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1.
Comparison of rat mesenchymal stem cells derived from bone marrow,synovium, periosteum,adipose tissue,and muscle 总被引:18,自引:0,他引:18
Yoshimura H Muneta T Nimura A Yokoyama A Koga H Sekiya I 《Cell and tissue research》2007,327(3):449-462
Mesenchymal stem cells (MSCs) are increasingly being reported as occurring in a variety of tissues. Although MSCs from human
bone marrow are relatively easy to harvest, the isolation of rodent MSCs is more difficult, thereby limiting the number of
experiments in vivo. To determine a suitable cell source, we isolated rat MSCs from bone marrow, synovium, periosteum, adipose,
and muscle and compared their properties for yield, expansion, and multipotentiality. After two passages, the cells in each
population were CD11b (−), CD45 (−), and CD90 (+). The colony number per nucleated cells derived from synovium was 100-fold
higher than that for cells derived from bone marrow. With regard to expansion potential, synovium-derived cells were the highest
in colony-forming efficiency, fold increase, and growth kinetics. An in vitro chondrogenesis assay demonstrated that the pellets
derived from synovium were heavier, because of their greater production of cartilage matrix, than those from other tissues,
indicating their superiority in chondrogenesis. Synovium-derived cells retained their chondrogenic potential after a few passages.
The Oil Red-O positive colony-rate assay demonstrated higher adipogenic potential in synovium- and adipose-derived cells.
Alkaline phosphatase activity was greater in periosteum- and muscle-derived cells during calcification. The yield and proliferation
potential of rat MSCs from solid tissues was much better than those from bone marrow. In particular, synovium-derived cells
had the greatest potential for both proliferation and chondrogenesis, indicating their usefulness for cartilage study in a
rat model.
This study was supported in part by grants from the Japan Latest Osteoarthritis Society and from the Center of Excellence
Program for Frontier Research on Molecular Destruction and Reconstruction of Tooth and Bone in Tokyo Medical and Dental University
(to T.M.), and by the Japan Society for the Promotion of Science (grant no. 18591657 to I.S.). Recombinant human bone morphogenetic
protein-2 was kindly provided by Astellas Pharma. 相似文献
2.
Tendon-bone healing is important for the successful reconstruction of the anterior cruciate ligament by using the hamstring
tendon. Mesenchymal stem cells (MSCs) have attracted much interest because of their self-renewing potential and multipotentiality
for possible clinical use. We previously reported that MSCs derived from synovium had a higher proliferation and differentiation
potential than the other MSCs that we examined. The purpose of this study was to investigate the effect and mechanism of the
implantation of the synovial MSCs on tendon-bone healing in rats. Half of the Achilles’ tendon grafts of rats were inserted
into a bone tunnel from the tibial plateau to the tibial tuberosity with a suture-post fixation. The bone tunnel was filled
with MSCs labeled with fluorescent marker DiI or without MSCs as the control. The tendon-bone interface was analyzed histologically,
and collagen fibers were quantified. At 1 week, the tendon-bone interface was filled with abundant DiI-positive cells, and
the proportion of collagen fiber area was significantly higher in the MSC group than in the control group. By 2 weeks, the
proportion of oblique collagen fibers, which appeared to be Sharpey’s fibers, was significantly higher in the MSC group than
in the control group. At 4 weeks, the interface tissue disappeared, and the implanted tendon appeared to attach to the bone
directly in both groups. DiI-labeled cells could no longer be observed. Implantation of synovial MSCs into bone tunnel thus
accelerated early remodeling of tendon-bone healing, as shown histologically.
This study was supported in part by grants from the Japan Society for the Promotion of Science (19591752) and from the Center
of Excellence Program for Frontier Research on Molecular Destruction and Reconstruction of Tooth and Bone at Tokyo Medical
and Dental University to T.M. and from the Japan Society for the Promotion of Science (18591657) to I.S. 相似文献
3.
