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1.
AtT20 (clone D16V) cells develop long neurite-like processes in the growth cones of which secretory granules containing ACTH accumulate. These secretory granules have an acidic pH. Using acridine orange as a vital stain for acidic organelles, in combination with video-enhanced fluorescence microscopy, and subsequent immunolabeling with rabbit antibodies against ACTH, we have shown that these secretory granules move by saltations along the processes. During saltations velocities of 3 to 5 microns/s are achieved. The majority of the secretory granules move in the anterograde direction but some move retrogradely. The growth cones and processes are the site of extensive endocytosis. Using Lucifer Yellow as a vital stain we have shown that most endosomes move by saltations retrogradely. Movement of both secretory granules and endosomes is dependent upon microtubules. Individual secretory granules or endosomes never reverse the direction of their movement as they traverse the processes. Neutralization of the lumen of these acidic organelles with NH4Cl does not inhibit their movement or change its direction.  相似文献   

2.
Van Haastert PJ 《PloS one》2011,6(11):e27532
Amoeboid cells crawl using pseudopods, which are convex extensions of the cell surface. In many laboratory experiments, cells move on a smooth substrate, but in the wild cells may experience obstacles of other cells or dead material, or may even move in liquid. To understand how cells cope with heterogeneous environments we have investigated the pseudopod life cycle of wild type and mutant cells moving on a substrate and when suspended in liquid. We show that the same pseudopod cycle can provide three types of movement that we address as walking, gliding and swimming. In walking, the extending pseudopod will adhere firmly to the substrate, which allows cells to generate forces to bypass obstacles. Mutant cells with compromised adhesion can move much faster than wild type cells on a smooth substrate (gliding), but cannot move effectively against obstacles that provide resistance. In a liquid, when swimming, the extending pseudopods convert to side-bumps that move rapidly to the rear of the cells. Calculations suggest that these bumps provide sufficient drag force to mediate the observed forward swimming of the cell.  相似文献   

3.
Campbell RD 《Tissue & cell》1990,22(2):137-147
The histological pathway by which intracellular symbiotic Chlorella move into the developing oocytes of hydra was investigated at the ultrastructural level. Algae move from within the digestive cells of the endoderm to within the oocytes of the ectoderm in a three-step process. First, the algae are released by digestive cells into the mesolamella (basement membrane). Second, the algae move as individual cells into the adjacent intercellular spaces of the ectoderm. Third, they are taken up by the oocyte by phagocytosis. This transfer occurs only in the central regions of the ovary, and only after oocytes have reached an advanced stage. Normally the mesolamella is separated from the ectodermal interstitial spaces by a layer of epitheliomuscular cell muscle processes. This layer degenerates in the region where algae will move into the ectoderm. This study shows that algae move as individual cells and not intracellularly within processes of the epithelial cells.  相似文献   

4.
Bonner JT 《Mycologia》2006,98(2):260-264
By comparing two species of cellular slime molds that have stalkless migration stages it is possible to gain interesting insights into how the cells move. In contrast to the familiar behavior of Dictyostelium discoideum, Dictyostelium polycephalum slugs can travel greater distances through soil and even can migrate through agar. In addition to the interest in the differences, these differences shed light on the mechanism of slug movement. Unlike D. discoideum, D. polycephalum does not have prestalk and prespore zones and severed sections of any part of these slugs move at a rate proportional to their length. This leads to the hypothesis that longer slugs move faster because the amoebae aligned along the inside of the slime sheath each contribute a forward push and the more extended the amoebae line is the faster the slug moves.  相似文献   

5.
6.
Phospholipids have been treated as dimers on a hexagonal lattice, and a move has been introduced that allows the dimers to move and change their orientation on the lattice. Simulations have been performed in which phospholipid chains have been treated as being either independent or infinitely coupled thermodynamically with regard to their conformational state. Both types of simulation have reproduced well experimental heat-capacity curves of dipalmitoyl phosphatidylcholine small unilamellar vesicles. Apart from a different gel-fluid interaction parameter and a different number of unlike nearest-neighbor contacts, most of the averages and thermodynamic quantities were essentially the same in the two types of simulation. These results indicate that the transition is not first order and validate those of previous Monte Carlo simulations that have neglected the dimeric nature of phospholipids in the sense that they show that for the thermotropic transition the approximation of phospholipids as monomers is valid.  相似文献   

