Serotyping of Avian Mycoplasma Species in India

Two-hundred and two strains of avian mycoplasma species belonging to 10 biotypes were typed serologically by employing disk growth inhibition (DGI) and indirect hemagglutination (IHA) tests. These could be placed into seven serotypes, namely A (80), B (50), C (3), E (34), L (13), P (4), and 1 and R (18). The figures in parentheses show the number of strains within each type. A close relationship was observed between DGI and IHA tests. The IHA test, however, was more sensitive and specific. It was also noticed that biochemically identical biotypes, namely E and G, and B and M were also found identical in serotyping, thus confirming the biochemical identity. In view of these facts, the strains of biotypes M and G were grouped under serotypes B and E, respectively. The antigenic relationships between the serotypes are also discussed.     

Adaptive evolution of water homeostasis regulation in amphibians: Vasotocin and hydrins

Volemia and osmolality homeostasis is ensured in vertebrates through neuroendocrine reflexes, involving an afferent neural branch from baro- and osmo-receptors to hypothalamus and an efferent endocrine branch from secretory neurons to target hydroosmotic cells equipped with receptors and effectors. Whereas the osmoregulatory system in the tadpole comprises three organs, namely gut, kidney and gills, as in freshwater fishes, the adult displays a quaternary strategy with gut, kidney, urinary bladder and skin. In particular, the cutaneous permeability entails a great evaporative water loss when the animal is in the open air, loss that must be compensated by water reabsorption through the nephron and the urinary bladder and mainly by water uptake through the skin. Adaptation occurred at the level of these organs by regulation of their permeability through neurohypophysial hormones. Aside from vasotocin, active on the three organs, all anuran Amphibia possess hydrin 2 (vasotocinyl-Gly), a peptide resulting from a down-regulation of provasotocin processing. Exceptionally Xenopus laevis, a permanent aquatic toad, has hydrin 1 (vasotocinyl-Gly-Lys-Arg) instead of hydrin 2. Hydrins are somewhat more active than vasotocin on water permeation of skin and bladder but are devoid of antidiuretic activity. Adaptive evolution has created, along with the vasotocin-nephron system, preserved in all terrestrial non-mammalian tetrapods, additional functions such as the hydrin-skin and hydrin-bladder rehydration mechanisms. Specific hydrin receptors might exist in the skin and the bladder, different from those of vasotocin in the kidney. It is assumed that the water channel recruitment mechanism, found for vasopressin acting on the collecting duct principal cells in mammals, is also involved when vasotocin and hydrins stimulate their hydroosmotic target cells and that hormone-regulated aquaporin 2-like proteins could be identified in the three osmoregulatory organs of amphibians.     

Evolutionary specificity of hydrins, new hydroosmotic neuropeptides: occurrence of hydrin 2 (vasotocinyl-Gly) in the toad Bufo marinus but not in the viper Vipera aspis

Hydrin 2 (vasotocinyl-Gly), a hydroosmotic peptide resulting from differential processing of provasotocin and recently identified in frog neurohypophysis, has been looked for in the pituitary gland of an exotic toad (Bufo marinus) and of a reptile (Vipera aspis). Hydrin 2 has been found in the amphibian but not in the reptile. This result confirms the evolutionary specificity of hydrin 2 that has been identified only in frogs and toads but not in birds and reptiles. Occurrence of hydrin 2 is explained by its regulatory function on the water permeability of the skin of anurans.     

Identification of thermophilic and marine bacilli from shallow thermal vents by restriction analysis of their amplified 16S rDNA

AIMS: In order to identify 73 thermophilic isolates from shallow, marine thermal vents of Eolian Islands, we compared their restriction patterns of amplified 16S rDNA with those of nine well described Bacillus species and eight Eolian Bacillus strains. METHODS AND RESULTS: This study allowed to assign 57 (78%) isolates to different Bacillus species. Nineteen field strains were recognised as representatives of four described species, namely B. thermodenitrificans, "B. caldolyticus", B. vulcani and B. stearothermophilus. The profiles of 38 isolates matched instead, those of seven Eolian strains (B. thermodenitrificans strain A2, B. licheniformis strain B3-15, and five novel species, represented by Bacillus strain 1bw, Bacillus strain 4-1, Bacillus strain 5-2, Bacillus strain 10-1, Bacillus strain 1as). Among the 16 unidentified isolates, seven restriction patterns were recognised. CONCLUSIONS: This study showed that restriction analysis of amplified 16S rDNA is useful for a rapid and reliable identification of strains belonging to described species as well as for recognition of new species. SIGNIFICANCE AND IMPACT OF THE STUDY: This work revealed a high taxonomic diversity among the thermophilic bacilli isolated from Eolian Islands and a distinct distribution of the species within the Eolian hydrothermal vent system.     