In vitro chondrogenesis of human synovium-derived mesenchymal stem cells: optimal condition and comparison with bone marrow-derived cells 总被引:12,自引:0,他引:12
Shirasawa S Sekiya I Sakaguchi Y Yagishita K Ichinose S Muneta T 《Journal of cellular biochemistry》2006,97(1):84-97
There are increasing reports that mesenchymal stem cells (MSCs) are present in various tissues other than bone marrow, including synovium. Here we investigated the optimal conditions for in vitro chondrogenesis of human synovium-derived MSCs and compared these cells with bone marrow-derived MSCs, especially in terms of their chondrogenesis potential. Synovium and bone marrow were harvested from six donors during knee operations for ligament injuries. Digested synovium cells or nucleated cells from bone marrow were expanded clonally. A pellet culture system was used for chondrogenesis, and the best combination of up to three cytokines of the seven assessed. Synovium-derived MSCs plated at a lower density expanded more rapidly. Contrary to previous reports, a combination of TGFbeta and dexamethasone was not sufficient to induce chondrogenesis. However, addition of BMP2 to TGFbeta and dexamethasone dramatically increased cartilage pellet size and the synthesis of cartilage matrix. The cartilage pellets were also analyzed by electron microscopy and immunohistology. DNA content per pellet decreased during chondrogenesis, indicating the pellet increased its size through the accumulation of newly synthesized extracellular matrix. Sequential chondrogenic gene expression was demonstrated by RT-PCR. Synovium-derived MSCs looked similar to the bone marrow-derived MSCs in their surface epitopes and proliferation potential; however, cartilage pellets from synovium were significantly larger than those from bone marrow in patient-matched comparisons. We demonstrated that the combination of TGFbeta, dexamethasone, and BMP2 was optimal for in vitro chondrogenesis of synovium-derived MSCs and that the synovium-derived MSCs have a greater chondrogenesis potential than bone marrow-derived MSCs. 相似文献
4.
Takaki Shimada Fumio Nakamura 《In vitro cellular & developmental biology. Plant》1988,24(12):1193-1200
Summary A certain kind of cell in the pituitary gland exhibited immunoreactive keratin and dome formations in vitro. We obtained epithelial
cells, which were able to subculture, from the outgrowth of anterior pituitary organ cultures. These cells lacked hormone
secretory granules and exhibited immunoreactive keratin. Furthermore, they produced dome formations or cystic structures in
monolayer culture and under three-dimensional culture condition using type I collagen gel. Dome formation was stimulated by
dibutyryl cyclic AMP (dbcAMP, 10−3 to 10−5
M). Their responsiveness to dbcAMP is similar to that of several other epithelial cells that possess transport functions in
vivo and in vitro. Although the origin of our cultured cells is unknown, these cells formed dome formations that possessed
transport function and were related to cystic structures in the pituitary gland in vivo.
The study was supported by Grants in Aid for Scientific Research 60570018, 60870002 (for Dr. H. Ishikawa), and by The Science
Research Promotion Fund from Japan Private School Promotion Foundation (for Dr. H. Ishikawa). 相似文献
5.
Cartilage has a limited self-repair capability and the repair of large cartilage defects remains a challenge in clinic. This
study aimed to investigate the effect of mesenchymal stem cells (MSCs) loaded three-dimensional bilayer collagen scaffold
for cartilage repair. Cross-linked three-dimensional bilayer collagen scaffolds seeded with or without MSCs were implanted
into large cartilage defects (4 mm in diameter; 3 mm in depth) in rabbit knees. The untreated cartilage defects served as
control. The tissue response was evaluated at 6 and 12 weeks after implantation by general histology and semi-quantitative
histological grading systems. In addition, the repaired tissues were evaluated by mechanical test at 12 weeks after implantation.
The MSCs-loaded collagen scaffold group showed the most hyaline cartilage, highest histological scores and compressive modulus.
Moreover, it showed a good integration with the subchondral bone and adjacent cartilage. The structure of the novel bilayer
collagen scaffolds provided architectural support for the differentiation of MSCs and demonstrated successful induction of
in vivo chondrogenesis. These findings suggested that MSCs-loaded bilayer collagen scaffold could be an appealing candidate
to be used for cartilage regeneration. 相似文献
6.
