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1.
Two novel ascomycetous yeast species, Saturnispora serradocipensis and Saturnispora gosingensis, were isolated from leaf detritus in a tropical stream of Southeastern Brazil and a mushroom collected in Taiwan, respectively.
Analysis of the D1/D2 domains of the large-subunit of the rRNA gene of these strains showed that these species are related
to Saturnispora hagleri, their closest relative. Saturnispora serradocipensis and S. gosingensis differed from S. hagleri, respectively, by seven nucleotide substitutions and two indels and three nucleotide substitutions and three indels in D1/D2
rRNA sequences. The two new species differ from each another by four nucleotide substitutions and one indel in D1/D2 rRNA
sequences. However, the ITS sequences of S. serradocipensis, S. gosingensis and S. hagleri were quite divergent, showing that they are genetically separate species. The type strain of S. serradocipensis is UFMG-DC-198 T (=CBS 11756 T = NRRL Y-48717 T), and of S. gosingensis GA4M05 T is (CBS 11755 T = NRRL Y-48718 T). 相似文献
2.
Anaerobic enrichments at pH 10, with pectin and polygalacturonates as substrates and inoculated with samples of sediments
of hypersaline soda lakes from the Kulunda Steppe (Altai, Russia) demonstrated the potential for microbial pectin degradation
up to soda-saturating conditions. The enrichments resulted in the isolation of six strains of obligately anaerobic fermentative
bacteria, which represented a novel deep lineage within the order Clostridiales loosely associated with the family Lachnospiraceae. The isolates were rod-shaped and formed terminal round endospores. One of the striking features of the novel group is a
very narrow substrate spectrum for growth, restricted to galacturonic acid and its polymers (e.g. pectin). Acetate and formate
were the final fermentation products. Growth was possible in a pH range from 8 to 10.5, with an optimum at pH 9.5–10, and
in a salinity range from 0.2 to 3.5 M Na +. On the basis of unique phenotypic properties and distinct phylogeny, the pectinolytic isolates are proposed to be assigned
to a new genus Natranaerovirga with two species N. hydrolytica (APP2 T=DSM24176 T=UNIQEM U806 T) and N. pectinivora (AP3 T=DSM24629 T=UNIQEM U805 T). 相似文献
3.
Five strains (LN12, LN14 T, LN15 T, LN16 and LN17 T) representing three novel methylotrophic yeast species were isolated from the external surface of plant leaves by three-consecutive
enrichments. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, the sequence analysis
of the D1/D2 domain of the large subunit (LSU) rRNA gene and the phylogenetic analysis, the five strains were assigned to
be one novel Ogataea species and two novel Candida species. Three strains (LN12, LN14 T and LN16) represent a single novel species of the genus Ogataea, for which the name Ogataea phyllophila sp. nov. is proposed. The type strain is LN14 T (= BCC 42666 T = NBRC 107780 T = CBS 12095 T). Strain LN15 T was assigned to be Candida chumphonensis sp. nov. (type strain LN15 T = BCC 42667 T = NBRC 107781 T = CBS 12096 T). Strain LN17 T represented another novel species of Candida that was named Candida mattranensis sp. nov. (type strain LN17 T = BCC 42668 T = NBRC 107782 T = CBS 12097 T). 相似文献
4.
Three strains (LM008 T, LM068 and LM078 T), representing two novel yeast species were isolated from the phylloplane of three plant species by an enrichment technique. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and the sequence analysis of the D1/D2 domain of the large subunit rRNA gene and the internal spacer region, the three strains were assigned as two novel Candida species. Strain LM008 T was assigned to be Candida sirachaensis sp. nov. (type strain LM008 T = BCC 47628 T = NBRC 108605 T CBS 12094 T) in the Starmerella clade. Two strains (LM068 and LM078 T) represent a single species in the Lodderomyces- Spathaspora clade for which the name Candida sakaeoensis sp. nov. is proposed with the type strain LM078 T = BCC 47632 T = NBRC 108895 T = CBS 12318 T. 相似文献
5.
Parsimony network analysis of rDNA sequences was used to delimit phylogenetic species of yeasts in an objective, formal manner.
