The effects of intraperitoneally implanted dummy acoustic transmitters on the behaviour and physiology of juvenile Atlantic salmon, Salmo salar L

The behavioural and physiological effects of surgical implantation of dummy miniature acoustic transmitters into the peritonealcavities ofjuvenile Atlantic salmon, Salmo salar L., were assessed. lntraperitoneal implantations had no significant effect on growth, feeding or swimming behaviour in either parr or smolts. Recovery from the surgical implantation was both rapid and total; infection was absent; and physiological processes such as smoltification and maturation of testes in precocious parr were unaffected. Expulsion of the transmitter through the body wall, not through the implantation wound, occurred in a number of fish but without adversely affecting the animals. The intraperitoneal implantation technique is discussed in relation to its use during biotelemetry studies.     

Body and scale growth of wild Atlantic salmon smolts during seaward emigration

The relationship between scale and body growth for emigrating Atlantic salmon, Salmo salar, smolts was previously not understood and therefore was examined in this study using mark-recapture techniques. The size of smolts at time of recapture was significantly greater than when marked (P = 0.0002). The growth in length of smolts emigrating 5 km over an average of 20 days was 7.7 ± 6.1 mm per day. Instantaneous somatic growth (G _body) ranged from 7.0 × 10⁻⁴ to 5.1 × 10⁻³ (mean = 2.7 × 10⁻³ ± 1.3 × 10⁻³). The mean number of plus growth circuli present per scale was significantly greater for smolts when recaptured compared to when marked (P = 0.0014). The instantaneous growth rate of scales (G _scale) ranged from 1.4 × 10⁻³ to 11.5 × 10⁻³ (mean = 6.6 × 10⁻³ ± 4.3 × 10⁻³). The relationship between body size and scale radius showed positive allometry rather than isometry. The relationship of G _scale with G _body showed positive allometry indicating that scales grew at a slightly faster rate than the body during the emigratory period.     

The effect of intragastric transmitters on feeding behaviour of Atlantic salmon, Salmo salar; parr during autumn

The proportion of Atlantic salmon parr (146 265 mm total length) that fed in laboratory tanks during autumn was 0% in fish filled with intragastric transmitters compared with 78 100% in controls.     

Use of radio telemetry and electrofishing to assess spawning by transplanted Atlantic salmon

The river Ingdalselva, which drains to the Trondheims fjord, has no local salmon population due to an impassable waterfall 500 m upstream from the outlet. In the period 1994–97, a total of 31 mature Atlantic salmon (19 females and 12 males) from the rivers Orkla (1994–96) and Vigda (1997) were radio-tagged and released in the river Ingdalselva before spawning. The main goal of the project was to find out whether the fish would stay and spawn in the river, and if the observations during the spawning period could be used to indicate where spawning had taken place. Some fish left the river shortly after release, but 77% of the fish stayed in the river during the spawning period in October. Most of the females (74%) spawned in the river, including multi-sea winter salmon of approximately 10 kg. Some fish remained at the site of the release, while others migrated downstream to hiding places where they stayed until spawning. Long distance upstream migrations were not observed. The observations of the fish during the spawning period were used to identify spawning areas. Electrofishing for salmon fry in the spawning areas the following year in all cases produced fry, and in two of the years salmon fry were also found in areas where no spawning activity had been registered. Radio-tracking was an efficient method for determining whether transplanted salmon would remain in a `new'n river during the spawning season and for locating spawning areas, particularly when the fish were tracked daily.     

Spawning behaviour of a farmed escaped female Atlantic salmon (Salmo salar)

Excavation of stranded redds revealed differences in spawning behaviour between farmed and wild Atlantic salmon. The redd of a farmed fish contained more egg pockets (nine v . average of two) and fewer eggs per pocket (averages: 459 v . 707). No other pocket measures differed.     

The feeding behaviour of juvenile Atlantic salmon in relation to turbulent flow

The feeding behaviour of juvenile Atlantic salmon Salmo salar in the Sainte‐Marguerite River, Quebec, Canada, varied with the characteristics of turbulent flow. Simulations indicated that juveniles would decrease their swimming costs during attacks by 19·8% in low and by 31·1% in high turbulent conditions by initiating movements in low‐speed flow events. The real swimming costs did not differ from the swimming costs estimated for a situation where fish initiate their movements at randomly selected flow velocities. The juvenile Atlantic salmon did not seem to prefer low‐speed flow events when initiating their movements. The proportion of time used for movements by fish decreased with an increase in the mean and the s . d . of the flow velocity.     

Effects of surgically implanted transmitters on swimming performance, food consumption and growth of wild Atlantic salmon parr

Experiments were conducted on wild Atlantic salmon Salmo salar parr to determine the effect of surgically implanted dummy transmitters on swimming performance, food consumption and growth. Swimming performance of tagged fish (tag 1·7–3·7% of fish mass) was similar to that of control fish 1, 5 and 10 days after surgery. Negative effects on growth, however, were found up to day 36 of a 45 day experiment (tag 0·9–2·6% of fish mass). Consumption rates were similar between tagged and control fish and did not explain differences in growth.     

