈｅ－Ｎｅ激光;低功率Ｈｅ－Ｎｅ激光的诱变效应及其在育种上的应用;低功率Ｈｅ－Ｎｅ激光的诱变机制。     

YAG与He—Ne激光处理籼稻恢复系干种子的诱变育种效果的研究

用ＹＡＧ,Ｈｅ－Ｎｅ激光处理籼稻处理系恢４８－２干种子,统计了其当代其生物学效应,Ｌ２代的突变频率,几种主要性状的变异系数及广义遗传力,Ｌ２代各处理选１０个突变株与多个不良系配组,表现熟期较早,综合性状较好的组合数,ＹＡＧ激光处理的高于Ｈｅ－Ｎｅ处理的,并选育出了一个较好的新的恢复系－恢４１。     

He—Ne激光治疗Bell‘s面瘫的研究

本文报导了激光在治疗Ｂｅｌｌ＇ｓ面瘫中的应用。五例病人通过Ｈｅ－Ｎｅ激光穴位照射、低强血管内照射,和辅以外敷中药。经随访全部治愈。本治疗具有高疗效、不出血、操作简单等优点。该方法值得进一步研究和推广。     

He—Ne激光对小麦幼苗增强UV—B辐射损伤的修复效应

采用 He- Ne激光辐照对增强 UV- B辐射后小麦幼苗的损伤修复作用进行了研究。小麦种子在盛有湿滤纸的培养皿内 2 5℃下进行萌发。萌发后小麦幼苗在光合有效辐射 (PAR)为 2 2 0 μmol· m- 2 · S- 1的光背景下 ,经 1 0 .0 8k J·m- 2 · d- 1的增强 UV- B辐射 ,然后再用 5m W· mm- 2的 He- Ne激光进行辐照。通过小麦幼苗丙二醛 (MDA)、抗坏血酸 (As A)、超氧化物歧化酶 (SOD)和紫外吸收物含量及活性的变化 ,测定了 He- Ne激光对小麦 UV- B损伤修复的作用。结果显示 ,MDA、SOD、As A和紫外吸收物的变化同小麦幼苗损伤修复的能力相关联。He- Ne激光辐照可使 UV- B辐射后小麦幼苗 MDA的含量明显减少、As A含量明显增加、SOD活性增强及紫外吸收物含量显著增加。说明增强 UV- B对小麦幼苗生理水平的辐射损伤 ,能够被一定剂量的 He- Ne激光辐照而得到部分修复。但是 ,采用同等波长和功率的红光照射 ,其 MDA、SOD、As A和紫外吸收物均无明显变化 ,证明激光的促进修复作用并非由激光的光效应所致     

He-Ne激光辐照野桑蚕生物学效应研究

采用一定量He-Ne激光辐照野桑蚕催青卵及蚕肾,并与家蚕杂交,对其子代的蛋白质和酯酶同工酶进行电泳分析,结果发现谱带数目及活性有变化。另外,茧质调查和丝质调查与对照相比也有差异,并发现丝长和纤度特好的变异个体,显示He-Ne激光辐照对野桑蚕生理生化性状有一定影响。     

低强度激光对人红细胞生物物理特性的影响

研究不同功率的低强度He-Ne激光对正常人体红细胞流变学特性影响。以正常人体红细胞为研究对象,测量了低强度He-Ne激光在不同照射时间、不同功率条件下红细胞的变形、取向、膜流动性、膜的微粘度和渗透脆性的变化情况。结果表明:照射后红细胞的变形性和膜流动性增强、渗透脆性下降。照射对红细胞流变学特性影响显著,其中激光能量为0.24 J、照射血样为2 mL时取得的照射效果最佳。     

Interaction of the B subunit of cholera toxin with endogenous ganglioside GM1 causes changes in membrane potential of rat thymocytes

Summary The fluorescent anionic dye, bisoxonol, and flow cytometry have been used to monitor changes in the membrane potential of rat thymocytes exposed to the B subunit of cholera toxin. The B subunit induced a rapid hyperpolarization, which was due to activation of a Ca²⁺-sensitive K⁺ channel. Reduction of extracellular Ca²⁺ to <1 m by the addition of [ethylenebis(oxyethylenenitrilo)]tetraacetic acid immediately abolished the hyperpolarization caused by the B subunit. Cells treated with quinine and tetraethylammonium lost their ability to respond to the B subunit, whereas 4-aminopyridine did not have any effect. Thus, calcium-sensitive and not voltage-gated K⁺ channels appeared to be responsible for the hyperpolarization. The results of ion substitution experiments indicated that extracellular Na⁺ was not essential for changes in membrane potential. Further studies with ouabain, amiloride and furosemide demonstrated that electrogenic Na⁺/K⁺ ATPase, Na⁺/H⁺ antiporter and Na⁺/K⁺/Cl^– cotransporter, respectively, were not involved in the hyperpolarization process induced by the B subunit. Thus, crosslinking of several molecules of ganglioside GM1 on the cell surface of rat thymocytes by the pentavalent B subunit of cholera toxin modulated plasma membrane permeability to K⁺ by triggering the opening of Ca²⁺-sensitive K⁺ channels. A role for gangliosides in regulating ion permeability would have important implications for the function of gangliosides in various cellular phenomena.     