Many strains assigned to Candida apicola ( Starmerella clade), when compared to the type, fell outside the inclusion limits proposed by Kurtzman and Robnett ( 1998) based on a pair-wise comparison of the large subunit rRNA gene D1/D2 domains. However, when these sequences were analyzed
jointly with ITS rDNA sequences by parsimony network analysis, 28 of the 30 strains formed a cohesive set. Two strains, MUCL
45721 and CBS 4353, were excluded from the species, but there was no evident justification to subdivide the rest. A similar
analysis of 81 isolates originally assigned to Candida azyma ( Wickerhamiella clade) yielded dramatically different results, giving rise to six independent networks corresponding to Candida azyma sensu stricto (18 strains), Candida azymoides (2 strains), a pair of isolates from Australian hibiscus flowers, a single isolate from the same substrate, a single isolate
from Malaysian bertam palm nectar, and 57 isolates that are assigned to the new species Candida parazyma (type = UWOPS 91-652.1 T = CBS 11563 T = NRRL Y-48669 T). The strains retained in C. azyma sensu stricto differed from one another by up to four substitutions in their D1/D2 sequences, but their polymorphism at the
level of the ITS was considerable and suggested a history of divergence resulting from dispersal. Strains of C. parazyma fell into seven variant haplotypes based on sequences of the rDNA ITS and D1/D2 regions. The most abundant haplotype occurred
across the global range of the species. Others were either endemic to Belize, Costa Rica, Rarotonga, or Tennessee, suggestive
of vicariance, or occurred across remote localities, offering partial support to the notion of rapid dispersal. 相似文献
6.
During a survey of yeasts associated with raw honey collected in Thailand, two strains of the Zygoascus clade were isolated from the Asian cavity-nesting honeybee Apis cerana and the stingless bee Homotrigona fimbriata. Phylogeny based on 26S rDNA D1/D2 sequences placed these yeasts as members of a clade including Candida bituminiphila, Candida patagonica and Candida polysorbophila. The strains of the two novel species, CBS 12271 T and CBS 12270 T, respectively, could be unquestionably distinguished from their relatives by rDNA sequences and other taxonomic characteristics.
Therefore, the novel anamorphic species, Candida lundiana sp. nov. (type strain CBS 12271 T = JCM 16823 T) and Candida suthepensis sp. nov. (type strain CBS 12270 T = JCM 16822 T) are described. 相似文献
7.
In a taxonomic study of yeasts isolated from decaying plant materials submerged in water of mangrove forests in Thailand,
three strains isolated from tree bark (EM33 T), a fallen leaf (EM40 T) and a detached branch (SM56 T) were found to represent three novel yeast species. On the basis of morphological, biochemical, physiological and chemotaxonomic
characteristics, the sequence analysis of the D1/D2 domain of the large subunit (LSU) rRNA gene, and the phylogenetic analysis,
the three strains were assigned as three novel Candida species. They were named as Candida chanthaburiensis sp. nov. (type strain EM33 T = BCC 23057 T = NBRC 102176 T = CBS 10926 T), Candida kungkrabaensis sp. nov. (type strain EM40 T = BCC 23060 T = NBRC 102179 T = CBS 10927 T), and Candida suratensis sp. nov. (type strain SM56 T = BCC 25961 T = NBRC 103858 T = CBS 10928 T). 相似文献
9.
Among the basidiomycetous yeasts isolated from plant leaves collected in different regions of China, two ballistoconidium-forming
strains were revealed to represent an undescribed species of the genus Bensingtonia by conventional, chemotaxonomic and molecular phylogenetic characterization. Sequence analysis of the 26S rDNA D1/D2 domains
and the internal transcribed spacer (ITS) region indicated that the novel species was located in the Agaricostilbum lineage
and closely related to Bensingtonia naganoensis and Bensingtonia ciliata, with the former as its closest relative. The name Bensingtonia pseudonaganoensis sp. nov. is proposed (type strain: AS 2.2601 T = CBS 10121 T) 相似文献
10.
The six synonyms currently accepted under Saccharomycodes ludwigii were investigated for by phenotypic properties, however, the sequence diversity of the rRNA and protein coding genes have not yet been determined. Nine strains including the type strains of synonyms of S. ludwigii deposited in the CBS yeast collection, Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands, were analyzed using a multi-locus sequence analysis (MLSA) approach that included sequences of 18S ribosomal RNA (rRNA), the D1/D2 domains of the 26S rRNA, the ITS region (including the 5.8S rRNA) and fragments of genes encoding the largest subunit of the RNA polymerase II (RPB1 and RPB2) and translation elongation factor 1-α (TEF1). Our results showed that the nine strains have identical D1/D2, 18S and RPB2 sequences and similar ITS, RPB1 and TEF1 sequences, which indicated that they are conspecific. In addition, a novel species of Saccharomycodes, S. pseudoludwigii sp. nov. (type CGMCC 2.4526 T) that was isolated from fruit and tree bark in China, is proposed. The MycoBank number of this new species is MB 811,650. 相似文献
11.