A Chromosomal Study of Eight Species in Allium Sect. Rhiziridium G. Don in China

The present paper reports the chromosome numbers and karyotypes of eight species of Sect. Rhiziridium in Allium (Liaceae). The materials were all collected from their natural populations in east Inner Mongolia, China. The karyotype analysis is made on the basis of Li et al. (1985).The results are as follows (for chromosomes parameters, voucher specimens and localities, see Table 1 and Plate 1--2 the idiograms of the eight species in Fig. 1): (1) Auium leucocephalum Turcz. The somatic chromosome number and karyotype of this species is 2n=16=12m=2sm+2st (2SAT), in Stebbinsl(1971) kayotype classification, which belongs to 2A (Plate 1: 1; Fig. 1: 1). The range of chromosome relative length varies between 8.90--15.55%. Two small satellites are attached to the short arms of the 8th pair of chromosomes. (2) A. strictum Schrader has 2n (4x) =32=16m+4sm+12st, belonging to 2B (Plate 1: 2 & Fig. 1: 2). Satellites were not observed., and the range of chromosome relative length is between 3. 67-11.00%. (3) A. ramosum L. 2n=16=14m+ 2st (2SAT), belonging to 2A (Plate 1: 3 & Fig. 1: 3), Two small satellies are attached to the short arms of the 8th pair of chromosomes. The range of chromosome relative length is between 9.17-16.39%. The chromosome number and karyotype of this species are in accordancewith those reported by Li et al. (1982) with the material from Jinshan, Beijing. (4) A. bidentatum Fisch. ex Prokh. 2n (4x) =32=24m+4sm+4T, belonging to 2B (Plate 1: 4 & Fig. 1: 4). Satellites were not observed. A small median B-chromosome was found in root-tip cells of the population growing in sandy soil, and it is the first discovery (Plate 2: 9). The species has terminal chromosomes, which are seldom seen in Sect. Rhiziridium. The range of chromosome relative length is between 3.32—9.06%. (5) A. tenuissimu L. 2n=16= 10m+4sm+2st(2SAT), belonging to 2B(Plate 1:5 & Fig. 1:5). Two large satellites are attached to the short arms of the 8th pair of chromosome. The range of chromosome relative length is between 8.27--17.56%. (6)A. anisopodium Ledeb. 2n = 16 = l2m +2sm + 2st (2SAT), belonging to 2A (Plate 2:7 & Fig. 1: 7). Two large satellites are attached to the short arms of the 8th pair of chromosomes. In somatic cells of some plants of this species, a small submedian B-chromosome was found (Plate 2: 10, 11). The range of chromosome relative length is between 8.05-17.08 %. (7) A. anisopodium Ledeb. var. zimmermannianum (Gilg) Wang et Tang 2n (4x)=32=24m+4sm+4st( 4SAT), belonging to 2A (Plate 1: 6 & Fig. 1: 6). Four large satellites are attached to the short arms of the 15 and 16th pairs of chromosomes. The range of chromosome relative length is between 4.45--8.35%. This variety is similar to A. anisopodium Ledeb. in morphological characters, and their karyotype formulas are also very similar. The present authors consider that the variety is an allotetraploid derived from A. anisopodium Ledeb. (8) A. condensatum Turcz. 2n=16=14m+2st (2SAT), belonging to 2B (Plate 2:8 & Fig. 1:8). Two. small satellites are attached to the short arms of the 6th pair of chromosomes. In a few individuals of this species median (M) B-chromosome was discovered, and the number is stable (Plate 2: 12). The range of chromosome relative length is between 7.64--17.07%. In short, the chromosome numbers of the species studied in the present work are found to be 2n=16 or 32, and the karyotypes belong to 2A or 2B, highly symmetrical. The karyotypes of Chinese materials of these species are mostly reported for the first time. Threespecies have B-chromosomes.     

