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野生大豆和烟草根尖细胞分化过程中的DNA动态和细胞图像分析 总被引:1,自引:0,他引:1
分别截取野生大豆根尖分生区、伸长区和成熟区细胞,用Hoechst33258对DNA进行专一染邑,通过计算机数字图象处理系统,采用灰度测量法原位测定根尖不同区的DNA含量。同理取烟草根尖不同区细胞,用率尔根反应对DNA进行专一染色,并由与扫描显微光度计相联的数字图象处理系统原位测定备区域中细胞的DNA含量。发现从分生区到成熟区,细胞核DNA含量呈递增趋势。分化的细胞中含量异常增高而不分裂,说明DNA的动态变化与发育和分化存在着某种联系。细胞图象分析的结果表明三个区的细胞面积、最大径、最小径及形态因子也沿分生区到成熟区呈递增趋势。 相似文献
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LHRH刺激雄性大鼠垂体LH分泌高峰时细胞形态变化的图象分析 总被引:3,自引:1,他引:2
应用ABC亲和组织化学顺序、MIS-1计划机图象处理系统测定外源性LHRH刺激雄性大鼠LH分泌高峰前后LH细胞面积、细胞内液泡面积和细胞形状等形态参数的变化。结果表明:雄性大鼠LH基础分泌时,LH细胞处于贮存状态。 相似文献
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时延细胞神经网络的全局稳定性分析 总被引:9,自引:1,他引:8
本文利用 Lyapunov泛函方法和一些分析技巧得到了一类时延细胞神经网络DCNN全局渐进稳定性的若干新的判据;这些判据可用于设计出全局稳定的各种动态网络,且在信号处理,特别是动态图象处理中具有重要的意义. 相似文献
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本文报道了一种新型的低温生物学实验系统——红细胞低温显微动态图象处理系统,该系统将先进的计算机控温、图象处理技术和低温显微镜及摄录象机有机地联成一体。将此技术应用于红细胞的低温冰冻保存实验,具有能实时记录血细胞在冰冻过程中的实验参数(如时间、温度、降温速率等)与细胞图象,能对其图象处理及面积、体积测量,也能使细胞呈现伪彩色及测定红细胞冰冻损伤程度。该系统是一新的综合性的低温生物学研究仪器,对研究器官保存与移植具有明显意义。 相似文献
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鼻咽细胞的双光子显微图像中含有着丰富信息,借助计算机和图像处理算法可进行分析处理。图象分割是双光子显微图象处理中的一项重要技术,至今为止尚未形成一个最佳通用方法,也没有定义出双光子显微图象分割的统一标准。本文首先采用噪声干扰法进行去噪,采用低帽的变换等的数学形态学来增强鼻咽癌细胞图像,使细胞更加容易分辨,接着对几种经典边缘检测算法进行讨论比较,紧接着根据鼻咽双光子显微图像的实际特征,采取腐蚀算法求出鼻咽癌细胞边缘。然后进行区域生长定位细胞,并采用一些改进的判别分析算法和区域面积算法对鼻咽癌细胞进行阈值分割,获得较好结果。 相似文献
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为了合理评价化合物的促神经再生活性.用(MTT)、分化计数、图象处理等方法,借助FK506、GPI1046阳性化合物,建立了一个基于PC12细胞存活和分化的化合物筛选系统.结果表明,无论在细胞存活实验还是在分化实验中,FK506、GPI1046都可以明显增强神经生长因子(NGF)的效应,即促神经再生的作用.也就是说,这一系统将有助于从组合化学方法合成的化合物文库中,筛选出具有促神经再生活性的化合物. 相似文献
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High Throughput Screening of Gene Expression Signatures 总被引:1,自引:0,他引:1
This paper focuses on microarray image analysis and discusses a completely automated approach to image processing, which eliminates human intervention. A system for automated image processing is described, which is capable of processing image files in a batch-mode thus allowing high-throughput of microarray image analysis. Grid-placement and spot finding are achieved without operator's help. The software eliminates noise signals from the data analysis process and minimizes operator's involvement in the procedure. 相似文献
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应用扫描电镜和图像分析方法对宽带噪声损伤后鸡基底乳头病变以及听觉毛细胞再生反应进行定量研究。观察了存活0、2、7、15和30天时平均毛细胞表面积,平均毛细胞密度,毛细胞/支持细胞面积比及基底乳头损伤范围等参数。结果显示,基底乳头病变范围为近端10%至中段60%。早期反应包括支持细胞扩张和毛细胞静纤毛变性及皮板表面积缩小。损伤后2天可见新生毛细胞,形态与胚胎期幼稚毛细胞相似。7~15天毛细胞数目与表面积逐渐增加,基底乳头mosaic构型基本恢复。静纤毛排列与极性至30天时尚未完全恢复。损伤区仍有散在的残存毛细胞及分化早期的幼稚毛细胞。上述结果提示,再生毛细胞的前体存在于受损区局部并与支持细胞的活动有关。前体细胞的增殖至再生毛细胞出现的时间约为48h,但亦存在一定程度的非同步现象,损伤后7~15天为细胞修复的主要时期。 相似文献
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An image acquisition and processing system has been developed for quantitative microscopy of absorption or fluorescence in stained cells. Three different light transducers are used in the system to exploit the best characteristics of these sensors for different biological measurements. A digital scanner, in the form of a linear array charge-coupled device (CCD), acquires data with high spatial and photometric resolution. A color (RGB) camera is employed when spectral information is required for the