In vivo chondrogenesis of adult bone-marrow-derived autologous mesenchymal stem cells 总被引:10,自引:0,他引:10
Chen J Wang C Lü S Wu J Guo X Duan C Dong L Song Y Zhang J Jing D Wu L Ding J Li D 《Cell and tissue research》2005,319(3):429-438
The purpose of this study has been to investigate the possible effects of the normal joint cavity environment on chondrocytic differentiation of bone-marrow-derived mesenchymal stem cells (MSCs). Autologous bone marrow was aspirated from the iliac crest of male sheep. MSCs were purified, expanded, and labeled with the fluorescent dye PKH26. Labeled MSCs were then grown on a three-dimensional porous scaffold of poly (L-lactic-co-glycolic acid) in vitro and implanted into the joint cavity by a surgical procedure. At 4 or 8 weeks after implantation, the implants were removed for histochemical and immunohistochemical analysis. The cells labeled with red fluorescent PKH26 in the implants expressed type II collagen and synthesized sulfated proteoglycans. However, the osteoblast-specific marker, osteocalcin, was not detected by immunohistochemistry indicating that the implanted MSCs had not differentiated into osteoblasts by being directly exposed to the normal joint cavity. To investigate the possible factors involved in chondrocytic differentiation of MSCs further, we co-cultured sheep MSCs with the main components of the normal joint cavity, viz., synovial fluid or synovial cells, in vitro. After 1 or 2 weeks of co-culture, the MSCs in both co-culture systems expressed markers of chondrogenesis. These results suggest that synovial fluid and synovium from normal joint cavity are important for the chondrocytic differentiation of adult bone-marrow-derived MSCs.This work was supported by the National Natural Science Foundation of China (nos. 39900036, 20174006, and 20221402), the National Advanced Technology Programs of China (nos. 2003AA744051, 2003AA205041), the Award Foundation for Young Teachers from the Ministry of Education, 973 project (no. G1999054306-03), and the 248 key innovative project of Beijing (no. H010210190123). 相似文献
7.
Koga H Muneta T Nagase T Nimura A Ju YJ Mochizuki T Sekiya I 《Cell and tissue research》2008,333(2):207-215
We previously compared mesenchymal stem cells (MSCs) from a variety of mesenchymal tissues and demonstrated that synovium-MSCs had the best expansion and chondrogenic ability in vitro in humans and rats. In this study, we compared the in vivo chondrogenic potential of rabbit MSCs. We also examined other parameters to clarify suitable conditions for in vitro and in vivo cartilage formation. MSCs were isolated from bone marrow, synovium, adipose tissue, and muscle of adult rabbits. Proliferation potential and in vitro chondrogenic potential were compared. Toxicity of the tracer DiI for in vitro chondrogenesis was also examined. MSCs from each tissue were embedded in collagen gel and transplanted into full thickness cartilage defects of rabbits. Cartilage matrix production was compared histologically. The effects of cell density and periosteal patch on the in vivo chondrogenic potential of synovium-MSCs were also examined. Synovium- and muscle-MSCs had a higher proliferation potential than other cells. Pellets from synovium- and bone-marrow-MSCs showed abundant cartilage matrix. DiI had no significant influence on in vitro cartilage formation. After transplantation into cartilage defects, synovium- and bone-marrow-MSCs produced much more cartilage matrix than other cells. When synovium-MSCs were transplanted at a higher cell density and with a periosteal patch, more abundant cartilage matrix was observed. Thus, synovium- and bone-marrow-MSCs had greater in vivo chondrogenic potential than adipose- and muscle-MSCs, but synovium-MSCs had the advantage of a greater proliferation potential. Higher cell density and a periosteum patch were needed to obtain a high production of cartilage matrix by synovium-MSCs. 相似文献
8.
Constructs containing autogenous mesenchymal stem cells (MSCs) seeded in collagen gels have been used by our group to repair rabbit central patellar tendon defect injuries. Although these cell-gel composites exhibit improved repair biomechanics compared to natural healing, they can be difficult to handle at surgery and lack the necessary stiffness to resist peak in vivo forces early thereafter. MSCs are typically suspended in collagen gels around two posts in the base of a well in a specially designed silicone dish. The distance between posts is approximately the length of the tendon wound site. MSCs contract the gel around the posts prior to removal of the construct for implantation at surgery. We hypothesized that in vitro construct alignment and stiffness might be enhanced in the midregion of the longer construct where the end effects of the posts on the bulk material (St. Venant effects) could be minimized. Rabbit MSCs were seeded in purified bovine collagen gel at 0.04 M cells/mg collagen. The cell-gel mixture was pipetted into silicone dishes having two post-to-post lengths (short: 11 mm and long: 51 mm) but equivalent well widths and depths and post diameters. After 14 days of incubation, tensile stiffness and modulus of the constructs were measured using equivalent grip-to-grip lengths. Collagen fiber orientation index or OI (which measures angular dispersion of fibers) was quantified using small angle light scattering (SALS). Long constructs showed significantly lower angular dispersion vs. short constructs (OI of 41.24 degrees +/-1.57 degrees vs. 48.43 degrees +/-1.27 degrees , mean+/-SEM, p<0.001) with significantly higher linear modulus (0.064+/-0.009 MPa vs. 0.024+/-0.004 MPa, p=0.0022) and linear stiffness (0.031+/-0.005 MPa vs. 0.018+/-0.004 N/mm, mean+/-SEM, respectively, p=0.0404). We now plan to use principles of functional tissue engineering to determine if repairs containing central regions of longer MSC-collagen constructs improve defect repair biomechanics after implantation at surgery. 相似文献
9.