Replication origin of mitochondrial DNA in insects

The precise position of the replication origin (O(R)) of mtDNA was determined for insect species belonging to four different orders (four species of Drosophila, Bombyx mori, Triborium castaneum, and Locusta migratoria, which belong to Diptera, Lepidoptera, Coleoptera, and Orthoptera, respectively). Since the free 5' ends of the DNA strands of mtDNA are interpreted as the O(R), their positions were mapped at 1-nucleotide resolution within the A + T-rich region by using the ligation-mediated PCR method. In all species examined, the free 5' ends were found within a very narrow range of several nucleotides in the A + T-rich region. For four species of Drosophila, B. mori, and T. castaneum, which belong to holometabolous insects, although the O(R)'s were located at different positions, they were located immediately downstream of a series of thymine nucleotides, the so-called T-stretch. These results strongly indicate that the T-stretch is involved in the recognition of the O(R) of mtDNA at least among holometabolous insects. For L. migratoria (hemimetabolous insect), on the other hand, none of the long stretches of T's was found in the upstream portion of the O(R), suggesting that the regulatory sequences involved in the replication initiation process have changed through insect evolution.     

Rhizobium selenireducens sp. nov.: A Selenite-Reducing α-Proteobacteria Isolated From a Bioreactor

A Gram-negative, nonpigmented bacterium designated strain B1 was isolated from a laboratory bioreactor that reduced selenate to elemental red selenium (Se(0)). 16S rRNA gene-sequence alignment identified the isolate as a Rhizobium sp. belonging to the Rhizobium clade, which includes R. daejeonense, R. giardinii, R. undicola, R. larrymoorei, R. radiobacter, R. rubi, and R. vitis. R. radiobacter and R. rubi are its closest relatives as indicated by 16S rRNA gene-sequence alignments, which differ from strain B1 by 2.6% and 2.8%, respectively. Within this group, strains that show variances > 0.8% to 2.2% have been classified as different species. The major cellular fatty acids present in the B1 strain were C16:0 (1.8%), C18:0 (3.38%), 18:0 3-OH (1.6%), 18:1 omega7c (86.8%), 19:0 cycloomega8c (1.5%), and summed features 2 (3.8%) and 3 (1.2%). The large amount of 18:1 omega7c present is constant with members of this group of bacteria, but the small amounts of 16:0, 19:0 cycloomega8c, and summed feature 3 shows variance from R. radiobacter and R. rubi. The strain's phenotypic and biochemical characteristics are consistent with its placement in this genus.     

Two new species of African Haemaphysalis ticks (Acari: Ixodidae), carnivore parasites of the H. (Rhipistoma) leachi group

Two new tick species belonging to the African Haemaphysalis (Rhipistoma) leachi subgroup, namely H. (R.) colesbergensis n. sp. and H. (R.) oliveri n. sp., are described. Haemaphysalis (R.) colesbergensis adults are easily differentiated from the other species of the H. (R.) leachi subgroup, including H. (R.) oliveri, by the spur on coxa IV, which is considerably longer than that on coxa III. The adults of the 2 new species are equal in size, but the dental formula of the hypostome of H. (R.) colesbergensis is 4/4 compared to 5/5 for H. (R.) oliveri. The dental formula of H. (R.) oliveri also distinguishes it from other ticks in the subgroup, namely H. (R.) leachi, H. (R.) elliptica, H. (R.) moreli, and H. (R.) punctaleachi (4/4 in these species), but not from H. (R.) paraleachi, which has a 5/5 dental arrangement. However, the average total length and width of H. (R.) oliveri males (2.47 x 1.20 mm) are considerably shorter and narrower than those of H. (R.) paraleachi males (3.81 x 1.79 mm). Similar differences in size apply to the females. Nymphs and larvae of H. (R.) colesbergensis and H. (R.) oliveri can be distinguished from those of other members of the H. (R.) leachi subgroup, as well as from each other, by a combination of the following characters: size and measurement ratios, length of posterodorsal and posteroventral spurs on palpal segment II, and number of denticles per file on the hypostome. Haemaphysalis (R.) colesbergensis is known only from South Africa, where it has been collected from domestic cats and dogs and medium-sized wild felids. Haemaphysalis (R.) oliveri is recorded only from Sudan, where it has been collected from small- to medium-sized wild felids and canids and an antelope. The hosts of the immature stages of H. (R.) colesbergensis are unknown, while nymphs of H. (R.) oliveri have been collected from rodents.     

