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1.
Abstract We report the identification of an open reading frame in a serogroup B isolate of Neisseria meningitidis that exhibits high nucleotide and predicted amino acid identity with the fpg gene of Escherichia coli , and its product, formamidopyrimidine-DNA glycosylase (Fapy-DNA glycosylase), a DNA repair enzyme. We further show that the meningococcal fpg is co-transcribed with nlaA , encoding a lysophosphatidic acid acyltransferase, and suggest that the DNA repair enzyme may be involved in the regulation of nlaA or its gene product. 相似文献
2.
In vitro treatment with concentrated growth factors (CGF) and sodium orthosilicate positively affects cell renewal in three different human cell lines 下载免费PDF全文
Veronica Bonazza Elisa Borsani Barbara Buffoli Silvia Parolini Francesco Inchingolo Rita Rezzani Luigi Fabrizio Rodella 《Cell biology international》2018,42(3):353-364
3.
Benjamin Vigl 《Experimental cell research》2009,315(2):336-1187
Lymphatic vessels play an important role in tissue fluid homeostasis, intestinal fat absorption and immunosurveillance. Furthermore, they are involved in pathologic conditions, such as tumor cell metastasis and chronic inflammation. In comparison to blood vessels, the molecular phenotype of lymphatic vessels is less well characterized. Performing comparative gene expression analysis we have recently found that coxsackie- and adenovirus receptor (CAR) is significantly more highly expressed in cultured human, skin-derived lymphatic endothelial cells (LECs), as compared to blood vascular endothelial cells. Here, we have confirmed these results at the protein level, using Western blot and FACS analysis. Immunofluorescence performed on human skin confirmed that CAR is expressed at detectable levels in lymphatic vessels, but not in blood vessels. To address the functional significance of CAR expression, we modulated CAR expression levels in cultured LECs in vitro by siRNA- and vector-based transfection approaches. Functional assays performed with the transfected cells revealed that CAR is involved in distinct cellular processes in LECs, such as cell adhesion, migration, tube formation and the control of vascular permeability. In contrast, no effect of CAR on LEC proliferation was observed. Overall, our data suggest that CAR stabilizes LEC-LEC interactions in the skin and may contribute to lymphatic vessel integrity. 相似文献
4.
Effects of a 50 Hz sinusoidal magnetic field on cell adhesion molecule expression in two human osteosarcoma cell lines (MG-63 and Saos-2) 总被引:1,自引:0,他引:1
Santini MT Rainaldi G Ferrante A Indovina PL Vecchia P Donelli G 《Bioelectromagnetics》2003,24(5):327-338
The possibility that a sinusoidal 50 Hz magnetic field with a magnetic flux density of 0.5 mT can induce variations in the expression of cell adhesion molecules (CAMs) in two human osteosarcoma cell lines (MG-63 and Saos-2) was investigated. In particular, the expression of two important integrins, VLA-2, the receptor for collagen, and VLA-5, the receptor for fibronectin, as well as CD44, were examined in both cell lines after these had been exposed for 7 and 14 days to a 50 Hz, 0.5 mT field. Cell surface morphology (scanning electron microscopy), cell growth characteristics (growth curves and cell cycle phase distribution), and cell death (necrosis and apoptosis) were also examined. The results demonstrate that no variations in surface morphology and cell death occurred between control and exposed cells in both MG-63 and Saos-2 cells, while significant changes were noted in cell growth and fibronectin and CD44 expression in MG-63 cells. The results are discussed in view of the important role that CAMs play in controlling various cancer cell functions, particularly proliferation and metastasis. 相似文献
5.
Scism JL Laska DA Horn JW Gimple JL Pratt SE Shepard RL Dantzig AH Wrighton SA 《In vitro cellular & developmental biology. Animal》1999,35(10):580-592
Summary Cocultures of human umbilical vein endothelial cells (ECV304) and rat glioma cells (C6) from two commercial sources, American
Type Culture Collection and European Collection of Animal Cell Cultures, were evaluated as an in vitro model for the blood-brain
barrier. Monolayers of endothelial cells grown in the presence or absence of glial cells were examined for transendothelial
electrical resistance, sucrose permeability, morphology, multidrug resistance-associated protein expression, and P-glycoprotein
expression and function. Coculture of glial cells with endothelial cells increased electrical resistance and decreased sucrose
permeability across European endothelial cell monolayers, but had no effect on American endothelial cells. Coculture of European
glial cells with endothelial cells caused cell flattening and decreased cell stacking with both European and American endothelial
cells. No P-glycoprotein or multidrug resistance-associated protein was immunodetected in endothelial cells grown in glial
cell-conditioned medium. Functional P-glycoprotein was demonstrated in American endothelial cells selected in vinblastine-containing
medium over eight passages, but these cells did not form a tight endothelium. In conclusion, while European glial cells confer
blood-brain barrier-like morphology and barrier integrity to European endothelial cells in coculture, the European endothelial-glial
cell coculture model does not express P-glycoprotein, normally found at the blood-brain barrier. Further, the response of
endothelial cells to glial factors was dependent on cell source, implying heterogeneity among cell populations. On the basis
of these observations, the umbilical vein endothelial cell-glial cell coculture model does not appear to be a viable model
for predicting blood-brain barrier penetration of drug molecules. 相似文献