segmentation of cellular subcomponents. An image-intensified charged-injection device (CID) camera provides for very low light intensity measurements, primarily for fluorescence-labeled cells. Properties of these transducers, such as contrast transfer function, linearity, and photo-response nonuniformity, have been measured. Two dedicated image processing units were incorporated into the system. The front-end processor, based on a digital signal processor, provides functions such as object detection, raw image calibration, compression, artifact removal, and filtering. The second image processor is associated with the frame memory and includes a histogram processor, a dedicated arithmetic logic unit for image processing functions, and a graphics module for one-bit overlay functions. An interactive program was developed to acquire cell images and to experiment with a range of segmentation algorithms, feature extractions, and other image processing functions. The results of any image operation are displayed on the video monitor. Once a desired processing sequence is determined, the sequence may be stored to become part of a command library and can be executed thereafter as a single instruction. 相似文献
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Background
Automated image analysis on virtual slides is evolving rapidly and will play an important role in the future of digital pathology. Due to the image size, the computational cost of processing whole slide images (WSIs) in full resolution is immense. Moreover, image analysis requires well focused images in high magnification.Methods
We present a system that merges virtual microscopy techniques, open source image analysis software, and distributed parallel processing. We have integrated the parallel processing framework JPPF, so batch processing can be performed distributed and in parallel. All resulting meta data and image data are collected and merged. As an example the system is applied to the specific task of image sharpness assessment. ImageJ is an open source image editing and processing framework developed at the NIH having a large user community that contributes image processing algorithms wrapped as plug-ins in a wide field of life science applications. We developed an ImageJ plug-in that supports both basic interactive virtual microscope and batch processing functionality. For the application of sharpness inspection we employ an approach with non-overlapping tiles. Compute nodes retrieve image tiles of moderate size from the streaming server and compute the focus measure. Each tile is divided into small sub images to calculate an edge based sharpness criterion which is used for classification. The results are aggregated in a sharpness map.Results
Based on the system we calculate a sharpness measure and classify virtual slides into one of the following categories - excellent, okay, review and defective. Generating a scaled sharpness map enables the user to evaluate sharpness of WSIs and shows overall quality at a glance thus reducing tedious assessment work.Conclusions
Using sharpness assessment as an example, the introduced system can be used to process, analyze and parallelize analysis of whole slide images based on open source software.16.