Effects of H-2 complex and non-H-2 background on urethane-induced chromosomal aberrations in mice

The incidence of in vivo urethane-induced chromosomal aberrations was examined in H-2 congenic strains of mice with B10 and A backgrounds. Chromosome analysis of bone-marrow cells could divide 7 lines of A.H-2 congenic strains into 2 groups: one with a higher frequency of chromosomal aberrations such as in A/Wy (haplotype H-2a), A/J (H-2a), A.AL (H-2al) and A.TL (H-2tl), and the other consisting of A.TH (H-2t2), A.CA (H-2f), A.BY (H-2b) and A.SW (H-2s). The same tendency was also observed in the spleen cells. Among B10.H-2 congenic mice, B10.A (H-2a), B10.BR (H-2k), B10.A(3R) (H-2i3), B10.A(5R) (H-2i5) and B10.S(9R) (H-2t4) exhibited significantly higher rates of induced chromosomal aberrations than those in B10 (H-2b), B10.S (H-2s), B10.A(2R) (H-2h2), B10.A(4R) (H-2h4) and B10.S(7R) (H-2t2). To determine the effect on non-H-2 genetic backgrounds on urethane-induced chromosomal aberrations, 4 pairs of strains which have the same H-2 haplotypes, such as in B10 vs. A.BY (H-2b), B10.A vs. A/Wy (H-2a), B10.S vs. A.SW (H-2s), and B10.S(7R) vs. A.TH (H-2t2), were compared. The strains with a B10 background exhibited significantly higher frequencies of deletions and lower frequencies of exchanges than the strains with an A background. These data suggested that at least two genes are involved in the regulation of urethane-induced chromosomal aberrations in mice, one of which is mapped between the S and D regions in the H-2 complex, and another not belonging to H-2.     

Ultrastructure of Sarcocystis spp. (Protozoa: Apicomplexa) in rodents from North Sulawesi and West Java, Indonesia

Tissue cysts of the protozoan genus Sarcocystis were detected in the skeletal muscles of 16 (40%) of 40 wild rodents captured in North Sulawesi and West Java, Indonesia. Two types of cysts were found to differ in their morphological characteristics. Macroscopic and microscopic cysts bounded by thick radially-striated cyst walls were detected at both locations in a total of 13 rodents belonging to seven different species (Bunomys chrysocomus, B. fratrorum, Maxomys bartelsii, M. musschenbroekii, Paruromys dominator, Rattus xanthurus and R. exulans). The primary cyst walls contained numerous broad spatula-like protrusions and the cysts were identified as S. singaporensis Zaman and Colley, 1976. In contrast, microscopic cysts bounded by thin smooth cyst walls were detected in seven rodents belonging to three different species captured at Toraut in North Sulawesi (B. chrysocomus, B. fratrorum and P. dominator). Ultrastructural examination revealed numerous slender hair-like protrusions of their primary cyst walls. It is proposed that these cysts be named S. sulawesiensis sp. n. on the basis of their unique morphological characteristics, their intermediate host range and their limited geographic distribution. Mixed infections by both species were found in three rodent species (B. chrysocomus, B. fratrorum and P. dominator).     

西双版纳地区的陆生等足类(甲壳亚门:等足目:潮虫亚目)

本文记述西双版纳地区陆生等足类１７种,包括７新种。新种模式标本保存于中国科学院动物研究所。种类名录如下。喜阴虫科Ｐｈｉｌｏｓｃｉｄａｅ 叶肢缅甸虫Ｂｕｒｍｏｎｉｓｃｕｓ ｌｏｂａｔｕｓ Ｋｗｏｎ ＆ Ｔａｉｔｉ,１９９３,勐仑缅甸虫,新种Ｂｕｒｍｏｎｉｓｃｕｓ ｍｅｎｇｌｕｎｅｎｓｉｓ ｓｐ．ｎｏｖ．,版纳缅甸虫,新种Ｂｕｒｍｏｎｉｓｃｕｓ ｂａｎｎｉｃｕｓ ｓｐ．ｎｏｖ．,易武缅甸虫,新种Ｂｕｒ     

Immunolocalization and expression of kinin B1R and B2R receptors in human inflammatory bowel disease

Bradykinin is a mediator of inflammation, responsible for pain, vasodilation, and capillary permeability. Bradykinin receptor 1 (B(1)R) and bradykinin receptor 2 (B(2)R) are G protein-coupled receptors that mediate kinin effects. The latter is constitutive and rapidly desensitized; the former is induced by inflammatory cytokines and resistant to densensitization. The distribution of bradykinin receptors in human intestinal tissue was studied in patients with inflammatory bowel disease (IBD), namely ulcerative colitis (UC) and Crohn's disease (CD). Both B(2)R and B(1)R proteins are expressed in the epithelial cells of normal and IBD intestines. B(1)R protein is visualized in macrophages at the center of granulomas in CD. B(2)R protein is normally present in the apexes of enterocytes in the basal area and intracellularly in inflammatory tissue. In contrast, B(1)R protein is found in the basal area of enterocytes in normal intestine but in the apical portion of enterocytes in inflamed tissue. B(1)R protein is significantly increased in both active UC and CD intestines compared with controls. In patients with active UC, B(1)R mRNA is significantly higher than B(2)R mRNA. However, in inactive UC patients, the B(1)R and B(2)R mRNA did not differ significantly. Thus bradykinin receptors in IBD may reflect intestinal inflammation. Increased B(1)R gene and protein expression in active IBD provides a structural basis of the important role of bradykinin in chronic inflammation.     