Migratory and reproductive behaviour of female adult Atlantic salmon, Salmo salar L., in a spawning stream

Migration and spawning behaviour of eight Atlantic salmon adult females were analysed by radio-tracking in relation to the degree of sexual maturity in a spawning tributary of the R. Sélune. Six of them were grilse and four of them were ripe. All the fish migrated upstream until reaching their spawning site at a distance of 4–12 km from the trap. The daily migration rate up to this site was inversely correlated with the length of the female. Spawning occurred in all fish at the same time when the water temperature increased dramatically. Spawning lasted 1–10 days according to the fish. After spawning, females quickly moved downstream only small distances and then stayed in approximately the same location until death. This study concluded that ripeness did not influence behaviour on the spawning migration and describes certain characteristics of the reproductive phase in a spawning tributary.     

Effect of increased flow on the behaviour of Atlantic salmon parr in winter

The effect of increased flow on movement and microhabitat use of Atlantic salmon Salmo salar parr in winter was investigated using radiotelemetry. To simulate hydropeaking operations, flow was increased four‐fold from 1·3 m³ s⁻¹ to 5·2 m³ s⁻¹ for 24 h periods. Flow did not affect fish habitat use or displacement and had little effect on fish activity within diel periods. During high flow periods in late winter, fish reduced night‐time activity. Stranding rates during flow reduction were also very low (only one fish).     

Assessment of a retrofitted downstream fish bypass system for wild Atlantic salmon (<Emphasis Type="Italic">Salmo salar</Emphasis>) smolts and kelts at a hydroelectric facility on the Exploits River,Newfoundland, Canada

In 2002 and 2003, the Bishops Falls hydroelectric generating facility on the Exploits River, insular Newfoundland, Canada, underwent extensive refurbishing including replacement of turbines and installation of a ‘retrofitted’ bypass and fish handling system. The effectiveness of this new bypass system has been assessed during the annual downstream run of wild Atlantic salmon smolt and kelt in 2003 and 2004. In 2003, 195 smolt were radio tagged and released between June 9 and July 2, in the forebay of the hydro plant (19 releases) and one upstream (in-river) release. Fish guidance efficiency (FGE) of the system overall was 63% (123 of 195 fish) with 36 fish passing through the turbines, and six known mortalities. In 2004, between June 9 and July 2, a total of 358 smolt and 103 kelt were released in the forebay in 45 and 13 releases (n = 8 per release), respectively. The FGE of the system for smolt was 71.7% (257 of 358 fish) and for kelt was 92.3% (95 of 103 fish). In 2004, 96 tagged smolt passed through the turbines and 43 (44.8%) were detected at a downstream station confirming they had survived turbine passage, suggesting an overall survival of smolt passage of the Bishops Falls hydro facility in the order of 85%. A total of seven kelts (6.8%) passed through the turbines and were not detected 1.5 km downstream suggesting they did not survive turbine passage. Smolt spent on average 39.8 h in the forebay before exiting in 2003 and forebay residency averaged 26 h in 2004. In both years, most smolt selected their passage route, actively or passively, within the first 10 h with secondary peaks at 25–30 h and 50–55 h, corresponding to evening passage in the second and third night, after release. Few smolt were bypassed or entrained into turbines during daylight hours. In both years turbine passed smolt spent more time in the forebay suggesting the longer fish reside in the forebay the greater the likelihood of turbine entrainment. Kelt were either bypassed or turbine entrained relatively quickly, within 2 h of release, and virtually all kelts were bypassed/turbine entrained during the hours of 18:00 and 01:00. These data on fish behaviour and residency in the forebay will assist further refinement of operations of the bypass facility to optimize survival.     

Genetic studies on serum transferrins in Atlantic salmon

Published and unpublished data on genetic variation at the transferrin locus ( TF *) in Atlantic salmon from rivers in eastern North America sampled from 1968 to 1970 were reanalysed and compared with data for samples collected in 1998 from nine of the same rivers. Genetic differentiation among rivers was highly significant as was spatial differentiation among tributary samples within the Miramichi River system, the largest rivers studied. Comparison of allele frequencies in rivers also sampled in 1998 show no overall evidence of significant genetic change after 30 years, spanning 9 generations. The results strongly support the stability of the patterns of spatial genetic differentiation and support the occurrence in Atlantic salmon of reproductive isolation among rivers and among tributaries within large river systems.     

Bimodal size distributions in wild juvenile Atlantic salmon populations and their relationship with age at smolt migration

A bimodal length distribution developed in an Atlantic salmon population planted as start-fed fry in a Norwegian stream. The earliest fish to smolt were from the upper modal group.     

Fatty acid profiles of wild brown trout and Atlantic salmon juveniles in the Nivelle basin

Samples of Atlantic salmon Salmo salar 0+ year parr and brown trout Salmo trutta 1+ year juveniles, and 2+ and 3+ year sub‐adults were obtained from nine sites of the Nivelle River, France, located at the southern edge of the species distribution area. Fatty acid composition of the different samples were analysed. Within the same river basin, the fatty acid profiles appeared to vary among samples from different locations. Based on cluster analysis, fatty acid signatures of both species appeared to discriminate samples from different locations.     