Morphometric requirements for image analysis and the IBAS interactive automatic image analysis system 总被引:1,自引:0,他引:1
H Schwarz 《Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology》1986,8(3):267-270
The morphometric demands made on an image analysis system are discussed, as is the IBAS interactive automatic system, which meets those requirements. The modular design of the IBAS image analysis system, with its tailor-made processors for image processing, system control and pattern recognition, gives speed and flexibility. The IMAGE image analysis language guarantees user friendliness, and, last but not least, the enormous amount of software offers accurate, reproducible measurements and dedicated evaluation programs. 相似文献
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《Journal of Asia》2014,17(4):685-694
This paper presents a new system, based on digital image processing, to quantify whiteflies on soybean leaves. This approach allows counting to be fully automated, considerably speeding up the process in comparison with the manual approach. The proposed algorithm is capable of detecting and quantifying not only adult whiteflies, but also specimens in the nymph stage. A complete performance evaluation is presented, with emphasis on the conditions and situations for which the algorithm succeeds, and also on the circumstances that need further work. Although this proposal was entirely developed using soybean leaves, it can be easily extended to other kinds of crops with little or no changes in the algorithm. The system employs only widely used image processing operations, so it can be easily implemented in any image processing software package. 相似文献
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Image analysis as a tool for quantitative phycology: a computational approach to cyanobacterial taxa identification 总被引:1,自引:0,他引:1
In the following work we discuss the application of image processing and pattern recognition to the field of quantitative
phycology. We overview the area of image processing and review previously published literature pertaining to the image analysis
of phycological images and, in particular, cyanobacterial image processing. We then discuss the main operations used to process
images and quantify data contained within them. To demonstrate the utility of image processing to cyanobacteria classification,
we present details of an image analysis system for automatically detecting and classifying several cyanobacterial taxa of
Lake Biwa, Japan. Specifically, we initially target the genus Microcystis for detection and classification from among several species of Anabaena. We subsequently extend the system to classify a total of six cyanobacteria species. High-resolution microscope images containing
a mix of the above species and other nontargeted objects are analyzed, and any detected objects are removed from the image
for further analysis. Following image enhancement, we measure object properties and compare them to a previously compiled
database of species characteristics. Classification of an object as belonging to a particular class membership (e.g., “Microcystis,”“A. smithii,”“Other,” etc.) is performed using parametric statistical methods. Leave-one-out classification results suggest a system error rate
of approximately 3%.
Received: September 6, 1999 / Accepted: February 6, 2000 相似文献
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Many image analysis systems are available for processing the images produced by laser scanning of DNA microarrays. The image processing system takes pixel-level intensity data and converts it to a set of gene-level expression or copy number summaries that will be used in further analyses. Image analysis systems currently in use differ with regard to the specific algorithms they implement, ease of use, and cost. Thus, it would be desirable to have an objective means of comparing systems. Here we describe a systematic method of comparing image processing results produced by different image analysis systems using a series of replicate microarray experiments. We demonstrate the method with a comparison of cDNA microarray data generated by the UCSF Spot and the GenePix image processing systems. 相似文献
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Electron crystallography of membrane proteins determines the structure of membrane-reconstituted and two-dimensionally (2D) crystallized membrane proteins by low-dose imaging with the transmission electron microscope, and computer image processing. We have previously presented the software system 2dx, for user-friendly image processing of 2D crystal images. Its central component 2dx_image is based on the MRC program suite, and allows the optionally fully automatic processing of one 2D crystal image. We present here the program 2dx_merge, which assists the user in the management of a 2D crystal image processing project, and facilitates the merging of the data from multiple images. The merged dataset can be used as a reference to re-process all images, which usually improves the resolution of the final reconstruction. Image processing and merging can be applied iteratively, until convergence is reached. 2dx is available under the GNU General Public License at http://2dx.org. 相似文献