B chromosomes in two fish species, genus Rhamdia (Siluriformes, Pimelodidae)

Specimens belonging to two fish species genus Rhamdia were cytogenetically analysed from seven localities in Brazil and Argentina. In addition to the 58 chromosomes of the basic karyotype, one to five metacentric B chromosomes were observed carrying conspicuous heterochromatic blocks on the distal regions of both chromosome arms. These B chromosomes are mitotically stable and, in the two best-sampled populations in R. hilarii (Lobo and 29 reservoirs), they showed frequency distributions fitting a binomial distribution, though Bs were more frequent in the latter. The presence of B chromosomes with the same appearance in R. quelen suggests an ancient origin for these B chromosomes, presumably prior to speciation from a common ancestor.     

山东省烟草根际土壤真菌多样性研究

为了有助于研究烟草根际土壤真菌与根茎病害发生程度的相互关系,文中对采自山东省6个地区的122份烟草根际土壤样品进行了真菌的分离和培养,采用形态学方法进行鉴定,并分析了菌群的多样性组成。从山东烟草根际土壤中分离和鉴定出真菌22属79种,其中接合菌门4属6种,子囊菌门1属1种,无性型真菌17属72种。优势真菌种群为青霉属(Penicillium)、木霉属(Trichoderma)、镰孢菌属(Fusarium)和曲霉属(As-pergillus),所占分离真菌总菌数的比例分别为22.39%、13.62%、12.69%和9.88%。6个采样地区间真菌菌群的多样性水平存在差异,其中诸城地区的多样性指数(H′=2.066 6)、均匀度指数(J=0.689 8)和丰富度指数(R=3.730 1)均最高。     

Relationships of Bradyrhizobium strains nodulating three Algerian Genista species

The Mediterranean world is the cradle for the diversification of a large number of plant species, including legumes belonging to the Tribe Genisteae. Nodule bacteria from three species of Genista legumes indigenous to northwestern Africa (G. ferox, G. numidica, G. tricuspidata) were sampled across a 150 km region of Algeria in order to investigate symbiotic relationships. Partial 23S rRNA sequences from 107 isolates indicated that Bradyrhizobium was the predominant symbiont genus (96% of isolates), with the remainder belonging to Rhizobium or Mesorhizobium. A multilocus sequence analysis on 46 Bradyrhizobium strains using seven housekeeping (HK) genes showed that strains were differentiated into multiple clades with affinities to seven species: B. canariense (17 isolates), B. japonicum (2), B. ottawaense (2), B. cytisi/B. rifense (9), ‘B. valentinum’ (5), and B. algeriense (11). Extensive discordance between the HK gene phylogeny and a tree for four loci in the symbiosis island (SI) region implied that horizontal transfer of SI loci has been common. Cases of close symbiont relationship across pairs of legumes hosts were evident, with 33% of isolates having as their closest relative a strain sampled from a different Genista species. Nevertheless, tree permutation tests also showed that there was substantial host-related phylogenetic clustering. Thus, each of the three Genista hosts utilized a measurably different array of bacterial lineages.     

Ticks Infesting Livestock on Farms in Western Sicily, Italy

The results of a 2-year survey on ticks infesting livestock on farms in Western Sicily, Italy, are reported. Several dogs living in the same area were also examined for ticks. Moreover, free-living ticks from one cattle farm were caught by CO₂ traps and flagging. A total of 6208 specimens was collected belonging to 9 species: Rhipicephalus bursa (32.4%), Rhipicephalus turanicus (22.7%), Rhipicephalus sanguineus (19.3%), Hyalomma lusitanicum (12.0%), Haemaphysalis punctata (7.8%), Hyalomma marginatum (3.5%), Dermacentor marginatus (1.4%), Ixodes ricinus (0.8%) and Ixodes hexagonus (0.1%). The species belonging to the genus Rhipicephalus were present on all hosts and habitats monitored, R. bursa was found to be abundant on cattle (46.3%) and sheep (62.0%), R. sanguineus was prevalent on dogs (50.4%), and R. turanicus on donkeys (41.0%) and as free-living species (48.5%).     