7.
The mechanisms that control spindle structure and move chromosomes remain as mysterious as ever. Using the diatom as a model spindle type, several interesting and counter-intuitive possibilities have emerged. For example, there may not be an anaphase motor and a primary energy-requiring (force-producing) mechanism may actually move chromosomes away from the pole. Force may be a primary factor controlling microtubule assembly disassembly.  相似文献   

8.
Melanosomes on the move: a model to understand organelle dynamics   总被引:1,自引:0,他引:1  
Advances in live-cell microscopy have revealed the extraordinarily dynamic nature of intracellular organelles. Moreover, movement appears to be critical in establishing and maintaining intracellular organization and organellar and cellular function. Motility is regulated by the activity of organelle-associated motor proteins, kinesins, dyneins and myosins, which move cargo along polar MT (microtubule) and actin tracks. However, in most instances, the motors that move specific organelles remain mysterious. Over recent years, pigment granules, or melanosomes, within pigment cells have provided an excellent model for understanding the molecular mechanisms by which motor proteins associate with and move intracellular organelles. In the present paper, we discuss recent discoveries that shed light on the mechanisms of melanosome transport and highlight future prospects for the use of pigment cells in unravelling general molecular mechanisms of intracellular transport.  相似文献   

9.
In a wide range of problem-solving settings, the presence of a familiar solution can block the discovery of better solutions (i.e., the Einstellung effect). To investigate this effect, we monitored the eye movements of expert and novice chess players while they solved chess problems that contained a familiar move (i.e., the Einstellung move), as well as an optimal move that was located in a different region of the board. When the Einstellung move was an advantageous (but suboptimal) move, both the expert and novice chess players who chose the Einstellung move continued to look at this move throughout the trial, whereas the subset of expert players who chose the optimal move were able to gradually disengage their attention from the Einstellung move. However, when the Einstellung move was a blunder, all of the experts and the majority of the novices were able to avoid selecting the Einstellung move, and both the experts and novices gradually disengaged their attention from the Einstellung move. These findings shed light on the boundary conditions of the Einstellung effect, and provide convergent evidence for Bilalić, McLeod, & Gobet (2008)’s conclusion that the Einstellung effect operates by biasing attention towards problem features that are associated with the familiar solution rather than the optimal solution.  相似文献   

10.
Chacma baboons (Papio ursinus) intentionally overturn rocks to feed on the invertebrates beneath. However, baboons do not move all the rocks they encounter, with this presumably reflecting cost–benefit (or effort–reward) trade‐offs in their foraging behavior. We ask, how do “clever baboons” choose rock sizes and shapes and move these rocks? Using optimal foraging theory, we predicted that baboons would prefer to move medium‐sized rocks, a trade‐off between moving larger rocks that might require more effort to move, and smaller rocks that likely do not provide enough prey (the reward) to make the effort worthwhile. We also expected baboons to prefer rounded rocks as these will require less energy to move by rolling (rather than being flipped as for flat rocks) and that the effort of rock movement might be offset by moving rocks along the shortest axis. We show that baboons have clear preferences for specific rock sizes (medium‐sized) and shapes (angular and flat when these were medium‐sized), and the way in which rocks are moved (along the shortest axis). Prey occurred infrequently under rocks. The low predictability of prey beneath rocks suggests that such prey, when encountered, is of considerable value to baboons for them to expend the search effort, and also explains the extensive nature of rock movement by baboons in the landscape. Our study provides a novel application of the optimal foraging theory concept and has important implications for understanding and predicting how animals choose to move rocks.  相似文献   