Yokoyama A Muneta T Nimura A Koga H Mochizuki T Hata Y Sekiya I 《Cell and tissue research》2007,329(3):469-478
Elastic cartilage-derived cells cultured two-dimensionally with FGF2 and corticosteroid produce gel-type masses that become
mature cartilage when injected into a subcutaneous pocket. This unique method has previously been clinically applied for treatments
of nasal augmentation. However, the components of the gel-type mass and the mechanism of its synthesis remain unknown. Here,
we have investigated the components of the gel-type mass produced by elastic cartilage-derived cells, and whether this gel-type
mass can be produced by using other cell sources or other media. Human elastic cartilage-derived cells from auricular cartilage,
hyaline cartilage-derived cells from articular cartilage, and mesenchymal stem cells from synovium were cultured in three
media: “redifferentiation medium” containing FGF2 and dexamethasone; “chondrogenic medium” containing bone morphogenetic protein-2,
transforming growth factor-β3, and dexamethasone specific for in vitro chondrogenesis of mesenchymal stem cells; control medium.
The elastic cartilage-derived cells cultured in redifferentiation medium produced a gelatinous matrix positive for Alcian
blue. During culture, the amount of chondroitin 4-sulfate, chondroitin 6-sulfate, and especially hyaluronan increased. However,
the expression of RNAs for most chondrogenic genes did not increase. We also reproduced cartilage tissue formation by the
injection of elastic cartilage-derived cells with the gelatinous mass into the subcutaneous space of the nude mouse. The synthesis
of gelatinous matrix in vitro and the formation of cartilage tissue in vivo could be obtained only for the combination of
elastic cartilage-derived cells with redifferentiation medium.
This study was supported in part by grants from the “Japan Society for the Promotion of Science (19591752)” and “Center of
Excellence Program for Frontier Research on Molecular Destruction and Reconstruction of Tooth and Bone in Tokyo Medical and
Dental University” to Takeshi Muneta, and the “Japan Society for the Promotion of Science (18591657)” to Ichiro Sekiya. 相似文献
10.
Teruo Nishida Atsuko Ueda Masahiko Fukuda Hiroshi Mishima Kyoko Yasumoto Toshifumi Otori 《In vitro cellular & developmental biology. Plant》1988,24(10):1009-1014
Summary Corneal fibroblasts, also known as keratocytes are surrounded by an extracellular matrix of collagen in vivo. To understand
the physiology and pathology of these corneal fibroblasts, it is important to study their interactions with this extracellular
matrix. We cultured rabbit corneal fibroblasts on tissue culture plastic dishes or in a hydrated collagen gel and compared
the changes in morphology and mitotic activity. Corneal fibroblasts on plastic dishes were flattened and widely spread, whereas
those in collagen gel became spindle-shaped with long processes. Examination with an electron microscope revealed that the
corneal fibroblasts in collagen gel formed gap junctions with neighboring cells. Gap junctions were hardly ever observed between
corneal fibroblasts cultured on plastic dishes. Corneal fibroblasts cultured in a collagen matrix showed much less incorporation
of [3H]thymidine than did corneal fibroblasts cultured on plastic, and this incorporation decreased with increasing concentration
of collagen. Our present results suggest that the morphologic and biochemical characteristics of corneal fibroblasts cultured
in collagen gel are different from those cultured on plastic.
This research was supported in part by grants from the Ministry of Education, Science and Culture of Japan, by a grant from
Osaka Eye Bank, Osaka, Japan, and by an intramural research fund of Kinki University.
Part of this research was presented at the annual meeting of the Japanese Ophthalmological Society (May 1985) at Kyoto, Japan,
and at the annual meeting of the Association for Research in Vision and Ophthalmology (May 1987) at Sarasota, FL. 相似文献
11.