The use of genetic marking to assess the reproductive success of mature male Atlantic salmon parr (Salmo salar, L.) under natural spawning conditions

The reproductive success of mature male Atlantic salmon parr ( Salmo salar ) under natural spawning conditions was estimated using the polymorphism at the MEP - 2 * locus as a genetic marker. The percentage of eggs per redd fertilized by parr varied considerably over the five redds examined (0·9–27.7%, mean 10·8%), but a gametic contribution from mature parr was detected in each case. Parr reproductive success has important implications for the population structure and evolution of the Atlantic salmon through its effect on gene flow.     

Endurance swimming of diploid and triploid Atlantic salmon

When groups of diploid (mean ±  s . e . fork length, L _F) 33·0 ± 1·4 cm and triploid (35·3 ± 0·5 cm) Atlantic salmon Salmo salar were forced to swim at controlled speeds in a carefully monitored 10 m diameter 'annular' tank no significant difference was found between the maximum sustained swimming speeds ( U _ms, maintainable for 200 min) where the fish swam at the limit of their aerobic capability. Diploids achieved 2·99 body lengths per second (bl s⁻¹)(0·96 m s⁻¹) and triploids sustained 2·91 bl s⁻¹(1·02 m s⁻¹). The selection of fish for the trials was based on their ability to swim with a moving pattern projected from a gantry rotating at the radius of the tank and the selection procedure did not prove to be significant by ploidy. A significant difference was found between the anaerobic capabilities of the fish measured as endurance times at their prolonged swimming speeds. During the course of the experimentation the voluntary swimming speed selected by the fish increased and the schooling behaviour improved. The effect of the curvature of the tank on the fish speeds was calculated (removing the curved effect of the tank increased the speed in either ploidy by 5·5%). Implications of the endurance times and speeds are discussed with reference to the aquaculture of triploid Atlantic salmon.     

Expression of olfactory receptors in different life stages and life histories of wild Atlantic salmon (Salmo salar)

It has been hypothesized that salmonids use olfactory cues to return to their natal rivers and streams. However, the key components of the molecular pathway involved in imprinting and homing are still unknown. If odorants are involved in salmon homing migration, then olfactory receptors should play a critical role in the dissipation of information from the environment to the fish. Therefore, we examined the expression profiles of a suite of genes encoding olfactory receptors and other olfactory-related genes in the olfactory rosettes of different life stages in two anadromous and one non-anadromous wild Atlantic salmon populations from Newfoundland, Canada. We identified seven differentially expressed OlfC genes in juvenile anadromous salmon compared to returning adults in both populations of anadromous Atlantic salmon. The salmon from the Campbellton River had an additional 10 genes that were differentially expressed in juveniles compared to returning adults. There was no statistically significant difference in gene expression of any of the genes in the non-anadromous population (P < 0.01). The function of the OlfC gene products is not clear, but they are predicted to be amino acid receptors. Other studies have suggested that salmon use amino acids for imprinting and homing. This study, the first to examine the expression of olfactory-related genes in wild North American Atlantic salmon, has identified seven OlfC genes that may be involved in the imprinting and homeward migration of anadromous Atlantic salmon.     

The impact of a brominated flame retardant on smoltification and olfactory function in Atlantic salmon (Salmo salar L.) smolts

Despite a reduction in exploitation of salmon stocks throughout the NE Atlantic, there continues to be a decline in many populations. The factors regulating these populations remain poorly understood, although there is evidence that environmental conditions experienced in freshwater can effect survival in the marine environment. The aim of this study was to investigate the impact of a brominated flame retardant (hexabromocyclododecane, HBCD) on the parr-smolt transformation in juvenile salmon. It is during this pre-adaptive period to marine life that olfactory imprinting to the natal river is considered to occur. Fish were exposed to low levels of HBCD for 30 days over the peak smoltification period in freshwater, and then transferred to clean seawater for 20 days. Fish were sampled weekly to assess changes in some of the physiological parameters associated with smoltification, and olfactory response to conspecific smolt urine was measured using an electro-olfactogram (EOG). Exposure to HBCD did not affect seawater adaptability, although there was some disruption of plasma thyroid hormone levels, as well as a reduction in olfactory function to conspecific smolt urine. The results are discussed in relation to the marine survival and successful homing of adult salmon.     

Effects of courtship and relative mate size on sexual motivation in Atlantic salmon

Both sexes of Atlantic salmon Salmo salar were very sensitive to the absence of their mate on the spawning ground, particularly females during the last hour before oviposition. The improvement of behavioural chaining just before viposition might elicit the accurate timing of synchronized gamete release for successful fertilization. While the reproductive behaviour of the opposite sex could largely affect breeding activity, relative mate size appeared to be the prevailing sexual motivation factor in this species. Even in the absence of courtship, large mate size may constitute a supra-stimulus inducing an increase in spawning behaviour of the other sex. Females with smaller males delayed their first spawning activity, took longer to spawn and made more nests than those with large males. However, female egg retention was not influenced by relative male size.