He-Ne激光和增强UV-B辐射对小麦幼苗根抗氧化酶的影响

采用5 mW·mm-2He-Ne激光辐照、10.08 kJ·m-2d-1 UV-B辐射及二者组合对冬小麦“临优2018”幼苗进行处理,研究各处理组幼苗根过氧化物酶(POD)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性及同工酶变化.研究结果显示,增强UV-B辐射能抑制POD、CAT和SOD的活性,差异显著,关闭了...     

烟草花粉母细胞细胞融合过程中细胞骨架的超微结构观察

应用普通电镜和DGD去包埋技术 ,研究了烟草花粉母细胞中的细胞融合现象及细胞融合过程中细胞骨架的变化。观察发现 ,处于凝线期的花粉母细胞 ,其内含物 ,包括细胞器和染色质 ,主要通过胞质通道向相邻细胞发生转移。DGD去包埋观察发现 ,花粉母细胞中核骨架与细胞质内及胞间连丝和胞质通道内胞质骨架连接成一个整体。在整个细胞融合过程中 ,均有核骨架纤维与染色质相连。本文讨论了细胞骨架在细胞融合过程中的作用     

低强度He-Ne激光照射人外周血淋巴细胞两组试验结果的分析

本文采用染色体畸变 (chromosomalaberration CA)试验和微核 (micronucleus)试验两种方法对低强度He Ne激光辐照育龄妇女外周血淋巴细胞。激光能量密度分别为 14.31J cm2 (辐照 5′)、2 8.6 2J cm2 (辐照 10′) ,5 7.2 4J cm2(辐照 2 0′) ,114.5 2J cm2 (辐照 40′)。照射血样后 ,染色体畸变试验检测其淋巴细胞染色体畸变率 ,激光照射及空白对照组 ,血样染色体畸变率分别为 4.2 9‰、3.96‰、3.81、3.5 9‰和 4.19‰ ,X2 检验无显著意义 (P >0 .0 5 )。阳性对照丝裂霉素MMC处理的血样淋巴细胞CA率平均为 14.41‰ ,明显高于激光照射和空白对照组 ,X2 检验有显著差异(P <0 .0 1)。微核试验检测结果 ,微核染色体分别为 1.0 2‰ ,1.17‰ ,1.18‰ ,1.31‰和 1.19‰对照 ,经统计分析激光照射各组与对照组微核率均在 2‰以下 (P >0 .0 5 ) ,属正常人体微核范围内。结果显示两组试验监测诱变均具有一致性。证明He Ne激光辐照人体细胞对染色体无致畸效应。且表明He Ne激光在治疗范围内应用安全、有效、不会对不体造成危害。     

IAA-induced hyperpolarization of the membrane potential in isolated cells fromMimosa pulvinus

Upon treatment with 10⁻⁴ M IAA the membrane potential of an isolated cell from the main pulvinus, ofMimosa pudica L. depolarized by about 6 mV in 2–5 min, but later it gradually hyperpolarized by about 30 mV. The membrane potential of a motor cell in the main pulvinar tissue hyperpolarized by about 80 mV 1 hr after application of 10⁻⁴ M IAA.     

Effect of high pH on the plasma membrane potential and conductance in Elodea densa

In leaves of Elodea densa the membrane potential measured in light equals the equilibrium potential of H+ on the morphological upper plasma membrane. The apoplastic pH on the upper side of the leaf is as high as 10.5-11.0, which indicates that alkaline pH induces an increased H+ permeability of the plasmalemma. To study this hypothesis in more detail we investigated the changes in membrane potential and conductance in response to alterations in the external pH from 7 (= control) to 9 or 11 under both light and dark conditions. Departing from the control pH 7 condition, in light and in dark the application of pH 9 resulted in a depolarization of the membrane potential to the Nernst potential of H+. In the light but not in the dark, this depolarization was followed by a repolarization to about -160 mV. The change to pH 9 induced, in light as well as in dark, an increase in membrane conductance. The application of pH 11, which caused a momentary hyper- or depolarization depending on the value at the time pH 11 was applied, brought the membrane potential to around -160 mV. The membrane conductance also increased, in comparison to its value at pH 7, as a result of the application of pH 11, irrespective of the light conditions.     

Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture

Neuroprogenitor cells (NPCs) isolated from the human fetal brain were expanded under proliferative conditions in the presence of epidermal growth factor (EGF) and fibroblast growth factor (FGF) to provide an abundant supply of cells. NPCs were differentiated in the presence of a new combination of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), dibutyryl cAMP (DBC) and retinoic acid on dishes coated with poly-L-lysine and mouse laminin to obtain neuron-rich cultures. NPCs were also differentiated in the absence of neurotrophins, DBC and retinoic acid and in the presence of ciliary neurotrophic factor (CNTF) to yield astrocyte-rich cultures. Differentiated NPCs were characterized by immunofluorescence staining for a panel of neuronal markers including NeuN, synapsin, acetylcholinesterase, synaptophysin and GAP43. Glial fibrillary acidic protein (GFAP) and STAT3, astrocyte markers, were detected in 10-15% of differentiated NPCs. To facilitate cell-type specific molecular characterization, laser capture microdissection was performed to isolate neurons cultured on polyethylene naphthalate (PEN) membrane slides. The methods described in this study provide valuable tools to advance our understanding of the molecular mechanism of neurodegeneration.