Alexander J. Nedopil Lydia G. Mandrussow Heike E. Daldrup-Link 《Journal of visualized experiments : JoVE》2010,(38)
The field of tissue engineering integrates the principles of engineering, cell biology and medicine towards the regeneration of specific cells and functional tissue. Matrix associated stem cell implants (MASI) aim to regenerate cartilage defects due to arthritic or traumatic joint injuries. Adult mesenchymal stem cells (MSCs) have the ability to differentiate into cells of the chondrogenic lineage and have shown promising results for cell-based articular cartilage repair technologies. Autologous MSCs can be isolated from a variety of tissues, can be expanded in cell cultures without losing their differentiation potential, and have demonstrated chondrogenic differentiation in vitro and in vivo1, 2.In order to provide local retention and viability of transplanted MSCs in cartilage defects, a scaffold is needed, which also supports subsequent differentiation and proliferation. The architecture of the scaffold guides tissue formation and permits the extracellular matrix, produced by the stem cells, to expand. Previous investigations have shown that a 2% agarose scaffold may support the development of stable hyaline cartilage and does not induce immune responses3.Long term retention of transplanted stem cells in MASI is critical for cartilage regeneration. Labeling of MSCs with iron oxide nanoparticles allows for long-term in vivo tracking with non-invasive MR imaging techniques4.This presentation will demonstrate techniques for labeling MSCs with iron oxide nanoparticles, the generation of cell-agarose constructs and implantation of these constructs into cartilage defects. The labeled constructs can be tracked non-invasively with MR-Imaging.Open in a separate windowClick here to view.(27M, flv) 相似文献
12.
Douglas R. Miller George Lust 《Biochimica et Biophysica Acta (BBA)/General Subjects》1979,583(2):218-231
Collagen metabolism was studied in degenerative articular cartilage of dogs with spontaneous, early onset osteoarthritis. A fraction of collagen which represented about 1.5.% of the total was extracted from cartilage samples with dilute phosphate buffer (pH 7.4) containing 0.2% sodium dodecyl sulfate. Agarose gel filtration in the presence of sodium dodecul sulfate revealed that extracts of degenerative cartilage had about 24% procollagen whereas extracts of normal samples had only 3%. The isolated procollagen fraction was rechromatographed on agarose columns in the presence of mercaptoethanol. This resulted in the identification of a collagen species which migrated between marker β and α collagen chains. The molecular weight of this collagen was estimated to be 150000. Based on incorporation of [14C]proline, its ratio of hydroxy[14C]proline to total 14C was 0.32. Procollagen was not found after limited pepsin digestion (pH 3,4°C, 16 h) of degenerative cartilage samples.Since the total collagen content (μg hydroxyproline/mg cartilage), hydroxy[14C]proline/mg cartilage, specific radioactivity of hydroxy[14C]proline (cpm/μg), in the whole cartilage, and the specific radioactivity of hydroxyproline in the extractable collagen fraction were similar for normal and degenerative cartilage we propose that procollagen accumulated in the degenerative cartilage due to a partial defect in conversion of procollagen to collagen. 相似文献
13.
Yusuke Ogata Yo Mabuchi Mayu Yoshida Eriko Grace Suto Nobuharu Suzuki Takeshi Muneta Ichiro Sekiya Chihiro Akazawa 《PloS one》2015,10(6)
Mesenchymal stem cells (MSCs) have the ability to differentiate into a variety of lineages and to renew themselves without malignant changes, and thus hold potential for many clinical applications. However, it has not been well characterized how different the properties of MSCs are depending on the tissue source in which they resided. We previously reported a novel technique for the prospective MSC isolation from bone marrow, and revealed that a combination of cell surface markers (LNGFR and THY-1) allows the isolation of highly enriched MSC populations. In this study, we isolated LNGFR+ THY-1 + MSCs from synovium using flow cytometry. The results show that the synovium tissue contained a significantly larger percentage of LNGFR + THY-1 + MSCs. We examined the colony formation and differentiation abilities of bone marrow-derived MSCs (BM-MSCs) and synovium-derived MSCs (SYN-MSCs) isolated from the same patients. Both types of MSCs exhibited a marked propensity to differentiate into specific lineages. BM-MSCs were preferentially differentiated into bone, while in the SYN-MSC culture, enhanced adipogenic and chondrogenic differentiation was observed. These data suggest that the tissue from which MSCs are isolated should be tailored according to their intended clinical therapeutic application. 相似文献
14.