A novel anamorphic yeast strain, A1-01 T, belonging to the genus Rhodotorula was isolated from a plant in Taiwan and analysed morphologically, physiologically and phylogenetically. Neither ballistoconidia
nor sexual reproduction was observed. Sequence analysis of the 26S rRNA gene and the ITS region indicate that Rhodosporidium sphaerocarpum is the most closely related species, with 14 and 24 nucleotide substitutions, respectively. The novel species differed physiologically
from R. sphaerocarpum in its ability to assimilate ethylamine and cadaverine, its inability to assimilate ethanol and nitrite. From these comparative
analyses, the following novel yeast species is proposed: Rhodotorula taiwanensis sp. nov. with the type strain of A1-01 T (BCRC 23118 T = CBS 11729 T). 相似文献
13.
Nine strains of a new Torulaspora species were isolated from natural samples collected in Japan and Thailand including one strain obtained from a leaf of Rhizophora stylosa (NBRC 11061T), one strain from soil (NBRC 11062), six strains from mosses (ST-14, ST-266, ST-510, ST-511, ST-513 and ST-581) and one strain from sediment in mangrove forest (RV-51). On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and the sequence analyses of the D1/D2 domain of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) (ITS1-5.8S rRNA gene-ITS2) region, the nine strains were found to represent a single novel species of the genus Torulaspora, which were named Torulaspora maleeae sp. nov. The type strain is NBRC 11061T (BCC 25515T=CBS 10694T). In the phylogenetic trees based on the sequences of the D1/D2 domain of the LSU rRNA gene, T. maleeae showed a close relationship with the five recognized species of the genus Torulaspora, Torulaspora delbrueckii, Torulaspora franciscae, Torulaspora globosa, Torulaspora microellipsoides and Torulaspora pretoriensis. Torulaspora maleeae differed from the five recognized species of the genus Torulaspora by six to 12 nucleotide substitutions (1.1-2.1%) in the D1/D2 domain of the LSU rRNA gene and by 6.4-11.7% nucleotide substitutions in the ITS (ITS1-5.8S rRNA gene-ITS2) region. 相似文献
14.
Three hundred and thirty-seven xylose-utilizing yeast strains were isolated from various natural samples. Among these, 68 strains produced xylitol in the range of 0.1–0.69 g xylitol/g xylose. Thirty-nine xylitol-producing strains were identified to be Candida tropicalis. Ten strains were found belonging to 14 known species in the genus Candida, Cyberlindnera, Meyerozyma, Pichia, Wickerhamomyces, Yamadazyma and Cryptococcus. Two strains were identified to be two Candida species and two strains (DMKU-XE142 T and DMKU-XE332) were found to be a novel species. Strain DMKU-XE142 T was isolated from tree bark and DMKU-XE332 was obtained from decaying plant leaf collected in Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics and sequence analysis of the D1/D2 region of the large subunit rRNA gene (LSU) and the internal transcribed spacer (ITS) region, the two strains were determined to represent a novel Yamadazyma species although formation of ascospores was not observed. The sequences of the D1/D2 region of the LSU rRNA gene and the ITS region of the two strains were identical but differed from Yamadazyma phyllophila, the closest species in terms of pairwise sequence similarity of the D1/D2 region, by 1.7 % nucleotide substitutions and 3.5 % nucleotide substitutions in the ITS region. The name Yamadazyma ubonensis f.a., sp. nov. is proposed (type strain is DMKU-XE142 T = BCC 61020 T = CBS 12859 T). 相似文献
15.
Three ascosporogenous yeast strains were isolated from the gut of the passalid beetle, Odontotaenius disjunctus, inhabiting on rotten oak trees. DNA sequence comparison and other taxonomic characteristics identified the strains as a
novel species in the genus Kazachstania. The name Kazachstania
intestinalis sp. nov. (type strain EH085 T = ATCC MYA-4658 T = CBS 11839 T) is proposed for the strains. The yeast is homothallic, producing persistent asci with 1–4 spheroidal ascospores. Molecular
phylogeny from ribosomal RNA gene sequences placed this novel species on the basal lineage of a clade including Kazachstania lodderae, Kazachstania exigua, Kazachstania martiniae, and other related Kazachstania spp., but none of those species was a close sister to K.
intestinalis. 相似文献
16.
A new ascomycetous yeast species, Candida neustonensis is proposed in this study based on four strains (SN92 T, SN47, SJ22, SJ25) isolated from sea surface microlayer in Taiwan. These four yeast strains were morphologically, physiologically
and phylogenetically identical to each other. No sexual reproduction was observed on 5% malt extract agar, corn meal agar,
V8 agar, McClary’s acetate agar and potato-dextrose agar. Phylogenetic analysis of the sequences of the D1/D2 domain of the
large subunit (LSU) rRNA gene places C. neustonensis as a member of the Pichia guilliermondii clade, it also reveals that the phylogenetically closest relatives of C. neustonensis are C. fukuyamaensis (4.4% divergence), C. xestobii (4.4% divergence) and P. guilliermondii (4.5% divergence). C. neustonensis also is clearly distinguished from other known species in the P. guilliermondii clade based on the results of physiology tests. From these comparison analyses, the following novel yeast species is proposed:
Candida neustonensis sp. nov., with strain SN92 T (= BCRC 23108 T = JCM 14892 T = CBS 11061 T) as the type strain. 相似文献
17.