Variation of Phytoplankton in a Tropical Estuary (Vellar Estuary,Bay of Bengal,India)

The phytoplankton species composition and community structure were studied at two stations in the Vellar estuary from January to December, 1983. A total of 117 species of phytoplankton belonging to various groups, namely diatoms (90), dinoflagellates (21), Chlorophyceae (3), Cyanophyceae (2) and silicoflagellates (1) were observed during the study. The species diversity, richness and evenness values varied from 0.7 to 4.2 units/individual, 0.9 to 5.7 and 0.19 to 0.75 respectively. Dominance index values varied between 32.6 and 96.4 during the study period.     

Phylogeny and genetic diversity of native rhizobia nodulating common bean (Phaseolus vulgaris L.) in Ethiopia

The diversity and phylogeny of 32 rhizobial strains isolated from nodules of common bean plants grown on 30 sites in Ethiopia were examined using AFLP fingerprinting and MLSA. Based on cluster analysis of AFLP fingerprints, test strains were grouped into six genomic clusters and six single positions. In a tree built from concatenated sequences of recA, glnII, rpoB and partial 16S rRNA genes, the strains were distributed into seven monophyletic groups. The strains in the groups B, D, E, G1 and G2 could be classified as Rhizobium phaseoli, R. etli, R. giardinii, Agrobacterium tumefaciens complex and A. radiobacter, respectively, whereas the strains in group C appeared to represent a novel species. R. phaseoli, R. etli, and the novel group were the major bean nodulating rhizobia in Ethiopia. The strains in group A were linked to R. leguminosarum species lineages but not resolved. Based on recA, rpoB and 16S rRNA genes sequences analysis, a single test strain was assigned as R. leucaenae. In the nodC tree the strains belonging to the major nodulating groups were clustered into two closely linked clades. They also had almost identical nifH gene sequences. The phylogenies of nodC and nifH genes of the strains belonging to R. leguminosarum, R. phaseoli, R. etli and the putative new species (collectively called R. leguminosarum species complex) were not consistent with the housekeeping genes, suggesting symbiotic genes have a common origin which is different from the core genome of the species and indicative of horizontal gene transfer among these rhizobia.     

Infectivity of Echinostoma friedi miracidia to different snail species under experimental conditions

The infectivity of Echinostoma friedi (Trematoda: Echinostomatidae) miracidia was studied experimentally in a range of laboratory-reared snails that coexist in the same natural locality, namely Radix peregra, Lymnaea fuscus, L. truncatula (Lymnaeidae), Gyraulus chinensis, Helisoma duryi (Planorbidae) and Physella acuta (Physidae), and snails from different geographical origins acting naturally or experimentally as intermediate hosts of Schistosoma spp., namely Planorbarius metidjensis (from Málaga, Spain), Biomphalaria glabrata (Guadeloupe), B. alexandrina (Egypt) (Planorbidae), Bulinus cernicus (Mauritius), B. globosus (Zambia), B. natalensis (South Africa) and B. truncatus (Niger) (Bulinidae). Six species of snails were found to be susceptible, with the rate of infection ranging from 0 to 36.7%. The highest infection was detected in R. peregra. The low host specificity of E. friedi might have an epidemiological significance as a requisite for a recent establishment in a new geographical area.     

四川七种蝙蝠的核型

本报道了四川地区7种蝙蝠的核型。菊头蝠科2种,即角菊头蝠(Rhinolophus comutus)的核型为2n=62,FN=60;栗黄菊头蝠(R．ssinicus)的核型为2n=36,FN=60。蹄蝠科1种,即大蹄蝠(H．armiger)的核型为2n=32,FN=60。蝙蝠科3种,即伏翼(Pipistrellus abramns)的核型为2n=26,FN=44;南蝠(Iaio)的核型为2n=50,FN=48;山蝠(Nyctalus velutinus)的核型为2n=36,FN=50。犬吻蝠科的皱唇蝠(Tadarida teniotis)的核型为2n=48,FN=62。南蝠的核型为首次报道,但栗黄菊头蝠的核型2n=36与安徽张维道报道相同,而与印度和斯里兰卡同种R．rouxii的核型2n=56迥异。分布在中国的栗黄菊头蝠R．sinicus应为独立种而不是R．rouxii的中国亚种。另5种蝙蝠的核型与前人研究结论基本一致。