Chondrogenesis enhanced by overexpression of sox9 gene in mouse bone marrow-derived mesenchymal stem cells 总被引:25,自引:0,他引:25
Tsuchiya H Kitoh H Sugiura F Ishiguro N 《Biochemical and biophysical research communications》2003,301(2):338-343
We investigated chondrogenesis of cell-mediated sox9 gene therapy as a new treatment regimen for cartilage regeneration. pIRES2-EGFP vector containing a full-length mouse sox9 cDNA was transfected into bone marrow-derived mesenchymal stem cells (MSCs) by lipofection and chondrogenic differentiation of these cells was evaluated. In vitro high density micromass culture of these sox9 transfected MSCs demonstrated that a matrix-rich micromass aggregate with EGFP expressing MSCs was positively stained by Alcian blue and type II collagen. Next, sox9 transfected MSCs were loaded into the diffusion chamber and transplanted into athymic mice to analyze in vivo chondrogenesis. A massive tissue formation in about 2mm diameter was visible in the chamber after 4 weeks transplantation. Histological examinations demonstrated that both Alcian blue and type II collagen were positively stained in the extracellular matrix of the mass while type X collagen was not stained. These results indicated that cell-mediated sox9 gene therapy could be a novel strategy for hyaline cartilage damage. 相似文献
15.
Nancy Burton-Wurster Christine S. Hui-Chou Helen A. Greisen George Lust 《Biochimica et Biophysica Acta (BBA)/General Subjects》1982,718(1):74-84
Collagen metabolism in the focal degenerated cartilage from immature dogs with degenerative joint disease was compared with that in the adjacent ‘normal’ cartilage of the same joint surface. The deposition of collagen into the cartilage in vitro as measured by accumulation of hydroxyl [14C]proline was decreased in the early and in advanced stages of cartilage degeneration. The deposition of collagen into cartilage in vivo as measured by the accumulation of hydroxy[3H]proline (intravenously injected [3H]proline) also was reduced in the degenerated cartilages of a dog with degenerative joint disease. Gel electrophoretic analysis revealed that degenerated cartilage contained less α1 collagen chains, but increased amounts of larger proteins. Degenerated cartilage contained more water, increased amounts of unidentified, non-collagenous protien. increased collagenolytic enzyme activity and fewer chondrocytes. Decreased deposition of collagen would result in collagen depletion in the foci of degenerated cartilage in joints of dogs with degenerative joint disease. 相似文献
16.
17.
Molecular and cellular characterization during chondrogenic differentiation of adipose tissue-derived stromal cells in vitro and cartilage formation in vivo 总被引:13,自引:0,他引:13
Lin Y Luo E Chen X Liu L Qiao J Yan Z Li Z Tang W Zheng X Tian W 《Journal of cellular and molecular medicine》2005,9(4):929-939
Human adipose tissue is a viable source of mesenchymal stem cells (MSCs) with wide differentiation potential for musculoskeletal tissue engineering research. The stem cell population, termed processed lipoaspirate (PLA) cells, can be isolated from human lipoaspirates and expanded in vitro easily. This study was to determine molecular and cellular characterization of PLA cells during chondrogenic differentiation in vitro and cartilage formation in vivo . When cultured in vitro with chondrogenic medium as monolayers in high density, they could be induced toward the chondrogenic lineages. To determine their ability of cartilage formation in vivo , the induced cells in alginate gel were implanted in nude mice subcutaneously for up to 20 weeks. Histological and immunohistochemical analysis of the induced cells and retrieved specimens from nude mice at various intervals showed obviously cartilaginous phenotype with positive staining of specific extracellular matrix (ECM). Correlatively, results of RT-PCR and Western Blot confirmed the expression of characteristic molecules during chondrogenic differentiation namely collagen type II, SOX9, cartilage oligomeric protein (COMP) and the cartilage-specific proteoglycan aggrecan. Meanwhile, there was low level synthesis of collagen type X and decreasing production of collagen type I during induction in vitro and formation of cartilaginous tissue in vivo . These cells induced to form engineered cartilage can maintain the stable phenotype and indicate no sign of hypertrophy in 20 weeks in vivo , however, when they cultured as monolayers, they showed prehypertrophic alteration in late stage about 10 weeks after induction. Therefore, it is suggested that human adipose tissue may represent a novel plentiful source of multipotential stem cells capable of undergoing chondrogenesis and forming engineered cartilage. 相似文献
18.