Ten strains of a new endophytic ascospore-forming, methanol-assimilating yeast were isolated from the galls induced by sawflies
on the leaves of willows in the Losiny Ostrov National Park (Moscow region). Standard phenotypical tests and phylogenetic
analyses of 18S rRNA gene, 5.8S-ITS gene region and 26S rRNA gene (D1/D2 domains) sequences showed that the species belongs
to the genus Ogataea. We describe it as Ogataea cecidiorum and designate type culture KBP Y-3846 (= CBS 11522 T = VKM Y-2982 T = VKPM Y-3482 T = MUCL 52544 T = NCAIM Y.01965 T) as the type strain. The new species was registered in MycoBank under MB 515233. 相似文献
18.
Two novel species of the genus Deinococcus, designated SYSU M49105T and SYSU M42101T, were isolated from freshwater samples of the Pearl River estuary in Guangdong, China. Phylogenetic analysis using 16S rRNA gene sequence indicated that strains SYSU M49105T and SYSU M42101T showed the highest sequence similarities to Deinococcus aetherius JCM 11751 T (93.6%) and Deinococcus multiflagellatus NBRC 112888 T (97.3%), respectively. Cells of both strains were Gram-staining positive, aerobic, coccus-shaped, oxidase-negative and non-motile. The cell wall contained meso-diaminopimelic acid as their diagnostic diamino acid. MK-8 was the predominant respiratory quinone for both strains. The polar lipid profile of SYSU M49105T contained two unidentified phosphoglycolipids, nine unidentified glycolipids, and five unidentified polar lipids. SYSU M42101T had one unidentified phosphoglycolipid, nine unidentified glycolipids, one unidentified aminophospholipid and four unidentified polar lipids. The major fatty acids of strains SYSU M49105T and SYSU M42101T were summed feature 3 (C16:1 ω7c and/ or C16:1 ω6c) and C16:0. The G?+?C contents of the novel isolates based on genomic DNAs were 69.6% and 67.4%, respectively. On the basis of phenotypic, genotypic and phylogenetic data, strains SYSU M49105T and SYSU M42101T should be considered to represent two novel species in the genus Deinococcus, for which the names Deinococcus aestuarii sp. nov. and Deinococcus aquaedulcis sp. nov. were proposed with the type strains SYSU M49105T (=?KCTC 43258 T?=?CGMCC 1.18609 T) and SYSU M42101T (=?KCTC 43257 T?=?CGMCC 1.18614 T), respectively. 相似文献
19.
Two yeast morphotypes, BET 4 T and BET 7, were isolated from the gut of click beetle Melanotus villosus. Click beetles were collected from the decaying timber within the woodlands of North Wyke Research, South West England, UK
(latitude, 50°46′29″N; longitude, 3°55′23″W). Morphotype BET 7 was identified as Debaryomyces hansenii, and the other morphotype, BET 4 T, was found to differs from Priceomyces castillae and Priceomyces haplophilus, its closest phylogenetic neighbours, by 5.0% with respect to the nucleotide sequence of the D1/D2 domain of the large-subunit
(LSU) rRNA gene, and by 8.0% with respect to the ribosomal internal-transcribed spacer (ITS) region. BET 4 T also differ from P. castillae and P. haplophilus in a number of different phenotypic characteristics. Thus, based on the unique nucleotide sequences of its D1/D2 domain and
ITS region, its physiological characteristics and an inability to sporulate, strain BET 4 T is assigned the status of a new species of Candida, for which the name Candida northwykensis sp. nov., is proposed. The type strain is BET 4 T (NCYC 3525 T = CBS 11370 T). 相似文献
20.
Five yeast morphotypes were isolated from biogas reactors at North Wyke Research, Okehampton, UK. Out of the five morphotypes,
four were identified as known species. In contrast, the fifth morphotype strain, Bio10 T, was found to differ from Bullera dendrophila and Kwoniella mangroviensis, its closest phylogenetic neighbours, by 2.6–2.9% with respect to the nucleotide sequence of the D1/D2 domain of the 26S
rRNA gene and by 5.6–6.2% with respect to the internal transcribed spacer 1 (ITS1)-5.8S rRNA gene-ITS2 region. Bio10 T also differs from these two species by a number of phenotypic characteristics. Thus, based on the phenotypic differences
and phylogenetic analysis, strain Bio10 T is assigned the status of a new species of Cryptococcus, for which the name Cryptococcus
shivajii sp. nov. is proposed. The type strain is Bio10 T (NCYC 3541 T = CBS 11374 T). 相似文献
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