11.
G Laevsky  D A Knecht 《BioTechniques》2001,31(5):1140-2, 1144, 1146-9
Under-agarose chemotaxis has been used previously to assess the ability of neutrophils to respond to gradients of chemoattractant. We have adapted this assay to the chemotactic movement of Dictyostelium amoebae in response to folic acid. Troughs are used instead of wells to increase the area along which the cells can be visualized and to create a uniform front of moving cells. Imaging the transition zone where the cells first encounter the agarose, we find that the cells move perpendicular to the gradient and periodically manage to squeeze under the agarose and move up the gradient. As cells exit the troughs, their cross-sectional area increases as the cells become flattened. Three-dimensional reconstruction of confocal optical sections through GFP-labeled cells demonstrates that the increase in cross-sectional area is due to the flattening of the cells. Since the cells locally deform the agarose and become deformed by it, the concentration of the agarose, and therefore its stiffness, should affect the ability of the cells to migrate. Consistent with this hypothesis, cells in 0.5% agarose move faster and are less flat than cells under 2% agarose. Cells do not exit the troughs and move under 3% agarose at all. Therefore, this assay can be used to compare and quantify the ability of different cell types or mutant cell lines to move in a restrictive environment.  相似文献   

12.
The presented approach focuses on the attempt to specify strategies of visually organizing sequences of different hand movements and the resulting fine-control of movement close to the target, since in skilled activities our ability to sequence a number of separate movements each having different spatiotemporal characteristics is of central importance. Sequences of different moves have been analysed here. After a gross distance covering part of the move, small correction movements are performed close to the target to reduce the position error. The length of the required correction movements and the corresponding positioning time increase with target distance. In order to investigate motor control strategies two different tasks, with and without time pressure, have been designed. Time pressure forces the subjects to finish the previous move and to prepare the next move simultaneously. Absolute, constant and variable errors revealed that under time pressure the subject changes the control strategy by increasing the constant and reducing the variable error.  相似文献   

13.
In euechinoid sea urchin embryos, a subset of epithelial cells in the wall of the blastula become pulsatile, elongate, lose connections with their neighboring cells, and move into the blastocoel to form the primary mesenchyme cells. The Golgi apparatus and microtubule organizing center (MTOC) are located at the apical end of these epithelial cells. We show that as primary mesenchyme cells begin to move into the blastocoel, the Golgi apparatus and MTOC move to a new position adjacent to the apical side of the nucleus. They do not move to a position between the nucleus and the leading (i.e., basal) end of the cell as they do in cultured fibroblasts undergoing directed migration. In addition, we have inhibited the movement of membranous vesicles to the cell surface by incubating embryos in the ionophore monensin. We have used antibodies to msp130, a primary mesenchyme cell surface-specific glycoprotein, to demonstrate that monensin inhibits the movement of msp130-containing vesicles to the cell surface. Despite the inhibition of membrane shuttling by monensin, primary mesenchyme cells ingress on schedule and display normal cell-shape changes. We draw two conclusions from our data. First, the cellular elongation that characterizes ingression is not due to the local insertion of membrane at the leading (basal) end of the cell. Second, ingression does not depend upon establishment of the same cell polarity required for fibroblasts to carry out directed cell migration.  相似文献   

14.
This paper investigates several strategies for prey and predator in both bounded and unbounded domains, assuming they have the same speed. The work describes how the prey should move to escape from the predator and how predator should move to catch the prey. The approach is agent-based and explicitly tracks movement of individuals as prey and predator. We show that the prey escapes one or two competing predators, while might be caught in the case of three predators. The paper also describes a strategy for finding a well camouflaged static prey which emits signals.  相似文献   

15.
Motility is often a virulence factor of pathogenic bacteria. Although recent works have identified genes involved in gliding motility of mycoplasmas, little is known about the mechanisms governing the cell gliding behaviour. Here, we report that Mycoplasma genitalium MG217 is a novel protein involved in the gliding apparatus of this organism and it is, at least, one of the genes that are directing cells to move in narrow circles when they glide. In the absence of MG_217 gene, cells are still able to glide but they mainly move drawing erratic or wide circular paths. This change in the gliding behaviour correlates with a rearrangement in the terminal organelle disposition, suggesting that the terminal organelle operates as a guide to steer the mycoplasma cell in a specific direction. Immunogold labelling reveals that MG217 protein is located intracellular at the distal end of the terminal organelle, between the cell membrane and the terminal button. Such location is consistent with the idea that MG217 could act as a modulator of the terminal organelle curvature, allowing cells to move in specific directions.  相似文献   