David E. Woolley John M. Evanson 《Biochimica et Biophysica Acta (BBA)/General Subjects》1977,497(1):144-150
No significant inhibition of purified rheumatoid synovial collagenase was found when this enzyme was assayed in the presence of porcine or human cartilage proteoglycans. Reaction mixtures containing up to twice the amount of proteoglycan compared to that of collagen, w/w,, had little effect on collagen degradation as judged by the reconstituted [14C] collagen fibril assay and polyacrylamide gel electrophoresis. Proteoglycans were not degraded by the synovial collagenase preparation. Although the human collagenases derived from rheumatoid synovium, gastric mucosa, skin and granulocytes showed some reduction in activity when exposed to aggregated proteoglycans at high concentrations, disaggregated proteoglycans had no inhibitory effect. It is concluded that cartilage proteoglycans do not directly inhibit human collagenases in vitro, but in vivo they may provide some physical barriers which might limit the accessibility of the enzyme to its collagen substrate. 相似文献
19.
Previous studies have reported that type V collagen is an anti-adhesive substrate for cultured cells in that the cells detach from culture dishes coated with type V collagen molecules or polypeptides derived from them. We have noticed that human fetal lung fibroblasts (TIG-1) initially show no reduction in adherence to and spreading on a dish coated with reconstituted type V collagen fibrils but eventually detach from the dish and form cell clumps. To determine the way in which reconstituted type V collagen fibrils are involved in cell clump formation, we have followed the fate of the fluorescence of type V collagen fibrils pre-labeled with fluorescein isothiocyanate. Essentially, all the fluorescence disappeared from the dish surface as the cells detached and was condensed in the cell clumps. The cells that were recovered from clumps and dissociated into separate cells by trypsin treatment proliferated normally after they were seeded on a bare culture dish. This result and those from gel electrophoresis, fluorescence microscopy, and a cell proliferation assay indicate that the cell detachment from the dish is not caused by cell necrosis or apoptosis but by cellular motility together with the unique features of type V collagen fibrils. Not only the adherence of type V collagen fibrils to TIG-1 cells is much stronger than that to the culture dish, but the fibrils are retained on the cellular surface. The strong adherence of type V collagen fibrils to cells plays a role in cementing TIG-1 cells together.The present study was supported in part by Grant-in-Aid for Developmental Scientific Research (07558249), by The Japan Society for the Promotion of Science, Research for the Future Program (JSPS-RFTF96I00201), by the Program for Promotion of Fundamental Studies in Health Science of the Organization for Pharmaceutical Safety and Research (OPSR), by Grant-in-Aid for the Creation of Innovations through Business-Academic-Public Sector Cooperation to T.H., and by Grant-in-Aid for Scientific Research (B) to Y.I. 相似文献
20.
Giuseppe A. Ferraro Francesco De Francesco Gianfranco Nicoletti Francesca Paino Vincenzo Desiderio Virginia Tirino Francesco D'Andrea 《Journal of cellular biochemistry》2013,114(5):1039-1049
Stem cell based therapies for the repair and regeneration of various tissues are of great interest for a high number of diseases. Adult stem cells, instead, are more available, abundant and harvested with minimally invasive procedures. In particular, mesenchymal stem cells (MSCs) are multi‐potent progenitors, able to differentiate into bone, cartilage, and adipose tissues. Human adult adipose tissue seems to be the most abundant source of MSCs and, due to its easy accessibility; it is able to give a considerable amount of stem cells. In this study, we selected MSCs co‐expressing CD34 and CD90 from adipose tissue. This stem cell population displayed higher proliferative capacity than CD34?CD90? cells and was able to differentiate in vitro into adipocytes (PPARγ+ and adiponectin+) and endothelial cells (CD31+VEGF+Flk1+). In addition, in methylcellulose without VEGF, it formed a vascular network. The aim of this study was to investigate differentiation potential of human adipose CD34+/CD90+ stem cells loaded onto commercial collagen sponges already used in clinical practice (Gingistat) both in vitro and in vivo. The results of this study clearly demonstrate that human adult adipose and loose connective tissues can be obtained in vivo, highlighting that CD34+/CD90 ASCs are extremely useful for regenerative medicine. J. Cell. Biochem. 114: 1039–1049, 2013. © 2012 Wiley Periodicals, Inc. 相似文献