16.
Recent experiments have revealed an area of visual cortex that provides a velocity error signal which enables the eye to learn to pursue targets when they move in a predictable way.  相似文献   

17.
Communication between cells is crucial for proper functioning of multicellular organisms. The recently discovered membranous tubes, named tunneling nanotubes, that directly bridge neighboring cells may offer a very specific and effective way of intercellular communication. Our experiments on RT4 and T24 urothelial cell lines show that nanotubes that bridge neighboring cells can be divided into two types. The nanotubes of type I are shorter and more dynamic than those of type II, and they contain actin filaments. They are formed when cells explore their surroundings to make contact with another cell. The nanotubes of type II are longer and more stable than type I, and they have cytokeratin filaments. They are formed when two already connected cells start to move apart. On the nanotubes of both types, small vesicles were found as an integral part of the nanotubes (that is, dilatations of the nanotubes). The dilatations of type II nanotubes do not move along the nanotubes, whereas the nanotubes of type I frequently have dilatations (gondolas) that move along the nanotubes in both directions. A possible model of formation and mechanical stability of nanotubes that bridge two neighboring cells is discussed.  相似文献   

18.
Crowd evacuations are paradigmatic examples for collective behaviour, as interactions between individuals lead to the overall movement dynamics. Approaches assuming that all individuals interact in the same way have significantly improved our understanding of pedestrian crowd evacuations. However, this scenario is unlikely, as many pedestrians move in social groups that are based on friendship or kinship. We test how the presence of social groups affects the egress time of individuals and crowds in a representative crowd evacuation experiment. Our results suggest that the presence of social groups increases egress times and that this is largely due to differences at two stages of evacuations. First, individuals in social groups take longer to show a movement response at the start of evacuations, and, second, they take longer to move into the vicinity of the exits once they have started to move towards them. Surprisingly, there are no discernible time differences between the movement of independent individuals and individuals in groups directly in front of the exits. We explain these results and discuss their implications. Our findings elucidate behavioural differences between independent individuals and social groups in evacuations. Such insights are crucial for the control of crowd evacuations and for planning mass events.  相似文献   

19.
ISWI proteins form the catalytic core of a subset of ATP-dependent chromatin remodeling activities in eukaryotes from yeast to man. Many of these complexes have been found to reposition nucleosomes but with different directionalities. We find that the yeast Isw1a, Isw2, and Chd1 enzymes preferentially move nucleosomes toward more central locations on short DNA fragments whereas Isw1b does not. Importantly, the inherent positioning properties of the DNA play an important role in determining where nucleosomes are relocated to by all of these enzymes. However, a key difference is that the Isw1a, Isw2, and Chd1 enzymes are unable to move nucleosomes to positions closer than 15 bp from a DNA end, whereas Isw1b can. We also find that there is a correlation between the inability of enzymes to move nucleosomes close to DNA ends and the preferential binding to nucleosomes bearing linker DNA. These observations suggest that the accessibility of linker DNA together with the positioning properties of the underlying DNA play important roles in determining the outcome of remodeling by these enzymes.  相似文献   

20.
We have examined the rates of chromosome and pole motion during anaphase in HeLa cells using differential interference contrast and polarization optics. In early anaphase both chromosomes and poles move apart. When the chromosomes are separated by a distance about equal to the metaphase spindle length, both chromosomes and poles slow but continue to move at a reduced rate. Throughout anaphase, the chromosomes move faster than the poles, so the chromosome-to-pole distance decreases. Treatment of the cells with about 5 × 10?8 M colchicine up to 45 min before observation tends to block normal formation of metaphase spindles, but more than half of the cells in metaphase go on through anaphase. In these cells, both chromosome and pole motions are essentially normal until the chromosomes are separated by a distance equal to the length of the metaphase spindle. After that time, chromosome motion is supressed and the poles move slowly toward one another. These data suggest that the mechanism of anaphase motion changes character when the chromosomes become spaced by the metaphase spindle length. We call anaphase before and after that time phase 1 and phase 2, respectively. The results are discussed in the light of a sliding tubule model for chromosome motion.  相似文献   

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